Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC NC_000913 1,907,562 IS1 (+) +9 bp 45.1% coding (22‑30/144 nt) yobF ← DUF2527 family heat‑induced protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)47 (0.410) 38/550 NT 43.5% noncoding (768/768 nt) IS1 repeat region
?NC_000913 1907562 = 59 (0.530)coding (30/144 nt) yobF DUF2527 family heat‑induced protein
* ? NC_000913 = 190757059 (0.530)50 (0.430) 39/550 NT 45.0% coding (22/144 nt) yobF DUF2527 family heat‑induced protein
?NC_000913 = 1979270 NA (NA)noncoding (1/768 nt) IS1 repeat region

AACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGACAGCAGGCTCATAAGACGCCCCAGCGTCGCCATAGTGC  >  NC_000913/257619‑258194
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                              
aaCGATCCGTTCAGACCGGAATAGGACTCACGCTTAATCACCTCATCATCTGCTGAAGGTAAATTATGAGGGAGCCCACTTAACCCGGTGGATCGGAATTTTGACAAAGGGCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAAGTTGCTAAATTGTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAaggtaagtgcaaagat                                                                                                                                                                                                                                                                                      <  1:764613/300‑9 (MQ=255)
              cccGGAATAGGACTCACGATTAATCACCTCGTCATCTTGTGACGGTGAATTATTGTAGATCCCATTTAACCCGGTGTATCGGAATTTTTACAAAGGTCTTAAGGAAATCACTGACTGGATCGAAAAACTCGTGTGGGAAAAATGTGCTAAATTTTGCCAATTTGGTACAACAGTGGCATCACGACAGTAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGGAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAaggtaagtgcaaagataatcgattcttttt                                                                                                                                                                                                                                                                        <  1:709664/300‑23 (MQ=255)
                                                                                                                                                                                                                                                                                   aaataaacatggtcggtaATGACTCCAACTTACTGATAGTGTTTTATGTTCAGATAATGCCCGATGACCTTGTCATGCAACTCCACCGATTTTGAGAACGACAGTAACTTCCGTCCCAGCCTTGCCAGATGTTGTCTCAGATTCAGATCATGTCGCTCAATGCGCTGAGTGTAACGCTTTCTGATAACGTCCAGCTTTCCCTTCAGGCGCGATTCATACAGCGGTCAGCCATCCGTCATCTATACCACGACCTCAAAGGCCGACAGCCGGATACAAATAAGCCACTGCGTGGCCAGAGTgg  >  1:94106/11‑300 (MQ=18)
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                              
AACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGACAGCAGGCTCATAAGACGCCCCAGCGTCGCCATAGTGC  >  NC_000913/257619‑258194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.