Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,708,766	C→T	10.3%	V616V (GTC→GTT)	rsxC →	SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	1,708,766	0	C→T	10.3%	5.1 ‑0.4 ‑0.1	58	V616V (GTC→GTT)	rsxC	SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (29/20):  total (35/23)
Fisher's exact test strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.16e-01

CCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCG  >  NC_000913/1708472‑1709062                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                           cccGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCCGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCCACAGGCAAATGCCGGACCCGAAGAACCGGCCGATcc                                                                                                                                                                                                                                                                                                       >  1:186376/1‑300 (MQ=255) cccGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAAAAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCAGCGCAAAGCCGCCGGCGAAGCACGTCTGCCACGTGACAAAGCGCGAAAGCAGGAACCCCACCAGGCAACGGCGGAACCAGAAGCACAAGTCGaaaa                                                                                                                                                                                                                                                                                                       >  1:189173/1‑297 (MQ=255)    ggATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCCGAACAACAGGTCGACCCGCCCAAAGACGCCGGCGAAGCCGCGCTTACACGTCCCCAAGCCCGCCAGCTGCACCAGCCACAGGCTAATGCGGAAGCAGAACAACCGGCCGAGCcggc                                                                                                                                                                                                                                                                                                    >  2:332938/1‑298 (MQ=25)    ggATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTACTGCGGAGCCAGCAGAACAGGTTGATCcgcg                                                                                                                                                                                                                                                                                                    >  1:570847/1‑300 (MQ=255)    ggATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGTGCCAAAGCACACAATCGGGAAACGCCACAGGCTAAGAAGGAGCCAGAAGAACCGGTGGACCcggc                                                                                                                                                                                                                                                                                                    >  2:481930/1‑298 (MQ=25)         acaagggaatgatgggagcgCGGGAGGCCCGGAACGAGGAAACCGGAGCAAAACGGGCGAGACTGAAGGGAACGAAGGAGGCAGTAACGG‑GGGGTGAGACACGGAAAACGGC‑GTGAGAGGCAGCGAGCGAGTGGGCAAAAGCGCGGAAGCGGGAAGAGCAACAGGCTAATGCGGAACCAGAACAGCAGGTGGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgc                                                                                                                                                                                                                                                                                              <  2:648663/285‑1 (MQ=2)         aCAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGTAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGCTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACCGCCACAGGCTCCTTCGGAACCAGAAGCAAAGGTCGCACcgcgacaag                                                                                                                                                                                                                                                                                               >  1:520534/1‑295 (MQ=25)         aCAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAACTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCCCAAAGCCGCGATCCAAGCCACTATTGCCCGGGCCAAAGCGCGCAAGCCGCAACAGCAACAGGCAAAAACGGAACCAGACGAAAAGGCCGAACCACCCaaaaa                                                                                                                                                                                                                                                                                              >  2:707076/1‑299 (MQ=25)          ccaggtaagtagtGCAGAACGGGAAGACCGGAAAGCGCAAGCCAGGACGAAACAGGCAGAACTGCAGCAAACTAACGACGAAGCAACCG‑ATGCCAATCCACGTAAAACTGC‑CGTTGAAGCAGCAATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGATAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgcc                                                                                                                                                                                                                                                                                             <  1:246015/289‑1 (MQ=21)            gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACAAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCGACGCCGCCGTCGAAGCCGCTATTGCCCGTGCCACAGCGCGCCAACGGGAACACCAACAGGGGACTGCGGAACGAGAACAACAGGTCGCACCGCGACAAgccg                                                                                                                                                                                                                                                                                            >  1:584992/1‑300 (MQ=25)             ggaagtgagagacagcgaggAAGCACGGAAAGGGCAAGCAAGGCAGAAACAGGGAGAACTGCAGCAAACTAAGGACGCAGCAACCG‑GGGCTGACCGAGGTAAAACTGC‑CGTTGGAGCAGCTATCGCCCGCGCCGAAGCGAGCAAGCAGGAACAGCAACAGGCTACTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCaaa                                                                                                                                                                                                                                                                                                <  2:270611/277‑1 (MQ=18)              gCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGAAAGCTGGAACAGCAACCGGCTAATGCGGAACCAGAACAACCGGTCGATCCGCGCACACACGCCGTCGAAGCCGCCATTCCCCCGCCCACACCGCGCCAGCTCGAAACGCAACAGCCAAATGCGGCACCCGAAGAACCGCGCCATCCGCTCAAAgccacc                                                                                                                                                                                                                                                                                          >  1:273997/1‑300 (MQ=255)                 attattGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAACCTGC‑CGTTGCAGCAGCTATCGCACGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCAAATGCGGAACAAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCACTTGCACCTGCCACAGCGCACAAGCCAGACAGGCAACAGGCTAATTCGGGCCCCGAAGAACAGTTCGATCCACCCAAAgaggcacgcg                                                                                                                                                                                                                                                                                      >  1:306/1‑292 (MQ=255)                  ttattGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTCCCCAAGCCCTCAAGCTGGAACAGCAACCGCCTAATGCCGAACCAGCAGAACAGTTCGACCACAGCAAAGCCGCCGTcaa                                                                                                                                                                                                                                                                                     >  1:74100/1‑299 (MQ=21)                                tactccggaaaatacaaaccaagaccaaaaCAGTCAGAGACGCAACAAACAAACGAATCAGCACCCG‑TTTCTGATCCCACTAAACCTGC‑CTGTTACGCAGCCATCGCCAGCGCCAAAGCTCGGAAGCCGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAATCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGcc                                                                                                                                                                                                                                                                        <  1:741015/273‑1 (MQ=14)                                    cccGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAACAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCCATTGCCCGTGCCAAAGCGAGCAAACTGGAACAGCAACAGGCGAATGCCGAACCAGAAGAACAGGGAGATACGCGCGCAGCCGACGTCGACGACCCTATTGTCCgag                                                                                                                                                                                                                                                                    >  1:426309/1‑298 (MQ=21)                                     ccGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGCTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCAGGTCGATCCGCGCAAAGCCGCACGCGACGCCGCGATTGCCGGTGc                                                                                                                                                                                                                                                                   >  1:28412/1‑300 (MQ=255)                                       gTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCACCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGACCAGCAACAAGCTCATGCCGAACACAAAGAAAAGGGCGCTCCCAGCAAAGCCGCCGTCGCAGCGGCAATTCCCAGTGCCaa                                                                                                                                                                                                                                                                >  1:119529/1‑301 (MQ=21)                                            gcgcAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGCCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCCGCGCCAGAAGAACAGGTCGCACCGCGCAAAGCCGCCGACgcaccccacaaccacccgacgccaccga                                                                                                                                                                                                                                                           >  2:198947/1‑274 (MQ=18)                                                 aGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCCAAGCGCGCAAGCCGGAACCGCAACGAGCTAATGCGGAACCAGAAGCACACGTCTATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGGGCCAACGCACGCAAg                                                                                                                                                                                                                                                      >  1:411508/1‑301 (MQ=21)                                                      gagCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCCAAGCCGCCGTCGCAGCCGCTATTGCCCGTTCCAAAGCGCGCACGCTGGCACAGCAACAGGCTAATGCCGAACCAGAACAACAGTGGGATCCCCGCACAGCCGCCCCCTCACCCGCTCTTGCACGTCCCAAACCAccccaacggaa                                                                                                                                                                                                                                                 >  2:590059/1‑291 (MQ=18)                                                              aaGGCAGAACTGCAGCCAACTACCGACGCAGCAACCG‑TTCCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAc                                                                                                                                                                                                                                         <  1:669173/300‑1 (MQ=14)                                                                ggCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTCTTGCCCGTGCCACAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAAAACAGGATGATCCGCGCAAAGACGCCGTCGAAGCAGCTATTCCCCGCGCCaacagcagaacgatgacacagcaacag                                                                                                                                                                                                                                       >  1:213592/1‑276 (MQ=18)                                                                    gAACGGAAGCAAAACAAAGAGGAAGAAACAG‑TGGCGGAACCAGGAAAAACTGC‑CGGGGAAGCAGCCAGCGACCGCGACAAAGCGCGCAAACGGGAAAAGCAACAGGGTAAGGCGGAACCAGAAGAACAGGCCGATCCGGGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGc                                                                                                                                                                                                                                     <  2:570824/299‑1 (MQ=11)                                                                        accagcaAACTAACAAGCCAGCAAACG‑GTGCTGCACCCCGTAAAATTGC‑CTCGGAAGCAGCTAGTGCCGGCACCAACACTCCAAAGCTGGAAAAGCAACAGCCTAATGCGGAACCAGAACCACAGGTCGATCCCCGCAATGCCGCCGTCGAAGCCGCTATGGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc                                                                                                                                                                                                                               <  1:332944/299‑1 (MQ=17)                                                                         gcagcaAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCCAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCCCCAAGCCGCCGTCGAAGCCGCTATTCCCCGTGCCAACGCACGCCAGATGGCACAGCAACCGGATAAtcc                                                                                                                                                                                                                               >  1:642242/1‑298 (MQ=18)                                                                          cagcaAACTACAGACGCAGACACAG‑TTGTTGATCCCAGTAAAACTGC‑CGTTGAAGCAGCTATCGCACGTGAAAAAGCGCGAAAGCTGGAAAAGCAACAGGATACTGCCGACACAGACGCACAGGTCGATCCTCGCAAAGCCGCCGTCGAATCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAAGGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc                                                                                                                                                                                                                               <  1:733487/299‑1 (MQ=17)                                                                              aaaCTAACCACGCAG‑AACCGTTTGCTGTGTCAGCTAAAAGTGCGCGTT‑AAGAAGCTATGTCCGGCGCAAAAGCGGCAAAGCTGGAAAGTAAACAGGTACATGAGGAACCAGTACCACGGGTGGAGCGGCGGAAAACCGCCTTATAAGCCGGGATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAGCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGcc                                                                                                                                                                                                                        <  1:751802/301‑1 (MQ=9)                                                                                    aacggcgcagacacgttgtcggagcaaacaaaaaCAGC‑GGTTGAAGCAGCCGTCGACGAGGCCAGAGCGCGGAAGCTGGAACAGAAGCAGGCGAAAGCCGAAGCAGAGGTGAAGGTGGATCCGCGCAAGGACGGCGTCGAAGCAGCTATTGCCCGTGCAAAAGCGCGGAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGaac                                                                                                                                                                                                                   <  2:273991/271‑1 (MQ=14)                                                                                        ccgagcaACCG‑TTGCTGACGCCCGGAAAACGGC‑CGGGGAAGCAGCGATCGGCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAg                                                                                                                                                                                                              <  1:590084/298‑1 (MQ=17)                                                                                         gcagcaACCG‑TTGCTGAACCACGAAAAACTGC‑CGTGGAAGCAGCTAGCACCCGCGCCAAAGCGAGCAAGCTGGAACAGCAAAAGGCTAATGCGGAACCAGAACAGCCGGTCAAGCCGCGCAAAGCCGCCGTATAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACTGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAATGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATTC‑CGAGCCAGAACAACAg                                                                                                                                                                                                              <  1:14629/300‑1 (MQ=17)                                                                                                    ttGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACATGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCCAAGACGCAATTGACCGTCCCAAACCACACAACCTGGAACAGCAACAGGCCAATACGCGA‑CCAGAACAAAAG‑CTCCATCcgc                                                                                                                                                                                                   >  1:345137/1‑300 (MQ=17)                                                                                                    ttGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCCATTCCCCGCGCCAAGGCACGCAAGCTGGACCAGCAACAGGCTAATGC‑GGAGCCACAAGAACAG‑TTAGATCcgcg                                                                                                                                                                                                  >  1:206297/1‑301 (MQ=17)                                                                                                      agtgagccaaagaaaaCTGG‑CGTAAGAGCAGATAAGGCCCGGGACGAAGCGGGCAAGACGGAACAGCAACAGGATAATGCGGAACCAGAAAAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCAGCTATGGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCgcgc                                                                                                                                                                                                 <  1:153512/288‑1 (MQ=14)                                                                                                              aCGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGCACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAGCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAACTGGAACAGCCACCAGCTAATGC‑CGAGCCCGaaacaaaggtagagacgcggcaccccgc                                                                                                                                                                                          >  1:239215/1‑274 (MQ=14)                                                                                                                      cgtgacagtcatgaagcTATCGCCCGCGCAGAAGCGCGAAAGAGGGAACAGCAACAGGATAATGCCGAAGCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCGCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                 <  1:254196/287‑1 (MQ=14)                                                                                                                                                            tcacccggAAGAGCAACAGGACAATACGGAACCGAACCCACCAGACGATCGGCGCAAACACCCCGTACAAGCCGTGATTGACCCCGCCAAAGCACTGCAGCTGAAACCGCAACAAGCTAAGGCTGAACCAGAATACCTGGTCGATACAGGAAAAGCCGCCGTCGAAGCAGCTATGGCCCGTTCCTAAGGAAGAAAACTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATACGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGTCAAAGCGCGCAAACGGGAACAg                                                                                                                                           <  1:528892/295‑1 (MQ=9)                                                                                                                                                                        gCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCCACCTGGACCAGCCACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCCAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAACATGGAACAGCCACAGGCTAATTC‑CGAACCAGAAGAACAGAGTCACCCCGC‑CAACGACGCCCACCAAGCCACTATTGACCCACCCAAAGCCCCCACACgccaccacgacaccgctca                                                                                                                                >  2:355687/1‑282 (MQ=14)                                                                                                                                                                              ggCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGCACAGCAACAGGCTAATGC‑CGAACCCGCACAACCG‑GTCGCTCCGCCCAAAGCCGCCGCGCAAACCGCTCCTTCCGCAGCCCAAACCACCCAACGGGAACAGCCACCGGccactcccgcg                                                                                                                         >  1:202667/1‑291 (MQ=14)                                                                                                                                                                                      ctgcaccccacctacaGGTGGATCCCCGTAATGCGGCCGCTGAACCAGCTAGTCCGCTCGCCCAAGCTCGCAAGCTGGAACCGCAACATGCCAATGCGGAACCAGAACAGCAGGTCGATCCGCTCACAGCCACCGTCGAATCCGCTATTCCCCGCGCCACAGCACGCAAGCTGGAACAGCAACATGCTAATGC‑CGACCCAGCACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaag                                                                                                                  <  1:750999/287‑1 (MQ=11)                                                                                                                                                                                        aacgcagcacaaacggtcagtgcccgaaaaGCCCCCAGCGAACCCGCTATTACCGGTGCCAAAGAGCGTAAGCGGGAACCGCAACAGGCTAATGCGGAACCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGTTAATGC‑CGATCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                 <  1:158257/272‑1 (MQ=14)                                                                                                                                                                                            cAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAg‑g                                                                                                                                                                                                            >  2:696211/1‑204 (MQ=255)                                                                                                                                                                                              gAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCCAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGC‑CGAGACAGAACAACAG‑GTCGATCCGGGCACAGACTCACACGCACACGCACTAGACCGATCCAAAGCGCGAATAAGTGCAGATCAACCGACACATGCGGAGACCCAAGGACAggaag                                                                                                         >  1:631506/1‑298 (MQ=14)                                                                                                                                                                                                                cgaaaaGCCGGCGTCGAAGCGGCGATAGCAGGCGCAAAAGCACGGAAGCAGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAGTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCg                                                                                       <  1:198954/298‑1 (MQ=14)                                                                                                                                                                                                                               aaggagctcggagcgggtacaaaaGCG‑GCAAGATGGCAGAGCAACAGTCTAATGCCGACCCAGAAGGAGCGGTCGATCGGCTCAAACCCGTGGCCTAAGCCGCTATTTCCCGCGCAAAAGCCCGCAAGCTGGAACATCAACAGGATAATGC‑CGAGCCAGAACAACCG‑GCCCATCCGCGAAAAGCCGCCGTCGAAGTCGCTATTGCCCTAGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGATCCAGAAGAACAGGTTGCTCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg                                                                        <  1:228004/279‑1 (MQ=255)                                                                                                                                                                                                                                    cGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAACGCCGCCGTCGAAGCGGCTATTGCCCGGACCCAAGCCCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGCGCCGCGCAACGCTGccctacaccacgctattgaccg                                                                    >  1:39142/1‑280 (MQ=14)                                                                                                                                                                                                                                            cccATGCCACAACGCGCAAGCCTGCAAGGGAACAGCTTAATGCGGAACAAGCACAACAGGTCGAGCCGCGCAAAGCCGCCGTCGAAGGCGCCATTACCCGTGCCAAAACACTAAAGCTGTAACAGCAACAGGCTAATGC‑GGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGc                                                           <  1:97027/301‑1 (MQ=14)                                                                                                                                                                                                                                            cccATGCCAAGGCGACCAAGACGGAACAGCACCAGTCAAGTGCGGAACCAGCAACAAGGGGCGATCAGCGCAACGCCGCATGGTACGCCGCCACTGCCCGGGCAAAAGACAGCAAGCTGGAACCGCAACAGGTCAATGC‑CGAGCCAGAACAAAAG‑GGCGAGCCGAGCAAAGCCGCAGTCGAAGCCGCTATGGCCCGAGCCAAAGCGCGAAAACGGGAATAGCAACAGGCGAATGCGGAGCAAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCGATTGCACGCGCCAAAGc                                                           <  1:157251/301‑1 (MQ=255)                                                                                                                                                                                                                                                tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAGAGCCGCCGTCGAAGCCGCTATTGGCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGATAATGC‑CGAGCCAGAACAACAG‑ATCGATGAGCGCAACGCCGCCGTCGAAGCCGCTATTACCCGAGCCAACGCGCGCAAACGGGAGCACAAACCGGATGATACGGAGCCCGAGGAGCAGGTGTAGCCGCGCAACGCTGCAGTCGCAGCGGCTATTCCCGGCGCCAGAGCACg                                                        >  1:670173/1‑300 (MQ=14)                                                                                                                                                                                                                                                                        gCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAACGCCGCCGTCGAAGCGGCTCTTACCCGCGCCCCAGCCCGCAAACCGGCACAGCAACCGGCTAATCCGGGGCCAGAAGAACAGGTTGAACCACGCAAAGCGCCCCTCGAGACGGCTATTACCCGCCCCCAACAACGCAACACGGAGCACAAACAGGCtgaa                               >  2:101101/1‑298 (MQ=14)                                                                                                                                                                                                                                                                              ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGAACCAAAGCGCGCAAAAGGGAACAGCAACAGGCTAAAGCGGAGGCGGAGGAACAGTTCGGACCGCGCAAAGCTGCCATCGCAGAGGCGATTGCCCGGCCCACAGGACGCAAGACGGCACACCAGCAgacaaagtgcgta                         >  1:671924/1‑289 (MQ=14)                                                                                                                                                                                                                                                                              ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGc                                                                                                                                          >  1:22505/1‑188 (MQ=255)                                                                                                                                                                                                                                                                                 caagactgAACCAAAAGATCCCGTCTATGCTGGCAACGCCGGCGTCCAACTCGCCATGCCCCGTGCCAAAGCACACAAGCTGGCACAGCAACAGGCTAATGC‑TGACCCAGAACACCAG‑GTCGACCCGCGCAAATCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCa                      <  1:262591/294‑1 (MQ=14)                                                                                                                                                                                                                                                                                         aaCCACAAGAACCGGCTGATCCGGCCACGGCCGCCGTCGAGGCCGCCAGTGCCCGTGAAAAAGCGCGCAAGCTGAAACAGCAAAAGGCAAATGC‑CGCGCCAGAACAACAG‑GACGACCCGTGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAAc               <  1:432892/300‑1 (MQ=17)                                                                                                                                                                                                                                                                                                 gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGGACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCCAAAGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCAGCGCAAAGCTGCCGTCGAAGCGGCTCTTGCACGCGCCAAAGCAAGACAACAGGCACCGCAACAGGCCAAtcagacacgagacacaacggttgca       >  1:325288/1‑276 (MQ=14)                                                                                                                                                                                                                                                                                                 gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAACCGGGAACAGCAAACGGCTCATGCGGCGCCCGCACGACAGGTTGATCCACCGAAAGCTGCCGTCGAAACGTCTCTTGACCGCGCACAAGCACAAAAGATGGAACCGCAACAGGCTAGTCCGATGCCACAACAACAgataact       >  1:662189/1‑294 (MQ=14)                                                                                                                                                                                                                                                                                                     aGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCCGAGACAGAAGAACAGGTTGAACCGCGCAACGCCACCGTAGAAGCGACTCTTGCACGCGCCAAGGCCCGCAAACTGGACCACCAACAGGCAGATCCGGTGCCACAAACACAggacgcaccac   >  2:625456/1‑291 (MQ=14)                                                                                                                                                                                                                                                                                                      ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCACCAGGCTAATGCGGTACCCGAAGAACAGGTTGATCcgcg  >  1:457238/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                           CCCGGATAACAGTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCG‑TTGCTGATCCACGTAAAACTGC‑CGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGC‑CGAGCCAGAACAACAG‑GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCG  >  NC_000913/1708472‑1709062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.