Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,815,810 G→T intergenic (‑42/+24) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8100GT97.3% 223.9 / NA 77intergenic (‑42/+24)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (35/38);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAG  >  NC_000913/3815515‑3816102
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                    
ggTGCCACAGCGGGTGTGGGTGGCATCGTGATCCCTTTGAAAACAGTGCCAAACAGCAGCTCGAACTCCATGCGGGAATCCACCACGGCTTCAGCGTAAAAACGGCCTAACGGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAGTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAACCTTTAACACCTGCTTGCTAAGCGCAAATTCGATAAACTGGCGTTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGCCt                                                                                                                                                                                                                                                                                                 <  1:455463/300‑1 (MQ=255)
                  ggtggCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATCGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCGGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCAAAAAAAAGGCGAACCATCATTCGCCTTaaa                                                                                                                                                                                                                                                                                 >  1:465454/1‑300 (MQ=255)
                  ggtggCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTACCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACCGACAAAAAAAACTCTACTCATCAGTCGCCTTaaa                                                                                                                                                                                                                                                                                 >  2:649460/1‑300 (MQ=255)
                     ggCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCCTCTTACTTTTCTCCAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATc                                                                                                                                                                                                                                                                             >  2:122371/1‑301 (MQ=255)
                       cAACCGGGATCCCTTTGTAAGCAGGGCCCAACCGCAGATCCACCTCAATGCCGGAATCCACCACCGCTTCAGCTTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAg                                                                                                                                                                                                                                                                           <  1:122338/301‑1 (MQ=255)
                       cAAACGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAg                                                                                                                                                                                                                                                                           <  2:459272/301‑1 (MQ=255)
                        aaTCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGTTAAGCGCAAATTTAACAAACTGGCGCAGATATGGTTTAATGCCTTCGCTACTCATCTTACTTTTCTACCGACATAAAAACTACGACTTATCAGTCGACATaaaatacaat                                                                                                                                                                                                                                                                          >  1:325366/1‑295 (MQ=255)
                        aaTCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTACCAGATCGCTCCCGGTCTTAAACAACCCGGCGTTAAAGAAACATGGGCTTTTGCGCCCGGATTTCAACGTCAACTCGCCAACCTTTAACACCTGCTTGCTAAGCGCAAATTCAATCAACTTGCGCTGATATGGTTTCAGACCTTCCCTACTCATCTTACTTCTCTCCCTACAACAAAAATTCCACTCATCAGttccctacaaacacatt                                                                                                                                                                                                                                                                          >  2:311245/1‑285 (MQ=255)
                            gggATCCCTTTGTAAGCACGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                                                                                                                                                                                                                                                      <  2:675499/301‑1 (MQ=255)
                              gATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCCCATCTTACTTTTCTACAGACAAAACAAAGACGCCTCATCAGTCTCCTTAAAAATCAGTTTcccc                                                                                                                                                                                                                                                                    >  1:252511/1‑300 (MQ=255)
                                    tttGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATTCCTTCGCTCCCCATCTTACTTTTCTACATACAAAAAAAAGTCGACTCATCAGTCTCCTTCAAAATACGTTTGCCatcaccc                                                                                                                                                                                                                                                              >  2:555178/1‑295 (MQ=255)
                                    tttGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTTCCAGcgccc                                                                                                                                                                                                                                                              >  2:391804/1‑300 (MQ=255)
                                          agcaccgcccaaccgccaaTCGAACTCAACGCCGGAATCCCCCAACGCTTCAGCGTAAACACGGCCGACCAGTGCCAGATCGCGACCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                                                                                                                                                                        <  1:522206/283‑1 (MQ=255)
                                          aGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTGACTTTTCTACAGACAAAAAAAATTCGACCCATCAGTCCCCGTAAAAATCAGCTTGCCaaccctccactct                                                                                                                                                                                                                                                        >  2:651491/1‑289 (MQ=255)
                                                   aaaCAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACtcatc                                                                                                                                                                                                                                                                                             <  2:88707/255‑1 (MQ=255)
                                                   aaaCAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAATCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCt                                                                                                                                                                                                                                                >  1:150517/1‑300 (MQ=255)
                                                           gATCGAACTCAATGCCAGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGACAAATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGAATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTATCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                                                                                                                                                                                                                                       <  2:325468/301‑1 (MQ=255)
                                                             cccaaCACAATGCCTGAACCACCAGACCATTCATCGTAAAACCTGCCTAACAGGGCCAGATCGCTCCCGTTATTAAACCGCCCGGCGTTGCAGAAATAGGGGCTTTTGCTCCCGGATTTCAGCGTAAACTCGCAAAACTTTAACTCCGTCTTGCTAAGCTCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACCGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattc                                                                                                                                                                                                                                     <  1:197084/298‑1 (MQ=255)
                                                                   ccAATGCCGGAATCAACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCACTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTcc                                                                                                                                                                                                                               <  2:413111/300‑1 (MQ=255)
                                                                         ccaatctCCACCCACGCTGCAGCGTAAAAACCGCCTAACAGTGGCAGAGTGCGCACGGTATTAAACAGCCCGGTGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGGCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg                                                                                                                                                                                                                         <  1:583042/295‑1 (MQ=255)
                                                                         ccGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGCCGCCTTAAAAATCAGTTTGCCAGAGCCGCCTTCCGGCTCCCTCCAATGGATCCGATTCCCCTCgg                                                                                                                                                                                                                         >  1:289757/1‑301 (MQ=255)
                                                                                ccaccaACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCCCCTCATCTTACTTTTCTACAGACAAAAAAAAATCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCCCCGCCTTCTCCGTCTGTACCATGGATTCGATTCCCCTCggttatcgg                                                                                                                                                                                                                  >  2:583203/1‑294 (MQ=255)
                                                                                  accaACGCATAAGCGTAAAACCGGCAGTACACGGGCAGATCCCGCCCGGTATTAAACGACCCGGCGTTGAAGAAATAGAGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACGGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGcc                                                                                                                                                                                                                <  1:341584/301‑1 (MQ=255)
                                                                                   cacacccctgcAGCGAAAAAACGGCCTAACAGGGCCGGATCGCGCACGGTATTAAACAGCCCGGCGTTGAAGAAATAGGCGCTTTTTCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATGCAATAAACTGGAGCTGATATGGTTTCATGCCTTCGCGCCTCATCTTACTTTTCGACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGcc                                                                                                                                                                                                                <  1:297835/291‑1 (MQ=255)
                                                                                    ccgcaccttcgcccaaaaacCGGACCAACCGTACCCAATCTCGCCCGGTATGAAACAGCCCTGCCTATAAGAAATGGGTGCTTTTCCGCCCAGATTGCAGCGTAAACTCGCCAAACTTTAACACCTTCTTGCTAAGGGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCCCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAGTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                                                                                                                                                                               <  1:651308/286‑1 (MQ=255)
                                                                                     aaacgtctcggcctAACAACGGCCTAACCGTGCTCGATCGCGCCCGGTATTAAACAGCCCGGCGGTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGGAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTAAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTGTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaa                                                                                                                                                                                                              <  1:649277/288‑1 (MQ=255)
                                                                                     aaCGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCCGGCCCAGCGCccac                                                                                                                                                                                                             >  2:151006/1‑301 (MQ=255)
                                                                                       ccGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaaca                                                                                                                                                                                                            <  2:289839/300‑1 (MQ=255)
                                                                                         ccTTCAGCGTAAAAACGACCCACACGTGCCAGACCGCGCCGGGTATTAAACAGCCCGGCGTGGAAGAAATAGGGGCTTTTGCGCCCGGATTACAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGAGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAg                                                                                                                                                                                                          <  1:49906/300‑1 (MQ=255)
                                                                                           ttCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACATGCTTGCTAAGCGCAAATTCAATAAACTGGCGCCGATATGGTTTCATGCCTTCGCCCTTCACATTACTTTTCTACAGACAAAAAAAAGTACACACATCAGTCGCCGTAAAAAACAGTTTGCAAGCCACGCCTTTGGAGTCGTTACCCTGGATTCTCATCCCCCCGGGACCGAGCCAAAAAGa                                                                                                                                                                                                         >  1:187509/1‑300 (MQ=255)
                                                                                            tCAGCGTAAAAACGGCCTAACAGGTCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGa                                                                                                                                                                                                         <  2:628768/299‑1 (MQ=255)
                                                                                               ccagccaaaCGGCACAACAAGGCAAGACCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                                                                                                                                                                    <  1:393773/295‑1 (MQ=255)
                                                                                                     aaaCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCCGAGCCAACAAGCTGaat                                                                                                                                                                                                    >  2:393891/1‑293 (MQ=255)
                                                                                                          gCCCAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                                                                                                         <  2:150559/301‑1 (MQ=255)
                                                                                                            cgaaCAGTTCCAGATCTCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGAAAATTCAATAAACTGTCGCTGATATGGTTTCATGCCGTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCATTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATggg                                                                                                                                                                                       <  2:187561/299‑1 (MQ=255)
                                                                                                                       aGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCTCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                                                                                                            <  2:252579/301‑1 (MQ=255)
                                                                                                                               cccGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc                                                                                                                                                                    >  2:563142/1‑301 (MQ=255)
                                                                                                                                ccGGTATTAAACAGCCCGGCGTTTAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTTATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                                                                                                                   <  1:562987/301‑1 (MQ=255)
                                                                                                                                         aaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc                                                                                                                                                          <  2:465585/301‑1 (MQ=255)
                                                                                                                                               ccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCATCGCCGCCTTCTGCGTCGCTACAATGGATTCCATTCCCCTCGGGCCAGAGCCAACAAGATGAGTACCTCTTTCTGGGTGAACGGCTCGCCCTCTGCCGTCCccttcccttct                                                                                                                                                    >  1:303607/1‑300 (MQ=255)
                                                                                                                                               ccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc                                                                                                                                                                                                                       >  1:391006/1‑234 (MQ=255)
                                                                                                                                               ccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc                                                                                                                                                                                                                       <  2:391124/234‑1 (MQ=255)
                                                                                                                                                 ggCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCACGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGCTGAGTATCTATTCCTGGGTGAACGGCTCGCCTTCTGCCGTCGTCTGCCCTTCCat                                                                                                                                                  >  1:97826/1‑301 (MQ=255)
                                                                                                                                                    gTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCCCTTCAatgat                                                                                                                                               >  2:370745/1‑301 (MQ=255)
                                                                                                                                                       gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACact                                                                                                                                                                                                                                           >  1:214095/1‑204 (MQ=255)
                                                                                                                                                       gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt                                                                                                                                                                                                                                           <  2:214152/206‑1 (MQ=255)
                                                                                                                                                                     ttttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTTCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                                                                                                                                                                          >  2:443900/1‑241 (MQ=255)
                                                                                                                                                                     ttttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                                                                                                                                                                          <  1:443776/241‑1 (MQ=255)
                                                                                                                                                                         gcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATTATGCGCCCGTCTTCGGTCATCAc                                                                                                                          >  2:132124/1‑301 (MQ=255)
                                                                                                                                                                           gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                                                                                                                                                                          >  1:237628/1‑235 (MQ=255)
                                                                                                                                                                                gATTTCAGCATAAACTCGCCAAACTTTAACTCCTGCTTGCTAAGCGCAAATGCAAGAAACTGGCGCTGATATGGTCTCATGCCTTCGAGCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                                                                                                                   <  1:150964/301‑1 (MQ=255)
                                                                                                                                                                                     cAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTTCCAGCACCGCCTTCTGCGTCGCTACCATGGATTCGATTCCCCTTGGGCCAGAGCCACCAAGTTGAGTAGCTCTTCATGGGCAACCGGCTCGCCTTCTGCCGTCCACTGCCCTTCAATGCTGCACCCGTCTTCGGCCATCACTACGTTCATTTc                                                                                                              >  1:76885/1‑301 (MQ=255)
                                                                                                                                                                                         gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAAGCCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCCCCCGCCTTTGGTCATCAATACGTTACTGTGGGtc                                                                                                          >  2:662185/1‑301 (MQ=255)
                                                                                                                                                                                            aaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc                                                                                                                                                          <  2:263945/250‑1 (MQ=255)
                                                                                                                                                                                               ccGCCAAAATTAAAAACCTTCTTGCTAAGCGCAAATTCAATAAGCTTGCGCTGATATGATTTCATTCCTTCGCTTCGCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCATTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATGCGACTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCg                                                                                                    <  2:76904/300‑1 (MQ=255)
                                                                                                                                                                                                cctcttcccctctcacCTTCTTGCTAAGCGCCAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCgg                                                                                                   <  2:627669/288‑1 (MQ=255)
                                                                                                                                                                                                            acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTATTCATGGGTGAACGGCTCGCCTTCTGCCGTCCACTTCACTTCAATGATGCGCCCGGTTTCGGTCCTCACTCCGTTACTGTCGGTCCTTGTGGCAGAGCATTCa                                                                                        >  2:401037/1‑300 (MQ=255)
                                                                                                                                                                                                             cacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCCGGGCCAGAGCCAACAAGATGAGTAGCTCTCCATGGGTGAACGGCTCGCCTTCTGCCGCCCCCTCCACTTCAATGATGCGCCCGTCTTCGGTCGTCACTACGCTCTTGTCGTTCCCTGCggagttcggcagagc                                                                                      >  1:627492/1‑288 (MQ=255)
                                                                                                                                                                                                                          ggcgagaattcactgattttgcgcGGATATGTTTTCCTACCTCCGCTCCGCATCTTACTTTACTACAAACAAAATAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAGTGGTTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCg                                                                         <  1:60165/281‑1 (MQ=255)
                                                                                                                                                                                                                                     aaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCGTCACTAAGTTCATGTCGGTCTCTCCGGCAGAGTCTTCAACGTCTTCCAGATTGCAAACCGCTTc                                                              >  2:341568/1‑301 (MQ=255)
                                                                                                                                                                                                                                               cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACCAGATGAGTAGCTCTTCATGGGTTAACGGCTCGCCTTCTTCCGTCCCCTGCCCTTAAAGTATCCGCCCGTATTCGGTCATCACTACGTGTATGCCTGTCACTGCGTCCGGGTCTTCCACTTGTTGCTCGTCGCAAACCGCCTCGCCGTTcacc                                                    >  1:199753/1‑300 (MQ=255)
                                                                                                                                                                                                                                               cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTCCGTTCATGTCTGTCTCTGCGGCAGAGTCTTCACCGTATTCCAGATCGCAAACCGCTTCGCCGTTcccc                                                    >  2:309698/1‑298 (MQ=255)
                                                                                                                                                                                                                                                             cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCCTCACTACGTTCATGTCGGTCTCTGCGGCCGAGTCTTCAACGTATTCCAGATCGCACACCGCTTCGCCGTTTAAAATTCCGACAGCAAc                                      >  2:299957/1‑301 (MQ=255)
                                                                                                                                                                                                                                                               tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                                                                                                                 >  1:64702/1‑208 (MQ=255)
                                                                                                                                                                                                                                                               ggCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTTAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                                                                                                                 <  2:64716/207‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ttcgcccctccgtctatattcacaCAGACAAAAAAAGCGCCACCCATCAGTCGCCTTACACATCAGTTGGCGAGGGACGCTTTCTGAGTCGCTACAATGAATTCGATTCGCATCGGGCCAGAGCCAAAAAGATGAGTAGCTCTTCTTGGGTGAACTGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCgg                                 <  1:662000/277‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct                                                                                                         >  1:84980/1‑219 (MQ=255)
                                                                                                                                                                                                                                                                            cATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct                                                                                                         <  2:85000/219‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCCGATCGCAAACCGCTTCCCCGTTCCCAATTCCGACAGAAACAGCGGCTACCATCCCttt                    >  1:64519/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                 tcagtttCAACAGACAAAAAAAAGTCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGCGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCtt                     <  2:539215/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        cgaccgACCAAAACAATTCGACTCCGAAGTCGCCTTAAAAATCTGTTACCCAGCTCCGCCTACTGCGTCGCTACCATGGATTCGATTCCCCACGGGCCATAGCCAACACGTTGATTAGCTCTTCATGCGTGAACGGCTCGCCTTCTGCCGTCCCCTTCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGAtt           <  1:311144/296‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          aCAGCCAACAAAAAGTCCCCTCCGCAATCGCCTTAACAATCAGTTTGCCAGCGCCGCCTTCTGCCTCGCTACAATGGATTCGATTCCCCTCGGGCCAGATCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTg          <  1:370629/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          aCAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTgg         <  2:64533/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                              aCAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAAGGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATGGGtttt     >  2:682132/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                               cAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCCCCCGTCTTCGGTCATCACTACCTTCATGTCCGCCTCTGCCGCCGAGTCTTCAACGTATTCCCGTACGCAAACCGCTTCCCCGCTCACCATTCCGACAGCAACGGGGCCTACACACACATTCATCGGATTGGgttcc    >  1:498802/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                aaaaaaaaGTTGACCCCGCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGGGTCGGTAAAATGGAGTCGATTCCCCTCGGTCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCa   <  1:132087/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                aaaaaaaaGTCGACTCATCAGTCGCCTTAGAAATCAGTTTGCCAGCGCCGCCTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTATCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCATATCGCAAACCGCTCCCCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCCGATTGGtttgcgg  >  1:157961/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                    
AGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAG  >  NC_000913/3815515‑3816102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: