Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,967,417 G→A Q340* (CAG→TAG)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,967,4170GA97.4% 124.3 / NA 38Q340* (CAG→TAG) ptsPPEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/16);  minor base C (0/1);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGATC  >  NC_000913/2967126‑2967708
                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                    
cTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCCGCt                                                                                                                                                                                                                                                                                              >  2:58261/1‑300 (MQ=255)
  tttATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTc                                                                                                                                                                                                                                                                                             <  2:142490/299‑1 (MQ=255)
  tttACACGCCCCCCGCGTTTTAACTCATGGGGTAAATATACCACATCTTCCGCCAGACGGCTAAGCTCAATCTCCTCACAAATTAGCCGCTGATATTCTTGCAGCAGGACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCt                                                                                                                                                                                                                                                                                              <  1:58245/298‑1 (MQ=255)
                  tttttAACCGCGCGTGTAAAATGCCGGCCGTTCTCGCCAGACGGCTAAGCTCAAGCTCTTCACTACTTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCGACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTg                                                                                                                                                                                                                                                                            <  1:149314/300‑1 (MQ=255)
                      tacatGCGCGGGAAAATTACCGCCACCTTACGCCAGACGGCTAACCTCAACCTCTTCACCAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAg                                                                                                                                                                                                                                                                        <  2:357126/296‑1 (MQ=255)
                                                                 aGCTCAATCTCTTCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCCCAGCAAGCGTCGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGGGTTAGGcc                                                                                                                                                                                                                            >  1:165671/1‑301 (MQ=255)
                                                                         ccctgcACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGGCAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTa                                                                                                                                                                                                                    <  2:41816/296‑1 (MQ=255)
                                                                             tCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATGAGCgc                                                                                                                                                                                                                >  2:255006/1‑300 (MQ=255)
                                                                                     tAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTTGCGtcatca                                                                                                                                                                                                           >  2:374228/1‑298 (MQ=255)
                                                                                                atTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACa                                                                                                                                                                                                                                                                         <  2:5684/225‑1 (MQ=255)
                                                                                                     tGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTCGCGTCATCAAGAGGAAACAGCAATCgac                                                                                                                                                                                        >  2:364296/1‑299 (MQ=255)
                                                                                                                  gCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACCGCAACCGCTGACCCAGCGCACg                                                                                                                                                                           >  2:8204/1‑301 (MQ=255)
                                                                                                                           cGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGACAAAGCAATCGCTGACCCCGCGCCCGTaacacccc                                                                                                                                                                  >  1:371413/1‑295 (MQ=255)
                                                                                                                              ccAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGACGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCCACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAACTCATCTGCCACCAGAATGAAACTTTCCGGCCAAGCCTTCGGCCCTTCATTAGCGACCTCACGATGAAACCACAATCCGCGACACAGCGCACGGAAATCGCCAGc                                                                                                                                                               >  1:72361/1‑301 (MQ=255)
                                                                                                                                 gCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGTGTCATCAAGATGAAACAGCAATCGCTGACCCCGAGCACGTAAATCGCCAGcccc                                                                                                                                                            >  2:418132/1‑300 (MQ=255)
                                                                                                                                        ccccggagaGCCATCAACGACCAGCGGCCAAGGATGCAGCACCGAAGGCTGCATCTCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCAGGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTg                                                                                                                                                    <  1:8204/293‑1 (MQ=255)
                                                                                                                                                          gATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAGCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTGGCGATAGTTATCACTTCgc                                                                                                                                  >  2:173219/1‑301 (MQ=255)
                                                                                                                                                           aTCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGGCATCCAGGTTAAACAGCAATCGCTGACCCAGCGCCCGTAAATCCCCAACCCGGCTGTTTAGATAGTTATCACTTAgac                                                                                                                                 >  1:17218/1‑299 (MQ=255)
                                                                                                                                                                                 ccGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGACCGCCCTTTGAGATCGTTATCACTTAGCGCGGCAAACGGTTCGGCAAAttt                                                                                                           >  2:281585/1‑301 (MQ=255)
                                                                                                                                                                                                   cgcCCAACACGGCAGGGCTCCCCATGTCCCAGACACTGATCGCAGAAGGGGAGTTGGCTTGGCCATCTCGCACGACAACACCAACTAAGCGATCCTAGGGCATCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTAc                                                                                         <  1:418024/301‑1 (MQ=255)
                                                                                                                                                                                                        aTCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCCGACCCCGCGCACGTAAATCGCCAGCCCGCCCTTTGACATAGTTATCACTGAGCGCGGCAAAGCGTTCGGCCACATTTTCCATGACCGTTTTTTCCGccc                                                                                    >  1:67664/1‑301 (MQ=255)
                                                                                                                                                                                                             ggTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCCCTTTGAGATAGTTATCACTTAGCGCGGCACACTTTTCGGCAAATTTTTTACAGACCGTTTTTCCCGcccccgct                                                                               >  1:432607/1‑296 (MQ=255)
                                                                                                                                                                                                              gTAGGGTACCCCCGCGCACGTACCATGATCGCCGCATGGAGGTTGGCGTCGCCAGCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTg                                                                              <  2:446037/301‑1 (MQ=255)
                                                                                                                                                                                                                       cccACCGAACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGc                                                                     <  2:165720/301‑1 (MQ=255)
                                                                                                                                                                                                                         ccgagcacaaagcaagatgccagcagGGCGGTTGGCCGCCCCACCTCACACGACAACGCCGACGAAAGCAGCCGAGGCCAGCTCCGCAAGCGTTGGCGCTGACAGTTGATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCt                                                                   <  1:364199/281‑1 (MQ=255)
                                                                                                                                                                                                                           gcgcCCGTACCATGATCGAAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCt                                                                   <  2:454337/299‑1 (MQ=255)
                                                                                                                                                                                                                           gcgcACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCCCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCCCTCTGCCACCGAGCCta                                                                  >  1:454216/1‑299 (MQ=255)
                                                                                                                                                                                                                                       tGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCCGCGCCCGTCACTCGCCAGCCCGCTCTTTGCGATAGTTCTCACTTAGCTCTGCAAACTTTTCTGCACCTTTTTCACTGACCGTTTTTCCCGCCCCCTCTGCCACCGCGCCTTTATccccccccgc                                                     >  2:122526/1‑292 (MQ=255)
                                                                                                                                                                                                                                                 aTGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCCCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATAACCGTTTTTACCGCCCACTCTGCCACCACGCCTTTATCAACCTCGCCAAACAAtacc                                           >  1:323253/1‑298 (MQ=255)
                                                                                                                                                                                                                                                      ggTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACg                                      <  2:323345/300‑1 (MQ=255)
                                                                                                                                                                                                                                                              gcgcCATCTCGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCCCCGAGCCTTTATCAACCTCGGCAAACAATTCGCCACGCAGCCggg                              >  2:203069/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                      tcGCCCGACAACACCGACTAAGCGATCCCAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCAGCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCg                        <  1:194224/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tcGCACGACAACACCGACTAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCCACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCg                        >  2:194281/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                       cGCACGACAACACCGACGAAGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGaa                      <  2:371510/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                         cacaACAACACCGACTAAGCGATCCTCGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCAGCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGc                   <  2:432103/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         aaGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCTGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCCCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCATTGACCGTTTTTACCGCACACTCTCACCCGCATCCTTACTCAACCTCGGCAAACAATCCCCGACGCACCCggggctcccaagcacggggggctcaaaaa    >  1:139821/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                                         aaGCGATCCTAGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGGAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCCAGCCTTTATCAACCTCGGCAAACAAATTCCGCCGCCGCCGGGTGGCCGAAAGCAGGTGCGCGTAAAGa    >  1:339211/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                          aGCGATCCTAGGGCGGCTCAGCAAGCGTTGCCCCTTACGGTTCATCTGCCCCCAGAATGAAACGTTCAGGCCAGGCGTTCGGCCCTGGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGATc  <  1:122498/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                    
CTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCACCAGAATGAAACGTTCCGGCCAGGCGTTCGGCCCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAGCAGGTGCGAGTAAAGATC  >  NC_000913/2967126‑2967708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: