Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,296,191	0	A	C	36.6%	13.1 / 34.2	41	intergenic (+397/+245)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base A (15/11);  new base C (13/2);  total (28/13)
Fisher's exact test for biased strand distribution p-value = 8.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCC  >  NC_000913/4295892‑4296483                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                         gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTATGCCGCATCCGTCATCCACTCCTGATGCGACGCTTAAc                                                                                                                                                                                                                                                                                                         >  2:849569/1‑301 (MQ=255)                     ggcgtatagggcctatatgcctcacacaccATCCACGCCTGCTGTGACCC‑TTTCCGCTGCTTATCAGCCCTACTCCAAAAAGCGAAATAGCCTGTTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACACCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGc                                                                                                                                                                                                                                                                                     <  1:672116/272‑1 (MQ=11)                                        ccGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCCACGCCTGATGCGACGCTTAACGCGTCTTATAAGGCCTACGCCAGACCGCGCAATAGTCTg                                                                                                                                                                                                                                                                  >  2:612834/1‑301 (MQ=21)                                        cagcagacaacgtCAACGCATGATGC‑ACACGTTAACGCGGGTTATAAGGCCTAAGCGAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACAGCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                                                                                                                                                                                                                                                  <  2:287730/289‑1 (MQ=11)                                                           gttctgcatcGC‑TTACCGCGTCTGATGCGGCCTACGCCAGCCAGAGCAATGGTATGATTGTGGGTGAATTGTGAGGTCGGGTAAGGCGTTTTCCCCGCATCCGAGATCAACGCCGGATGCGACGCTCTCCGCGTATTACCAGGCCTACGCCAGACAGCGCAATAGCAGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                                                                                                                                                                                                                                               <  1:929965/292‑1 (MQ=11)                                                            tGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATTCGACGCTTAACGCGTCTTATCAGGCCTACTCCAGACAGCGCAATAGCCTAATTTAGCGTGATTTTGTAgg                                                                                                                                                                                                                                              >  2:747464/1‑301 (MQ=21)                                                               aaacgcacattctcccgTCCTTTCAGGCTTCGGCCAGACATGCAAATAGGCTGATTTGGCGTGATTTTGTAGTTCGGCTAAGGCGTTTACGCCGCATCCGCCATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAATGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                                                                                                                                                                                            <  1:161661/286‑1 (MQ=11)                                                                 gcgaacgttACCGCTTCTTGTAAGGCTTACGCTAGATGTTTCATTAGCCTGATTTGGCGTTATTTTGTAGTTCGTATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAAAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                                                                                                                                                                                                                                           <  1:615272/293‑1 (MQ=11)                                                                        ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAtgcggc                                                                                                                                                                                                                                   >  2:991027/1‑296 (MQ=21)                                                                            cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCCACGCTTACCGCGTCTTATCAGGCCTACGCCAGACCGCCACATAACCTCCTTCCCCGTGATTTTGTAGGTTGGATAAggaggtt                                                                                                                                                                                                                               >  1:1123504/1‑296 (MQ=11)                                                                                    aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                                                                                                                                                                                            >  2:161695/1‑280 (MQ=33)                                                                                             cctccAGACAGCGCCATGTCCTGTTTTAGCGTGATTTGGTAGGTAGGATAAGGCGTTTACGCCGCCTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                                                                                                <  2:787803/296‑2 (MQ=11)                                                                                              cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACCCCGCATCCGActgc                                                                                                                                                                                                              >  2:122550/1‑297 (MQ=21)                                                                                               gcgagatggcgaaaatgCCTGATTTAGGTTGATTTTGTAGGTCGGAGAAGGCGTTTACGCCGCATCCGACATCAGCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaa                                                                                                                                                                                                            <  2:366185/285‑1 (MQ=255)                                                                                                ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTACGCCCACCGTTGCAATATCCTGATCTCGCGTGATTCTGTCGTTCGGATAAGGCGttttgcgctcttccaccctctat                                                                                                                                                                                                           >  1:241099/1‑281 (MQ=11)                                                                                                     cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTATGCCGCATCCGACATCACCCCCTGATGCGCCGCTTACCGCGTCTACTCAGGCCTACCCCACACCGCACAAAATCCTGCTGTATCGTGACTTTCTAGGTCGGGTGCGGCGTTTACGCCTCATccataacaaatgcctt                                                                                                                                                                                                      >  1:543900/1‑287 (MQ=11)                                                                                                              gatatcatatggCGTGATTTTGTGGGTCGAATGAGTCGTGTACCCCGCATCCGACTACCAGTCCTAATGCTACGCTTGCCCCGTCTTATCAGGCCAACCCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTATGCCGCATCCTACATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                                                                                                                             <  2:1005934/290‑1 (MQ=11)                                                                                                              aGCCTGATTTGGCGTGAGTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCCACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg                                                                                                                                                                                              <  2:93831/300‑1 (MQ=255)                                                                                                                  tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGGTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGACTGATGGAACGCtttc                                                                                                                                                                                         >  1:15766/1‑299 (MQ=255)                                                                                                                           gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCttt                                                                                                                                                                                >  2:307684/1‑300 (MQ=12)                                                                                                                               ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTTCGCCGCATCCTACATCAATGCCTGAGCCGACGCTTGCCGAGTCTTATCat                                                                                                                                                                            >  2:640917/1‑300 (MQ=255)                                                                                                                                    tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                                                                                                                                                                                            <  1:1160703/232‑1 (MQ=18)                                                                                                                                    tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                                                                                                                                                                                            >  2:1160993/1‑232 (MQ=33)                                                                                                                                                 gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg                                                                                                                                                                                                                                     >  2:1057145/1‑226 (MQ=33)                                                                                                                                                           cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtaaa                                                                                                                                                                                                                              >  2:892619/1‑220 (MQ=17)                                                                                                                                                           cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGATTAACGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACCTCAATGCCTGATTGGACGCTTCCCGCCTCCTATTAGGcacaacttaacctattgttcatttttt                                                                                                                                                >  1:796615/1‑275 (MQ=11)                                                                                                                                                                  gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                                                                                                                               <  2:139575/247‑1 (MQ=17)                                                                                                                                                                  gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                                                                                                                               >  1:139547/1‑247 (MQ=17)                                                                                                                                                                                                                                                                aGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGTCCTATCTTAACCGtt                                         >  2:587037/1‑301 (MQ=9)                                                                                                                                                                                                                                                                   ggTTTACGCCGAATCTGACAGCAGCGCGTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGGGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt                                      <  2:66890/300‑1 (MQ=11)                                                                                                                                                                                                                                                                              cATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACCGTTGGTTAATTTTTCa                            >  2:264603/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACCTCAATGCCTGATGCGACGCTTGCCGGCGCTTAACAGGCCTATCTTAACCGTTTGTTATTTTTTCaaa                          >  1:852581/1‑300 (MQ=11)                                                                                                                                                                                                                                                                                     cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTCCG‑‑CCGCAGCCGACATCAATGCCTGATGCGCCGCTGGCCGCGTCTTACCAGGCCTATCTTAACCGTGCGTTCATTTTTGAAAcctttc                    >  1:260104/1‑296 (MQ=11)                                                                                                                                                                                                                                                                                        gtaggCCTGATAAGACGCGGCAAGCGTCGCATCAGGCGTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTTGCGTGATTTTGTAGGTCGGATCAGGAGTTT‑‑ACGCCGCATCCGAAATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGACTGTATTAACCGTTGGTTAATTTTGTAAAatctcaacc                 >  2:313214/5‑296 (MQ=11)                                                                                                                                                                                                                                                                                        cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcac                  >  2:821941/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGGGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACCTACATGCCTGATGCGACGTTTTCCCCGTCTTAACGGGCTTTATTTAACCGTTGGTTAATTTTTCAAACCCtttccc                 >  1:84723/1‑297 (MQ=11)                                                                                                                                                                                                                                                                                         aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCTCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTAacac                >  2:630668/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                               tGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGCCGCTGGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGtt          >  2:651056/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                gATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGCCACCAATGCCCGATGCGACGCTTGCCGCGTCTTATCAGGCCCATCTTAACCGTTGGTTAATTTTTCAACCTTTTCACACCCCGttt         >  1:1160924/1‑299 (MQ=11)                                                                                                                                                                                                                                                                                                 aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATc        >  2:182394/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                       cGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc  <  2:1123789/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                         GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCC  >  NC_000913/4295892‑4296483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑