Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,289,190	0	A→C	100.0%	1.7	6	F298V (TTC→GTC)	insH1	IS5 transposase and trans‑activator
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (1/0):  total (6/0)

GTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACCCCTTGTATCTGGCTTTCACGAAGCCGAACTGTCGCTTGATGATGCGAAATG  >  NC_000913/2288890‑2289213                                                                                                                                                                                                                                                                                                             |                        gatGATGCCAATGCTATGGGTGAATATAAGGGCGGTGCGAATAAGAGGGGAAACTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGATTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGCTAGCCCCAGTTTTTAGTGAGATATCTCCCACTGACGTAAAATTTGGTACGCCCTCACGTGGTCGGTCAGACTGAATAACATCGCAAGAGGGGTATCGTTTTTCAGAAACCCCTTGTACATCACTGTGACAAAGCCGaa                         >  2:143388/3‑301 (MQ=14)            tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTTACGTATCATTTGCTCCGCCCGAAACAGGTTGGCCAGCGTGCATAACATCCCCAGTTGGTTATCGTTTTTCAGCACCCCCTTTTATTTGGCTTTCCCGACGCCCACCTGTCTCTtg               >  2:136190/1‑300 (MQ=11)            tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACCCCTTGTATCTGGCTTTCCCGAAGCCGACCTGGCGCTtgt              >  2:181528/1‑300 (MQ=21)             gCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACCCCTTTTTTCTTGCTTTCCCGAAGCCGACCTGTCGCTtgtt             >  2:7750/1‑299 (MQ=19)                   ggTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCCTTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTAAGCAACCCCTTGTATCTGGCTTTCACGCGGCCTAACTGTCGCTTTATGATGCg       >  2:146833/1‑301 (MQ=12)                       aaTATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGTTTCTTCGCCATTTACGGCGTTATCCCCCGTTTTTAGTGAGATCTCTCCCACTGCCGTATCCTTTGTTCCGCCCGAAACACGTTGGCCAGCGTGAATCCACTCGCCCGGTCGTGCTCGCTTTTCTGCACCCCCTTGTCTCTGCCTTCCACGCAGCCGACCTGTCGCtctctgttcccaaat   >  2:28416/1‑287 (MQ=11)                        atatAAGGAAGGTGCGAATAAGCGGGGAAAATCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCATTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCACAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACCGGTTGGCCATCGTGAATAACATCGCCTGTTGGTTATCGTTTTTCAGCAACCCCTTGTATCTGGCTTTCACGCAGCCGCCCTTTCGCTTGATGATGCGAAATg  >  1:160604/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                             |                        GTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACCCCTTGTATCTGGCTTTCACGAAGCCGAACTGTCGCTTGATGATGCGAAATG  >  NC_000913/2288890‑2289213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.