| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,187,507 | A→C | 7.2% | N720H (AAC→CAC) | rpoC → | RNA polymerase, beta prime subunit |
| Read alignment evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | change | freq | score | reads | annotation | genes | product | ||
| * | NC_000913 | 4,187,507 | 0 | A→C | 7.2% | 11.6 ‑0.3 ‑0.0 | 114 | N720H (AAC→CAC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): new base (6/4): ref base (53/50): total (60/54) | ||||||||||
| Fisher's exact test strand distribution p-value = 7.45e-01 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.17e-01 | ||||||||||
Only 100 of 115 total aligned reads displayed.
TGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGG > NC_000913/4187209‑4187805 | ttggggccatttcactttccccgtctgtgCCTATGAGGCTGGTTCTGGTGTATCTGTTAGTATTGATTACAGTGTTAACCCGGAGAAAGAACACGAAAGCAGCTCCGAAGAAAAAGCAGAAGATGCTGAAATTGAGGAGCAGTTCCAGTTTGGTCTGGTAACTGCGGGCGATCGCTACAACAAAGTTATCGATATCTGGGCTGCGACAAACGATCGTGTATCCAAAGCGATGATGGATAACCTTCAAACTGAAAGCGTGATTAACCGTGACGGTCAGGAAGAGAAGCGGGTTTCCTTCAAc < 2:144714/273‑1 (MQ=255) gACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGCTGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGAAGGTTTCCTTAAACAGCa > 1:662483/1‑301 (MQ=255) aaggtggttataagagacagcGCCTATGCATCGCGTTCGGGGGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCt < 2:734606/281‑1 (MQ=255) gATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGCTGGATAACCTGCCAACTGAAACCGCGATTAACCGTACCGGTCAGGAAGAGAATCAGGTTTCCTTCAACAGCacccaa > 2:689143/1‑296 (MQ=255) aTCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTCCa > 1:452994/1‑301 (MQ=255) cATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGAGCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGACACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTAAACAGCATCTACatg > 1:553348/1‑301 (MQ=255) tggctgatgcTAGGCAGCGCGTTCTGGTGCAGATTTTGGTATCGATGACATGGGCAGCCCGGAGAAGAAAGACGAAATCGTCTCCGAGGCAGAAGCAGAAGTTGATGAAATTCAGGAGCAGTTCCAGTCTGGTCGGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGAGATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATGAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGAc < 2:272165/292‑1 (MQ=255) ggctgctacgatggctggcgTTCGGGGGCATATTGTGGGATCGATGAAATGGGCATCACGGAGAAGAAACACGAAAGCATCGCCGAGGCAGAAGCAGAAGTGGCTGAAATGCAGGAGCAGTGCCAGTCTGGGCTGGTAACTGCGGGCTAACGCTACAACAAAGGTATCGATAGCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGGTATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGTCGAc < 2:498201/283‑1 (MQ=255) cttagttcaagCAGCTCGTTCGGGTTAATCGGGTGTTATGAATGAAAAGGGCAGCCTGGAGAAGAAACACGAAATCTGCTCCGAGGAAGAAGCAGAAGTTGCGGAAATTCAGGAGGAGGTCCAGTCTTGTCTGGTAACTGCGGGCGAACGCGACAACAAAGTTATCGAGATCTGGGATGCGGCGAACGATCGGGTATCCAAAGCGATGATGGATAACCTGCACACTGAAAACGTGATTAACCGGGACGGTTAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTc < 2:736490/290‑1 (MQ=255) tCGCCTAGTCCGCGCGTTCTGGGGCATCTGTTGGTATCGATGAAATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCgg < 2:677435/301‑1 (MQ=255) cGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGACGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGACCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGACAGCTACAACAACGTTATCGATATCTGGGCTGCTGCGAACGATCGTGTCTCCAAAGCGATGCTGGAGAAGCTGCACACTGAACACGTGATTAACCGTGACGTACAGGAAGAAAAGGCGGTTTCCTTCACCAGCATCTACatactgccacaccccgg > 2:749511/1‑285 (MQ=255) cGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGACATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTCTCGCTATCTGGGCTGCGGCGCACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCACGAAGAGAAGAAGGTTTCCTTCAACAGCATCTACCTCATGCCCGACTCCgc > 2:83174/1‑299 (MQ=255) cGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACACAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCACACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCgg > 2:337092/1‑300 (MQ=255) cGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGCTTTCCTTCAACAGCATCtctgtctcttatcaacatct > 1:734633/1‑281 (MQ=255) cGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGCCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGACGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGt > 2:547200/1‑301 (MQ=255) aTGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACCCGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGACATTCAGTAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGCACGCTACAACACAGTTATCGATATCTGGGCTGCGGCGAACGAACGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAGCCGTCACGGTCCGGAAGAGAGGCAGGGTTCCTTACACAGACTGTACATGATGGACgcagccggggggcg > 2:377018/1‑288 (MQ=255) gTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGAGCTGCGGCGAACGATCGTGTATCCAACGCGATGATGGATAACCTGCCAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGACGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGGGGTTCTgg > 2:66789/1‑300 (MQ=255) tCTGGTGCAGATGTTGTGTTCGATGACATGGTCATCCCGGAGAAAAAACACGAAAGCATTGCCGAGGCAGAAGCAGAAGTTGCGGAAATTCAGGAGCAGTGCCAGTCGGGTCTGGTAACTGCGGGCGAACGCTACAGCAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTAGCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCgg < 1:329937/301‑1 (MQ=255) gTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACCAAGTTATCGATCTCTGGGCTGCGGCGAACGATCGTGTATCCAACGCGATGATGGATAACCTACAAACTGCACCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCATTCACAGGCCTCTACCTTATGGCCGACCCCGGAGCGCGTGGTTCTGCGGcgca > 2:80946/1‑301 (MQ=255) cTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGCAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGCCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCg > 2:93699/1‑301 (MQ=255) tGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCAGACTCCGGTGGGCGTGGTTATGCGGCACAGATTCGt > 1:290435/1‑301 (MQ=255) gTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGCTATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGCAACCGTGATTACCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTAAACCGCATCTACATTATGGCCGACTCAGATTCGCGTGGTTTTGCGGCCCAGCTTctta > 2:331532/1‑298 (MQ=255) gTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGCCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAg > 1:37897/1‑299 (MQ=255) cGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGACGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGACACCGTGATTAACCGTGACGGTCAGGAAGAGCAGCAGGTTTCCTTCAACAGCATCTACCTGATGGCCGACTCCGGTGCACGTGGTTCTGACGCCCATATTCATCAACTTg > 1:698942/1‑299 (MQ=255) agACATGGTCCACCGCGAGAAGAAACACGGAATAATCTGCGAGGCGGATGATGCAGTTGCTGAAATTCAGGAGCAATTCCAGTGTGGTTTGGTAACGGCGGGCGAACGCTACAACAAAGTTATCGATAGCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCTTCAGCTTGCTgg < 2:605945/299‑1 (MQ=255) aTGGTCATCCCTGAGAAGAAACACGAGATCATCTCCGAGGCAGAAGCCAAAGTTGCTGAAATGCAGTATCATGCCCAGTCTGGTCGGGTAACTGCGGGCGAACGCTACAACACAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGCTGGATAACCGGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGc < 1:97115/301‑1 (MQ=255) ggtataccgggAGAAGAAACAAGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCGGAAAGTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCg < 2:452980/291‑1 (MQ=255) ggTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGCTTAACCGTGACGGTCAGGAAGAGAAGCAGGGTTCCTTCAACAGCATCTACATGATGGCCGCCTCCGGTGCGGGTGGTTCTGCGGCCACGATGCGTCGGCTTGCTGGTATTCgg > 2:262895/1‑300 (MQ=255) gTCATCACGGAGAAGAAACACGAAATCATCTCCAAGGCGGCAGCAGCAGTTGCTGACAGTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGAGATCTGGGCTGCGGCGTACGATCGTGTATCCAACGAGATGATGGATAACCTGCAAACTGAAACAGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGCTGGACGACTCCGGTGCGCGTGGTTCTGCGGCACAGACTCGTCCGCTTTCTGTTTTGCGTg > 2:178640/1‑301 (MQ=255) tCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAAc > 2:628054/1‑224 (MQ=255) tCCCGGAGAAGAAACACGAAAGCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAAc < 1:628079/224‑1 (MQ=255) agaaggAACACGAAATCGAATCCGAGGCAGAAGCAGAAGTTGCTGAAAGTCAGGAGCAGTTCCAGTCGGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTGATCGATATCGGGGCGGCGGCGAACGATCGTGTATCCAAAGCGATGAGGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAAGAAGAGAAGCAGGTGTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATgg < 1:83178/300‑1 (MQ=255) agaagaACCACGAAATAATCTCCGAGGCAGACGCCGAAGTTGCTGAAATTCAGGAGCAGTTCCAGGCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATgg < 1:99464/300‑1 (MQ=255) agaagaAACACGAAATCAGCTCCGAGGCAGAAGCAGAAGGTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCGGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATgg < 2:451725/300‑1 (MQ=255) aaaagaaACACGAAAGCATCTCCGAGGCAGAATCATGAGTTGCCGAAATTTAGGAGCAGTGCCAGTGTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCGGGGATGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATgg < 1:146670/298‑1 (MQ=255) aaTCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGCAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATgg > 1:600343/1‑300 (MQ=255) aaTCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCGGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCACACTGACACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCATGTTTCCTGCCCCAGCATCTACATTATGGCCGACGCCGGTGCGCGTGGTTCCGCGGCCCAGATTCGTCAGCTTGCGTGTATGCGTGGTCTTGTGGCGACACCGGAGggg > 2:572669/1‑300 (MQ=255) tcatagccGAGGGAGAAGCAGAAGGTGCTGAAAGTCAGGAGCAGTTCCAGTCTGGTCTGGGAACTGCGGGCGAACGCTACAACAAAGTTATCGAGATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTc < 2:600318/295‑1 (MQ=255) tctcCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATTATGGCCGCCTCCGGTGCGCGTGGTTCTGCGGCCCAGATTCGTCAGCTTGCTGGTATTCGTGGTCTGATGGCGAAGCCGGCTGGCTCcat > 2:58392/1‑301 (MQ=255) ccggAGGCAGAAGCAGAAGTTGATGAAATTCAGGAGCAGTTCCAGGCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTGCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCcatca < 1:93703/298‑1 (MQ=255) agtgcatgaatatCAGGTGCGGTGCCAGTCTGTGCTGGTAACTGCCGGCGAACGATACAACAAAGTGACCGCTATCTGGCCTGCGGCGAATGATCGTGTACACAAAGCGATTAGGGACACCCTGCAAACTGAATCCGTGATCAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCAc < 1:487153/288‑1 (MQ=255) cTGAAAGTCAGCAGCAGTTCCAGGCTGGGCTGGTAACTGCGGGCGAACGATACAATAAAGTGATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGa < 1:377029/300‑1 (MQ=255) aTTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGCATGCGTGGTCTGCTGGCGCAGCCGGATGGCTCCCTCATCGAACCGCCAATCCCCCCGAACTTc > 2:83112/1‑300 (MQ=255) cAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACC‑‑GACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTc > 2:321743/1‑204 (MQ=255) ggacaggtgcAGGCTGGTCTGGGAACCGAGGGCGAAGGCTAAAACAAAGGTAACGAGATGTGGGGGGAGGCGAACGATCGTGTATACAAAGCGATGATGGATAACCTGCAAACCGAAACAGTGATTAACCGGGAGTGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTATGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGa < 1:451930/290‑1 (MQ=255) gatcAGGTCCAGTCGGGTCTGGTAACGGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCGGCGTCGAACGAGCGTGTATCAAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGCTGAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGa < 1:666255/296‑1 (MQ=255) tttCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAACCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAgg < 1:591885/296‑1 (MQ=255) gTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGATCAATCATCTAAACGCCAATCACCGCGAACTTCCGTGAAGGt > 2:521109/1‑298 (MQ=255) gTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAAAGATCGTGTCTCAAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGCAGCAGGTTTCCTTCACCAGCCTCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGCTGGCTCCATCATCGCAACGCCCATCAACGCGAACTTCCGTGCAGGt > 1:558235/1‑298 (MQ=255) tacgtcgtggcgtggaatcgCGGTCGAACTGTATAACAAATTTATCGATATCTGGGCTGCGGCGAACGTTCGTGTATCCAAAGCGATGATGGATAACCTGCAAAAGGAAACCGTGAGTAACCGGGACGGTCAAGAAGAGAAGCCGGTTTCCTTCACCAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAAc < 2:319423/282‑1 (MQ=255) tagtgtgggttggaaaggtcGGGGGAAGGCGACAAAAAAGTTAGCGATATCTGGGCTGCGGGGAACGGTCGTGTATCCAAAGCGAGGATGGATAACCGGCAAGCGGAAACCGTGAGTAACCGTGACGGTCAGGAAGAGGAGCAGGTTTCCGTCAACAGCAACTACATTATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCAGCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACg < 2:716944/282‑1 (MQ=255) ttggtctggtAACTGCGGGGGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGACCGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACt < 1:521132/300‑1 (MQ=255) tgtgtttggtAACTGCGGGCGAAAGCTACAAAAAAGTTGTCGACATCTGGGCTGCGGCTAACGATCGTGTATCCAAAGCGATGATGGATAACCGGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCGACATGATGGCCGACTCCGGTGCGCGTGGGTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACt < 1:564721/295‑1 (MQ=255) ctggtctggtAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTGCCGGGAAGGTCTGAACGTa > 1:407247/1‑299 (MQ=255) gtctggtAACTGCGGGCGCACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATAGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTAAATGATGGCCGACTCCGGtc > 1:513676/1‑176 (MQ=255) gtctggtAACTGCGGGCGAACGCGACAACAAAGGTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGAGGGATAACCTGCAAACGGAAACCGTGATGAACCGTGACGGGCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTg < 2:513653/177‑1 (MQ=255) tgtgtaaaaaagaacgcGCTACAAAAAAGTGATGGATATCGTGTCGGCGGCGAACTATCTTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGAGTAACCGGGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACAGGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTAc < 2:620891/285‑1 (MQ=255) gtAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTAc < 1:58396/300‑1 (MQ=255) gtAACTGCGGGCGAACGATACAACAAAGTTATCGATATCTGGTCGGCAGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACGGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGGATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTAc < 1:80950/300‑1 (MQ=255) ggaaCTGCGTGCGAAGGTGAAAAAAAAGTTATGTCTAGCTGGGCGTCGGCGAACGATCTCGTATCCAAAGCGATGATGGATAAACTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTAc < 2:580626/298‑1 (MQ=255) cTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACatgat > 2:179612/1‑154 (MQ=255) ggcggCGAACGCTCAAACAAAGTTATCGATATATGGGCGGCGGGCAACGATCTTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCAtc < 2:290429/297‑1 (MQ=255) gggGGAAGGCTACAAAAAAGGTATCGATAGCTGGGCTGCGGCGAACGATCGTGTAGCCAAAGCGATGATGGATAAACGCCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGGGTGGTTCTGCGGCACAGATTCGGCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCAtctc < 1:83116/300‑1 (MQ=255) cGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGAGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTAcctgcctcttataaaca > 1:620915/1‑285 (MQ=255) cTACAACAAAGTTATCGATATCTGGGCTGCGGCTAACGATCGTGTATACAAAGCGATGATGGATAAACTGCCAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTACGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGGCTGATGGCGAAGCCGGCTGGCTCCATCATCGAGACGCCACTCCCCTCGAACTTCCGTGCAGGTCTGCACGTACTCCA‑GTACGTACTCTCCACCCCCggg > 1:156979/1‑300 (MQ=255) cTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGACACCGTGATTAACAGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGt > 1:81794/1‑301 (MQ=255) acaacaAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCgg > 2:222110/1‑151 (MQ=255) acaacaAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCACTCACCGCGAACTTCCTTGAAGGTCTGAACGTACTCCA‑GTACTTc > 1:229763/1‑284 (MQ=255) acaacaAAGTTATCGATATCGGGGCTGCGGCGAACGATCGTGGATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCgg < 1:222117/151‑1 (MQ=255) caacaaAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGCTTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTAGCCGTGGTCTGATGGCGACGCCGGATGGCTCCATCATCGACACGCCAATCACCGCCAACTTCCGTGAGGGTCTGACCGTCACCCA‑ATCCTTCCGCCCCACCCCCGGtcc > 2:173623/1‑298 (MQ=255) caacaaAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCACCAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCTTCTCCACCCACGGTGc > 1:108132/1‑300 (MQ=255) aTCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGCTGGCGAAGCCGGATGGCTCCACCATCGACACGCGCATCCCCGCGAACTTCCGCGAAGGTCTGACCGTACTCCA‑GTACTTCCTCCCACCCCACGGTGCTCGTACAGGt > 1:171028/1‑300 (MQ=255) aTCGAGATCTGGGCGGCGGCGAAAGATCGGGTAGCCAAAGCGATGATTGATAACAGGCAAACTGAAACCGTGATTAACCGTGACGGGCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTc < 2:37895/301‑1 (MQ=255) atatCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGCACTTCCGTGAAGGTCTGCACGTACTCCA‑GTACTTCCTCTCCACCCCCGGTCCCCGTAAAGGTCTgg > 2:186309/1‑300 (MQ=255) ggCTGCGGCGAACGATCGTGTATCAAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAc < 1:331538/300‑1 (MQ=255) gcgAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCCGTTACCGccatga > 1:389479/1‑296 (MQ=255) aTCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCCCGGTGCTCGTAAAGGGCTGGCGGATACCGCACTGACAACTg > 1:606259/1‑300 (MQ=255) aTCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGAGAAGCCTGATGGCTCCATCATCCAAACGCCAATCACCGCTCACTTCCGTGAAGCTCTGACCGTACTCCA‑GTACTCCATCTCCACCCACGGTCATCGTAAAAGCCTGCCGGAGAcccacctgaacccgc > 1:55283/1‑287 (MQ=255) cGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCCTCGAAACGCCAATCACCGCGAACTTCCGTGACGGTCTGAACTTACTCCA‑GTACTTCCTCTCCACCCACGGCCCTCGCAAAGGCCAGGCGGATACCGCACTGAAACCtccca > 2:129814/1‑297 (MQ=255) cGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAACCGCCACTCACCACGACCTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCACCGGTGCTCGTAAAGGTCTGGCGGATACCGAACTGAACACTGCGa > 2:626547/1‑301 (MQ=255) aTCCAAAGCGATGCTGGATAACCTGCAAACTGCAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTTCGCGTGGTTCTGCGGCACAGCTTAGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCACTCACCGCGAACTTCCGTGAAGGTCTGCACGTACTCCC‑GTCCTTCATCTCCCCCCCCGGTACTCGTAAAGGTCTGGCGGCTCCCGCACTGACAACACCGAACTc > 1:13116/1‑300 (MQ=255) aTCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCGACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCAATCATCGAACCGCCCAGCCCCGCGCACTTCCGTGGAGGTCTGAAACTACTCCC‑GTCCTTACTCCCCACACACGGTGCCCGGAAAGGTCTTGCggcacccgccctcacccctcgcagctc > 2:712245/1‑275 (MQ=255) cgagtatgGATAACCTGCAAACGTAAACCGGGATTAACCGGGAAGGTCAGGAATAGAAGCAGGTTTCCTTCAACAACATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAcc < 1:689169/295‑1 (MQ=255) cGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAcc < 1:262901/300‑1 (MQ=255) atgatgGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGa > 1:228402/1‑301 (MQ=255) gatgGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTg < 1:94232/98‑1 (MQ=255) gatgGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTAAACAGCATCTACATGATGGCCGACTCCGGTg > 2:94228/1‑98 (MQ=255) atgGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACGCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCAAATCACCGCGAACTTCCGTGAAGGTCTGACCGT‑CGCCACGTACTTCATCTCCAACACCGGTCCTCGTAAAGTCCTGGCGCCTCCCCCACGTAACACTGCCACCTCCGTTCCCCTGaaca > 2:480153/1‑298 (MQ=255) tgGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGTCACAGATTCCTCAGCTTGCTGGTATGCTTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGCCATTCCGTGCAGGTCTGACCGTACTCCA‑GTACTTCATCTCCACCCCAGGCGCCCGTAAAGGTCTTGAGGCTGCCGCACTGAACAcgacacactcaggttcccgtacttg > 2:524571/1‑277 (MQ=255) ccTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGACGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGCAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCCCGTAAAGGTCTGGAGGTTACCGCACTGAAAACTTCGAACTCCGGTTACCTGAGTcgtcgt > 2:203564/1‑298 (MQ=255) aCCGTGATTAACTGTGACGGTCAGGAAGAGAGGCATGTTTCCTTCAACAGCATCTAGGTGATGGCCGACTCCGGTTCGCGTGGTTCTGCGGCACAGATTCGGCAGCTTGCTTGTAGTCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcgc < 1:626572/301‑1 (MQ=255) gTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCAACGCGAACTTCCGTGAAGGTCTGAACGTGCTCCA‑GTACTTCATCTCAACCCACGGTGCTGGTCAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACCCCGGTGACTTGAACCGTCGTCGGGTGGACGGGGCGCatg > 1:372256/1‑299 (MQ=255) gATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGCCGTGGCGCCGGa > 1:175077/1‑300 (MQ=255) aaatAACCGGTACGGTCAGACAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGGGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATTGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGAc < 1:518587/298‑1 (MQ=255) aCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGACAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGCCTTGGCGCAGCACCtggt > 1:239183/1‑301 (MQ=255) ccGTGACGGTCAGGAACAGAAGCAGGTTGCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCTCGTGGTTCTGCGGCACAGAGTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtggtg < 1:129818/301‑1 (MQ=255) cGTGACGGGAAGGAAGGGAAGCAGGGTTCCTTCAACAGCAGCTACATGATGGCCGGCTCCGGTGCGCGTGGGTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtggtgg < 2:228396/301‑1 (MQ=255) tCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGt > 2:84678/1‑195 (MQ=255) tCAACAGCCCCTACGTCATGGCGGACTCCGGGGCGCGTGGTTCCGCTGCACCGATTCGCCAGCTTGCTGGTAGGCGTGGGCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCACTCACCGCGAACTTCCGTGAAGGTCGGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGAGGATACCGCACTGAAAACTGCGAACTCCGGTTAACTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAgg < 2:86448/301‑1 (MQ=255) tCAAAAGCATCTACATGATGGCCGACTCCGGTGCGCGGGGTGCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCGGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAgg < 1:186316/301‑1 (MQ=255) | TGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTGGTCTGGTAACTGCGGGCGAACGCTACAACAAAGTTATCGATATCTGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCA‑GTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGG > NC_000913/4187209‑4187805 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |