Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,529,218	C→T	pseudogene (1317/2037 nt)	rhsE →	pseudogene, Rhs family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,529,218	0	C	T	100.0%	113.8 / NA	41	pseudogene (1317/2037 nt)	rhsE	pseudogene, Rhs family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/18);  total (23/18)

GGTCGAGACAGAGAACGGCGAGCGGGAAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGAC  >  NC_000913/1528921‑1529508                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                   ggTCGGGACGGGGAACGGCGGGCGGGAAAAAGCGCGGGGGCGCGGGCTGGCAGAGAGGCTCCAGACGGAAGGGAGTGAGAACGGCCACGGCGGGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCTGGCGTGGCTGGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCttatc                                                                                                                                                                                                                                                                                                 <  1:375059/301‑1 (MQ=11)           gagaACGGCGAGCGGGAAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCC‑TTTTTACCACTTCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGCCg                                                                                                                                                                                                                                                                                       >  2:421920/1‑301 (MQ=17)             gaAGGGCGGGGGGGAAAAAGGGCAGCGGCGGAGCCGGGGAGAGCCGCTCCAGCGGGAAGGGGGTGGGAACGGCCACGGCGGGGTGTTCCCGGCTGAACTGGGGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGc                                                                                                                                                                                                                                                                                     <  1:166663/301‑1 (MQ=11)                        gggAAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACCGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGTCTTCTGGACTGGATGGAGGACGCAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCGTACGTAATGCGGGCTGCAGCTCGGCCAAATGGCCATTCAGGCCGGTTCGGCCATCACACCTACGCTAAAAGGCCAGTTTTCACCTC‑GCACCCCCCGGGGCCGGCCGCTGGCGCTTGTCACTGAAGACGGcgactgggcatt                                                                                                                                                                                                                                                                          >  1:177404/1‑290 (MQ=2)                                               cTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCCGCGAAGACGGCAATACGGCGTGGCGGGGGGGGGATGATGAATggg                                                                                                                                                                                                                                                   >  2:438083/1‑301 (MQ=255)                                                          ccaccAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCtt                                                                                                                                                                                                                                        <  2:394670/298‑1 (MQ=25)                                                                           gggaagaCGGCCACGGCGTGGGGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCa                                                                                                                                                                                                                       <  2:218284/295‑1 (MQ=25)                                                                             gagaACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCATGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATAGATGGGGCAACCAGCGTAATGGGGAGAACCCGCATc                                                                                                                                                                                                                     >  2:22619/1‑301 (MQ=255)                                                                               gaACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAc                                                                                                                                                                                                                   >  2:317531/1‑301 (MQ=37)                                                                                                   cccggggtgactgggTGGGGGTGCTGGACAGGCGGGGGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCt                                                                                                                                                                                               <  1:109740/288‑1 (MQ=255)                                                                                                          ggAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAggg                                                                                                                                                                                        <  2:366627/300‑1 (MQ=255)                                                                                                                                 ggCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGAGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAACAGCGTAATGAGGAGAACCCGCATCACCCGCACCATCGGTTACGTCTGCCAGGGTAGCAGCATGATGAGGAGTCggg                                                                                                                                                                 >  2:304290/1‑301 (MQ=255)                                                                                                                                           agaaATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGCGTCGGGGCTGTACtat                                                                                                                                                       >  2:360146/1‑301 (MQ=255)                                                                                                                                                gccGGGCAGACCGGGTGAGCAGGGAAAGCCGGGCGTGGCTTGCGCAGGAGGGGCTGACGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCg                                                                                                                                                  <  1:360049/300‑1 (MQ=255)                                                                                                                                                                       gAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACCGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGTGTATGCTGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCCGCACCAGCCGTACCGTCTGCCAGCGCAGCTGCATGCTGAGGAGTCGGGGCTGGCCTCTAACCGTCCTCGGAACTACGATCCGTTGc                                                                                                                           >  2:261084/1‑301 (MQ=14)                                                                                                                                                                       gAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGcc                                                                                                                                                                                                        >  1:282532/1‑224 (MQ=255)                                                                                                                                                                       gAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGcc                                                                                                                                                                                                        <  2:282614/224‑1 (MQ=255)                                                                                                                                                                          aGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACGAGCTTAATGAGGAGAACGCGCATCACCTGCACCCGGCGTACCGTCTGCCAGGGCAGCCGCAAGACGAGGAGTCGGGGCTGTACTATAAGCGTCCCAGGCCCCACGATCACTTGCAgg                                                                                                                        >  2:36685/1‑301 (MQ=14)                                                                                                                                                                              gggCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGGGGAGTATGATGAATGGGGCAACTAGCTTAATGAGGAGAACCCGCAACACCTTCACCAGCCGTGCCGCCAGTCAGGGCAGTGGCTCGAGGCGGAGTCGGGGATGTAGTATTAGCGtacacggacctgggctcacttatggtggtg                                                                                                                    >  1:174810/1‑272 (MQ=14)                                                                                                                                                                                 cGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCCGGATGAGGAGGCGGGGCTGTACTATAACCGTCAACGGCCCTCCGAACCGTTggcggggcgggg                                                                                                                 >  1:317457/1‑299 (MQ=14)                                                                                                                                                                                    ggcgtgggaaGTGCGGGTTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTatat                                                                                                              <  2:1242/292‑1 (MQ=255)                                                                                                                                                                                                      ccccccacgctctcccgttctgACCGGTGGAGCCGGAACACCCACCGGGGCGAAAAGTTCA‑TTTTTCCCACTGCGACCACCGGGGCCTGCGGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTgg                                                                                            <  1:81758/280‑1 (MQ=17)                                                                                                                                                                                                       gACGGTGGAGCAACTGGCCAGACAGGTGGATCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGTGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCgg                                                                                                                                           <  2:135115/253‑1 (MQ=255)                                                                                                                                                                                                         cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGCGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTCCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTAATATAACCGTCCACGGCACTACGATCCGTTGGAAGGGAGGGATATCACCCCGGACCCGCTgggggg                                                                                         >  1:455311/1‑299 (MQ=14)                                                                                                                                                                                                                  aaCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCAc                                                                                                                                              >  2:377030/1‑239 (MQ=255)                                                                                                                                                                                                                     gggCCAGACGGGTGGAGCCGGAATACACACCGGGGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCCGCCGCTGGGGCTTATCAGGGAAGACGGCAATACGGCGTGGCGGGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGtggatg                                                                             <  1:264708/300‑1 (MQ=255)                                                                                                                                                                                                                        ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTAATATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGAGTGGGTTGAGGGGtgggtgga                                                                           >  2:401612/1‑300 (MQ=255)                                                                                                                                                                                                                           gACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTggagatcggaagagcaccgtctg                                                                       >  2:81781/1‑280 (MQ=255)                                                                                                                                                                                                                                                   cGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCAt                                               <  2:190155/301‑1 (MQ=255)                                                                                                                                                                                                                                                    ggCGCGAAAAGTTCA‑TTTTTACCCCTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCAc                                                                                                                                              <  1:265787/205‑1 (MQ=255)                                                                                                                                                                                                                                                    ggCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCAc                                                                                                                                              >  2:265864/1‑205 (MQ=255)                                                                                                                                                                                                                                                         gAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATCTCACCCCGGACCCGATTGGGTTGCGAGGGGGATGGAATATGTTTCAGTATCCGTTGAATCCCATACAAGt                                         >  2:343369/1‑301 (MQ=255)                                                                                                                                                                                                                                                            aggTTCA‑TTTTTACCACTGCGCCCCACGGGGCCTGCCGCTGGGGCTTATCAGCGAAGCCGGCAATACGGGGTGGCGCGGGGAGTATGGTGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGAt                                      <  1:317442/299‑1 (MQ=255)                                                                                                                                                                                                                                                             aGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCatgatg                                                                                                                                                                           <  1:348016/167‑1 (MQ=255)                                                                                                                                                                                                                                                             aGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCatgatg                                                                                                                                                                           >  2:348111/1‑167 (MQ=255)                                                                                                                                                                                                                                                                         ccacccgCGACCACCGGAGCCTGCCGCGGGCGCTTATCAGCGAAAACGGCAATACGGAGTGGCGCGGGTAGTATGATGAATGGGGCAACCAGCTGAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGGCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAAAATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATgg                           <  2:321108/294‑1 (MQ=18)                                                                                                                                                                                                                                                                          ccACTGCGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGCTCCGTTGCAGGGTCGGTATCTCACCCCGGACCCGAATGGTTTGAGAGGTGGATGGCATATGTATCAGTATCCGTTGAGTCCCATCCAAGTGCACAACCCAATgggg                         >  2:26123/1‑301 (MQ=18)                                                                                                                                                                                                                                                                                cGACCACCGGGGCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGtt                       >  2:284102/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                          ggCCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTgaga         <  2:317546/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ccTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGCGGAGAACCCGCATCACCTGCACCAGCCGTAACGTCTGCCAGGGCAGCAGCATGTTGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCTGTATATCACCCGTGACACGATTGGGTTGAGAGGTGGATGGAATATTTATCAGTATCCGTTGAAGCCCATACATGGGATAGACGAAACGGGGtaaaagccgttggagaat       >  2:429065/1‑284 (MQ=17)                                                                                                                                                                                                                                                                                            ccTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGGGGTGGATGGAATATGTATCAGTTGCCGTTGAATCACATACAAGGGGTGGACCCAGGGGGTATAGATGCGGTTGGGaa        >  1:88249/1‑300 (MQ=18)                                                                                                                                                                                                                                                                                                 cGCTGGCGGTTATCAGAGAAGACGGCAAGCGGGCGGGGGGCGGGGAGTATGGTGAATGGGGCAACCAGCGTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGGCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGAc  <  1:421809/301‑1 (MQ=25)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                   GGTCGAGACAGAGAACGGCGAGCGGGAAAAAGCGCAGCGGCGCAGCCTGGCAGAGACGCTCCAGCAGGAAGGGAGTGAGAACGGCCACGGCGTGGTGTTCCCGGCTGAACTGGTGCGGCTGCTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCA‑TTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAGTGATAGACCCAATGGGGTTAGATGCGATTGAGAATATGAC  >  NC_000913/1528921‑1529508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑