mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | SSW-KHP-8-91-9_S27_L001_R2_001 | 834,104 | 250,777,003 | 100.0% | 300.7 bases | 301 bases | 62.7% |
| errors | SSW-KHP-8-91-9_S27_L001_R1_001 | 834,112 | 250,670,943 | 100.0% | 300.5 bases | 301 bases | 64.2% |
| total | 1,668,216 | 501,447,946 | 100.0% | 300.6 bases | 301 bases | 63.4% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 67.7 | 2.1 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 6622 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 15 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.002 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.91367 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.8 |
| Consensus minimum coverage each strand | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum coverage each strand | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 22:58:14 16 Apr 2016 | 22:59:07 16 Apr 2016 | 53 seconds |
| Read alignment to reference genome | 22:59:07 16 Apr 2016 | 23:03:21 16 Apr 2016 | 4 minutes 14 seconds |
| Preprocessing alignments for candidate junction identification | 23:03:21 16 Apr 2016 | 23:04:08 16 Apr 2016 | 47 seconds |
| Preliminary analysis of coverage distribution | 23:04:08 16 Apr 2016 | 23:05:51 16 Apr 2016 | 1 minute 43 seconds |
| Identifying junction candidates | 23:05:51 16 Apr 2016 | 23:05:53 16 Apr 2016 | 2 seconds |
| Re-alignment to junction candidates | 23:05:53 16 Apr 2016 | 23:06:15 16 Apr 2016 | 22 seconds |
| Resolving alignments with junction candidates | 23:06:15 16 Apr 2016 | 23:07:51 16 Apr 2016 | 1 minute 36 seconds |
| Creating BAM files | 23:07:51 16 Apr 2016 | 23:08:52 16 Apr 2016 | 1 minute 1 second |
| Tabulating error counts | 23:08:52 16 Apr 2016 | 23:10:36 16 Apr 2016 | 1 minute 44 seconds |
| Re-calibrating base error rates | 23:10:36 16 Apr 2016 | 23:10:37 16 Apr 2016 | 1 second |
| Examining read alignment evidence | 23:10:37 16 Apr 2016 | 23:23:15 16 Apr 2016 | 12 minutes 38 seconds |
| Polymorphism statistics | 23:23:15 16 Apr 2016 | 23:23:15 16 Apr 2016 | 0 seconds |
| Output | 23:23:15 16 Apr 2016 | 23:23:36 16 Apr 2016 | 21 seconds |
| Total | 25 minutes 22 seconds | ||
