Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	4,208,389	0	A→G	100.0%	1.7	3	noncoding (243/1542 nt)	rrsE	16S ribosomal RNA of rrnE operon
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/0):  ref base (0/1):  total (2/1)

TCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT  >  NC_000913/4208089‑4208402                                                                                                                                                                                                                                                                                                             |              tctcAAAGGTGAACACGGAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTGTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGa               <  2:178968/301‑1 (MQ=11)   acAAGAGTTAACAACTAATTCATTGCGAAGTTTAATGCTTTGGTCAGTCAACGTGTAAATTTAAGAGTTTGATCATGGCTCAGAGTGAACGCTGGCGGGAGGCCTAACACATGAAAGTCTAACGGTCACAGGAAGCAGCTTGCTTCTTGGCTGACGAGTGGCGGACGGGTGATTAATGTCTGGGAAACTGCCTCATGGAGGGGGATAACTACTGGGAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGCGGACCTTCGGGCCTCATGCCATCGGATGTGCCCAGATGGGAtt             <  1:266952/300‑1 (MQ=11)             acacGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACAAATGCAAGACGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGAGGAGTGGCGGCCGGGTGAGTAATGTATGGGAAAATGCCTGATGGTGGTGGATAACTACTGGAAACGGTAGCTAATCACGCATACTGTCGCATGACCAGAGAGGGGGAACTTCAGCCCTCTTGCCATCGGATGTGGCCAGCTGGGGGTAGCTAGTAgg   >  1:398946/1‑301 (MQ=11)              cacGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTAGCAAGCACAAAGAGGGGGACATTCGGGCCACTTGCGATCGGATGTGCCCAGAGGGGGTTAGCtagagggg  >  2:616537/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                                             |              TCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT  >  NC_000913/4208089‑4208402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.