Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	15,660	0	A→T	100.0%	1.3	3	L72F (TTA→TTT)	insL1	IS186 transposase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/0):  ref base (0/0):  total (3/0)

CCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAA‑GTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGACGTGGCTCTCCTGAAGCGG  >  NC_000913/15365‑15684                                                                                                                                                                                                                                                                                                         |                         ccTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACCCCTGATATGATTTAACGTGCCGCTGAATTACTCTCACGATAACTGGTCAGCCATTCTGGCCCATCTTGGTAAGCCCGGAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTCCGTCTGTGGCTGGATTCCGGCCCCGGGGGGATGTACTTAAGAGAA‑GTTACAGCATGGGGTACGCTACCAGACGTTGCACAATTTTCTg                      >  1:204091/1‑300 (MQ=11)            gCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTCACCCGCCGCCGCGAACTTCGTGATGCTGCAACTCTGCTACGTCTGGGGCCGGCTTCCGGCCCCGGGGGGATGTCATCACGTGAA‑GCTCCTGCATGGGCTCATCTCCCTCATCTTGCAACCTTTTCTGACGTGGCtctc           >  2:525940/1‑300 (MQ=11)                      cTCCCATAAGCGCTAACTTAAGGGTTGTGGGATTACGCCTGAGATGATCTAACGTGCCGATGACTTACTCTCACGATAACTAGTCATCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGCCAGTTCGGCACGTAATGCCGGCGCTCTAACCCGCCGCCGCGAAATCCGTGACGCTGCAACTCTCGTACGGCGCGGGCTGGCTTACGGCCCCGGGGGGCTGTCCGTCCGTCAATATCA‑TGCATCGCCTCCGCCACATGACGTACCAACATTCTCTGACCTGCCTCTCCtaccacgc  >  2:51744/1‑292 (MQ=11)                                                                                                                                                                                                                                                                                                         |                         CCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAA‑GTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGACGTGGCTCTCCTGAAGCGG  >  NC_000913/15365‑15684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.