Read alignment evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_000913 | 3,997,531 | 1 | .→A | 1.6% | 1.3 0.0 ‑0.2 | 126 | coding (349/717 nt) | yigB | 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase; pyrimidine phosphatase; riboflavin synthesis |
Rejected: E-value exceeds prediction threshold. | ||||||||||
Rejected: Polymorphism does not pass frequency cutoff. | ||||||||||
Rejected: Polymorphism prediction not supported by minimum number of reads on both strands. | ||||||||||
Rejected: Polymorphic indel expands or contracts a homopolymer stretch. | ||||||||||
Reads supporting (aligned to +/- strand): new base (1/1): ref base (52/72): total (53/73) | ||||||||||
Fisher's exact test strand distribution p-value = 1.00e+00 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.50e-01 |
Only 100 of 129 total aligned reads displayed.
GGATGATACCCTTTACGATAACCGTCCGGTGATTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGA > NC_000913/3997233‑3997820 | ggATGATACCCTTTACGATAACCGTCCGGTGATTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCCGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑aa > 1:35354/1‑301 (MQ=255) ggATGATACCCTTTACGATAACCGTCCGGTGATTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCCCACGCAGCACGGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGCCCCGCAAC‑aa > 2:324189/1‑301 (MQ=255) cccTTTACGATAACCGTCCGGTGATTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGGTCGATTGAACAAGCGATGCCAGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATAAACGTTGACAAATGGCGCAGCCGACACGCCGTCCGCCAGC‑AAACCCACGa > 2:49271/1‑301 (MQ=255) cTTTACGATAACCGTCCGGTGATTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCCCGACGTGACGCGCTGGCGTTTTCGTTCGATGGAACAAGCGATGCTCGACGCCGGGCGGAGTCCCGAAGAGGCAGTTGCCGGCGCACACCCCGCCATGATGAACTTTGCCAACTGGCGCAGCCGCACAGGCGTCCGGCAGA‑AACCCCACgcc > 2:376509/1‑298 (MQ=255) ttcccctAACCGGCCCGTCATTGTCTGCATCGAGCGAGTGGAC‑CTTACCTCTCTGCAAAATTCTCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCTCGTCGGTACGGGAAGCGTAACCCGATATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGCTTTAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACGTTGCCAAATGTCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACAcc < 1:706160/295‑1 (MQ=255) ttAAGATAACCGTGCGGTGAGTTGGCGCCCCGAGCGAGAGGCG‑CTGACCGTCGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAATATCTGCAACGCCCGCGCCAGGCGGTACGGGAAGCGGAACCCTAGATTTGTCACGACGTGACGCGCCGGCGTTTTCGTTCGATTGAACACGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGCCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACAcc < 1:602224/301‑1 (MQ=255) tAACCGTCCGGTGATTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGAGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTGTAGTTCGCTTGCACCAGCGCTGCTCGACGCCGGGCTGAGTGCAGAAGAAGCCAGTGCAGGCGCAAACGCAGCAATAATCAACTTTTCACACAGGAGCAGCCGGAACGACGTACACCAGC‑AAAccaacacacacttacaa > 1:603425/1‑285 (MQ=255) tatcagaaaacgATTGTGCGCACCGAGC‑TCTGGCGCCTAACCCTTGGGCAAACTCATTATACTGCGCCGGCCAGCTTCCAGAAGGAAGATCTGCAACGGCTGCGCCAGGCGGTACGGGAATCGGAACCCTAGAGTAACCACGACGTGACGCGCGGGAGTTTCAGGTCGATGGAAGAAGCGATGCGCGCAGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTGTGCCAAAGGGCGCAGCCGAATCTACGTCCCGCAGC‑AAACTCACGACAACTTAAAAc < 2:560686/290‑1 (MQ=255) caCCGTCCGGTGATTTTTGTCACCGGGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCAGCCGGCGCTGCGCCGCGTCCCGAAGGAAGCTCGGCAACGCCTCCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTGTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAAc < 2:558690/300‑1 (MQ=255) aaCCGGCCGGTGAGTTTGCGGACCGAGCGGGAGGCG‑CGTAGCTTTGTGCAAAATTATCAGCCGGCGCTGGGGAGCTTCCAGAATGAAGATCGGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGGGACGCGCTGGCGGTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAAc < 2:586432/301‑1 (MQ=255) tcagaggggtataaggggcagccGAGCGGGAGGCG‑ATGACCTTTGTTGAAAATTGTCATGCGGCGCTGCGCAGCTTCGAGAATGAAGATCTGCAACGGCTGGGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTAGCACGACGGGACGCGCTTGCGTTTTCGTGCGATTGAACAAGCGATGCGCGACGCCGGGCTGAGTGCCGAAGAAGCCAGGGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAAc < 2:551528/279‑1 (MQ=255) ccGTCCGGTGATTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGCGATTTATCACGCCGTGACGCGCTGGCGTTTCCGCTCGCTTGAACAAGCGCTGCTCGACCCCGGGCGTAGTGCCTAAGAAGCCAGTGCAGGCGCCCACGCAACAATGCTCACCTTTTCCACATGGCGCACCGGAATCGCACTCCCTCAGC‑AAACCCACGACACCtcacacca > 2:34494/1‑293 (MQ=255) gTGATTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGCTGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCCCACGCAGCAATGATACACTTTGCCAAATGGCGCGGCCGAACCGACCTCCCGCAGC‑AAACTCACCACAACTTAAAACAACGGGCga > 2:682211/1‑301 (MQ=255) gTGATTTTGCGCACAGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATAATCCGGCGCTGCGCAGCTTCAAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAAAAAGCGATGCGCGACGCCGGGCTGAGTGCCGAAGAAGACAGTGCAGGAAAACACGCAGCAATGCACAACGTTGCCAACAGGCGCAGACGAATCGACGGCACGCAGA‑AAAATGACgcaacagtaaaaaggacggcgg > 1:646735/1‑280 (MQ=255) aTTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTACAAAATTATCATCAGGCGCTGCGCAGCTTCCAGAATGAAGCTATGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAAACCGAGATTTATCACGACGTGACGCGCTGGCTGTTTCGTTCGATTGAACAAGCGATGCTCGACGCCAGGCTGCGTGCCGAACAGGCCAGTGCAGGCGCACACGCAGCAATGCTCCACTTTGCCAAAGCGCCCAGCCGCGGCGACGTCCCGCAGC‑GGACTACCCACACCTTAACAcctgtcacttat > 1:560709/1‑289 (MQ=255) cACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCg > 1:273357/1‑301 (MQ=255) ccGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAACCGCCGTCCCGCAGC‑AAACCCACGCCACCGCAAAAcctctctcttatacacaactcc > 1:551551/1‑279 (MQ=255) gagcgagAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGACTGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGGGTGCCGCATAAGCCAGTCCAGGCGCACACCCAGCAATACTCAACTTTGCCAACTGGCGCAGCCAACTCACCGCCCCGCAGC‑AACCTCCCGCACCCTTACAACCGCTGGCGAAGAAACGGCCGCggg > 2:599025/1‑301 (MQ=255) agcgagAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACTTCCCACAGC‑AAACTCCCGACACCTTAAAACAGCGGGCCCAGAAATGCCCCCtggg > 2:706013/1‑300 (MQ=255) taggagcg‑tTTAACTTTGTACAAAAGTAGCAGCCGCGGCTGGGCAGGTTCCAGAAGGAAGATCGGAAACGCCGGTGCCAGGAGTGACGGGAAGCGGAACACGAGATTTATAACGGCGTGACGCTCTGGCGTTTTCGTACGAGTGAACAAGCGATGATCGACGCCGGGCTGATTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCtggtgg < 1:722145/295‑1 (MQ=255) atgcccttactgtgtcgCAAAATGATCATCCGGCGCTGGTCAGCTTCCACAATGCAGACCTGCAACGGCTGCGCCAGGAGGTACGGGAAGCGGAACCCGAGATTTACCACGACGTTACGCGCCGGCGTTTTCGTTCGATTGAACAAGCGATGCGCGACGCCGGGCTGAGCGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGACCAACTTTTCCAAATGGCCCAGCCGAATCGACGTCCAGCAGC‑AAACTCCCGACACCTTAAAACAGCTGCCGAAGAAATGGCCGCTGGTGGCTAt < 1:590867/285‑1 (MQ=255) gcg‑cTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCCGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCCCGACACCTTAAAACAGCTGGCGAAGAAATGGCCCCTGCTGGCGATc > 1:523349/1‑300 (MQ=255) gcg‑cTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCCCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACCCGCAGCAATGACCAACTTTGCCAAATGGCGCAGCCGCATCCACGTCCCGCAGA‑AAACCCACGCCACCTTAAAACACCTGGCGAAGAACTGGCCGATGGTGGCgctaa > 2:26019/1‑297 (MQ=255) gcg‑cTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGATTCCAGAATGAATATCTGAAACGCATGCGCCAGGCGGTACGGGAAGCGAAACCCGAGATATATCCCGACGTGACGCGCTGGCGGTTTCGTTCGATTGACCCAGCGAAACTCGCCGCCGGGCTGCGTGCCGACGCAGCCAGTGCAGGCGCCACCGCAGCAAAGATCACCTTTGCCAACTGGCGAAGCCGGATCGCCGTCCCGAAGC‑AAACTCACGAACCATTCAACCAGCTTGCGAAGCAATGGCCACTGGTGCCGATc > 1:116759/1‑300 (MQ=255) g‑cTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGACAAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACCGCTTGCGAAGAAATGGCCCCTGGTGGCGCTcca > 1:512478/1‑298 (MQ=255) ccTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAAAAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCCGCAATGATCACCTTTGCCAAATGGCGCCGCCGACTCGACGCCACGAAGC‑AAACTCACGACACCTTACCACAGCTGCACACGAAAGGGCCGCTGGTGGCGATccacagcgg > 1:743293/1‑293 (MQ=255) ccTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCGTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACgg < 1:595265/301‑1 (MQ=255) ccTTTGTGCAAAATTATCATCCGGCGCCGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTCCGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGCACAAGCGATGCTCGCCGCCGGGCTGAGTGCCGCAGAAGCCAGTGCAGGCGCACACGGAGCAATGATAAACTTTTCCACAGGGGGCAGCCGACACGGCGTCCCGCCGC‑AAAATCACACAACCGTGAAACAGAGGCCACAGGAATGGCCGcgcggggagcagccgcacgg > 2:47894/1‑282 (MQ=255) ccTTTGGTCAAAATTATCAGCCGGCGCGGCGCAGCTTCGAGAATGAAGATCGGCAACGCCTGGGCCAGGCGGTACGGGAAGCGGAACCCGGGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGGGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACgg < 1:82177/301‑1 (MQ=255) atgtgtgtcacaaTTTTCATCCCGCGCTGACCCGCGTCCACGAGGAAGCTCTTCAACGCCTGCGCCAGGCGGTACGGGAAGCTGAACCCGAGAGTTACCACGACGTGACGCGCTGGCGTTGTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACgg < 2:582495/290‑1 (MQ=255) gtgCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGACGCGGAACCCGAGCTTTATCCCAACGTGACGCGCTGGCGTGTTCGTTCGATTGAACAGGCGATGCCCGACACCGGGCTGAGTGCCTAAGACGCCTGTGCAGGCGCACACGCACCAATGCTCAACTTTCCCCACTGGCGCGGCCGAATCCACCCCCCCCACC‑CACCTCACCCCACCTTCAACCCACCGGCGCAGGCAGGACCGCTGGTGGCAACCCCCACCGGTACCg > 2:430139/1‑301 (MQ=255) gtgCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATTGCGCCGCCGAATCGACGTCCCGCAGC‑ACACTCACGACACCTTAAAACCGCTGGCGACGAAATCGCCGCCGGTTGCGAACACCacacgtaac > 1:193605/1‑292 (MQ=255) tgCAAAAATATCAGCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGc < 2:35352/301‑1 (MQ=255) gaaaaaTTATAATGCGGCTCGGAGCAGCTTCGAGAATGTAGATCTGCAACGCTTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTGATCACGACGTGACGCGCCGGCGTTTTCGTGCGATGGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGc < 1:34496/298‑1 (MQ=255) aTTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCCCCGC‑AAACTCACGACCCCCTCAACCAGCTGGCAAAGAAATGGCCGCTGGTGTCGCCCACCAACGCTAACGCCCacc > 2:709270/1‑298 (MQ=255) ccatgaccactttCCATGATTAATATCTCCAACGCCTGCCACAGGCGTTACGGGACTCGGAAACTGCGCTTTATCCCGACGTGACGCGCTGGCGTTTTCTTTGGATTGAACAAGCGATGCTCGACGCCGTGCTGAGTGCCGAAGAAGCCAGTGCAGGCTCACACGCAGCAATGATCAACTTTTCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCTAAGAAATGGCCGCTGGTGGCGATCTCCAACGGTAACGCCCAGCCGGAGCTgtttgg < 1:722201/290‑1 (MQ=255) cTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACCCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCCGGTGGCGCTCACCACCGGTAACCCCCCCCCGGAACTGTTTGGGTTgggggga > 2:638231/1‑299 (MQ=255) gAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGCGTGCCGATGAAGCCAGTGCCGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCAGCCGC‑ACACTCACGACCCCTTAAAACAGCAGGAGAAGAACTAGCCGCTGGTGGCGATCACCCAAACGAACCCCCCGCCGGAAGCGTTTGGTGTTGGGGGTTAtttt > 2:560064/1‑301 (MQ=255) gAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGCATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAAAAGCTGGCGAAGAACTGGCCGCTGGCTGCAATCCACAACGGTAACACCCCACCGGAGCCTTTTGCTTTgggggctgagttg > 2:110655/1‑293 (MQ=255) aaggaagttcgtcAACGCCTGGGCCAGGCGGGACGGGAAGCTGAACCCGAGGTTTATCCCGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGTCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTAttt < 2:205228/287‑1 (MQ=255) aacgAAGATTTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGACCCGGAGATGTAGGACGACGGGACGCGCTGGCGTTTTCGTTCGGTTGAACAAGCGGTGTTCGACGCCGGGCTGATTGCCGAAGAAGCCAGTGCAGGCGCACACGAAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTg < 1:489148/298‑1 (MQ=255) aaTGAAGGCCTGCAACGCTGGCGCCAGGGGGTACGGAGAGCGGAACCCGAGATTGATCACGACGTGACGCGCTGGCGTTGTCGGTCGATGTAACAAGCGACGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGGGCAGGCGCACACGAAGCAATGAGCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTAttt < 2:17971/299‑1 (MQ=255) aTGAAGCTCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATGTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACCAGCGATGCTCGAAGCCGGGCTGAGTGCCGAAGAAGCCAGTGCCGGCGCACACGCAGCAATGATCCACTGTGCCAAATGGCGCAGCCGACTCGACGTCCCGCAGC‑ACACTCACGACACCTTAAACGAGCTGGCAAAAAAATGGCCGCTGGTTGCGAACCCCACCGCTCCCGCCCAGCAGGACCGCATTGATTTGGAGAattatgataa > 1:424279/1‑296 (MQ=255) tgaatagcgtgaacgcgcgctccatccGGCACGGGAAGAGGAACCCGGATTTTAGCACGACGTGACGCGTCGGCGTTTCCGTTCGATGGAACAAGTGATGCTCGACGCCGGGCTGAGTTCCGAAGAAGTCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCATCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGTCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAg < 1:408993/275‑1 (MQ=255) tGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCGGGCGTTTTCGTTCGAGTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAAGGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAg < 1:599050/301‑1 (MQ=255) gacgtacggccagccttGCGCCGGGGGGTACGTGGAGATGAACCACGGAGTTATCACGTGGGGCTGCGCGGTCGTCTTCGTTCCATTGAACAAGCTATGCTCGCCGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCTCACCCGCAGCAAGGATCAACTTGGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCGGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAg < 2:646709/284‑1 (MQ=255) aaaGAGCTCCACCGCCGCCGCGAGGGTGGACGGGAAGCGGAACCCGAGATTTGTCACGACGTGACGGGCTGGCGTTTTCGTTCGATTGAACAAGAGAGGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGt < 2:304718/300‑1 (MQ=255) aaGATCTTCAATGCCTGCGCACGGCGGGACGTGAATAGGAACCCGAGATTTATCACGCCGGTACGCGCTGGCGTGTTCGTTCGAGTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAAGGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAg < 1:682237/299‑1 (MQ=255) gATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCCCGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTTGAGCTCACCAACGGTAACGCCCAGCCGGAGCTGGTGGGCTTGGGGGCTTATTTTGAGtttt > 2:115085/1‑300 (MQ=255) cTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGACTCGACGTCCCGCAGC‑AAACCCACGCCACCTTAAAACAGCTTGCTCAACAATGGCCGCTGGTGTCGACCACCCACGGTCACGCCCCGCCGGGGCTGTTTGGTTGGGGGGATTATTTTGAGttttgtt > 2:649129/1‑297 (MQ=255) cGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑CAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTACCGCCCAGCCGGCCCGGTTTGGTTTTGGGGATTATTTTGCGTTTGTG‑CTtcgcg > 2:551122/1‑301 (MQ=255) gcgcCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGCCGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTAGTGGCGATCACCACCGGTAACGCCCAGCCGGCGCCGTTTGGTTTGGGGGATTATTTTGACTTTTTG‑CTGCGCGCCgg > 1:74069/1‑300 (MQ=255) gcgcCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGCCGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCACCGGTAACGCCCAGCCGGAGCCGGTTGGTTTGGGGGATTATTTTGGGTTTGTG‑TTgcgcgcgtg > 2:643115/1‑297 (MQ=255) gCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGCAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAACCAGCTGGCGAAGAAATGGCCGCTGGGGCCGAGCACCAACGGTAACGCCCAGCCGGAGCGGTTGGGGTTGGGGGATTATTTTGAGTTGGGT‑CTGCGCGCGGGCCCGCCCg > 2:351758/1‑301 (MQ=255) ggtgaCGGGAAGCGGAACCCGGGATTTATCACGACGTGACGCGCTGGGGTTTGCGTTGCATTGAACAAGCGAGTCTCGACGCCGGGCTGGTTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACg < 2:310968/296‑1 (MQ=255) cGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGGTTGGGGGATTATTTTGAGTTTGTG‑CTGCGGGCTGGCCCGCACg > 1:412804/1‑301 (MQ=255) gTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGGTTGGTTTGGGGGCGTATTTTGAGTTTGTG‑CTGCGCGCTGGCGCGCACgcg > 1:631041/1‑298 (MQ=255) gTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGAGTGAACAAGCGATGCTCGACGCCGGGCTGAGCGCCGAAGAAGCCAGGGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACggg < 1:460473/300‑1 (MQ=255) gggAACCCGGGGTGTAGGAGGACGGGCCGCGGGGGCGTTTTCGTGCGAGTGAACAAGCGATGCGCGACGCCGGGCTGAGTGCCGAAGAAGCCAGCGCAGGCGCACACGCAGCAATGATCAACGTTGCCAAATGGCGCAGCCGAATCGACGGCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAAc < 2:434966/299‑1 (MQ=255) ctccggcgttttgcACGACTGGCCGCGCGTGCGTGTGCGTTCGATTGAACAAGCGATGCTCGACGCCGGGGTGAGTGCCGAAGAAGACAGTGGAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGGAGCCGAATCGACGGCCCGCAGC‑AAACGCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCGGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTc < 1:643141/288‑1 (MQ=255) acacgtgctatagcccgacgTTGCCCGCGGGCGGTTTCGTTCAAATGAACAAGCGATGCTCGACGCCGTGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGAAATGAGCAACTCTGCCAAATGGCGCAGCCGAATCTACGTCCCGCAGC‑AACATCACGACACCTTAAATCAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCCCCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTc < 1:560087/286‑1 (MQ=255) aCCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCCCGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGCTCACCAACGGTCACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCCCGGGCCCCCAAACCCggta > 2:507499/1‑298 (MQ=255) ccttggcgcacgactggcctcggtGGCGTTGGCGTTCAGCTGAACAAGCGATGCTGTACGCCGCGAGGCGGGCCGAAGAAGCCAGGGCAGGCGAAAACGCAGACAGGATCAACCTGGCCAAATGGCGCAGCCGAATCGACGGCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGAGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGa < 2:445451/278‑1 (MQ=255) cacgaggtgggcggcTGGGGTGTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCGGAGGGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTAc < 2:412793/288‑1 (MQ=255) acgacgTGACGCGCGGGCGTTTGCGTTCGATTGAAAAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCAAGTGCAGGCGCACACGCAGCAATGATTAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCTGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACtt < 1:638257/301‑1 (MQ=255) ccgTGACGCGCTGCCGTTTTCTTACGATGGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGGCCCGCAGC‑AAACTTACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACttttt < 1:351766/300‑1 (MQ=255) gTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAGTCGACGTCCCACAGC‑AAACGCACGACACCTTAAAACAGCTGGCGAAGAAATGCCAGATAGTTGCGAGCACCAACGGTAACGCCAGGCCGGAGCTGGTAGGTTGGGGGGATGATGTTG‑GGGTGTGTCTGCGAGCAGGACCGCACGGGCCGGCAAGACCTATAAGCGATATGTCATTTATgg > 1:263938/1‑301 (MQ=255) tgccgcgCTGGCGTGTTCGTTCGAGGGAAAAAGCGGTGCTCGACTCCTGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGACCAACTTTGCCAAAGGGCGCAGCCGAACCGACGTCCCGCAGC‑AAACTCACTACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCCCCAACTGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGc < 1:444260/298‑1 (MQ=255) ggCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAACTGGCCGCTGGTTGCGATCACCAACGGTACCGCCCAGCCGGAGCTGTTTGGTTTGGGGGGTTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCCCGGGCGCTCAAACCCGTTCCGCGTTCTTTCCTTTTTGGCTGGggcaca > 2:737780/1‑297 (MQ=255) tgttcggtctgttGAAGAAGCGATGCTAGAGGCCGGGCGGAGGGCCGAAGACGCGAGTGCAGGCGAAGACGCAGAAATGATCAACTGTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTGGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTc < 2:743266/290‑1 (MQ=255) gAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTCGGTTCGGTGGATTCTTTTTCGTTTGTG‑CTGCGCGCTGCCCCGCACGGCGGCTACAATCCGTTCAGCGTTCTGTACTTTCTGGCTTCGGCCACACTCAACGTTCCGATc > 2:8257/1‑300 (MQ=255) aGCGATGCTCGACGCCGGGCGGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGaaa < 1:66030/278‑1 (MQ=255) ctGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGTGCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCAGCAGC‑AAACGCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATc < 1:26021/299‑1 (MQ=255) cgccGCCGGGCTGAGGGCCGAAAAAGCCGGTGCAGGCCCCCACGCAGAAACGGTAAACGTTGCGAAAGGGCGCAGCCGAATCGACGTCCCGCAGA‑AAACTCACGACACCGTAAACCAGCTGGCGAAGAAATGGCCGCGGGGGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACAt < 2:381322/298‑1 (MQ=255) gccgacgggtgtagggCCGAAGAAGCGAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGA‑AAACTCACGACACCTTAAAACAGTTGGCGAAGAAATGGCCGCTGGTGGCGATCACTAACGGTAGCGCCCAGCCGGTGCTGTTTGGTTTGGGGGATTATTGTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGGTCAGCGATATGTACTTTTTGGCTGCGGAAAAACGCAACGTGCCGATCGGCGAGATCTTACAt < 1:192225/285‑1 (MQ=255) aCGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑ACACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCCACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTCTTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCCAAACCGATCGTCGATCTGTACTTTTTGGCCGCGGGAAAACTCGCAGTGCCGGTCGGCGAGATCTTAAATgg > 1:608775/1‑300 (MQ=255) aCGCCGGGATGAGTGCCGACGAAGCAAGGGCAGGCGCACACGCAGCAAGGATCAACGTTGCAAAATGGCGCAGCCGAATCGACGTCGCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCTCAGCCGGAGCGGTTTGGTTTGGGGGATTATTTTGAGTGTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATg < 2:193598/300‑1 (MQ=255) agtcgcGAAGAAGGCAGGGCAGGCGACAAGGAAGCAACGATCAACTTGACCAAAGGGCGAAGCCGAATCGACGTCCCGCAGC‑AAAGTAACGACACCTGAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGAt < 2:60226/295‑1 (MQ=255) tGCCGAAGAATCTAGTGCAGGCGAACCAGCAGAAATGACCAACTGTGCCAAATGGCGCATCCGAATCGCCTGCCCGCAGC‑AATCTCCCGACACCTTACAACAGCTGGCGAAGTAATGGCCGCTGGTGGCGCTCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTGGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGAtc < 1:644817/299‑1 (MQ=255) ggCCGAAGAACCCCGTGCAGGGTCACACGCAGCAATCATCAACTTTGACAAAGGGCGAAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGTAATGGCCGCTGGTGGCGATCACCTACGGTAACGGCCATCCGGAGCTGTTTGGTTTGGGGTATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGAtc < 1:706039/298‑1 (MQ=255) cGAATAAGGCAGTGCATGCGGACACGCAGCAATGAGCAAAGTGGCCAAATGGCGCAGCAGAATCGACGGCCCGCAGC‑AAACTCACGACACCCGAAAACAGCTGGCGAATAAATGGCCGTTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGAGTATTTTGAGTTTGTG‑CGGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGGGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTcacc < 1:430150/301‑1 (MQ=255) aagatgCCAGTGCAGGCACACACGCAACCATGATGCACGTGGCCAAAGGGCCCAGCCGAACTGCCGTCCCGCAGC‑AAACTCACGACACCTTAAAACACCTGGCGAAGAAATGGCCGCTGGGGGCGATCACCAACGGTACCGCCCAGCCGGAGCTGTTTGGTTTGTGGGATTATTGTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTcacc‑ac < 1:47897/301‑1 (MQ=255) aagaagCCAGTGCAGGCGCACACGCAGCAATGATCAACTTGGCCACATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGGTTGGTTTGGGGGATTATTTTGAGTGTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTcacc‑ac < 1:115090/301‑1 (MQ=255) ccAGTGCAGGCGCACACGGAGGAATGATCAACGTTGACAAAGGGCTGCGCCGAATCGACGTCCCGCAGC‑AAACTCGCGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACg < 1:738186/300‑1 (MQ=255) gCAGGCGCACACGCAGCAATGATAAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTGAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACgtgggt < 1:507522/300‑1 (MQ=255) cggacacGAAGGAAGGATCAACGCTACCAAAAGGGGGCTGCGAATGGACGTCCCGACGC‑AAACTCACGACGCCGTAAAACAGCTGGGGAAGAAATGGCCGCGGGTGGCGATCACCAACGGTAACGCCCAGCCGGTGCGGTTTGGTTTGGGGGATTATTTTGAGTGTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTGTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCGCACATGTTGGGGACGATCTCACC‑ACTGACGTGGGTGGGGc < 2:297263/297‑1 (MQ=255) cgcACACTGAGCAATGATCACCTTTGCCAAATGGCGCAGCCGAAGCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCGGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACGTGGGTGGGGc < 1:137578/300‑1 (MQ=255) cagcaATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAAACGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACGGGGGTGGGGCAATTCGCAg > 1:491887/1‑301 (MQ=255) cagcaATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCACAGCCGGATCTGTTTGGTTTGGGGGATTCTTTTGCGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCCAAACCGTTCAGCGATATGTACTTTTTGGATGCGGCAAAACTCAACGTCCCGATCGGCGAGATCTTACATGTTGGGTACTATCTCCCC‑ACTTGCGTTGGTGGGGCCATTCgaca > 2:205331/1‑298 (MQ=255) aaaTGAGCAACTGGTCCAAATGGCGCAGCGCAAGCGACGTCCCGCAGC‑AAACGCACGAGACCTTAAAACAGCGGGCGAAGAAATGGCCGCTGGTGGCTATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGTGGGATTATTTTGAGTGTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACGTGGGTGGGGCAATTCGCAGc < 1:224323/298‑1 (MQ=255) taCTTGGCCAAATGGCCGATGCGACTCGTCTGCCCTCAGC‑AAACGCACGGCAGCTGACGACAGCGGGCGAAGATATGGCCGCTGGGTGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTGTGGTTTCGTGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCGCGCACGGGCGCTAAAAACCGTTCAGCGATATGTACTGTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAgg < 2:263932/300‑1 (MQ=255) gCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTGTTTGGCTGCGGAAAAACTCAACGTGCCGGTCGGCGGGATCTTACATGTAGGGGGAGAGCTCACCAACTGAGTggggtgggagcatcacagcgggaagcaggcgcggt > 1:690643/1‑274 (MQ=255) gCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGGTCAGCGATCTGTACTTTTTGGCTGCGGCAAAACTCAACGTGCCGATCGGCGTGTTCTTCCCTTTTGGGGACGATCGCCCC‑ACTGCCGCGGGTGGGGCAATTCGCAGGGGCACGCAGGTttgtg > 1:299928/1‑300 (MQ=255) gcgcAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTGTTTTGAGTTTGTG‑CTGAGCGCTGGCACGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTAACC‑ACTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTgggacagg > 1:107244/1‑295 (MQ=255) gCCGAATTGACGTCCCGCAGC‑AAACGCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGGAACGCCCAGCCGGAGCTGGTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGTCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACa < 2:484520/226‑1 (MQ=255) gCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTGTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCACAACCGTTCCGCGATAACTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTAcg > 1:484535/1‑225 (MQ=255) gCCGAATCGACGCCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGc < 1:455077/187‑1 (MQ=255) cggagcGAGGTGCCGGAGC‑AAACGCACGACACCGTAAAACAGGGGGGGAAGGAATGGCCGCTGGGGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTGTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGaaa < 2:631016/296‑1 (MQ=255) cGAAACCACGTCCCGTAGA‑AAACGCACGACACCTCAAAACGTCGGGCGAAGGAAGGGCCGCTGGTGGGGGTAAACAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCGTGCCCGCACGGGCGCGCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGaaa < 2:273351/301‑1 (MQ=255) cGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGCTTATTTTGCGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGACCCGCGAGATCTTACATGTTGGGTCCGATCTCCCC‑ACTGACGTTGGTGGGGCACTTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAAGggg > 2:278778/1‑300 (MQ=255) | GGATGATACCCTTTACGATAACCGTCCGGTGATTTTGCGCACCGAGCGAGAGGCG‑CTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGC‑AAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTG‑CTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACC‑ACTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGA > NC_000913/3997233‑3997820 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |