Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,977,348	G→C	L368V (CTA→GTA)	sbcD ←	DNA repair exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,977,348	0	G	C	100.0%	95.9 / NA	27	L368V (CTA→GTA)	sbcD	DNA repair exonuclease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/15);  total (12/15)

TTCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTAGGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACTCATC  >  CP000730/1977204‑1977486                                                                                                                                                 |                                                                                                                                           ttCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGaa                                                                                                                                         <  2:4733/148‑1 (MQ=255)  ttaaTTTTACGTTGAACAGATTGACCGTATCCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTCGTATTTCTTCAGCACGATTAACTAATGCTGCATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                                        <  2:45740/146‑1 (MQ=255)  tCAATTTTACGTTGAACATATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                                        <  1:233800/148‑1 (MQ=255)  tCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                                        <  1:204668/148‑1 (MQ=255)  tCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                                        <  1:172131/148‑1 (MQ=255)   cAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACt                                                                                                                                       <  2:174540/148‑1 (MQ=255)     aTTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTa                                                                                                                                     >  2:38926/1‑148 (MQ=255)           cGTTGAACAAATTGACCGTAACCATACATTTCAAGTGATTTCATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGcc                                                                                                                               <  1:124057/148‑1 (MQ=255)                aaCAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTgg                                                                                                                          <  1:218222/148‑1 (MQ=255)                  cAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGAt                                                                                                                        <  2:142648/148‑1 (MQ=255)                             tAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATaaa                                                                                                             >  2:172634/1‑148 (MQ=255)                              aaCCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAAt                                                                                                            <  2:112748/148‑1 (MQ=255)                                                aTTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCaa                                                                                          >  1:140863/1‑148 (MQ=255)                                                      ttATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAg                                                                                    >  2:59987/1‑148 (MQ=255)                                                         tCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGttt                                                                                 >  1:213861/1‑148 (MQ=255)                                                         tCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGttt                                                                                 >  1:217487/1‑148 (MQ=255)                                                         tCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGttt                                                                                 >  1:213881/1‑148 (MQ=255)                                                                  tCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACta                                                                        >  1:10214/1‑148 (MQ=255)                                                                  tCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACta                                                                        >  1:193704/1‑148 (MQ=255)                                                                                                      cGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAACATTCAGTAACTAAAGGTGACt                                    >  2:273678/1‑148 (MQ=255)                                                                                                      cGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACt                                    >  2:189220/1‑148 (MQ=255)                                                                                                        aTTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACtca                                  <  2:117904/148‑1 (MQ=255)                                                                                                             cTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCAtt                             <  1:102593/148‑1 (MQ=255)                                                                                                                          gTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGt                <  1:109501/148‑1 (MQ=255)                                                                                                                                        aaTCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACtcatc  <  2:285517/148‑1 (MQ=255)                                                                                                                                        aaTCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACtcatc  >  2:192172/1‑148 (MQ=255)                                                                                                                                        aaTCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGAAAAAAAAAAAAAAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACtcatc  <  2:207656/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                           TTCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTAGGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACTCATC  >  CP000730/1977204‑1977486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑