Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C93.4% 259.4 / 12.1 93intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (2/4);  new base C (47/38);  total (51/42)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
*NC_0009134,296,3802.G93.5% 259.5 / 11.3 93intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (2/4);  new base G (49/37);  total (51/42)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGT  >  NC_000913/4296144‑4296671
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                                                                      
tAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTAACCg                                                                                                                                                                                                                                          >  2:445001/1‑301 (MQ=2)
  aGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTTCCTGGCCTCTCTTAACCGtt                                                                                                                                                                                                                                        >  1:827344/1‑301 (MQ=11)
     cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCGGATGCGCCGCTTGCCGCCTCTTATCAGGCCTATCTTAACCGTTGGt                                                                                                                                                                                                                                     >  1:723603/1‑301 (MQ=255)
           cgCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGGTGCGACGCTTAACGCGTCTTATCAGGCATACGCCAGACAGCGCAATAGTCTGATTTAGCGTGATTTTGTAGTCCGGCAAAGGC‑GTTT‑‑AAGCCCCTTCCGACATCGAATCCCGATCAGACGCTTCCCTCGCTTTCTCCAGTCTATCTTCATCGATGGTTAActt                                                                                                                                                                                                                               >  1:829391/2‑301 (MQ=11)
           cgCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCGTATCCGGCCTATCTTAACCGTTGGTTAAttt                                                                                                                                                                                                                               >  2:848669/2‑301 (MQ=255)
            gcccaatacGACATCAACTCTCGATGCGACGCTTGCCTCCTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtttt                                                                                                                                                                                                                              <  2:710959/293‑1 (MQ=11)
            gCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtttt                                                                                                                                                                                                                              >  2:1155189/1‑301 (MQ=255)
             ccgctcccGACAGCACTGCCGGGTGCGCCGCTTGCCTCGTCTTATCAGGTCTACGCCGGACTGCGCAATAGGCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                                                                                                                                                                                             <  1:846643/295‑1 (MQ=11)
                    cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTCCG‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTATCTTACCCGTTGGTGAATTTTTCaacatc                                                                                                                                                                                                                      >  1:489143/1‑297 (MQ=11)
                        aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCTACGCTTGCCGCGTCTTATCCGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                                                                                                                                                                                                   >  1:766848/1‑300 (MQ=255)
                              gCCTGATAAGACGCGGCAAGCGTCGCATCAGGCCTACGCCAGGCAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCCCCg                                                                                                                                                                                                            >  2:648114/1‑301 (MQ=14)
                              ccacactgctactcttgccgagccTTAACCGGCCTACGACCAACACCTCAATAGCCTTATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGTGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCg                                                                                                                                                                                                            <  1:226648/278‑1 (MQ=14)
                               ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTCCG‑‑CCGCATCCGCCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTCATTTTTCAAACTCTTCTCCCCCgg                                                                                                                                                                                                           >  2:44852/1‑301 (MQ=255)
                                cggATCCGACGCTTGCCCCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt                                                                                                                                                                                                          <  2:1029775/299‑1 (MQ=255)
                                 tGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATTCCTGATGCCACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGtt                                                                                                                                                                                                         >  2:216775/1‑300 (MQ=255)
                                  gtggCGTCGCTTCCCGAGTCTTATCAGGCCTACGTCAAACAGCGAAATTGCCTGATTTTGCGTGATTTTG‑TAGGTCGGATAAGGCGTTCATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTAt                                                                                                                                                                                                        <  1:1176435/298‑1 (MQ=14)
                                    tgagccgctggccgctttttgtacggCCTACGACAGACAGCGCAATAGTCTGCTTTAGGTTGATTTTG‑TAGGTCGGATAAGGGGTTGATTCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATc                                                                                                                                                                                                       <  1:1165077/276‑1 (MQ=14)
                                      cGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCGTATCATGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATcca                                                                                                                                                                                                     >  2:1078664/1‑298 (MQ=255)
                                       cacgctggccgcgTCTTATCAGCCCTAAGCCAGACAGCGCAATCGCCGGATTTAGCGTGATTTTT‑TAGGTCGGCTAAGGCGTTTATGCCGAATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                                                                                                                                                                   <  1:1164457/292‑1 (MQ=14)
                                        gccatgcaCGTGTCTTATCGGGCCTACGCCAAACCGCGACATAGCTTGATTTAGCGTATTTTTG‑TAGGTCGTATAAGGCGTTTAGGCCGCTTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc                                                                                                                                                                                                  <  1:1154882/294‑1 (MQ=14)
                                         cGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACACGGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGGTAATGC‑GTTT‑‑ACGCCGCACCCGACATCCATCCCGAAGGCGACGCCTGCCGCGTCTTACCAGGCCTATATTAACCGTTGGTTATTTTTCTCAACTCTTCCATCCCGGTACAAAAccc                                                                                                                                                                                                 >  1:379556/1‑301 (MQ=11)
                                             tgccgctccgtATCACGCCTACGCCATACAGCTCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGTATAAGGCGTTTATTCCCCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc                                                                                                                                                                                              <  2:379671/290‑1 (MQ=14)
                                              gccgcgTCTTATCATGCTTACGACAGAAAGCCCAATAGCCTGATTTAGCGT‑ATTTTGTTAGTTCGGAGAAGCCGTTAATGCCGCATCCGAAATAAACGCCTGATGCGACGCTTAAAGCGTCTTATCAGGCCTACGCCAGACAACGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGTCATCAATGCCTTATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCTAACTCTTCACACCCGGTATCAAACCCTTcc                                                                                                                                                                                             <  2:163366/298‑1 (MQ=14)
                                                 gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                         <  2:767073/301‑1 (MQ=255)
                                                 gagtctgttCAGGCCTACGCGATACAGTGCAAGAGCCTGATTTGTCTTGATTTTG‑GAGGTCGGCTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAAGGTTAACGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                         <  1:695837/293‑1 (MQ=17)
                                                       tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCCTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAATTCTTCACTCCCGGTATCATCACCTTCCCTACAGCTcc                                                                                                                                                                                   >  2:365928/1‑300 (MQ=14)
                                                         tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCTTTGGTTATTTTTTCAAACTCTTCCCCCCCGGTATCAAACCCGTCTCTACAACtacgg                                                                                                                                                                                 >  1:336487/1‑297 (MQ=14)
                                                               gtccggcggcaaGCGGAAGAGCCTGGTTTTACGTGATTGTG‑GAGGTCGGATACGGCGTTTATTCCGCATCCCGCATCACCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCGGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAAc                                                                                                                                                                          <  1:731794/290‑1 (MQ=17)
                                                                 aCGCCAGACTGCGCAATAGCCTGATTTAGCGTTATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                         <  2:687506/285‑1 (MQ=255)
                                                                 aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGC‑GTTT‑‑ACGCCGCATCAGACATCCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACAGTTGGTTAATTTTTCAAACTCTTCACACACGGTATCAAACCCTtccatccaaataggaagagcgtc                                                                                                                                                                         >  1:687294/1‑286 (MQ=14)
                                                                   ggacaaCAGCGCATTTGTCTGATTTAGCGTGTTTTTG‑TAGGTGGGATAGGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGt                                                                                                                                                                      <  1:516293/296‑1 (MQ=17)
                                                                       gACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACCCTTCACACCCGGTATCAAACCCTTCAATACAGTCCAG‑GCTCacacattgc                                                                                                                                                                    >  1:406078/1‑291 (MQ=17)
                                                                         cAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCCTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAATTAGCGTGATTTTGTAGGTCGGATCAGGC‑GTTT‑‑AAGCCGCCTCCGACCTCAATGCCTGCTGCGACGCTTGCCGCGTCTTAGCAGTCCTATTTCAACCGTTGGTTAATTTTTCACATCCTTCACCTCCTGTAGACTACTCTTCCATAAATCTCAGTGTTCacgaatacaccg                                                                                                                                                                 >  1:302015/1‑290 (MQ=11)
                                                                           gcgcAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGTTGCGACGCTTGCCGCGTCTTATCTGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGcc                                                                                                                                                              >  2:1122951/1‑301 (MQ=255)
                                                                              cAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCAGCCAGAAAGCTCAG‑GTTCAACCAGTGCAGCGCCttt                                                                                                                                                           >  1:595054/1‑301 (MQ=255)
                                                                               aaTAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCtttt                                                                                                                                                          <  2:827581/301‑1 (MQ=255)
                                                                                     cggATTTAGCGTGAGTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGGCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGt                                                                                                                                                    <  2:829628/299‑1 (MQ=255)
                                                                                             gCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTCCCATACAGCTCAG‑GCTCAACCAGTGCAGCGCTTTTTGCTTGTTTTTCTCg                                                                                                                                            >  1:602068/1‑301 (MQ=255)
                                                                                             gCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGACTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTtctct                                                                                                                                            >  2:306955/1‑300 (MQ=255)
                                                                                              cGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCCCAG‑GTTCAACCCGTTCAGCCCCTTTTGCTTGTttttcttgt                                                                                                                                           >  1:1212133/1‑298 (MQ=255)
                                                                                               gTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCCG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGAt                                                                                                                                          >  2:898708/1‑301 (MQ=255)
                                                                                               gTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAACCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCTCCTTTTGCTTGTTTTTCTCGAt                                                                                                                                          >  2:842500/1‑301 (MQ=255)
                                                                                                           ggTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCCGGGCCGCGCCTTTTGCTTGTTCTTCTCGATAA‑AAcaagtcc                                                                                                                              >  1:1160092/1‑295 (MQ=255)
                                                                                                           ggTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AAc                                                                                                                                    <  2:1160399/295‑1 (MQ=255)
                                                                                                                   aaGGCGTTTATCCCGAATCACACATAACCGCCGTATGCGACGCGTAACGCTGCTGATCCGGCCTACGCCAGACTGCGCAATAGCCTGATTTAGTGTGATTTTGTAGGTCGTATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGttt                                                                                                                      <  2:707703/301‑1 (MQ=21)
                                                                                                                    aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCttttt                                                                                                                     >  1:1048189/1‑300 (MQ=255)
                                                                                                                             tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCCTGATAA‑AACCTTCTTCCTCCTTTTAGAAcctc                                                                                                             >  2:165020/1‑300 (MQ=21)
                                                                                                                             tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGACTTGTCCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAAcatct                                                                                                            >  1:129859/1‑300 (MQ=255)
                                                                                                                                   aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTACCACCCGGTATCAAACCCTTCCATAGAGCTCCG‑GTTTAACACGTGCAGCGAATTTTCGTTGTTTTTCTCGCTAACAACCTCCTGC‑CCGTTTAAGAAcacatatcccc                                                                                                      >  1:265274/1‑292 (MQ=17)
                                                                                                                                      cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGccc                                                                                                   <  2:1020578/301‑1 (MQ=255)
                                                                                                                                      cGACATCAACGCCTGAGGCGACGCTTAACGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGccc                                                                                                   <  2:406204/301‑1 (MQ=255)
                                                                                                                                       ttccgacttctccgATGCGGCGCTCAAAGGTTCTGATCAGGCCTACGCCAGACAACGCAAAAGCCTGATTTAGCTTGATTTTTGAGGTCGGATAAGGC‑GTTTCCG‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGccc                                                                                                   <  1:668963/287‑1 (MQ=25)
                                                                                                                                         cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGt                                                                                                >  2:384473/1‑301 (MQ=255)
                                                                                                                                         aaTCAACGACTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCATCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGt                                                                                                <  2:595236/300‑1 (MQ=255)
                                                                                                                                          aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGt                                                                                                >  2:214715/1‑300 (MQ=255)
                                                                                                                                           tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTTTTCTTCTCGTTAA‑AACCTTCTTCACCGTTTAAGACAACCATTCCCGCCCAGT‑Ac                                                                                             >  1:517287/1‑301 (MQ=255)
                                                                                                                                                gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGcccctatcggaa                                                                                          >  2:669166/1‑291 (MQ=255)
                                                                                                                                                gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGa                                                                                        <  2:336592/301‑1 (MQ=255)
                                                                                                                                                 gcggcgggacgggatgagtcgTGTTACCAGGCCTAAGCCGGGCGGGGGTATAGCCGGATTTAGGGTGGTTTAGTAGGCTGGACTACGC‑GTTT‑‑ACGCCGCATCCGACGTCATTGCCGGATGCGACGCTGGTCGCGGCTTTTCAGGCCTATCTTAACCGTTGGTTAATTTTTCACACTCTTCACACCCTGTAGCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCCTTCCCGCCCAGT‑ACTCgg                                                                                         <  1:14416/280‑1 (MQ=255)
                                                                                                                                                 ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGaa                                                                                       <  2:1048469/301‑1 (MQ=255)
                                                                                                                                                   tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAC‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAAt                                                                                      >  2:941003/1‑300 (MQ=255)
                                                                                                                                                        gacctattAAGGCGTCTTATCAGGCCTACGCCCGACATCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCATTTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCGTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCgg                                                                                <  1:898457/295‑1 (MQ=37)
                                                                                                                                                         aaCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGt                                                                               <  2:723822/300‑1 (MQ=255)
                                                                                                                                                                  cgcgTCTTACCCGGCCGTCGCCCGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTGT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCGTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAAt                                                                      <  1:444867/301‑1 (MQ=255)
                                                                                                                                                                      tgttATCAGGCCTACCCCAGACAGGTCAATAGCCTTTTATAGCGTGTTTTTGTAGGTCGGGTAATGC‑GTTT‑‑ACGCCGCCTCCGACATCAAGGCCTGATTCGACGCTTGCCGCTTCTTATCATGCCTATCTTAACCGTTGGGTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGc                                                                   <  1:940742/298‑1 (MQ=255)
                                                                                                                                                                         tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCCCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAAACTCTTTCCC‑CCCACTCACTCGGAATAACCGGTTCGGCAAATTGCag                                                                 >  1:1157793/1‑299 (MQ=25)
                                                                                                                                                                          aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAAcc                                                                                                                                                                         <  1:1052029/196‑1 (MQ=255)
                                                                                                                                                                          aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAAcc                                                                                                                                                                         >  2:1052309/1‑196 (MQ=255)
                                                                                                                                                                             aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCATAGCTGc                                                           >  2:640989/1‑301 (MQ=255)
                                                                                                                                                                             aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGc                                                           >  2:1190584/1‑301 (MQ=255)
                                                                                                                                                                              ggCCTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCg                                                          <  2:489293/301‑1 (MQ=255)
                                                                                                                                                                                ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGATCTGCGtt                                                        >  1:622740/1‑301 (MQ=255)
                                                                                                                                                                                 cTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc                                                                                                                                                                                              <  2:1170708/169‑1 (MQ=255)
                                                                                                                                                                                 cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc                                                                                                                                                                                              >  1:1170394/1‑169 (MQ=255)
                                                                                                                                                                                    cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCTATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATACCCGGCTCGGGAACTTGCAGAGCTGCGTTTGa                                                     >  2:1015492/1‑300 (MQ=255)
                                                                                                                                                                                      ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAAt                                                  >  2:724872/1‑301 (MQ=255)
                                                                                                                                                                                       cAGACGGGGCAATGGCCTGATTTAGCGGGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATa                                                 <  1:214642/301‑1 (MQ=255)
                                                                                                                                                                                          acaggggaatatcctgttttgtgctttgtttGTAGCTCGTATAAGGCTCTTTC‑C‑‑CCGCTGCCGTCATCAATCCCTGATGCGACGCTTGCCGCGTCTTATCACGCCTATCTTAACCGTTTGTTAATTTTTCAAACTCTTCTCACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCATCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCa                                              <  1:321528/273‑1 (MQ=255)
                                                                                                                                                                                          aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCa                                              <  2:129898/301‑1 (MQ=255)
                                                                                                                                                                                           cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAAACGGTTCGGGAAATTGCAGCGCTGCGTTTGAAATAcc                                               >  2:1112567/1‑299 (MQ=255)
                                                                                                                                                                                             gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑CCTCGGAATAACCGGTTCGGGAAATTGCAGTGCTGCGTTTGAAATACCAGGt                                           >  1:623768/1‑301 (MQ=255)
                                                                                                                                                                                                           tttAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAtcg                              <  2:517444/300‑1 (MQ=255)
                                                                                                                                                                                                              ggtgtgtTTTTGTAGGTCGGATCAGGC‑GTTTCCG‑‑CCGCATCCTACATCAATGCCTTATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCgg                          <  1:1190269/298‑1 (MQ=255)
                                                                                                                                                                                                              aGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCgg                          >  2:789673/1‑301 (MQ=255)
                                                                                                                                                                                                               gCGTGATTTTGTAGGTCGGATAAGGC‑TTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTTTCCGTTGGTTTATTTTTCAAACTCTTCACCACCGGTATCAAACCCTTCCATACATCTCAG‑GTTCAACCAGTGCACCACCTTTTGCTTGTTCTTCTCGCTAT‑AACCTTCTTCACCGCTTCAGCACATCCTTCCCGCCCCGT‑CCTCGGTATACCCGGTTCGGGAAATTGCCTCGCCGCTTTTTAAATCCCCGGTTGCCTTGTCCTCTTCGGc                         >  2:454085/1‑301 (MQ=25)
                                                                                                                                                                                                               gCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAACTTGCAGAGCTGCGTTGGAAATACCAGGTTGCCTTGTCATCGTCGGc                         >  1:1187930/1‑301 (MQ=255)
                                                                                                                                                                                                                      tttGTAGGTCGGATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAAt                   <  2:602250/300‑1 (MQ=255)
                                                                                                                                                                                                                      tctGTATGTCGGATCAGTC‑GTTT‑‑ACGCCTCATACGACATCAATACGTGATGCGACGCTTGCAGCGTCTTATCAAGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCGTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAAt                   <  1:1112273/298‑1 (MQ=255)
                                                                                                                                                                                                                                ggATAAGGC‑GTTTCCG‑‑CCGCTTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCgg                          <  2:1243082/283‑1 (MQ=255)
                                                                                                                                                                                                                                ggATAAGGC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGTTTGCCTTGTCATCGTCgg                          >  1:1242748/1‑283 (MQ=255)
                                                                                                                                                                                                                                    aaGGC‑GTTT‑‑ACCCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTACCCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTgg     <  1:384358/300‑1 (MQ=255)
                                                                                                                                                                                                                                      ggC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACCGCCCAG‑GTTCAACCCTTGCAGCGCCTTTTGCTTTTTCTCCTCGATAA‑AACCTTCTTAACCGTTTACGAAAACCATTCCCGCCCAGT‑ACTCGGAATAACCGTTTCGGGAAATTCAAAAGCTGCCTTTCAAATACCAGGTCGCTTGGTCACGGTCGGCCTGAATTccacccctgttcgggt  >  1:219440/1‑287 (MQ=255)
                                                                                                                                                                                                                                      ggC‑GTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGCCGt  >  2:1093659/1‑301 (MQ=255)
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                                                                      
TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTG‑TAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAG‑GTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAA‑AACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGT‑ACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGT  >  NC_000913/4296144‑4296671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑