Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,313,750	Δ1 bp	8.5%	intergenic (‑87/‑270)	yciC ← / → ompW	UPF0259 family inner membrane protein/outer membrane protein W

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	1,313,750	0	G→.	8.5%	7.3 ‑0.5 ‑0.0	47	intergenic (‑87/‑270)	yciC/ompW	UPF0259 family inner membrane protein/outer membrane protein W
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (16/27):  total (18/29)
Fisher's exact test strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.84e-01

CATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGTAGGATCATTTGTTACTCCAATGTAGGTATATTCG  >  NC_000913/1313454‑1313986                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               cATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGaaa                                                                                                                                                                                                                                            >  2:211138/1‑301 (MQ=255) acatttCTTACCCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGAGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGaaa                                                                                                                                                                                                                                            <  1:974509/297‑1 (MQ=255)      tCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCat                                                                                                                                                                                                                                       <  2:486588/301‑1 (MQ=255)         aaaCCAGGACGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCAATGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATc                                                                                                                                                                                                                                    <  1:407011/300‑1 (MQ=255)           aCCATGTCGAACAACCCACTACGGACGCTAACGGGCACGCCGGCATTGAGCTGCGCAAGCTGTGAATCACTTGGTTAGAAAACATGCCCTAACACCACTGTGATAAACGGACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCtt                                                                                                                                                                                                                                  <  1:636570/301‑1 (MQ=255)                      caacCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCggg                                                                                                                                                                                                                       >  2:630900/1‑300 (MQ=255)                          ccatgacgcccGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTa                                                                                                                                                                                                                   <  1:896493/292‑1 (MQ=255)                               cctGCCCCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGTTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGc                                                                                                                                                                                                              <  1:94001/300‑1 (MQ=255)                                             gCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCTCTGGGTGAGAAAACATGCCCTAACGCCTCTGTGATAAACGCACAAAGCAACGATACCAACAAAATTGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGTACTGCGCCGTGAGAGACATGCACTCTCCTGGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGTTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCa                                                                                                                                                                                                <  2:981157/300‑1 (MQ=255)                                                  ccGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGc                                                                                                                                                                                           <  2:636531/300‑1 (MQ=255)                                                     tCATTGAGCTGCGCAAGCTGTGCATCTCTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCCGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGTACTTGTATGGAAAAGCATATCTTTGTAAAGAGGAGGTAATTGCT‑GGTCTTCAGGCATGGGAaaaa                                                                                                                                                                                       >  1:170170/1‑300 (MQ=255)                                                     tCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGCTTCAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGCGCTAATTGCT‑TGCCTTCAGGCATGGCCAAAt                                                                                                                                                                                       >  1:714616/1‑301 (MQ=255)                                                                               aCTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAata                                                                                                                                                            <  2:934697/301‑1 (MQ=255)                                                                               aCTGGGGGAGAAAACATGCCCTAAAACCACTGTGATAAACGAACATAGCAAAGATACCAACAGAATTGTCAGAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCATTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAata                                                                                                                                                            <  1:630886/301‑1 (MQ=255)                                                                                cTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAatat                                                                                                                                                           <  2:924690/301‑1 (MQ=255)                                                                                      gagaAAACATGCCCTAACACCACTGTGATAAACGCACATAGTAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCg                                                                                                                                                      <  1:157381/300‑1 (MQ=255)                                                                                           aaCATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTAT‑GAAAAGTATAATTTTTGAAAGCGGGGGTAATTGGTGGGCCTCCGGGCAGGGGCAAATCG‑GGCAAGTGGGGGACGGAGTAAAATTTTGGGTAGGGa                                                                                                                                                >  2:891319/1‑301 (MQ=255)                                                                                                 cccTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAatat                                                                                                                                          >  2:767120/1‑301 (MQ=255)                                                                                                         ccacTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTaaaaaa                                                                                                                                  >  1:81615/1‑300 (MQ=255)                                                                                                         ccacTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAAt                                                                                                                                  >  2:838607/1‑301 (MQ=255)                                                                                                                                  cGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGAt                                                                                                         <  2:714642/301‑1 (MQ=255)                                                                                                                                  cGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGAATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGAt                                                                                                         <  2:81615/301‑1 (MQ=255)                                                                                                                                   gATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGAtt                                                                                                        >  2:241652/1‑301 (MQ=255)                                                                                                                                    aTACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGTTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATAtt                                                                                                                                                          <  1:880838/250‑1 (MQ=255)                                                                                                                                    aTACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAATT‑GGCGACCGAGTTAATAtt                                                                                                                                                          >  2:880875/1‑250 (MQ=255)                                                                                                                                    aTACCAACAGAATGGTCATAAAATGATTACGGAAGAAATTGCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTCTGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑TGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGACAATATTTAAAAATTGATTTAAATCACATTAACCAGGAt                                                                                                         <  1:854034/299‑1 (MQ=255)                                                                                                                                     tACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCAGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGACAAGCATATCTTTGCAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAAT‑GGCCACCGAGTTAATATTTGCGTTGGGAAAATTATTAAAAATCGATTTAAATCACATTTACACGGAtt                                                                                                        >  1:393926/1‑299 (MQ=255)                                                                                                                                                                        aaTTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAg                                                                   <  1:241647/301‑1 (MQ=255)                                                                                                                                                                          tttCCGGGTTCACTGTATACGGACTGCGCCGTGTAAGTAATGTACTCTCCTTGAGTGTTAGGCAGGTGTTAATTAGGGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAggg                                                                 <  1:767089/301‑1 (MQ=255)                                                                                                                                                                                        gTATACGGACTGGGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATAtt                                                                                                                                                          <  1:540760/198‑1 (MQ=255)                                                                                                                                                                                        gTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATAtt                                                                                                                                                          >  2:540771/1‑198 (MQ=255)                                                                                                                                                                                        gTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCatatt                                                   >  1:302758/1‑300 (MQ=255)                                                                                                                                                                                        gTATACGGACTGCGCCGTGATAGACATGCACTCCCCTTGAGTTTTATGCAGGTGTTAATTATCGGGCAATGGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTGTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCAAGGGCAAATCGCGGAAAGT‑GGCGACCGAGTTAATATTAGCGGAGCGAAAATATTTAAAAATTGATTTAAATCGCATTAACCAGGATTCACAATGCAACTTCTAAATTAATCCAGAACAATAACGGGTACATTAACaaata                                                   >  1:193927/1‑300 (MQ=255)                                                                                                                                                                                                           aTAGACATGCACTCTCCGTGAGTTTTATGCAGGTGTTAATTATCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑TGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GTCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGTAGGa                                <  2:170172/301‑1 (MQ=255)                                                                                                                                                                                                            tAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGTAGGAt                               <  1:564521/301‑1 (MQ=255)                                                                                                                                                                                                            aaGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGTAGGAt                               <  2:302764/300‑1 (MQ=255)                                                                                                                                                                                                            aaGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGTAGGAt                               <  1:838574/300‑1 (MQ=255)                                                                                                                                                                                                                      ctctCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTAAATGCAACTTCTAAATTAATCTAGATCAATAAAGGGTGAATTATCATATGTAATGTGATATATGTAGGATCATTTGTTa                      >  1:281712/1‑300 (MQ=255)                                                                                                                                                                                                                      ctctCCTTGAGGTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGTAGGATCATTTGTTAc                     <  1:211133/301‑1 (MQ=255)                                                                                                                                                                                                                        ctcCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAggg                                                                 <  2:193884/255‑1 (MQ=255)                                                                                                                                                                                                                        ctcCTTGAGTTTTATGCAGGTGTTAACTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAggg                                                                 >  1:193880/1‑255 (MQ=255)                                                                                                                                                                                                                              gatttttATGCAGGTGTTAATTGCCGTGCAAGTTTACCCTGGTAAGGCTTCGGGTGGAAGAATCGGGTTTTGAAT‑GAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGTAGGATCATTTGTTACTCCAATGt             <  1:891282/297‑1 (MQ=255)                                                                                                                                                                                                                                           gtgtTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTACCATATGTAATGTGATCTATGTAGGATCATTTGTTACTCCAATGTAGGTATATTCg  <  2:193931/299‑1 (MQ=255)                                                                                                                                                                                                                                           gtgtTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTAT‑GAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGt                                    >  2:380811/1‑264 (MQ=255)                                                                                                                                                                                                                                           gtgtTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTAT‑GAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGt                                    <  1:380804/264‑1 (MQ=255)                                                                                                                                                                                                                                                                      ggTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGt                                    <  2:265405/238‑1 (MQ=255)                                                                                                                                                                                                                                                                      ggTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGt                                    >  1:265400/1‑238 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               CATATTCTGAACCAGGTCGAACAACCCACTACTGCCGCTAACGGGCACGCCGTCATTGAGCTGCGCAAGCTGTGCATCACTGGGTGAGAAAACATGCCCTAACACCACTGTGATAAACGCACATAGCAACGATACCAACAGAATGGTCATAAATTGATTACGGAAGAAATTTCCGGTGTCACGGTATACGGACTGCGCCGTGATAGACATGCACTCTCCTTGAGTTTTATGCAGGTGTTAATTAGCGGGCAATTGTACCCTGGTTAAGCCTCGGGTGGCAGCATCAGGTCTTGTATGGAAAAGCATATCTTTGTAAAGCGGAGGTAATTGCT‑GGCCTTCAGGCATGGGCAAATCGCGGCAAGT‑GGCGACCGAGTTAATATTTGCGTAGCGAAAATATTTAAAAATTGATTTAAATCACATTAACCAGGATTCTCAATGCAACTTCTAAATTAATCCAGATCAATAAAGGGTGAATTATCATATGTAATGTGATCTATGTAGGATCATTTGTTACTCCAATGTAGGTATATTCG  >  NC_000913/1313454‑1313986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.