Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 16.2% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC16.2% 65.1 / 10.6 39intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (19/13);  new base C (4/2);  total (24/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01

AGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCT  >  CP000730/76158‑76420
                                                                                                                                         |                                                                                                                             
aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTGACAAGGACGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                                                                                                                            >  1:341056/1‑141 (MQ=25)
 ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGACTACTATCTAAGATATGCATTGAGTTATc                                                                                                                           >  1:30020/1‑141 (MQ=21)
       tttacAAAGTTGAATCTATAATAAAAATAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                                                                                                                     <  2:423550/137‑1 (MQ=11)
       gTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                                                                                                                     <  1:378014/141‑1 (MQ=35)
       cgttcAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                                                                                                                     <  2:225883/137‑1 (MQ=25)
           cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCTTTATAGATc                                                                                                                 >  2:393187/1‑141 (MQ=255)
            aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCt                                                                                                                >  2:436742/1‑141 (MQ=35)
             aaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg                                                                                                               <  1:277130/141‑1 (MQ=35)
                    aTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATGTGAAATAtt                                                                                                        >  1:391156/1‑141 (MQ=21)
                      ttataatataaTTATAACAAAAAGGAGTCTACTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                      <  2:293384/140‑1 (MQ=255)
                         taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGACAAATTCAggg                                                                                                   >  1:378573/1‑141 (MQ=12)
                           gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTAATCGTGATATATCTGAAATATTAAGGGaa                                                                                                 >  1:98799/1‑141 (MQ=17)
                           gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGAATGTTATCACTGTAGCCGTTGGCTACTAACTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGaa                                                                                                 >  1:98814/1‑141 (MQ=17)
                             aaaaTTTTAACAAAAAGGAGTCTTCTGTAAGAACTAATACAAATAAAAACAATTAAACAAGGATGTTATCACTGTAGCCGTTGGCTACAATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                                               <  2:8243/139‑1 (MQ=11)
                              taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgt                                                                                              >  1:181296/1‑141 (MQ=17)
                                aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                                                                            <  2:431278/141‑1 (MQ=32)
                                                 tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAACCGTTCATCAtt                                                                           >  1:448916/1‑141 (MQ=17)
                                                             aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTGCTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                               <  1:258712/141‑1 (MQ=17)
                                                                  ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                                          <  1:436782/141‑1 (MQ=32)
                                                                    cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATAAACATATATGTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGt                                                        >  1:107080/1‑141 (MQ=11)
                                                                      gATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAAATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCCACGATCTACCGTAGGGTTc                                                      >  1:206051/1‑141 (MQ=11)
                                                                           aaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGaa                                                 >  2:284445/1‑141 (MQ=32)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTCCGATATGATTTGGAATATCGTGATATATCTGAAATATGAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTTTACCGTTGGCTTTAAGAATATGCACCaa                                       >  1:271973/1‑141 (MQ=11)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCTCCaa                                       >  1:285521/1‑141 (MQ=17)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCACCaa                                       >  2:390706/1‑141 (MQ=17)
                                                                                      cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAt                                      >  2:323664/1‑141 (MQ=32)
                                                                                      cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAt                                      >  1:269993/1‑141 (MQ=32)
                                                                                        aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATAACTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACTGTCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                    >  1:25148/1‑141 (MQ=17)
                                                                                             gTTATCGCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                               <  2:417626/141‑1 (MQ=25)
                                                                                               tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                             >  2:38302/1‑141 (MQ=35)
                                                                                               tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                             <  1:428579/141‑1 (MQ=35)
                                                                                                      gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                      <  1:331602/141‑1 (MQ=25)
                                                                                                          ccGTTGGCTACTATCTAAGATTTGCAATGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                  <  1:241744/141‑1 (MQ=21)
                                                                                                            gTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                >  1:365246/1‑141 (MQ=255)
                                                                                                            aacggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                >  1:463146/4‑141 (MQ=255)
                                                                                                              tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa              >  1:34335/1‑141 (MQ=35)
                                                                                                               ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg             <  1:436742/141‑1 (MQ=35)
                                                                                                                gctaacaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc            <  1:15377/135‑1 (MQ=25)
                                                                                                                          aaGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCACATTTGGAAGAAAAAGCATAAAAAGCt  >  1:156301/1‑141 (MQ=38)
                                                                                                                                         |                                                                                                                             
AGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCT  >  CP000730/76158‑76420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: