Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,642,589	G→A	N18N (AAC→AAT)	ydaT ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,642,589	0	G	A	100.0%	75.7 / NA	24	N18N (AAC→AAT)	ydaT	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/9);  total (15/9)

GAACAACATCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTC  >  CP000730/2642447‑2642729                                                                                                                                               |                                                                                                                                             gAACAACATCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATtata                                                                                                                                         <  1:34199/148‑1 (MQ=255)   acaacaTCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCTTTTTTTCCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAAt                                                                                                                                       >  2:76467/1‑148 (MQ=255)         tCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGt                                                                                                                                 >  1:112442/1‑148 (MQ=255)         tCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGt                                                                                                                                 >  1:242036/1‑148 (MQ=255)                                tAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTTGGGGTAACTTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATaaaa                                                                                                          >  2:176837/1‑148 (MQ=255)                                tAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTGATGTTATATACCACCATAATTATaaaa                                                                                                          >  1:294130/1‑148 (MQ=255)                                      tGCATTCGCAATTTCTATCGCTTTTTTTCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAAc                                                                                                    >  2:110661/1‑148 (MQ=255)                                         aTTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTGATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACata                                                                                                 >  2:289118/1‑148 (MQ=255)                                            cGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTa                                                                                              <  2:30671/148‑1 (MQ=255)                                                         gCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACAttttttt                                                                                 >  2:205292/1‑148 (MQ=255)                                                           tttttGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCTCTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAg                                                                               >  1:42378/1‑148 (MQ=255)                                                                      tAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCat                                                                    <  1:211408/148‑1 (MQ=255)                                                                           aCTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCAtaataa                                                               >  1:58881/1‑148 (MQ=255)                                                                                           aTTTTTCTGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTATACTCCCGTCATAATAACTTCAGACAAACGTTa                                               <  1:95181/148‑1 (MQ=255)                                                                                                   gggTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGAcc                                       <  1:279418/148‑1 (MQ=255)                                                                                                     gTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTg                                     <  1:189512/148‑1 (MQ=255)                                                                                                     gTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTg                                     <  2:232309/148‑1 (MQ=255)                                                                                                                 tGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTaa                         >  2:274495/1‑148 (MQ=255)                                                                                                                           aTTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGt               <  1:76467/148‑1 (MQ=255)                                                                                                                            tttCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTTTGACCTGATAAATATTTAATTTTAAACGtt              >  2:210575/1‑148 (MQ=255)                                                                                                                            tttCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGtt              >  1:158721/1‑148 (MQ=255)                                                                                                                                       tCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGtctctct   >  2:70248/1‑148 (MQ=255)                                                                                                                                        ccAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGtctctctc  <  2:42378/148‑1 (MQ=255)                                                                                                                                        ccAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTACCGTCCTAATAAATTCAGACACTCGTTAGTATGACCTGATAAATATTTAATTTTACACGTTCTTGtctctctc  >  1:275021/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                             GAACAACATCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTC  >  CP000730/2642447‑2642729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑