Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	698,981	G→T	M46I (ATG→ATT)	mnhC1 →	CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	698,981	0	G	T	100.0%	35.9 / NA	12	M46I (ATG→ATT)	mnhC1	CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhC subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/6);  total (6/6)

GAATTTAATATTATTACTAGTTATAGGATTTTTAGTGTTTATAGGAACATATATGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATGGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGTTTAT  >  CP000730/698846‑699122                                                                                                                                        |                                                                                                                                              gAATTTAATATTATTACTAGTTATAGGATTTTTAGTGTTTATAGGAACATATATGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGt                                                                                                                                   <  2:295600/148‑1 (MQ=255)                                        tataGGAACATATATGATTTTATCAATCAATTTAATTAGTATAGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCaa                                                                                            >  1:8314/1‑148 (MQ=255)                                            ggAACATATATGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAAttgt                                                                                        <  1:106562/148‑1 (MQ=255)                                              aaCATATATGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAAttgttt                                                                                      <  1:36912/148‑1 (MQ=255)                                                     atGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATc                                                                               >  1:268115/1‑148 (MQ=255)                                                        aTTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCt                                                                            >  2:79797/1‑148 (MQ=255)                                                                              gTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCACTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAAc                                                      >  1:108650/1‑148 (MQ=255)                                                                                                      ataCTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGAt                              >  2:144605/1‑148 (MQ=255)                                                                                                            aTGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGc                        >  2:4834/1‑148 (MQ=255)                                                                                                               cTGGTAATCTCATTATTAGGATTGTTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGtt                     <  1:139839/148‑1 (MQ=255)                                                                                                                               tatGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGtt     <  1:247372/148‑1 (MQ=255)                                                                                                                                  gAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGTTtat  <  1:171622/148‑1 (MQ=255)                                                                                                                                        |                                                                                                                                              GAATTTAATATTATTACTAGTTATAGGATTTTTAGTGTTTATAGGAACATATATGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATGGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGTTTAT  >  CP000730/698846‑699122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑