Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT)  murN → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT100.0% 152.4 / NA 50T15T (ACA→ACT) murNFemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/31);  total (19/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAG  >  CP000730/2532163‑2532431
                                                                                                                                       |                                                                                                                                     
ggtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACaaa                                                                                                                                  <  1:199000/140‑1 (MQ=255)
 gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATCGAATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                 <  1:85110/141‑1 (MQ=255)
 gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                 <  1:256043/141‑1 (MQ=255)
 gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                 <  1:206220/141‑1 (MQ=255)
  tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                <  1:14210/141‑1 (MQ=255)
      tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                            <  1:28678/141‑1 (MQ=255)
           tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTCCTACAAAGCATTTTGcac                                                                                                                       >  2:321585/1‑141 (MQ=255)
                ttttGATAAAATAAAAAAATAGCATTATTAATGAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAAGGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACCTTac                                                                                                                  >  1:331611/1‑141 (MQ=255)
                ttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTac                                                                                                                  >  1:250955/1‑141 (MQ=255)
                       aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAt                                                                                                           <  1:423198/141‑1 (MQ=255)
                            aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                      <  2:389172/141‑1 (MQ=255)
                            aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                      <  1:221756/141‑1 (MQ=255)
                             aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTa                                                                                                     <  1:31789/141‑1 (MQ=255)
                              aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACACACAGCtgg                                                                                                    <  2:119742/141‑3 (MQ=255)
                              aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt                                                                                                    <  1:176823/141‑1 (MQ=255)
                                     gCATTATTAATAACTCCAAACATCATAGTCCAATTCAGAGAGGGATGGGATAAAATGAACTTTCTAACGTTGACTTCGCATGAGTTCACTGCGCCTCCTACACACCATCTTCCACATCCCACCCAATCCGccccccactcc                                                                                             >  2:293425/1‑131 (MQ=255)
                                       attattAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACaa                                                                                           >  1:328242/1‑141 (MQ=255)
                                                 aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                                 <  1:388263/141‑1 (MQ=255)
                                                        aaaTTATAGTTCAATTCAGAGATGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                          >  2:337081/1‑141 (MQ=255)
                                                        aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                          >  1:414336/1‑141 (MQ=255)
                                                          aTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaaa                                                                        >  1:107498/1‑141 (MQ=255)
                                                             ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGGGTTGATTTAAAAGGc                                                                     >  1:291745/1‑141 (MQ=255)
                                                              gagtgcgagtcggagggGGGTGGGAGAGAGTGAACTGTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                    <  1:293425/129‑1 (MQ=255)
                                                                gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                  >  1:383201/1‑141 (MQ=255)
                                                                gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                  >  1:369303/1‑141 (MQ=255)
                                                                gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                  <  2:102169/141‑1 (MQ=255)
                                                                 ttCAATTCAGGCGGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGGATTTTTCACAGTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                 <  2:127747/141‑1 (MQ=255)
                                                                 ttCAATTCAGAGAGGGATGGGATAAAATGAACTATGTAACGTTGACTTCGGATTACTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACGATCAGCTATTCATTACAATCATACAGTTGATTTAAAAGGCGAtg                                                                 <  2:252868/141‑1 (MQ=255)
                                                                   cAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgtg                                                               <  1:271873/141‑1 (MQ=255)
                                                                    aaTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGc                                                              <  1:158599/141‑1 (MQ=255)
                                                                     atgcAGAGAGCGATGGGAGAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCa                                                             <  2:284234/138‑1 (MQ=255)
                                                                       tCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                           >  1:156825/1‑141 (MQ=255)
                                                                         agagagGGTTGCGCTACAATGCACTGTGCTACCTTGACTTGGGATGAGTTCTTTGCGTGGACTACAAAGCATTTTGCACTTTACACACAATCGGCTATTCATTACAAGCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                         <  2:89851/141‑1 (MQ=255)
                                                                         agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGGTTACTACAAAGCATTTTTCACATTACACACAAGCAGCTAACACATACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                         <  1:220863/141‑1 (MQ=255)
                                                                         agagagGGAGGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                         <  2:29460/141‑1 (MQ=255)
                                                                          gatggggatgggagAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                        <  2:200359/137‑1 (MQ=255)
                                                                          gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                        >  2:142736/1‑141 (MQ=255)
                                                                            gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTa                                                      >  2:411148/1‑141 (MQ=255)
                                                                               ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTAACAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGCTTTAAAAGGCGATGTGCATCTTGGAggg                                                   >  2:126592/1‑141 (MQ=255)
                                                                                 atgggatAAAATGAACTTTGTAGCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                 <  2:240365/141‑1 (MQ=255)
                                                                                 atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                 >  2:52007/1‑141 (MQ=255)
                                                                                                    gTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACCCTCAGCCATTCATTACAATCATAGAGTTGATTTCAAAGGCAATGTGCATCTTCTAGGGGTTAACGATcacacgggccca                              >  2:150642/1‑133 (MQ=255)
                                                                                                         tgtGACTTCGGATGAGTTCAATGCGTTTACTACTAAGCATTTATAAAATTAAACACAATCAGCTATTCATTACAATCATAGAGTTGACTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                         <  1:267360/139‑1 (MQ=255)
                                                                                                         gTTGACTTCGGATGATTTCAATGCGTTTACTACAAAGCATTTTTCATATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                         <  2:197808/141‑1 (MQ=255)
                                                                                                                 cGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAGTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                 >  1:454831/1‑141 (MQ=255)
                                                                                                                  ggATGAGTTCAATGCGTTTACTACAATGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAGGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGagt                <  2:402183/141‑3 (MQ=255)
                                                                                                                       aGTTCAATGCGTTTACTACAAAGCATTTTTCACCTTACACACAATCAGCTATTCATTGCAATCTAAGCGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATTACAATGGTCAAGTCATTGCAGGCTGCTTAt           >  2:51070/1‑141 (MQ=255)
                                                                                                                           cAATGCGTTTACTACAAAGCATTTTTCACATTACACTCAAGTATCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAc       <  1:84895/141‑1 (MQ=255)
                                                                                                                                cGTTTACTACAATGCATTTTTCACATTACACACAATCAGCTATGCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg  <  1:36226/141‑1 (MQ=255)
                                                                                                                                cGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg  <  1:90845/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAG  >  CP000730/2532163‑2532431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑