Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,997,418	0	G→C	100.0%	1.4	3	V61V (GTC→GTG)	insC1	IS2 repressor TnpA
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/2):  ref base (0/1):  total (0/3)

GAACAACCTGTTCTCCGGCGGCGACAGCAGTAAGACTTCCTTCCTGGTATTGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGG  >  NC_000913/2997396‑2997711                       |                                                                                                                                                                                                                                                                                                      gtaccaccttggtgccGGCCGCCGCAGCAGTAAGACGTCCTTCCTGGTATTGCTTACGCCAGCGAAGTAACTGGCTGGCTGCTACACCATGTTGCCTCGCAACGAGGGAGACCGTCAGCCCCGGTGCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCGTAAGACAGCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACt                  <  2:522735/286‑1 (MQ=11) gAAAAACCTGTGCTCCGGTGGCGAAGGTCGTAAGACGTCCTTCCGTGTATTGGTGATGCAACAGAAATAACGGGCTGGCTGCTATACCATGTTGCCGGGCAACGAGGGAGACCTTCATCCCGCGTTCAAAGCTCTGCTGAACAATTTCTGTCTTTTCCTCTGTGGTACGCCGTCTGCGTTTCTCCTGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATGGGTTGTCTGGAGATTCAGGGGTCCAGTCTAAACt                  <  2:300718/300‑1 (MQ=11)                cggcggCCACAGCATTAATACTTCCTTACTGGGATTGCTTACCCCAGAGAAATAACTGGCTGTCTGCTACACCAGGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTgg  <  2:620232/301‑1 (MQ=255)                       |                                                                                                                                                                                                                                                                                                      GAACAACCTGTTCTCCGGCGGCGACAGCAGTAAGACTTCCTTCCTGGTATTGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGG  >  NC_000913/2997396‑2997711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.