Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 58 [0] [0] 58 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)58 (1.170) 48/556 0.1 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

TGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGACAGCAGGCTCATAAGACGCCCCAGCGTCGCCATAGTGCG  >  NC_000913/257613‑258195
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                
gggctgaacggatcgttcagcccggaaGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGTTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACAACCGAAGAGCACGTTGATCCAAAAAATTACTGCACCAGGCCCCTCTATTCGAGAAGGTAagtgcg                                                                                                                                                                                                                                                                                            >  2:285199‑M1/14‑295 (MQ=255)
 ggAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgca                                                                                                                                                                                                                                                                                            >  2:122064‑M1/1‑294 (MQ=255)
                 cAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACACGAAGAGCAGATTGATCAAAAAATTTACCGCACAAGGCTCGTATATTTGCGAAGGTAagtcaaaagataatcactacttt                                                                                                                                                                                                                                                                           >  1:253121‑M1/1‑278 (MQ=255)
                               aCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGACATAGCAATGAAGTTACCGAGTGGACACACGACGAGCAGATTGATCAAGAAATTTACCGCACAAGGCCCGTATATTCGTGAAGTTAagtgaaaagataaacgattctttttcgattgtctgg                                                                                                                                                                                                                                                              >  1:101726‑M1/1‑264 (MQ=255)
                                             aCCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgagatggg                                                                                                                                                                                                                                                >  2:290451‑M1/1‑250 (MQ=255)
                                             aCCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatg                                                                                                                                                                                                                                                       <  1:290365‑M1/293‑44 (MQ=255)
                                             aCCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtatggctgtatgcgtcaacg                                                                                                                                                                                                                                               >  2:241017‑M1/1‑250 (MQ=255)
                                              ccTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagatactcgatgctttttcgattgtctggctgtatgcgttaacgt                                                                                                                                                                                                                                              >  2:140534‑M1/1‑249 (MQ=255)
                                              ccTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATGGATCAAAAAATTTACCGCACTAGGCCCGTATATTAGTGAAGGTAatggcaaagataatcgaatcttttcagattgtctggctgtagccggcaccgt                                                                                                                                                                                                                                              >  1:426493‑M1/1‑249 (MQ=255)
                                                     atcTGCTGACTGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGAATGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                                                                                                                                                                        <  1:425437‑M1/300‑59 (MQ=255)
                                                        tgcggtaggtaaattaTTAGAGGTCCAATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTAAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggc                                                                                                                                                                                                                                     <  1:66391‑M1/293‑62 (MQ=255)
                                                           ggaCGTTAAATTATTAGAAATCCCATTTAACCCGGTGTATCGGAAATTTAAAAAAGGTCTTAAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcacc                                                                                                                                                                                                                                  <  1:240944‑M1/299‑65 (MQ=255)
                                                                              aTCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGACGGTAagtgcaaagataatcgattctttttcgattgtatggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggg                                                                                                                                                                                                              >  2:10837‑M1/1‑217 (MQ=255)
                                                                              aTCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgtatgtatgcgtcaacgggaaaacggcacgggaagtgagtgaattcgggg                                                                                                                                                                                                              >  1:16309‑M1/1‑217 (MQ=255)
                                                                                   aTTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtg                                                                                                                                                                                                                       >  2:91011‑M1/1‑212 (MQ=255)
                                                                                   aTTTAACCCGGTGTATAGGAATTTTGACAAAGGTATTCAGGAAATAACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtg                                                                                                                                                                                                                       <  1:90987‑M1/287‑76 (MQ=255)
                                                                                    tttAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacg                                                                                                                                                                                                                                               >  2:134577‑M1/1‑211 (MQ=255)
                                                                                    tttAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacg                                                                                                                                                                                                                                               <  1:134544‑M1/262‑52 (MQ=255)
                                                                                             gtgtATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgcgaattctggggctggtggctggggg                                                                                                                                                                                               >  2:54434‑M1/1‑202 (MQ=255)
                                                                                              ggtaTCGGAATTATGACAAAGGTATTCAGGAAATCACCGGCTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACAACCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagct                                                                                                                                                                                              <  2:246806‑M1/300‑101 (MQ=255)
                                                                                                    gtaattTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagc                                                                                                                                                                                        <  2:391621‑M1/299‑107 (MQ=255)
                                                                                                    ggAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCTCACTAGTCCCGTATATTCGTGAGGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcacctgatttgcgtgtattctgaggcgtgtgtgggtagctggacgg                                                                                                                                                                                        >  2:189708‑M1/1‑195 (MQ=255)
                                                                                                     gAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATAGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                                                                                                                                                                       <  2:67497‑M1/301‑108 (MQ=255)
                                                                                                       aTTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggatgtatgcgtcaacgtgaaaccggcaccggaagtgcgttaattctggtgctggtggatggaggtggaagcgcg                                                                                                                                                                                     >  2:143658‑M1/1‑192 (MQ=255)
                                                                                                              cAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggaaccggaagttcgtgactgctggggctggtgggtggggctggaagcgcagggatcc                                                                                                                                                                              >  1:264667‑M1/1‑185 (MQ=255)
                                                                                                                  ggttgttaagaaatcaccgACTGGATCGAAAAACGCTTGTTGTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGTATCACAACAGGGGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAAATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtt                                                                                                                                                                          <  2:253197‑M1/292‑121 (MQ=255)
                                                                                                                   ggtttcagGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttt                                                                                                                                                                         <  2:264744‑M1/298‑122 (MQ=255)
                                                                                                                     cTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTAGTGAAGGTAagtgcaaagataatcgattctttttcgatagtctggctgtatgtgttaaggtgaaaccggcaccggaagtgcgtgaactctggggatggtggagggagagggaggggaaggaactgagtgttg                                                                                                                                                                       >  1:174259‑M1/1‑178 (MQ=255)
                                                                                                                                   cggctggTTCGAAAAACGCTTGTTTTAAAAATTTGCCAAATTTTGCCAATTTGGTAAAACAGTTGCAGCACAACAGGGGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccag                                                                                                                                                         <  1:54418‑M1/298‑138 (MQ=255)
                                                                                                                                    gACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtttggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggatgtggaagcgcaggaatcccgttgtagctacagttacc                                                                                                                                                           >  1:209111‑M1/1‑163 (MQ=255)
                                                                                                                                    gACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGAAAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccag                                                                                                                                                         <  2:209172‑M1/300‑138 (MQ=255)
                                                                                                                                        ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttgg                                                                                                                                                    <  1:122036‑M1/301‑143 (MQ=255)
                                                                                                                                                       tgggttaaAAATTTGCGAAACTTTGCCAAATTGGTAAAACAGTTACATAACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaag                                                                                                                                      <  2:224217‑M1/297‑157 (MQ=255)
                                                                                                                                                         tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgggggatggagctggaagcgcaggaatcccgttttacttacagttaccggtttggtctgttcgataaagc                                                                                                                                     >  2:85958‑M1/1‑142 (MQ=255)
                                                                                                                                                                      cTAAATTTTGCCAATTTGGTAAAACAGTTGCAGCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGACGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATCTTCGTGAAGGTAagtgcacagataatcgattctgtttcgattgtctgactttatgcgccaacgtgaaagcggcaacggaagtgagtggattctggggctggaggatggagctgcgagcgcgggaatgccggtgtagctaacggtgagagtttggtcggttcgataaagaaggctactagaaga                                                                                                                       >  1:352929‑M1/1‑129 (MQ=255)
                                                                                                                                                                       tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggtgctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgttaatgcaggagactggaagagg                                                                                                                     >  2:418484‑M1/1‑128 (MQ=255)
                                                                                                                                                                        acattGTGCCAATTATGTAAAACAGTGGCATGACAACAAGAGATGGCAATGACGTTACCGAGTGGACACCCGAAGGGCAGATTTATCAAAAAATTTACCGCACTAGTCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagag                                                                                                                      <  1:10836‑M1/297‑173 (MQ=255)
                                                                                                                                                                                ccAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                                                                                                                                                               >  2:197194‑M1/1‑119 (MQ=255)
                                                                                                                                                                                ccAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                                                                                                                                                               <  1:197137‑M1/202‑84 (MQ=255)
                                                                                                                                                                                   attaggacaaacaattgctcactcaaagtagaaagcaatgaagttacagaaaggacacccgAAGACAAACTTGATCAAAAAATTTACCGCACCAGGCCCGTATATTCGTGAAGGTAaggtcgaagataatcaattctttttcgtgtgtctggctgtatgcgtcaacatgaaaccggcaccggaagtgcgtgaattctggtgcttgtggatgtagctggaagcgcatgaatcccgttttacctacagttaccagtttggtctggtcgataaagcaggcgactggaagagtgttccggtaaaa                                                                                                         <  1:285112‑M1/272‑186 (MQ=255)
                                                                                                                                                                                              cAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtc                                                                                                                                                                                                                                                   <  1:313879‑M1/152‑48 (MQ=255)
                                                                                                                                                                                              cAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtc                                                                                                                                                                                                                                                   >  2:313968‑M1/1‑105 (MQ=255)
                                                                                                                                                                                                          cacacgAGATAGCAATTACATTACCGAGGGGACACCCGAAGAGCGGAATGATCAAAAAATTTACCGAACTAGTCCCGTATATTCGTGAAAGTAagtgccaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgact                                                                                  <  1:85935‑M1/300‑209 (MQ=255)
                                                                                                                                                                                                            caGGAGATAGCAATGACGTTACCGAGTGGACACCAGAAGAGCAGATTGATCAATAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagctaatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactgg                                                                                <  2:353024‑M1/301‑211 (MQ=255)
                                                                                                                                                                                                               cctttccctacacgccgcctttccgatctcccgaagagcagattggtaaaaaaatttaccgCACTAGGCCCGTATATTCGTGAAGGGAggtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagc                                                                             <  1:99407‑M1/272‑214 (MQ=255)
                                                                                                                                                                                                                agaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaatgaaacggaaggggttgaacgaagggagc                                                                             >  2:11882‑M1/1‑87 (MQ=255)
                                                                                                                                                                                                                   tAGCAAGGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagag                                                                                                                      <  1:128796‑M1/256‑173 (MQ=255)
                                                                                                                                                                                                                     gCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttcaggtaaaagaaactgaagtggttgaacgactggagcacaccc                                                                       >  1:265084‑M1/1‑82 (MQ=255)
                                                                                                                                                                                                                         cgccggtaccgagTCGACAACATGAGATCATGTTGAACAAAAAATTATCCGCACTAGGCCCGTATTTGCGTCAAAGTAagtgcaaatgtaatcgattcgttttcgatggtcttgctgtatgcggtaacgtgaaaccggcaccggaagcgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcg                                                                   <  2:174311‑M1/295‑224 (MQ=255)
                                                                                                                                                                                                                                        gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagca                                                                            <  2:190650‑M1/277‑215 (MQ=255)
                                                                                                                                                                                                                                        gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagaaaatgaagtggttgaacgactggagca                                                                            >  1:190593‑M1/1‑63 (MQ=255)
                                                                                                                                                                                                                                            cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgttaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacatacagttaccagattggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgagtggagcacaccgtgcgtgagtttcacgataagatg                                                >  2:31863‑M1/1‑59 (MQ=255)
                                                                                                                                                                                                                                            cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcagataggaagaacacacgtctgaagtcc                                                 >  2:99432‑M1/1‑59 (MQ=255)
                                                                                                                                                                                                                                              caaagaGCAGATTGATCAAAAAAGTTACCTCACTAGGCCCTTATATTCGTGAAGGTAagtgcaaagataatcgatgctgtttcgattgtctggatgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtttcacgagaagctgcg                                              <  2:16311‑M1/299‑245 (MQ=255)
                                                                                                                                                                                                                                              cGAAGAGCAGATTGAGCAAAAAATTTTCCTCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtttcacgagaagctgcg                                              <  2:183418‑M1/301‑245 (MQ=255)
                                                                                                                                                                                                                                                 agagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATGCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtttcacgagaagctgcgtga                                           <  2:265161‑M1/301‑248 (MQ=255)
                                                                                                                                                                                                                                                                           gCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgatcaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtttcacgataagctgcgtgaatgtctgacgacgcttaatctgaatc                 >  2:396949‑M1/1‑28 (MQ=255)
                                                                                                                                                                                                                                                                                           ttCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtttcacgagaagctgcgtgaactgctgacgacgctgaagccgaagatggaaccggcggatg  >  2:128290‑M1/1‑12 (MQ=255)
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                
TGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGACAGCAGGCTCATAAGACGCCCCAGCGTCGCCATAGTGCG  >  NC_000913/257613‑258195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: