Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,815,808 (A)8→7 intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8010A.97.4% 159.8 / ‑2.9 40intergenic (‑33/+33)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (19/19);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

CCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTT  >  NC_000913/3815519‑3816097
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        
ccACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACtcatc                                                                                                                                                                                                                                                                                        >  2:310889/1‑300 (MQ=255)
        gTTGTGGTGGCAATCGGGATCCCTTTTTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTGATGAGTCGCCtt                                                                                                                                                                                                                                                                               <  2:396442/301‑1 (MQ=255)
            gggtggCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGAGCGAACTCAATGCAGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTGATGAGTCGCCtttttt                                                                                                                                                                                                                                                                           <  2:236492/300‑5 (MQ=255)
               gggCAATCGGGATCCCTTTGTCAGCAGGGCCAAACAGCAGATCGAACTCCATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATc                                                                                                                                                                                                                                                                        <  1:310800/300‑1 (MQ=255)
               gggCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATc                                                                                                                                                                                                                                                                        <  2:47571/300‑1 (MQ=255)
                  cAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGt                                                                                                                                                                                                                                                                     >  2:35706/1‑301 (MQ=255)
                                                acgtgggctctcccgatctGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACaa                                                                                                                                                                                                                                       <  2:265252/283‑1 (MQ=255)
                                                 ccacccaccaaaccctctGCCCGCATCCACACAGGCTTCAGCGTAAACACGCCCTTACAGTCCCAGATGGCATCCGGTATTAGACAACCCGGAGTGGAAAAAATAGGGGCGTTTGCTCCCTGTCTGCACCGTAATCGCGCCAAACTTTAACTCCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGTTGAAATGGTTTCATGCCTTCGCTCCTCATCTTACTTGTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTGGCCAGCGCCGCCTTCTGCGTCGCTACAAt                                                                                                                                                                                                                                      <  2:111834/284‑1 (MQ=255)
                                                          gAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCCCCGCCTTCTGCGTCGCTCCAATGgattcgct                                                                                                                                                                                                                             >  1:44547/1‑299 (MQ=255)
                                                          aaaCTCAATTCCTGAAACCACCCACGCTTAAACGTAAAAACGGCATAACAGTGCCCGATCGAGCCCGGTATTAAACAGCCCGGAGTTGAAGAAATAGGGGCTTTTGCGCACGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCGAAGCGCAGATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgat                                                                                                                                                                                                                             <  1:312540/300‑1 (MQ=255)
                                                                   gCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTCCTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTCTTCCCGCGCCGCCTTCTGCGTCGCTACaaagatcgtaagagcgacc                                                                                                                                                                                                                      >  1:265175/1‑282 (MQ=255)
                                                                                   gCTTCAGCGTAAAACCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTGATGAGTCGCCtttttt                                                                                                                                                                                                                                                                           <  1:353173/230‑5 (MQ=255)
                                                                                   gCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTGATGAGTCGCCtttttt                                                                                                                                                                                                                                                                           >  2:353268/1‑226 (MQ=255)
                                                                                      tCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAACCTTGACAAACTTTAACACCTGCTTGCTAATCGCAACTTTAATAAACTAGCGCTGATATGGTTTCATTCCTTCGCTCCGCATTTTACTTTTCTTCATAAAAAATAATGCGATTACTCAAACGCCTTAAAAATCAGTTTGCCCGCGCCGTTATCTGCTTCTCTCAAATTGATTCGATTCCCTCCGGGCCTGGTCcaacaaagg                                                                                                                                                                                                   >  1:169811/1‑296 (MQ=255)
                                                                                                   ggCCTAAAAGTGCCAGATCGCGCCCTGTAATAAACAGCCCTGCGTTGAAGAAATAGTGTCATTTGCACCCGGATATCAGAGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                                                                                                    <  2:169861/301‑1 (MQ=255)
                                                                                                              gCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCCACAATGGTATCGATTCCCCTCGGGCCAGAGCCAACAACATGAGTAGCTCTCCCTGGGTGCACGGCt                                                                                                                                                                         >  1:331123/1‑301 (MQ=255)
                                                                                                                                  aaaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTAGCCTTAAAAATCAGTTTGCCAGCCACGCCTTCTGCGTCGCTACAATTGATTCGATTCCCCTCGGGCCAGAACCCACAAGATGAGGAGCTCTTCATGGGTGAACGCCTCGCCTTCTGCCGTccccctc                                                                                                                                                     >  2:96651/1‑298 (MQ=255)
                                                                                                                                       gCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATggg                                                                                                                                                                                  <  2:218186/267‑1 (MQ=255)
                                                                                                                                       gCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGAGAGATCggaagagggtcgtgtggggaaagggggtg                                                                                                                                                >  1:218122/1‑274 (MQ=255)
                                                                                                                                            gcgttgcattaatcggctcttctcgccccGGAGTTCAGCGTCAAATCGCCCAACTTTAACTCCTTCTTGGTATGCTCAAATTCTCTAACCTTCCGCTGCTTTGTTTTCATTCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAACAAGCCGACTCATCTGTCGCCTTAAAAATCATTTTGCCAGCTCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCatt                                                                                                                                             <  2:100970/273‑3 (MQ=255)
                                                                                                                                               ttGAAGAAATATGGGCGTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                                                                                                         <  2:44559/300‑1 (MQ=255)
                                                                                                                                                     aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATTCGcccct                                                                                                                                  >  2:36285/1‑299 (MQ=255)
                                                                                                                                                         agaggCTTTTGCGCCCGGATTTCAGCGTAACCTCGCCAAACTTTAGCACCGTCTTGCTAAGCGACAATTTCATAAACGTGAGCTGATATGGTTTCATGCATTCGCTCCTCATCTTATTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTc                                                                                                                              <  1:281104/298‑1 (MQ=255)
                                                                                                                                                                 ttGCGCCCTGATTTCAGCGTACACTCGCCAACCTTTAACACCTGCTTGCTAAACTCAAATTCAACAAACTGGCGCTGATATGGTTTCATCCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatc                                                                                                                       <  1:35696/300‑1 (MQ=255)
                                                                                                                                                                         ggATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTTCCGTCCCCGGCACTTCAATTATGCGCCCCGCTTCGGTCCTCCCTAccgtc                                                                                                              >  1:327493/1‑297 (MQ=255)
                                                                                                                                                                              tCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTTGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                                                                                                                                                                      <  2:220197/240‑1 (MQ=255)
                                                                                                                                                                                     aaaCTCGCCAACCTTTAACACCTGCTTGTTAAGCGCAAATGCCATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCATCATCGTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTCAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGCCTTCGCTTCCCCTCGGCCCAGGCCCACCAAGATGAGTCGCTCTCCGTGGGTTAACGGCTCGCCCTCTACCGCCCCCTGCACTACCATGTTTCGCCCGCCTTcccgcctctccaccttcttgccgctctcc                                                                                                  >  1:269137/1‑273 (MQ=255)
                                                                                                                                                                                              aacatatAACACCTGCTTCCTCAGGCCAAATTCAATCAACTGGCTCAGATATGCTTTCATGCCTTCGCTCCTCATCTTACTTTTCTCCAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCagag                                                                                         <  1:36275/295‑1 (MQ=255)
                                                                                                                                                                                                   ttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGAGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGCGCCAACAAGATGAGAAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGAGGCGCACGACTTCGGTACGCACGACGTTCATGTCGGTCTCGGCGGCAGAGGCTTc                                                                                    >  2:148607/1‑301 (MQ=255)
                                                                                                                                                                                                    tAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCCCTACGTTCCTGTCGGTCTCTGCGGCCGAGTCTTc                                                                                    >  2:92948/1‑300 (MQ=255)
                                                                                                                                                                                                       cacCTGGTTTCTAAGCACAAATTCATACATATGGATCTGCTGTTGTTTAAGTCATTCTCTCCTCATCTTACTTGTCCAACGACAAAAAAATGCTACTCATCAGTCGCTTTATAAATCAGTTTGCCAGCGCCTCTTTCTTCGTCTCTACAATGGATTCGATCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAGGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACg                                                                                <  2:285747/301‑1 (MQ=255)
                                                                                                                                                                                                          cTGCTTGCTAAGCGCAAATTCAATCAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCAt                                                                                                            <  1:55936/270‑1 (MQ=255)
                                                                                                                                                                                                          cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCCCTACGTTCAt                                                                                                            >  2:55952/1‑270 (MQ=255)
                                                                                                                                                                                                                   atgaccAAATTCAAAAAACTGCCTCTGATATGGTTTCATGCCTTCGCTCCTCAGCTTACTTTTCTACAGCCAAAAAAAGGCGACTCATCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACg                                                                                <  1:91806/284‑1 (MQ=255)
                                                                                                                                                                                                                   aaGCGCAAATTCAATAAACTGGCTCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACg                                                                                >  2:91830/1‑289 (MQ=255)
                                                                                                                                                                                                                                   accgcgtccTGATATTGTTTCATTCCTTCCCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCCGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGt                                                    <  1:92924/293‑1 (MQ=255)
                                                                                                                                                                                                                                                            cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCCCAATTCCGCACGAAACTGCGGc                           >  1:67637/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                gCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGATTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCCACAGAACCTGCGGCTAcc                       >  1:194475/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                           tACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCAt            >  2:317581/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                     cAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCCCTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGCGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGCTTGGtt  >  1:196340/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        
CCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTT  >  NC_000913/3815519‑3816097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: