Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,522,796	T→A	K171I (AAA→ATA)	dnaD1 ←	DNA replication protein DnaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,522,796	0	T	A	100.0%	53.1 / NA	17	K171I (AAA→ATA)	dnaD1	DNA replication protein DnaD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (7/10);  total (7/10)

ATCAAATTTAGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTTTATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGAGATAACGGCCTTGCAAATGTATCCTCTAAAACTCTAAATAA  >  CP000730/1522654‑1522935                                                                                                                                               |                                                                                                                                            aTCAAATTTAGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTc                                                                                                                                        >  1:136788/1‑148 (MQ=255)          aGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTc                                                                                                                               <  1:273548/148‑1 (MQ=255)                cGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCg                                                                                                                         <  1:73792/148‑1 (MQ=255)                               tgTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGACCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGt                                                                                                          <  2:184270/147‑1 (MQ=255)                               cGTCATTTTCGGTTTATTTAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGt                                                                                                          <  2:218700/148‑1 (MQ=255)                                gTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGta                                                                                                         >  1:263369/1‑148 (MQ=255)                                            ttATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCATATAATGCCGCTTGTATAATTGCAGTAt                                                                                             >  2:58456/1‑148 (MQ=255)                                                    tttttCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTa                                                                                     >  2:191705/1‑148 (MQ=255)                                                       ttCTCTTATTTCTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCa                                                                                  <  2:226937/148‑1 (MQ=255)                                                                     tAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTg                                                                    >  2:115962/1‑148 (MQ=255)                                                                          tCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTa                                                               <  2:249803/148‑1 (MQ=255)                                                                              cAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGt                                                           <  1:200422/148‑1 (MQ=255)                                                                                              gTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGga                                           <  1:266704/148‑1 (MQ=255)                                                                                                   tcttcCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGAGATaa                                      >  2:19926/1‑148 (MQ=255)                                                                                                                     tACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGAGATAACGGCCTTGCAAATGTATc                    >  2:111634/1‑148 (MQ=255)                                                                                                                                      aaGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGAGATAACGGCCTTGCAAATGTATCCTCTAAAACTCTAaata   <  2:137061/148‑1 (MQ=255)                                                                                                                                       aGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGAGATAACGGCCTTGCAAATGTATCCTCTAAAACTCTAaataa  <  2:136788/148‑1 (MQ=255)                                                                                                                                               |                                                                                                                                            ATCAAATTTAGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTTTATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGAGATAACGGCCTTGCAAATGTATCCTCTAAAACTCTAAATAA  >  CP000730/1522654‑1522935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑