| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,966,582 | C→T | 80.4% | G618D (GGC→GAC) | ptsP ← | PEP‑protein phosphotransferase enzyme I; GAF domain containing protein |
| Read alignment evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | change | freq | score | reads | annotation | genes | product | ||
| * | NC_000913 | 2,966,582 | 0 | C→T | 80.4% | 37.5 ‑0.4 ‑1.0 | 117 | G618D (GGC→GAC) | ptsP | PEP‑protein phosphotransferase enzyme I; GAF domain containing protein |
| Reads supporting (aligned to +/- strand): new base (55/35): ref base (13/11): total (68/49) | ||||||||||
| Fisher's exact test strand distribution p-value = 6.41e-01 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.09e-01 | ||||||||||
Only 100 of 117 total aligned reads displayed.
GCTGCGCAAGATTTTCTGCTTCGGCATAATCAATGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACC > NC_000913/2966286‑2966874 | ccggcgcAGAATTTCCTGCTGCGGCATAATCAATGGGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGGCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAAc < 2:630867/298‑1 (MQ=255)ccggcgcAAGATTTTCTGCTTCGGCCTAATCAATGCGCCGCAGCAGGTAATTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCAAA‑TGGGATCGCCCGCCAGTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAAc < 1:918661/298‑1 (MQ=255) gcgcAAGATTTTCTGCTTCGGCATAATCAATGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCAACCCGGGTATTTTTGCGATCAACGGCCCGAATGTATTGAGTCAGATCGTTGGTGTCAACaga > 1:927575/1‑301 (MQ=255) aaGATTTTCTGCTTCGGCATAATCAAGGCGCCGAAGCAGTGATTTTGCCCGCGCACCAGAACGCCCGTTCATAGCCCGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATg < 2:57716/301‑1 (MQ=255) aTTTTCTGCTTCGGCATAATCAATGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGCGTACGATCGTTGGTGTCAACAGACATGaaa > 1:514630/1‑301 (MQ=255) cTTCGGCATAATCAATGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGAccc > 1:653494/1‑300 (MQ=255) aaTCAATGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACAGCACACACGGAGATCGATTCAATGTATTTCAGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATTAAGACTGTAATACATGTTCGCCAACCGGTTATTGTTGCGATCACCGGCCAGAATGTATTGAGTCAGATCGTTAGTGTACACACATATGAAATCGACCCGATTTGCCAg > 1:251349/1‑301 (MQ=255) tCAATGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAGTGTATTAAGTCCGATCGTTGGTGTCAAAAGAGATGAAACGTACCCGCTTTGCCacaa > 2:634053/1‑298 (MQ=255) gcagGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACC‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATAGTTGGTGTCAACTGAGATCAAATCGACCAGCTTTGCCAGATG‑CGG‑CCGCATaaa > 1:629530/1‑301 (MQ=255) gtatggctcccGCGCCACACAACGTCCGCTCAAAGACAGATGGCGAGCCCCAAGCCCAATGAGGATTGCAACG‑ACACGGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGAGCCACGTCCAGAATGTAGTGAGTCAGAGCGTTGGTGCGAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CATCATAAATAc < 2:91907/292‑1 (MQ=255) gTATTTTGCCCGCGCCACAAAACGTCCGTTCATAGTCAGAGGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATAc < 1:376359/300‑1 (MQ=255) gTATTTGGCCAGCGCTACAGAACGTCCGTGGAGAGAAAGAGGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTGACCGCACAAACGGAGATCGATTCCATGTATTGCCGCTTCCAGGGCGATCAGTGCCAGAGCTCGTAACATTGCAGGATGAAGAGTGGCATAAATGGTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGTCAACAGAGAGGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATAc < 2:712184/300‑1 (MQ=255) gTATTGTGCCCGAGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATAc < 1:676137/300‑1 (MQ=255) tttGCCCGCGCCAAACAACGTCCGTGAAGAGACAGATGGCAGAACCCAAGACAAAGGAGGTGTACCACGCACG‑TTGGAGCGCCCGCCCTTTACCCGCACAGACGGAGAGCGATTCCATGTATTTCCGCGTACCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGGCATAAATGTTCGCCACGCGGGTATTGTTGCGATCAACGGCCCGAATGTAGTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTg < 2:322601/301‑1 (MQ=255) cctggtcttaaaaaCGTCCGGTCATAACCCGATGGGGATACCCAAGACCAAGGAGGATTGCCACGCACC‑TGGGACCGACCGCCATTTCACCGCACAAACGGCGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATTAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACgg < 2:436170/289‑1 (MQ=255) cTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGCGCCCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCCCCCGGGTATTGTTGCGCTCAACGGCCAGAATGTTTTGAGTCAGATCGTTGGTGTCAACCGAGCTGAAGTCAACCCGCTTTGCCAGATT‑CGG‑CAGCATAACACCCATTGAGGGCAAtt > 1:897143/1‑300 (MQ=255) cTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCCTAAAAACCATTGACGACACtc > 2:664560/1‑299 (MQ=255) aaCGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAg < 1:664583/298‑1 (MQ=255) aaCGTCCGTTCAAAGACAGATGGCGATCACCAAGCACAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGCTCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCat < 1:335983/301‑1 (MQ=255) tCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATACATGTTAGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCATCAGTGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTTACGGCACTTCCACCatg > 1:76986/1‑298 (MQ=255) ccGTTCAGATACAGATGGCGATAACCAACCCCAATGAGGGTGTCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGGGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGGAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGc < 2:930755/301‑1 (MQ=255) ttCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCACCGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATACATACCATTGACGGCACTTCCAGCCTGATGCCg > 1:690889/1‑300 (MQ=255) ttCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCCGCATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGCCCCGCTTTGCCAGATG‑CGG‑CAGCATACATACCATTGACGGAACTTCCAGCATGATGCCGa > 1:268415/1‑301 (MQ=255) ttCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATAGCCAGAGCTCGTAACATTGCAGGATGACGACTGTCATAAATGTTCACCAACCAGGTATTGTTGCGATCAACGGCCCAAATGTATTGAGTCAGATCGTTGGGGTCAACAGAGATGAAAGCGACACGCTTTGCCAGGTG‑CGT‑CAGCATAAATCCCATTGACGGAACTTCACGCATGATGCCgg > 1:728963/1‑300 (MQ=255) ttCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGCGCGATCATCGCCAGAGCTCGTAACATTGCAGGCTGAAGACTGTCATAAATGTTCGCCACCCGGGCATTGTTGCGATCAAAGGCCATAATGTATTGAGTCAGCTCGCTGGTGTCCTCAGGGATGAAATCGACCCGCTTTGCCCGGTG‑CGG‑CAACCTAAACACAATTGACGGCCCTCCCCGCACGCTGCCGa > 2:150202/1‑301 (MQ=255) ttCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGAGCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAACTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTAGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAGATCGCCCCGCTTTGCCAGATG‑CGG‑CAGCATAACTACCATTACCGGCACTTCCAGCATGCTGACGa > 1:615733/1‑301 (MQ=255) cAGATGGCTATACCCAAGCCCACTGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGCTCGATTCCATTTATTTCCGCTTCCCGGGCGATCATCGCCATAGCTCGTAACATTGCCGGATGAAGACTGTCATCAATGTTCCCCCCCCTGGTCTTCTTGCGCTCAACGGCCCTCATGTATTTCGTCAGATCCTTGGTGTCAACCTAGATGACATCGACCCGCTTTGCCAGCTG‑CTC‑CCGTATAAATCCAATTGCCCTCACTCCCaccctcctgcccgcaccgcgt > 2:423806/1‑281 (MQ=255) aTGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATGTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTgg < 1:267347/297‑1 (MQ=255) gCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCTGGCGATCATCGCCAGAGCTCGTAAAATTGCCGGATGAAGACTGTCATAAATGTTCGCCACACGGGTATTGTTGCGCTCCACGGCCAGACTGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTTCCAGATG‑CGG‑CAGCATAACTCCCATTTCCGGCACTTCCGGCATGCTGCCGAGCCGGTGTTTggag > 2:62374/1‑299 (MQ=255) gCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGCATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATT‑CGG‑CAGCATAACTACCATTGACGGCACTTCACGCATGATGCCGAGCCTTGGCTTGGGa > 1:983572/1‑301 (MQ=255) cGATACCCAAGCCCAACGAGGAGTGCCAAGAAAC‑TGGGATCGGCCGCCACTTCACCGCACAAACGGAGAGCGATTCCATGTATTTCCGCTTCCCGGGCGATCAGCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCAGTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGaa < 2:580883/301‑1 (MQ=255) tACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGAGGAAGACTGTCATAAATGTTCGCCCCCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGGCAGATCGTTGGTGTCAACAGGAAGGAAATCGACCCGCTTTGCCAGCTG‑CGT‑CCGCAAAAATACCATTGCCGGCCCTTCCAGCATGATTCCGAAAGGTGGGTGGGGAAttt > 2:216016/1‑301 (MQ=255) aCCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTc < 1:812876/268‑1 (MQ=255) cccAAGCCCAATGAGGATTGCCACGCACA‑TTGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGCGTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGCAGACTGTCACAAATGTTCGCCCCCCGGGTATTGTTGCCATCAACGCCCAGTATGTATTTAGTCAGCTCGTTACGGCCAACAGAGATGAAATCGACCCGCTTTTACGTATC‑CGA‑GCTCATAAATCCCATTTACGGCACTCCCAGACTTATGCAGCTCCGTGGTTTCACAATTTca > 2:890581/1‑300 (MQ=255) aGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGCTG‑CGG‑CAGCCTAACTACCATTGACGGCCCTTCCAGCATGATTCCGATACGTGGTTTGGGAATTTCGTaa > 1:609190/1‑300 (MQ=255) ggATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTACCATTGCAGGATGACGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGCTCGTTGGTGTCAACAGAGATGAAATCGACTCGCTTTGCCAGATT‑CGG‑CAGCATAAATACCATTTACGCCACTTCCAGCCTGCTTCCGCTCCGTTGCTTGGGAATTTCGTACCCGATCTTCCCCTCGCCTTCCCGCCCgg > 2:314734/1‑301 (MQ=255) tCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGACGCCTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGCATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGAGG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGGTCCGTGGTTTTGGAATTTCGTCACCGCTCATCTCCTCCCCTTCCCGTCCGGCACTTTCCATCCgg > 2:552355/1‑300 (MQ=255) cacaAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAACTGTTCGCCACCCGGGGATTGTTGCGATCAACGCACAGACTGTATTGAGTCACATCGTTGGTGTCCACAGAGATGCAATCGACCCGCTTTGCCAGATG‑CGC‑GATCATAAATACCATCGACGTCACTCCCAGCTTGATCCCGATACGTGGTTTTGGAAGTTCGTAACCCATCATCTCCTCGCCTTCACTTACGGCACTTTCAATCATGTCGCGTg > 2:546035/1‑301 (MQ=255) aaCGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTCCGTAACCGATCATCCCCTCGACTTCACGTCCGGCACGTTCCACCAGGCGGCGTGCtt > 1:131894/1‑300 (MQ=255) gaTCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATCAATGTTCGCCACCCGGGTATTGTTGCGATCACCGGCCAGAATGGATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGCCGTCACTTCCCGCATGATGCAGATACGTGGTTTGGGAATATCGTCACAGATCATCCACTCGACTTCCCGTCCGGCACGTTAAATCAGACGCCGTCATTCATCaaa > 2:91033/1‑300 (MQ=255) aTTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCCCGTCCGGCACGTTCAATCCGGCGGCGTGCTTCATCAACTTCAt > 1:325451/1‑301 (MQ=255) ccATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGCTCAACGGCCAGAATGTATTGAGTCAGATCGCTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCCGATT‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCCTGATTCCGATACGTGGTTTGGGAATTTCGTAACCGATACTCTCCTCCACTTCACGTCCGGCCCGTTCAATCCGGCGCCGGTCTtcataacattccccag > 2:690374/1‑289 (MQ=255) aTGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGCTGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCCCGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAgg > 1:276781/1‑301 (MQ=255) aTGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAgg > 1:758038/1‑301 (MQ=255) ggagtcccGCTTCCAGGGCGACCAACGCCAGAGCCTGTAACATTGCAGGATGAAGACGGTAATAAATGTTCGCCGCCCGGGTATGGTTTCGATCAACGGCCAGAAGGCATTGATGCAGATCGTTGGTGTCAACATAGATGAAATCGACCCGCTTGGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTGCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGc < 1:947775/294‑1 (MQ=255) gTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAACTCGACCCGCTTTGCCCGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATAAGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCACTCAGACGGCGTGCTTCATCAACTTCATCGCgg > 1:768068/1‑299 (MQ=255) ccGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCAGCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGAc < 1:435791/301‑1 (MQ=255) ccGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGAc < 1:634075/301‑1 (MQ=255) cgtttccctgccactctatccctgtgCTCGTAACATTGACGGATGAAGCCGGTCATAAAGTGTCTCCACCCGGGTATTGGTGCGAGCACCGGCCCCAATGCATTGAGTCAGATCGTGGGTGCCAACAGAGATGAAATCTCCCCTCTTTGCCCGATG‑CGG‑CAGCATAAATACCATTGACCGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGAcc < 1:71991/276‑1 (MQ=255) cTTCCCGGGCGATCATCGCCAGAGCACGTAACATTGCAGGATGAAGACTGTCATACATGTTCGCCACACGGGTATTGTTTCGTTCAACGGGCAGAATGCATTGAGTCAGATCCTTGGTGTCAACAGAGCTGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCCTTGACGGCACTTCCCTCATGCTGCCGCTCCGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCCCGTCCGCCGCGTTCACTCCGCCGCCGTGCTTCCTCCCCTTccccgtgcctgttgccac > 1:308361/1‑283 (MQ=255) gataacctcaagggctcggaaaggtGCAGGAGGAAGACTCTCAGAAATGTTCGCCACCCGGGTATTGTGTAGATCAACGGCCAGAATGTATTGAGTTAGATCGGTGGTGCCAACAGAGATTAACTCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACGTCCAGCATGATGCCGATACGTGGTCTGGGAATTTCGTAACCGACCATCTCCTCGACGTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATAAACTTCATCGAGGCTTGTGACCATCGGCAACAg < 2:681157/276‑1 (MQ=255) atcatcGCCGAGGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTTAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatc < 2:884795/216‑1 (MQ=255) atcatcGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCCTGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCCCGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGaa > 1:155404/1‑301 (MQ=255) atcatcGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑AAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatc > 1:884822/1‑216 (MQ=255) tcGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGGATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCCACAGAGATGAAATCGACCCGCTTTTCCAGATG‑CGG‑CAGCATAACTACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGCCTTCACGTCGGGCACGTTCAATCAGGCGGCGTGCTTCCTCACCTTAATCCAGTCTTGTGACCATCGGCCAccaaatctt > 2:737156/1‑293 (MQ=255) cGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAAGGTGCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTc < 1:787112/301‑1 (MQ=255) gcaagagCTCGTAACATTGCAGGATGAAGACTGTAATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTGGGTGTCAACAGAGATGAAACCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTc < 2:653471/297‑1 (MQ=255) agCTCGTAACAGTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAgg < 1:168603/298‑1 (MQ=255) agCCCGGAACATTGAAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATGGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAGTTCGGAACCGATCAGCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAgg < 1:94179/298‑1 (MQ=255) gCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGAACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGGCCGGACCGTTCAATCAGGGGGCGTGCTTCATCAACTTAATCGAGGCTTGTGACCATCGGCAACAGACTATTCCGGTTg > 2:126061/1‑300 (MQ=255) gCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACTATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGACTTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTTCTTCATCAACTTAATCTAGGCTTGTGCCCATCGGCAACAGATTCTTCAGGTTg > 1:272269/1‑300 (MQ=255) gCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGCTACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCCGCGTGCTTCCTCAACTTCCTCGAGGCTTCTGACCATCCGCAACCGAATTTTCAGGTTg > 1:207688/1‑300 (MQ=255) ttgTAAAAGTGCAGGATGAAGACTGTCAGAAAGGTTCGCCACCCGGGTAGTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGGCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGccc < 1:62375/299‑1 (MQ=255) ggATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCCGGTTTCCCGTAGCGGCATTa > 1:350578/1‑300 (MQ=255) ggATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTACAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAAACGATCACCTCCTCGACTTCACGTCCGGCAGGTTCACTCGGGCTGCCTCCTCCATCAAATTCAACGTGGCTTTTGACCCTCGGCAACATAATCTTCATGTTCCCCTTACCGGcctga > 2:225094/1‑296 (MQ=255) gctGACGACTGTCATAAATGTTCGCCACCCGGGTATTGTCGCGATCAACGGCCAGAATGTATTGAGTAAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CACCATAAATACCATTGACGGCACTTCCAGCATGATTCCGATACGTGATTTTGGCATTTCGTAACCGACAGACGCCTCTACTTAACGTACGGCACGGCCCATCATGCGGCGTGCTTTATAAACTTCATCGAGCCACTTGCCCTTCGGACACAGACTATTCAGTTTACCCGTAACGTCAtcac > 2:228766/3‑297 (MQ=255) gATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCCCGTTCAATCAGGCGGCGTTCTTCCTCAACTTCATCGAGGCTTGTGACCATCGTCAACAGAATATTCAGGTTGCCCTTAGCGGCATTAg > 2:269213/1‑300 (MQ=255) gagttagaaaaaggttgccagcgagtgaTTGTTGAGATCATAGAGCAACATGTATGCGGTCAGATTGTTGGTGTCAACAGAGATGAAAGCGACGCGCATTGCCAGGTG‑CGG‑CAGCATAAATACCATTGACGGCACGTCCAGCAGGATGCCGATACGTGGTTTTGGAATTTCGTAACCGATCATCTCCTCGAGTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCAGCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcag < 2:23900/273‑1 (MQ=255) tGTTATAAATGTTCACCACCCGGGTATGGTTCGGAGAAACGGCCAGAATGTATTGAGTCAGATCGTTGGTACCAACAGAGATGAAATCGACCCGCTTTGCCAGCTG‑CGG‑CAGCATAAAGACCTTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCAt < 2:268320/301‑1 (MQ=255) tGTCAAAAATGTTGGCCACCCGGGTATTGTGGCGATCAACGGCCAGAATTTATTGAGTCAGATCGTGGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCGTGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCGCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGTCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACTCAGCAt < 2:76984/301‑1 (MQ=255) gTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGCTGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTACAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGCTCATCTCCTCGACTTCACGTCAGGCAAGGTCAATAAGGCGTAGTGCTTCGTCGACTTCATCGAGGCTTGTAACACTAGGCAACAGACTATTCAGGTTGCCCATAGCGGCATTAGCACgcagccta > 1:816602/1‑298 (MQ=255) gTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCTTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAAAAGACTATTCAGGTTGCCCTTGGCGGCTTTAGCATgccgcaa > 2:706552/1‑299 (MQ=255) gTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCCTCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcagcaca > 2:357806/1‑299 (MQ=255) gTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCCTAACCGATCATCTCCTCGACTTCACGTTCGGCACGTTCACTCAGGCGGCGTGCGTCATCAACTTCCTCGAGGCTTGTTGCCATCGGCAACAGAACATTCGGGTTGCCCGtctagtcatcacccacaaccatt > 2:168120/1‑279 (MQ=255) ccACCCGGGTAGTGTTGCGATCAACGGCAAGAATGGATTGAGTCAGATCGTTGGGGTAAACAGAGATGAAATCGAACCGCTCGGCCAGATG‑CGG‑AAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTGTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGAt < 2:514621/301‑1 (MQ=255) ccACCAGGGTATTGTTTGTATCACAGGCCAGAATGTATGGAGGCAGGTCGTGTGTGTCAACAGAGATGAAATCGACCAGCTTTGCCAGATG‑CGG‑CAGCATAAATACTATTGACGGAACTTCCAGCATGATGCCGATACGTGGTTTGGGAATGTCGTAACAGATAATATCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGAt < 1:423813/301‑1 (MQ=255) acaaggggGATTGTTGCGATCAACGGCAAGAATGTAGTGAGTGAGATCGTTGGTGAGAACAGAGAGGAAATCGACACGCTGGTCAAGAGT‑CGG‑CAGCATAAATGCCAGTTACGGAACTTCCAGCATGATGCCGTTACGTGGTTTGGGAATTTCTTAACCGAGCAGCTCCTCGACTTCACGGCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACAATCGGCAACATAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGAt < 2:251343/295‑1 (MQ=255) cccGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCCCACCTGGACCaa > 1:638831/1‑301 (MQ=255) gggttgtgttgCGAGAAACGGCCAGAATGTAGTGAGTCAGATCGGTGGTGTCAACAGAGATGAAATCGACCCGCGTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCAGGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCaaga < 1:867830/294‑1 (MQ=255) gTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCTGCCTTCGCACGCAGCATGCCCCGCCCCTGGATCaagaaaa > 2:238552/1‑299 (MQ=255) aTTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTATGGGAATTTCGTAACCGATCATCCCCTCGACTTCACGTCCGGCACGTTCAATCAAGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCAGCGGCAAAAGAATATTCAGGTTGCCCGGAGCGGCGTTGGCACGCAGCAGCGCCCACACCCGGATCaagagaat > 2:354752/1‑296 (MQ=255) ttgttgCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGAAGGCACTTCAAGCATGATGCCGATACGGGGTTTAGGACTTCAGTACCCGATCATCTCCTCGACTTCACGTCCGGCACGTTCCATCAGGCGGCGTGCTGCATCAACTTCATCGAGGCTTGTGACCATCGGCCACAGAATATTCAGGTTGCCAGTATCAGTCTTAGCACGCAGCATCGCCCGCACCCGGAGCAAGAACAtg > 1:413605/1‑299 (MQ=255) gttgCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCCTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAACGGCCTTTGGACGCAGCATCCCCTGCACCTgggcccagacagccgc > 2:197820/1‑286 (MQ=255) gttgCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCAGCTCCTCGACTTCACGTCCGGCCCGTTCAATCAGGCGGCGTGCTTCCTCAACTTCATCGAGGCTTGTGACCATCGGCAACACCATATTCCGGTTGCCCGTAAGGGCCTTATCACGCAGCCTCGCCCCCCCCTGGAACAACAAAAtctc > 2:25609/1‑300 (MQ=255) tCAACGGCAGGAATGCATTGAGTCAGTACGGGGGTGTCAACGCGAATGAAAGCAGCCCGCTTTGCCA‑ATGACGGACAG‑ATAAATACCATTGACGGCACTGCCAGCATGCTGCCGATACGTGGTTTGGAAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTGCATCGACTTCGTCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCAGCATTAGCACGGAGCATCGCCCGCACCTATGTCAAGAAGATCTCCGGCTGAt < 2:131891/301‑1 (MQ=255) ccAGAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCAGATCGAGCGt < 1:269219/301‑1 (MQ=255) gAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCCCTTCCAGCATGATGCCGCTACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTACTACCCAGCCTCGCCCGCACCTCGCTACAGCAGGTCTCCGGCTGATCGAGCGTAAt > 1:54624/1‑301 (MQ=255) gAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCCACTTCATCGCGGCTTGTTACCATCGGCAACAGAATCTTCAGGTTGCCCGTAGCGGCATTAGCCCGCCGCATCGCCCCCCCCTGGACCCACACGCTCCCCGGCTgcttcgccgtac > 2:699943/1‑289 (MQ=255) gAATGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATACAGAAGATCTCCGGCTGATCGAGCTTAAt > 1:483172/1‑301 (MQ=255) aaTGTTTGGAGTCAGATCGGTGGGGCCAAAAGAGAGGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATg < 2:264676/301‑1 (MQ=255) aaTGTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGCCTGATCGAGCGTAAtt > 1:819755/1‑300 (MQ=255) gTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatcatc > 1:337593/1‑133 (MQ=255) gTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatcatc < 2:337586/133‑1 (MQ=255) gTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGt < 2:468352/148‑1 (MQ=255) gTATTGAGTCAGATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGt > 1:468360/1‑148 (MQ=255) gTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCCAGAAGATCTCCGGCTGATCGAGCGTACTGCGa > 1:609448/1‑301 (MQ=255) tggAGTCAGATCGGTGGTGCCAACAGAGAGGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACGTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATc < 1:552365/299‑1 (MQ=255) aGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATAACATTGACGGCACTTCCAGCATGATGCCGATACGTGGGTTGGGAATTTCGTAACCGATCATCTACTCGACTTCACGTCCGGCACGATCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACGATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCACGCACCTGGATCACGAAGCTCACCGGCTGATCGAGCGTTATGCGAATccc > 1:381176/1‑300 (MQ=255) gATCGTTGGTGTCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTAAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCCGGTTACCCGTAGCGGCCTTAGCACGCAGCATCCACCGCCCCTGGATCCGGAGGATCTCCGGCTGATCCAGCGTAACGCCCATCCACCGCCa > 2:75547/1‑301 (MQ=255) cGTTGCGGCCAACAGAGACGAAATCGACCTGCTTTCCCAGTGT‑CGG‑CACATTAAATACCATTGACTGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGTTCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCTGCTTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAAcc < 1:21363/301‑1 (MQ=255) | GCTGCGCAAGATTTTCTGCTTCGGCATAATCAATGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACA‑TGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATG‑CGG‑CAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACC > NC_000913/2966286‑2966874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |