| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 781,757 | G→T | intergenic (+105/‑328) | lysQ → / → nadA | tRNA‑Lys/quinolinate synthase, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 781,757 | 0 | G | T | 97.6% | 120.4 / ‑5.5 | 42 | intergenic (+105/‑328) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (19/22); minor base A (0/1); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGT > NC_000913/781461‑782051 | tggtgaggaACGGGAAGGATAAGGTTGCGTTAGCAACGGCACGAAGGGCGAGACGAAGTGGAGTCATCCTGTACGACCCACCATCCTGAATGAGTAGGGCAGCATAATCCCGGAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCtcgg < 2:566132/293‑1 (MQ=18) aCGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCg > 1:411165/1‑301 (MQ=255) ttGCGTAAGCAACGGCCCGAAGGGCGAAACGAAGACGAGCCAACCTGCACGAACCACCATCCTGAATGATTAAGGCAGAATAAGCCCGCAAGGGGTAGTTAGCTCCGTTGGGAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAGAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGGTACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGagcgaagc < 2:628539/301‑1 (MQ=18) aaCGGCCCGAAGGGCGAGACGAAGACGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCt < 2:395879/301‑1 (MQ=255) aCGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATc > 1:803/1‑298 (MQ=255) aCGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGAAAGGTGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATg < 2:650997/299‑1 (MQ=255) tCGAGTCATCCTGCACGACCCACCATACTGATTGAGTAAGGCAGCAGAATTCCGAAAGGGTGCGTTAGCTAAGTTGGTAGTGCAGTTGAATTTTAAGCAATTGGTCGCAGGTTCGAATCCTGAACGACCAACGAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGGTGAGCGCAGCGAAAAAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAg < 2:176764/301‑1 (MQ=14) cATCCTGCACGACCCACCATCTGGAATGATTAAGGCAACATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATGGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGAGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCACGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCt < 2:64701/301‑1 (MQ=255) ccTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTTCACGACCCACCAATGTAAAAAAGCGCCCTaaa > 1:541955/1‑301 (MQ=255) acaaaccagtcaaccTGAATGATTAAGGCAGCAAAATCCCGCAAGGGGTCGTAGGCACATTTGGAAGAGCAGTGGACTGGTAATCAATTGGTCGCAGGTTCGAAGCCTGCACGACAAACCAAGGTAAAAAAGCGCCCTAAAGGGGCTTTTTGGCGATCGGAGATACTCAAAGATTCGAACCTGAAGCAGGTTTGAGTGGAGCGCAGCGAAAGAACGGAGCCGCTCTCGGGGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc < 2:411161/287‑1 (MQ=14) ccaccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAGGCGCttttt > 1:594448/1‑301 (MQ=255) aaTGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGACGGGCGAGCGAAGCGAGTCATACTGCACGACCCACCACTGTAAAACAACGCCCTAAAGGCGCTTTTTTGCTCTTCAgg > 2:189092/1‑301 (MQ=255) gcagcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAAt > 1:688341/1‑301 (MQ=255) aTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGACGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCTTCAATTTCa > 1:151426/1‑300 (MQ=255) aTAAGCCCGCATGTGGTCGTTAGATCCGATGTTAATGAAGTTGAATTTTAATCACTTTGTGGCAGGTTCGAATCCTGCACGACCCACCACTGTAAAAAAGCGCCTTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCATGTTTGAGTTGAGGGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCAc < 2:488382/301‑1 (MQ=14) ccccGCAAGGGGTCGTTAGCGCAGTTGGTAGGGCAGTTGAATTTTAAACAGTTGGTCGCAGGTTCGAATACTGCACGACCAACCAATGTAAGAAAGCGCCCTAAAGTCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTg < 2:306115/300‑1 (MQ=255) gTCGCTAGCTCAGTTGGTAGAGCAGTTGAATTTTAATCACTAGGTCGCCGGGTCGATTCCTGCACGACACACCAATGTAAAAAAGCGCCCTAAAGACGCTTTTTTGCTATCTGCGATGCTCAACGATTCGAACCGGCAGCAGGTTTGAGTTGGGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgat < 2:616136/300‑1 (MQ=14) gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGACGGGCGAGCGACGCGAGTCATCCTGCACGACCCACCAATGTCAGAAAGCGCACTCAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACa > 2:652172/1‑301 (MQ=255) tAGCTAAGTTGTTAAAGAAGTTGACATTTGATCAATGGGGCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATc < 2:151424/301‑1 (MQ=255) cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTc < 1:644046/301‑1 (MQ=255) tGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCagag > 1:638942/1‑299 (MQ=255) gCAGTTGACTTTTAATCAATCGGTCGCAGGTTCGAATCCCGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCCATCCGCGATACTCAAAGACTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGCGCGAAGCGAGTCCTCCTGCCCGACCCATCAATGTAAAACAGCCCTCTAACGGCGCTTTTTTGCTATTCACGCATCCTCAATTTCACTTTGTAAATCTGTTTACATTGTCAGTTCTTACtctt > 2:466197/1‑298 (MQ=14) cAGTTGACTTTTAATCAATTGGTCGCAAGTTCGAATCCTGCACGACCCACCACTGTAAAAAAGCGACCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACtgtg < 2:638935/300‑1 (MQ=255) ttGACATTAAATGAATTGGTGGAAGGATCGAAGCCTGCAGGACACACAAATGTAAAAAAGCGCCCTAAGGAGGCTTTGTTGCTAAAGGCGCGACTAAAGGATTCAAACCGCCAGAAGGTTAGAGTGGAGGGAAGCGAAAAAACGGAGGCGCTCTGGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaag < 2:4196/301‑1 (MQ=17) ctcttccgatctccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATg < 1:322046/288‑1 (MQ=255) ccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGagatcggaagagg > 2:322043/1‑289 (MQ=255) tAAAAAAGCGACTTACAGGCTCTGTTTTGCTATCTGCGATACTCACAGATTCGAACCTTCAGCAGGTTTGATTTAGGCGCCGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGATCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAg < 2:541950/300‑1 (MQ=21) ctgccgtttgctcttccgatctCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAg < 1:32629/280‑1 (MQ=255) tttttGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGATTGACTTTTccc > 2:66071/1‑300 (MQ=255) cTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCgagagatcggaagagcgtcgtgtag > 2:32629/1‑282 (MQ=255) tGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGa > 1:264411/1‑299 (MQ=255) cAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGttt > 2:72306/1‑301 (MQ=255) aaaaGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGttt < 1:189094/300‑1 (MQ=255) tgcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAAtt < 1:357319/300‑1 (MQ=255) agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAAt < 1:72306/300‑1 (MQ=255) gcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTa > 2:357316/1‑301 (MQ=255) ccccttccgatctgcAGCGAAACAACGGAGCCGCCCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACAcc < 2:388480/288‑1 (MQ=255) tttGAGATGGACGAAGCGAAACAACGGAGCCGGTCTCGGCGACGTCCCGAAGGGCGAGGGAAGCGAGTCATCCTGCAAGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAGACCTGATGACGTCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACAcc < 2:594442/300‑1 (MQ=38) ttgagCGCAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACACCGATAAc < 1:66071/300‑1 (MQ=255) gcAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACAccagatcggaagag > 1:388483/1‑288 (MQ=255) gcAGCGAAACAACGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGC‑TTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACACCGATAACAGCGAata > 2:164100/1‑301 (MQ=255) aacaacGGAGCCGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGTGCTTACTGTGCAAGCAACTCTATGTCGGTGGAACTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACACCGATAACAGCGAATATTACGc < 1:466202/301‑1 (MQ=255) ccGCTCTCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGt > 2:167518/1‑301 (MQ=255) | TGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTCCCCACCATTCGACTATCTTGTTTAGCATATAAAACAAATTACACCGATAACAGCGAATATTACGCTAATGTCGGT > NC_000913/781461‑782051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |