Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,296,191	A→C	33.4%	intergenic (+397/+245)	gltP → / ← yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	4,296,191	0	A→C	33.4%	22.0 ‑1.0 ‑0.3	50	intergenic (+397/+245)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  new base (12/5):  ref base (18/14):  total (31/19)
Fisher's exact test strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.08e-01

TAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACC  >  NC_000913/4295896‑4296482                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGt                                                                                                                                                                                                                                                                                                     >  1:214563/1‑301 (MQ=35)   gCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGt                                                                                                                                                                                                                                                                                                     >  1:213958/1‑299 (MQ=35)        aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCTACATCAACGCCTGATGCGCCGCTTAACGCGTCTTATCAGTCCTACTCCAGACAGCGCAATAGCCTGCTTTCTCGTGATTTTGTAGGTCGGATAAGTCGTTTACGCCTCATCCGACATCCACGCCTGATGCGACGCTTGCCGCGTCTTATCCGGCCTACGCCAGGCAACGCAATAGCCTGATTTATCGTGATTTTTTAGTTCGGGTACGGCGTTTCTGACGCATCCGCCAGCACTGCCTGGTGCT‑TGGCTTACCTCGTCTTCTCa                                                                                                                                                                                                                                                                                              >  1:790508/1‑301 (MQ=11)            tGTAGTCCGGATAAGGCGTTTATGCCGCATCCGCCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGcc                                                                                                                                                                                                                                                                                          <  1:300794/301‑1 (MQ=25)                  tCGGCTAAGGCGTTTATGCCGCATCCGACATCCACGCCTGATTCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGc                                                                                                                                                                                                                                                                                     <  1:840413/300‑1 (MQ=25)                     gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTCTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCCGATAAGTCGTTTATGCCGCCTCCCACACACACCCCTGATTCG‑ACGCTTACACCGTCTTATCAGCCCTCCGCCaca                                                                                                                                                                                                                                                                                 >  1:502641/1‑299 (MQ=11)                     gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                                                                                 >  1:419902/1‑301 (MQ=35)                     gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGAGTGATTTTGTAGGTCGGCTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGGGTAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGCCGGTTATGCCGCATCCGCCAT‑AACACCTGGGGCGCGCGTTTAACGCGTCTTTTCCAGCCTACCCTAGa                                                                                                                                                                                                                                                                                 >  1:160819/1‑301 (MQ=11)                     gATAAAGCGTTTATGCCGCATACGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAGAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCGTATCAGGCCTACGCCAAACCGCGCACTAGCCTCATCTAGCGTGCTTTTCTAGTTCGCATAACGCGTTTACGCCCCATCCGACATTACTGCCTGCGTCC‑AGGCTTGCCGCGTCGTATCAGACCTaacataa                                                                                                                                                                                                                                                                                  >  2:727330/1‑294 (MQ=11)                      aTAAGGCTTTTCTGCTGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGAc                                                                                                                                                                                                                                                                                <  1:460007/301‑1 (MQ=25)                            tgtttaggactgcgcagggatcgtggCCTGATGCGACGCTTAAGGCGTCTGATCAGGCCTACGCGTGACGTGGTACTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAGGGCTTTTACGCCGCATCCGGCATCAACGCCTGATGCGACGGTTGCCGCGTCTTATCAGGCCTAGGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAgcgc                                                                                                                                                                                                                                                                           <  1:210622/275‑1 (MQ=11)                            cGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCCTACCGCGCAATAGCCTGATTTAGCGCGATTTTGTAAGTCGGATAAGCCCTTTATGCCGCCTCCTGCATCCACTCCTGATGCG‑GCGCTTACCGCCACTTATCATCCCTACCCCAAACAgcac                                                                                                                                                                                                                                                                           >  1:481620/1‑298 (MQ=11)                                                          aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTACCGCGTCTTATCAGGCCTACGCCAGCCAGCGCCATAGCCTGATTTAGCGTGCTTTTGTAGGt                                                                                                                                                                                                                                             >  1:170305/1‑300 (MQ=25)                                                          aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCCGCCAGCGCAATAGCCTTCTTTAGCTTTGTTTTGTCGGt                                                                                                                                                                                                                                             >  1:93918/1‑300 (MQ=255)                                                            gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCCGCCAGACAGCGCAATAGCCTGAATTCGCGTGATTTTGTAGGTCGGATAATTCTTTTCTGCCGCATCCGACCTCAACGCCTCATTCG‑ACGCTTCCCGCCTCTTATCAGGCCTACGCCACACATCCCACTAGACTGATTTATAGTGACTTTGTAGGt                                                                                                                                                                                                                                             >  2:869688/1‑298 (MQ=11)                                                                         cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATATCCTGCTTTCGCGTGTTTTTTTAGTTCGGATAAGGCGTTTa                                                                                                                                                                                                                              >  1:847750/1‑300 (MQ=12)                                                                         cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTAGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCTGCCGCATGCGTCATCAACGCCTAATGCG‑AGGCTAAAGGCGTCTTATCAGGCCTACGCCAAATCGCGCAATAGCCGGATCACGCGTGATTTTGTAGGTCGGATCAGTCGTTTaa                                                                                                                                                                                                                             >  1:641517/1‑300 (MQ=11)                                                                            cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTACGCCAGCAAGCGCAAGAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCACCGCCTGCTGCT‑ACGCTTATCGCGTCTTTTCAGGCCTCCTCCACCCACCTCAATAGCCTGCCTTACCTTGGTTTGTTACGTCggtaacgccgttaccgc                                                                                                                                                                                                                           >  2:321046/1‑285 (MQ=11)                                                                              tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATGTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATAAGGCCTACGCACGACAGGGAAATATCACTCTTTAGAGTGATTTTGACATGCGGAGAAGGCGtttccgacg                                                                                                                                                                                                                         >  2:18994/1‑294 (MQ=11)                                                                                   ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATACGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACTCCAGACAGCGCAAGAGCCTGATTTAGCGTGACTTTGTAGGTCGGTTAAGGCGTTTACGCCGGATCCg                                                                                                                                                                                                                   >  1:257157/1‑301 (MQ=255)                                                                                        aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTGCTTTTGTAGGTCTGATAAGGCGTTTCTGCCGCATCCGACATCAATGCCTGATGCG‑ACTCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATACTGCTTTAGCGTGATCTTGTCTGTCCGACACGTCGTTTACTCATCATCCCACAtt                                                                                                                                                                                                              >  1:672944/1‑300 (MQ=11)                                                                                        aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCTGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACTCTTAACGCGTCTTATCAGGCCTCTTCCAGACACCGCACTAGCTTGATTTAGCTTGATTTTGTCGGTCGGATACGGCGTTTTCGACGCCTCCGACAt                                                                                                                                                                                                               >  1:645554/1‑300 (MQ=11)                                                                                             cgACAGCGCAATGGCCTGATTTAGCGTGTTTTTGTAGGGCGGATAAGGTGTTTACGCCGCATCCGACATCAACGCCTGCTGCGACGCTTGCCGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc                                                                                                                                                                                                         <  1:235175/300‑1 (MQ=255)                                                                                              aaCACCGCAAAAGGCTGAGTAAGCTGGAGTTTGGAGGGCGGAGAAGTCTGTTACGCCTCATCCACAATAAGCGCTGGATGCGAAGCTTGCCGCGTCTTATCAGGCCTATGCCAGACAGCGCAATAGCTTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc                                                                                                                                                                                                        <  1:937806/300‑1 (MQ=11)                                                                                                  gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGCTGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGCTTTCGCGTGATCTTGTCGGTCGGAGAAGACGTTTACGCCCCGACCGACCACAATGCCTGaa                                                                                                                                                                                                    >  2:230936/1‑300 (MQ=11)                                                                                                  gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGCGAGTTTGTATGCCGGATAAGCACTTTATGCCCCAGCCGCCATCAACACCCGaa                                                                                                                                                                                                    >  2:576798/1‑300 (MQ=11)                                                                                                  gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCCGAATAGCCTGATGTCGCGTGATTTTGTAGGTCGGCTAAGGCGTTTACGCCCACGCCGACATCAAAGCCTGAt                                                                                                                                                                                                    >  1:950838/1‑301 (MQ=11)                                                                                                     cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATCCCTGATGCg                                                                                                                                                                                                 >  1:976896/1‑301 (MQ=21)                                                                                                              gATTTAGCGTGATTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTCCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCCGCCGCATCCGACATCAATGCCTGCTGCGACGCttgc                                                                                                                                                                                         >  1:583035/1‑298 (MQ=255)                                                                                                                         aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACTTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGCCCTACGCCAGACAGCGCAATAGCATGTTTTTGCGTGCTTTTGTAGTTCGGATCAATAGTTATCGCCCCATCCCACATCAATGACTCATTCGACCCttgccggtccctataa                                                                                                                                                                             >  2:790336/1‑287 (MQ=11)                                                                                                                            ttGGAGGTCGGATTATGCGTTTACCCCGACTCCGCTAGCCACCCCGGAGGCTACGCTGTCCGCGTCTGATCAGGCCTAGGCCAGACAGCGCAATAGCCTGATTTAGCGGGATTTTGTAGGTCGGATAAGGCGTGTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                          <  2:170302/301‑1 (MQ=11)                                                                                                                             tgttgggcagaaacggCGTTGACGCCGTCTCCGACATCACAGCCGTATGAGGCGCTTACCAGGTCGTATCAGGCCTACGCCAGCAAGAGCAATAGCCTGATTTAGCGTGATTTTTTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGAG‑ACGCTTAAGGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                         <  2:301571/287‑1 (MQ=11)                                                                                                                                   tgggATAAGGTGTTTACGCCGCATCCGACAGCAACGCTAGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                                                                    <  1:283563/298‑4 (MQ=255)                                                                                                                                   gctgATGAGGCTTTGACTCCGCATCCGACATTAACGCCTGTTGCGACGCGGTCCTCGTCTTATCAGGCACACGCCACACAGCGCAATCGCCTGATTTAGAGTGAGTTTGTAGGTCGGATAAGTCTTTTATGCCGCATCCGACATCAACTCCTTATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGAGTGATTTCTTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATTCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                                                                    <  1:96049/297‑4 (MQ=11)                                                                                                                                   gcGGATAGGGTGTTTACTCCGCAGGCGAGATGAGCGCCGGAGGCGCCGCTGGCCGCGTCTTATCAGGGCTACGCCAGACAGCGCAATAGGCGGATTTAGCGTGATTTTGTTGGTCGGATAAGGCGTTGATGCCGCATCCGACATCAACGCGTGATGCG‑ACGCTGAACGCGTCGTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                                                                    <  1:986863/299‑4 (MQ=11)                                                                                                                                           ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGCTCGGATACGGCGTTTACGCCGCATCCGACATCCATCCCTGCTGCGTCGCTTGCCGCGTCTTTTCTGGCCTatcttcaccg                                                                                                                                                              >  2:10250/1‑289 (MQ=11)                                                                                                                                                                                                                                          aTTTTGTAGGTCTTATAAGTCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGC‑‑CGC‑ATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                          <  1:576807/300‑1 (MQ=2)                                                                                                                                                                                                                                                        acaaggcgtctcgcctgCACCCGGCATCACCGCCGATTGCG‑ACCCTTTCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGTATAAGGCGTTGATGCCGCATCCGACATCAACGCCGGATGCGACGCTTAACTCGTCTTATC‑GGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                           <  1:65807/284‑1 (MQ=11)                                                                                                                                                                                                                                                        aTAAGGCTTTTACTCCGCCTTCGCAGTCAACGCCTGATGCT‑ACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCTGATAAGGCTTTTATGCCGGATCCGACATCAACGTCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CGGCGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                           <  1:567458/301‑1 (MQ=11)                                                                                                                                                                                                                                                        aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                           <  1:702641/301‑1 (MQ=2)                                                                                                                                                                                                                                                         tacggcgttgccgCCGCATCCTCCACCACCGCCTGTTTCG‑CCCCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                          <  1:18996/289‑1 (MQ=11)                                                                                                                                                                                                                                                            ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCCGGCCTACGCCCGACCGCGCAATAGCCTGCTTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGC‑‑CCC‑CTCCGCCATCAATCCCTGATGCGACGCTTGCCGCCTCTTTTCAGTCCTATCTTAACCGTTg                                       >  2:325287/1‑301 (MQ=11)                                                                                                                                                                                                                                                                    tGCCGCACCCGACAACAACTCCCAGTGCG‑ATGCTTAACGGGTCTTCTTAGGCTGACGGCAGACAGCGCAAGAGCCTGATTGAGCGTGATTTGGGTGTTCGGATAAGGCGTTTAGTACGCTTCCGACACCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTCAAttt                               <  1:727353/301‑1 (MQ=11)                                                                                                                                                                                                                                                                      ccGCATCCGACATCAACGCCTGATGCG‑ACGCTTGCCGCGTCTTATCAGGCCTACGACAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGACGTTTATGCCGCAGCAGACATTAACGCCTGATGCGCCGATTAACGCGTCTTATCAGGCCTGCGCCAGACAGCGCAATCGCCTTCCTGATCGTCACTTTGTGGTTCGGATAAGGCGTTTA‑‑CG‑AACAATCCAACATCCATGCTTGATGCACAGCTTGCCCCGTCTTATAACCCGTCTCTTACCCGTTGCTTACttctt                             >  2:686958/1‑301 (MQ=2)                                                                                                                                                                                                                                                                                cATCAACGCCTGATGCG‑ACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCTGATCAGGCGTTTACGC‑‑CGC‑ATCAGACATCAATGCCTGATGCGCCGCTTTCCGCGCCTTATCCGGCCTCTCTTAACCGTTGGTTAATTTTTCAAACTCtt                    >  2:35968/1‑300 (MQ=11)                                                                                                                                                                                                                                                                                 aTCAACGCCTGATGCG‑ACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTGCTTTTGTAGGTCGGCTAATGCGTTTACGC‑‑CGC‑ATCCGACATCAATTCCTGCTGCGACGCTTGCCGCGTCTTCTCGTGCCTCTGTTACCCGTTGATTCATTTTTCACACTCTTc                   >  1:803739/1‑300 (MQ=11)                                                                                                                                                                                                                                                                                            aggCG‑AAGCGTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGTGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                          <  1:870390/264‑1 (MQ=11)                                                                                                                                                                                                                                                                                            aTGCG‑ACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGTTAt        >  1:184299/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            aTGCG‑ACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCATGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTCACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTAt        >  1:532607/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  aCGCTTGCCGCGTCTGATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc  <  1:211845/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         TAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCG‑ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACC  >  NC_000913/4295896‑4296482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.