Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,274,220	A→G	D208G (GAT→GGT)	codY →	CodY family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,274,220	0	A	G	100.0%	122.2 / NA	34	D208G (GAT→GGT)	codY	CodY family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

AAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTCTTAGATGAATTAGAAA  >  CP000730/1274087‑1274360                                                                                                                                      |                                                                                                                                             aaaGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTa                                                                                                                                >  1:95896/1‑148 (MQ=255) aaaGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGGATTa                                                                                                                                >  1:202288/1‑148 (MQ=255)       gcgcgcGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGAt                                                                                                                          <  1:105535/148‑1 (MQ=255)       gcgcgcGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGAt                                                                                                                          >  1:109744/1‑148 (MQ=255)            cgATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTa                                                                                                                     >  2:122249/1‑148 (MQ=255)                               aTGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTa                                                                                                  >  2:120945/1‑148 (MQ=255)                                            cATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGc                                                                                     <  2:239499/148‑1 (MQ=255)                                             aTTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCt                                                                                    <  2:146406/148‑1 (MQ=255)                                               tATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTgg                                                                                  <  1:24799/148‑1 (MQ=255)                                               tATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTgg                                                                                  <  1:188497/148‑1 (MQ=255)                                                           aaaaaGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATc                                                                      >  2:232389/1‑148 (MQ=255)                                                             aaaGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCAc                                                                    >  1:196066/1‑148 (MQ=255)                                                             aaaGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTAAGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCAc                                                                    >  1:239345/1‑148 (MQ=255)                                                                     gATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTa                                                            <  1:84810/148‑1 (MQ=255)                                                                              tatCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGaaa                                                   <  1:254241/148‑1 (MQ=255)                                                                                    tGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACt                                             >  2:23116/1‑148 (MQ=255)                                                                                    tGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACt                                             >  2:168315/1‑148 (MQ=255)                                                                                     gaagaaCTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACtt                                            <  2:296939/148‑1 (MQ=255)                                                                                              ggCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAg                                   <  1:122249/148‑1 (MQ=255)                                                                                                  gTACGGAAGGCCTATTAATCGCATCAAAAGGTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTaa                               <  2:180335/148‑1 (MQ=255)                                                                                                             cTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAAt                    <  2:148915/148‑1 (MQ=255)                                                                                                             cTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAAt                    <  2:111629/148‑1 (MQ=255)                                                                                                             cTATTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAATAAAAAAAGAAAAAt                    <  2:111661/148‑1 (MQ=255)                                                                                                               aTTAATCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTc                  >  2:298038/1‑148 (MQ=255)                                                                                                                    tCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTCTTAGa             >  2:188445/1‑148 (MQ=255)                                                                                                                    tCGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTCTTAGa             <  2:34591/148‑1 (MQ=255)                                                                                                                     cGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTCAAGTTAAAAACGAAAAATACTTAGAt            >  2:145014/1‑148 (MQ=255)                                                                                                                     cGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTCTTAGAt            >  1:196235/1‑148 (MQ=255)                                                                                                                     cGCATCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATAACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTATTAGAt            >  1:229550/1‑148 (MQ=255)                                                                                                                        aTCAAAAGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTCTTAGATGaa         >  2:263123/1‑148 (MQ=255)                                                                                                                        aTCAAAAGTTGCAGGTAGAGTTGGTATTACTACATCAGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACATTCATTAAAGTTAAAAAAGAAAAATCCTTAGATGaa         >  2:77573/1‑148 (MQ=255)                                                                                                                        aTCAAAAGTTGCAGGTAGAATTAAAAAAAATAAAACTGTAATAGTAAATGAACTACGTAAATAAGAAAGTGCTGGTGAAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAAAAAAAAAAAAAAAATTCTTAGATGaa         <  2:26772/148‑1 (MQ=255)                                                                                                                           aaaaGTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTCTTAGATGAATTa      <  1:87901/148‑1 (MQ=255)                                                                                                                               gTTGCAGGTAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTCTTAGATGAATTAGaaa  >  1:61552/1‑148 (MQ=255)                                                                                                                                      |                                                                                                                                             AAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTCTTAGATGAATTAGAAA  >  CP000730/1274087‑1274360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑