Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	15,601	0	G→C	100.0%	0.0	1	G53R (GGG→CGG)	insL1	IS186 transposase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (1/0):  ref base (0/0):  total (1/0)

GCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAA‑CTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTAC‑GGCCCCGGGGGGATGTCATTA  >  NC_000913/15358‑15615                                                                                                                                                                                                                                                      |               gCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAA‑CTTAAAGGGTGTGGGATTTCGCCCGATATTATTTTAAGTGCCGGTGAATTACTCTTACGATAAATGGGTAGCAATTCTGGCCCATATTGGTAAACCCGAAGAACTTGATAATTCGGCACGTAAAGCCGGGGCTCTAAACCGCCGCCCCGAAAATCGTGATGCTGCAACTCTGCTACATCTGGGGGCGGCGTACAGGCCCCggggggg          >  1:3009312/1‑250 (MQ=14)        ccTGTAAAGTGGCGGGGGTCACCCCCCTAAACGCCAACCTTAAGGG‑TGTGGGATTACGCCCGATATGATTTTAAGTGCCGGTGGATTACTCTCACGATAACTGGTCAGCAAATCTTGCCCCTATTTGTAAGGCCGGAGAAATGGAAACTTTCGCACGGAATGCCGGGGGTCTAAACCGCCGCCCCCAAAATCGTGCTGCTGCAAACCATCTACCGCCGGGGGGTGCTTGC‑GGCCCCCGGGGGGTgggctta  >  2:3208714/1‑245 (MQ=2)        ccTGTAAAGTGGCGGGGATCACCCCCATAAGCGCTAA‑CTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTAC‑GGCCCCggggg            <  1:5586750/241‑1 (MQ=38)                                                                                                                                                                                                                                                      |               GCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAA‑CTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTAC‑GGCCCCGGGGGGATGTCATTA  >  NC_000913/15358‑15615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.