Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,907,562	IS1 (+) +9 bp	100%	coding (22‑30/144 nt)	yobF ←	DUF2527 family heat‑induced protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	153 (1.150)	88/550	NT	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	1907562 =	0 (0.000)					coding (30/144 nt)	yobF	DUF2527 family heat‑induced protein
*	?	NC_000913	= 1907570	0 (0.000)	121 (0.910)	84/550	NT	100%	coding (22/144 nt)	yobF	DUF2527 family heat‑induced protein
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

AACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTC  >  NC_000913/257619‑257918                                                                                                                                                                                                                                                                                                  |           aaagATCCTTGCAGCCCGGAAGAGGACTCTCGCTTACTCACCTCATAATCTGCGGACGGTAAATTATTAGAGACCCCATTTAACCCTGTGTATCGGAAGTGTGACAAAGGTCTTAAGGAAATCACCGACTGGATCTAAAAACGCTTTTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAaggtaagtgcaaagat  <  1:602425/297‑9 (MQ=255)                                                                                                                                                                                                                                                                                                  |           AACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTC  >  NC_000913/257619‑257918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.