Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009131,410,1231.→T 1.0% 0.9 0.0 ‑0.1200coding (613/1374 nt)dbpAATP‑dependent RNA helicase, specific for 23S rRNA
Rejected: E-value exceeds prediction threshold.
Rejected: Polymorphism does not pass frequency cutoff.
Rejected: Polymorphism prediction not supported by minimum number of reads on both strands.
Reads supporting (aligned to +/- strand):  new base (1/1):  ref base (97/101):  total (98/102)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.15e-01

Only 100 of 201 total aligned reads displayed.

GTTGTGCGGTGGTCAACCGTTCGGTATGCAGCGTGATTCGTTGCAACATGCGCCGCATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTCGCGACTGATGTTGCTG  >  NC_000913/1409825‑1410414
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
gTTGTGCGGTGGTCAACCGTTCGGTATGCAGCGTGATTCGTTGCAACATGCGCCGCATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATCCGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGTCCTGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGAtt                                                                                                                                                                                                                                                                                                   >  2:1285117/1‑301 (MQ=255)
       ggggggcAACCGGTCGGTAGTGGGCGTGATTCGGTGCAACATGCGCCGCATAGTATAGTGGAAACGCCGGGGCGTTGGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAAtt                                                                                                                                                                                                                                                                                             <  1:846488/294‑1 (MQ=255)
        gtggtCAACCGTTCGGTATGCAGCGTGATTCGTTGCAACATGCGCCGCATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCAGTTGAAATTGa                                                                                                                                                                                                                                                                                           >  2:465749/1‑301 (MQ=255)
          ggtCAACCGTTCGGTATGCAGGGTGAATCGTTGCAACATGCGCCGCATATTCTCGGGGCAACGCCGGGGGTTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGAGATGGGATGTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGAc                                                                                                                                                                                                                                                                                          <  2:1323322/300‑1 (MQ=255)
                    tCGGTATGCAGCGTGATTCGTTGCAACATGCGCCGCATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCATGCATCTCGACAGACGCTTATGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACCGacgg                                                                                                                                                                                                                                                                               >  2:593437/1‑298 (MQ=255)
                          tGCCGCGTGATTCGTTGCAACATGCGCCGCATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGcc                                                                                                                                                                                                                                                                         <  2:506216/301‑1 (MQ=255)
                                   aTTCGTTGCAACATGCGCCGCATATTATCGTGGCAACGCCGGGGCGGTTGCTGGCTCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGCGATGGATGCGGCCGAACGCATGCTGGATAAGGGCTTAAGGGATGCCATTGATGATGTCATCAGTTTTGCGCCTGCATCTCGAAAGACGCTTCTGTTTTCGGGAACTGCGCCGGAACACCTCGCTGCAAGCAGCAGCCGAGTGCCACGCGGTGCTTGGGCGATTGAAATTGACGCACCAgcagatggtccacacacaaa                                                                                                                                                                                                                                                                >  1:950628/1‑282 (MQ=255)
                                          gCAACATGCGCCGCATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCCACCTGGCCGGACGCCATCGCTGCAATCAGCGGCCGAGTGCAACGCGATCCTTTGGCGCTTGCAATTGACCCACACGCTCCTTTGCCACCCATTGaacacaca                                                                                                                                                                                                                                                         >  1:938365/1‑297 (MQ=255)
                                                 gcgcCGCATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGCTCCTTTGGCGATTGAAATTGACTCAACCGATCCTTTGCCACCCATTGAACAACACTTTTATg                                                                                                                                                                                                                                                  >  1:465985/1‑301 (MQ=255)
                                                    ccgcATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGCGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCCTTGAACCACACTTTTATgaga                                                                                                                                                                                                                                               >  1:90091/1‑301 (MQ=255)
                                                      gcATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTTATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGCCGCTTCTGTTTTCGGCAACCTGGCCGGACGCCATCGCTGCAATCAGCGGACGGGTGCAAGCCGATCCTTTGGCGATTTAAATTGACTCAACACATGCTTTCCCCCCCCTTTACACCCAATTTTAttcaaaa                                                                                                                                                                                                                                             >  1:1256386/1‑295 (MQ=255)
                                                      gcATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGCAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGCTCCTTTGGCGCTTGAAATTGACCCAACAGATGCTTTGCCCCCCATTGAACAGCAAGTTTATGAGACa                                                                                                                                                                                                                                             >  2:467456/1‑301 (MQ=255)
                                                        atatTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGTCATTGCTGCTGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGCAGCCATCGCTCCCATCAGCGGACGAGTGCACCGCGCACCTTTGGCGATTGACATTGACTCAACAGATGCTTTTCCACCCATTGAACAACAATTTTATgcaccata                                                                                                                                                                                                                                           >  1:962136/1‑294 (MQ=255)
                                                        atatTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGTCGATTGAAATTGCCTCAACCGATGCTTTGCCACCCATTGAACAACATTTTTATGAGCCAt                                                                                                                                                                                                                                            >  2:1188532/1‑300 (MQ=255)
                                                                 tGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGACCCAGTGCAACGCGATCCTTTGGCCATTGACATTGCATCAACCGATGCTTTGCCACCCCTTAAACACCCATTTTATGAGACCTCCaaacacga                                                                                                                                                                                                                                  >  1:1068784/1‑294 (MQ=255)
                                                                        gCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGGGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTAAACAGATGCTTTGACACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGAaaaaat                                                                                                                                                                                                                           >  2:114105/1‑299 (MQ=255)
                                                                                       ggATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATACTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTTGCAAACCATTTTATGAGAACTTCAGCAAAGGCAAAATTCCTCTGTTGCaa                                                                                                                                                                                                               >  1:447049/1‑298 (MQ=255)
                                                                                        ggggagggttataagagacaggTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGAGGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACgg                                                                                                                                                                                                            <  2:639349/280‑1 (MQ=255)
                                                                                             ccTGAAAAAAGGCACGGTAGTACGGGGGTGGGGT‑AATACGCGTGTTATGGATGAAGCCGCACGCATGAGGGATAGGGGCTTGAGCGATGCCATTGATGAGGTCATACGTTGTGCGCCGGGAGCTAGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCGGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTTAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGttatta                                                                                                                                                                                                      <  1:585446/301‑1 (MQ=255)
                                                                                                gaaaaaaGGCACGGTATAAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAg                                                                                                                                                                                                    <  2:1102734/298‑1 (MQ=255)
                                                                                                   aaaaGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTTAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTCCAACGGTTATTAAGCt                                                                                                                                                                                                  >  1:1235074/1‑299 (MQ=255)
                                                                                                        gCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATc                                                                                                                                                                                            <  2:1224693/300‑1 (MQ=255)
                                                                                                           cGGTATCAT‑TGGATGCGTTGAATACGCTGGTGATGTATTATGCCGACCGCATGCTGGATATGGGATTTAGCGATGACATTGATGATGTCATCCGTTTTGTGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTTGCCGGAAGCCATCGCTTCAATCAGCGGACTAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGcc                                                                                                                                                                                        <  2:1313546/301‑1 (MQ=255)
                                                                                                           cGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCACTCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAACTTGCCTCAACAGATGCTTTGCCACCCATTGAACCACACTTTTATGCTACATCCAGCCAAGGCAAAATTCCTCTGTTGCCACGGTTATTAAGCTTGCCTCAGc                                                                                                                                                                                         >  2:940761/1‑300 (MQ=255)
                                                                                                             gTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGCCAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGCCGAGTGCAACGCGAGCCTTTGGCGATTGAACTTGACTCAACAGATGCTTTGCCACCCATTGACCAACAATTTTATGAGACATCCAGACAAGTCAAAATTACTCTGTTGCAACGGTTATTAAGCTTCCACCAGCCaa                                                                                                                                                                                      >  1:942848/1‑300 (MQ=255)
                                                                                                             gTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGaac                                                                                                                                                                                                                                                              <  2:871402/229‑1 (MQ=255)
                                                                                                             gTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGCACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCAt                                                                                                                                                                                      >  2:1368396/1‑301 (MQ=255)
                                                                                                             gTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGACCAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCAt                                                                                                                                                                                      >  2:1379557/1‑301 (MQ=255)
                                                                                                             gTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGCCATCCAGCAAAGGCAAAATTCCTCTGTTGCCACGTTTATTACGCTTGCATCAGcaaa                                                                                                                                                                                      >  1:215894/1‑298 (MQ=255)
                                                                                                             gTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCCACGGCAAAATTCCTCTTTTGCAAAGGTTATTAAGCTTGCATACGCCaa                                                                                                                                                                                      >  2:1211196/1‑300 (MQ=255)
                                                                                                             gTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAAACACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCa                                                                                                                                                                                       >  1:619440/1‑300 (MQ=255)
                                                                                                                 cAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACCACAATTTTATGAGACATCCCGCAAAGGCAAATTTACTCTGTTCCAACGGTTATTAACATTGccttcaccctccc                                                                                                                                                                                   >  1:60717/1‑288 (MQ=255)
                                                                                                                 cAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATga                                                                                                                                                                                                                                                 >  2:76568/1‑238 (MQ=255)
                                                                                                                 cAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATga                                                                                                                                                                                                                                                 <  1:76568/238‑1 (MQ=255)
                                                                                                                 cAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGAATCAACAGATGCTTTGCCACCCATTGAAAAACAAt                                                                                                                                                                                                                                                        >  2:338215/1‑231 (MQ=255)
                                                                                                                 cAC‑GGGATGCGTTGAAGACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAAt                                                                                                                                                                                                                                                        <  1:338213/231‑1 (MQ=255)
                                                                                                                   c‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGACATTGAGGATGTCATCCGTTTTGCGCCTGCATCTCGACAGAATCTTCTGTTTTCAGCAACCTGGCCGGAAGCCAACGCTGCACTAAGCGGACGAGTGAAACTCGATCCTTTAGCCATTGAAATTGACTCAACAGCTGCTTTGCCACCCCATACACAACCATTTTATGAGACCTCCAGCGACGACAAAATTCCTGCTTTCCAACGGTTATTAACCTTTCATCCTACATCCTCtt                                                                                                                                                                                >  2:1100860/1‑301 (MQ=255)
                                                                                                                     tgtgtGCGTTGAATTCGCTGGGAATAGAGGAGGCCGGCCGCATGGTTGGTATGGGTTTTAGCGAGGCAATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGATGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATGGACTCAACAGATGCTTGGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCct                                                                                                                                                                                   <  1:1285087/293‑1 (MQ=255)
                                                                                                                     tGGGTGCGTTGAATACGCGGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTg                                                                                                                                                                               <  1:1368364/301‑1 (MQ=255)
                                                                                                                        aTGGGTTGAATACGCTGGTGAGGGATGAGGCCGACCTCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCg                                                                                                                                                                             <  1:1379524/300‑1 (MQ=255)
                                                                                                                        aTGCGTTGAATACGGTGGTGAGTGAGGAGGCCGACCGCATGCTGGATAGGGGATTTAGCGAGGCCATTGATGATGTCATCCGGTTTGCGACTGCAGCGCGACAGACGCGTCTGTTTTCGGCAACCTGGACGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCgt                                                                                                                                                                            <  2:4488/301‑1 (MQ=255)
                                                                                                                          gCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCATTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCAACCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTACTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCgtgg                                                                                                                                                                          >  2:278826/1‑301 (MQ=255)
                                                                                                                             ttGAATACGGTGGTGATGGATGAGGCCGAACGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGGCATCCGTGTTGCGACTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGGTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGgtgt                                                                                                                                                                       <  2:585205/301‑1 (MQ=255)
                                                                                                                             ttGAATACGCTGGTGGTTGATTCTGCCGACCGCATGCTGGATATGGGATTTAGCGCTGCCATTTATTATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGGTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGgtgt                                                                                                                                                                       <  1:706172/301‑1 (MQ=255)
                                                                                                                                  tgagcggttggtggaggGGGCCGACGGTATTCTGGATATGGGATTTAGCGATGCACTTGATGATGGCATCCGGTTTGAGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGAAACTCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGc                                                                                                                                                                  <  1:1138849/290‑1 (MQ=255)
                                                                                                                                  tactcgtggggtgggtgACGGCGGCCGCATGCTGGATATGGGATTTAGGGATGCCATTGATGATGGCATCCGTTTGGCTCCTGCATCTCGACAGACGCTTCTGGTGTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAAGTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAACGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGc                                                                                                                                                                  <  1:357257/290‑1 (MQ=255)
                                                                                                                                   aCGCTGGTGATGGCTGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCCATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGCAATCCAGCCAAGGCAAAATTCCTCTGTTTCAACGGTTATTCAGCTTTCCACAGCCATCCTCTTGCGTGGGTTTTTGCa                                                                                                                                                                 >  1:666088/1‑301 (MQ=255)
                                                                                                                                              ggatgAGGGCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCAGCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCAt                                                                                                                                                                                                                                                                   <  2:1054204/192‑1 (MQ=255)
                                                                                                                                                 tgAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCTCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGAt                                                                                                                                                   <  2:613474/301‑1 (MQ=255)
                                                                                                                                                    ggCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCCATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGCAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGTTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTg                                                                                                                                                 >  2:129739/1‑300 (MQ=255)
                                                                                                                                                         aCCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCCCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGCACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCCAAAAAGATTGCCAgg                                                                                                                                            >  1:40727/1‑300 (MQ=255)
                                                                                                                                                               tgctcgttattgtgtttAGTCATGCCAGTGTTGCTGTCATCCGTTTTGCGCCTGCACCTCGACAGACGCTGCTGTTTTCGGCAACCTGGCCGGAGTCTATCTCTGCAATCATCGCACCAGTGCAACCCGATCCTTTGGCGATTGAAAGTGTCGCCACAGATGCTTTGCCACCCATTGACTTACAATTCTACGAGACATCCCGCAAAGTCAACATTCCTCTGTTGCAACGTTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTg                                                                                                                                     <  2:172267/287‑1 (MQ=255)
                                                                                                                                                                 actgATATGTGATTTCGCGAAGCCTGGTATGAGGTCTTTCGTTTTGCGCATGCATCGCGACAGACGCCTCTTGTTTCGGCAACCTGGCCGGAAGCCATAGCTTCAATCAGCTGACGCGGGCAACGCGATCCTTTGGCGCTTGAAATTGACTCAACCGATGCCTCGTCACCCACTGAACAACAATTTTATGAGACCTCCAGCAAAGGCAAAATTCCCCTGTTGCAACGGTTATTAAGCGTGCATCAGCCATCCTCTGGCGTGTTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGc                                                                                                                                    <  2:892884/297‑1 (MQ=255)
                                                                                                                                                                   ggATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAg                                                                                                                                                                                                    >  2:1330269/1‑234 (MQ=255)
                                                                                                                                                                   ggATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCAGCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAAGAGAGACTTTGCCACCCATTGAACAACAATTTTATGAGCCATCCAGCAAAGGAAAAATTCCTATGTTGCAACGGTTATTAGACTAGCATCACCCATGCCCTTGAGTGGTGTTTTCAAAGACAAAAAAAGTTGTCCAGGCTGCCTGcaag                                                                                                                                 >  1:1018432/1‑298 (MQ=255)
                                                                                                                                                                    gatcggggATTTGACGATGCCATTGAGGAGGTGATACCGTTTGCGCCGGCAGCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGAc                                                                                                                                 <  1:129739/295‑1 (MQ=255)
                                                                                                                                                                     atatGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTTGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCACCGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGATGTTTTGCAATCCCAAAAAAGATTGCCAGGCTGTCTGCGTcgg                                                                                                                               >  2:694655/1‑300 (MQ=255)
                                                                                                                                                                     atatGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATATCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAg                                                                                                                                                                                                    <  1:743796/232‑1 (MQ=255)
                                                                                                                                                                     atatGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTACGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACCTCCAGCACAGGCAAAATTCCTCTGTTGCAACGGTTCTTACGCTTGCATCatac                                                                                                                                                                                        >  1:1077008/1‑241 (MQ=255)
                                                                                                                                                                          ggATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAGCAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCCTCCTCTTGCGTGGTATTTTGCAATACCAAAACAGTTTGCCAGGCTGACTGCGACGCGCtga                                                                                                                          >  1:314057/1‑301 (MQ=255)
                                                                                                                                                                             tttgtcGAGGCCATTGAGGATGTATGCCGGGTGGCGCCTGCATAGCGACAGACGTGTCTGGTTTCGGCAACTTGTCCGGAAGCCATCGCTGCAATCAGCGGACTAGGGCAAAGCGAGCCTGTGGCGACTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCGGTTGCAACGCTTATTAAGCTTGCATCAGCCATCCTCTTGCTTGGTGTTTTGCAATACCAAAAAAGATTTCCAGGCTGTCTGAGACGCTCtgaagg                                                                                                                       <  1:1061738/296‑3 (MQ=255)
                                                                                                                                                                                 gggaggCCATTGAGTGTGGCAGCCGTTTGGCGCCTGGATCGCGACAGACGGTGCTTTTTTGGGCAACCTGGACGGGAGCCATGGCTGCAAGCAGTGGACGAGTGCAACGCGATCCTGTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAg                                                                                                                    <  1:37664/295‑1 (MQ=255)
                                                                                                                                                                                 cctatgacatggtgtaggTCATCCGTTTGCCCCCGGCAGCACGACAGCAGCTTCTGTTCTCGGCAACCAGGCCGGAAGCCATCGCTGCGAGCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCAGCCATTGACCAACAATTTTATGAGACATCCAGCAAAGGCGAAATTCCTCTGTTGCAACGGCTATTAACATTGCATCAGCCATCCCCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTCCGACGCGCGGAATGAAGt                                                                                                                   <  1:997659/284‑1 (MQ=255)
                                                                                                                                                                                  cGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGt                                                                                                                   <  2:679932/300‑1 (MQ=255)
                                                                                                                                                                                             atgatggaatcaggttgtcgcATGCATCTGGACAGACGCTTCTGTTTTAGGCAACCTGGACGGCAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCATTTGGAGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACCCCAATTTTATGAGACATCCAGCACAGGCACAATTCATCTGTTGCAACGTTTATTAACCCTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACGAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTg                                                                                                        <  2:789077/282‑1 (MQ=255)
                                                                                                                                                                                               gatgTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACgg                                                                                                                                                                                                            <  1:494535/198‑1 (MQ=255)
                                                                                                                                                                                                atgTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGCATGAAGTAGGGCAAAGTGCAtt                                                                                                    >  2:849074/1‑301 (MQ=255)
                                                                                                                                                                                                   tCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGt                                                                                                  <  2:357464/300‑1 (MQ=255)
                                                                                                                                                                                                   tCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTc                                                                                                 <  2:754342/301‑1 (MQ=255)
                                                                                                                                                                                                    cATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCACAATTCCTCTGTTGCAACGGTTATTACGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTTCGACGCGCTGAATGAAGTAGGGCCAAGTGCATTGTc                                                                                                 >  2:946234/1‑300 (MQ=255)
                                                                                                                                                                                                    cATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCCGGCTGTCTGCGACGCGCtgaatctgtctcttctacacatttccga                                                                                                 >  2:951095/1‑277 (MQ=255)
                                                                                                                                                                                                    cATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCa                                                                                                >  2:383685/1‑301 (MQ=255)
                                                                                                                                                                                                        tgTTTTG‑GCGTGCATCTCGACAGACGCTTCTGTTGTCGGCAACCGGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTaca                                                                                           <  1:132366/300‑1 (MQ=255)
                                                                                                                                                                                                          cgcggcggcagaggtgtttaagggacagcTGTTTTCGGAAACCTGGCCGGAAGCCATCGCTGCAATCTGCGGACGAGTGCATCGCTATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCAGCAGCCATCCTCTTGCGTGGTGTTGTGCAATACCAAAAAAGAGTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTacac                                                                                          <  1:1267697/273‑1 (MQ=255)
                                                                                                                                                                                                                     cATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCAt                                                                                                                                                                                                                                                                   <  2:337001/121‑1 (MQ=255)
                                                                                                                                                                                                                     cATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCaa                                                                                                                                                                >  2:270431/1‑220 (MQ=255)
                                                                                                                                                                                                                     cATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCaa                                                                                                                                                                <  1:270430/220‑1 (MQ=255)
                                                                                                                                                                                                                       tctcGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCGGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGc                                                                             <  2:918207/301‑1 (MQ=255)
                                                                                                                                                                                                                          cGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGCCGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAACGGCAAAATTCCTCTGTTGCCACGGTTATTAAGCTTGCATCATCCATCCTCTCGCGTGGTGTTTTGCAATACCAAAAAAGGTTGCCCGGCTGTCTGCGACGCGCTGACTGAATTAGGGCAAAGTGCCTTGTCCTTACACGGCGGTTTGGCGCac                                                                           >  2:844554/1‑299 (MQ=255)
                                                                                                                                                                                                                                   cgtcggtgttCGGCAACCGGGACGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCGTTGGCGAGTGAAATTGACTCAACAGATGCGTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAAcgcgatcgc                                                                  <  2:768327/292‑1 (MQ=255)
                                                                                                                                                                                                                                      tcgTTTGCGGCAACCGCGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGTGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTGTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCAGCAGCCATCCTCTTGCGTGGTGTTTGGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACgcgatcgcgatc                                                              <  2:654701/299‑1 (MQ=255)
                                                                                                                                                                                                                                      cTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTTCGACGCGCTGAATTAAGTAGGGCAAAGTGCATTGTCATTacac                                                                                          >  2:1267727/1‑273 (MQ=255)
                                                                                                                                                                                                                                            tCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAAAAGAGGCTTTGCCACCCATTGAACAACAAGTTTATGAGACAGCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGAccc                                                        <  2:1237597/301‑1 (MQ=255)
                                                                                                                                                                                                                                                  cccTGGCCGGAAGCCATCGCTGCAATCAGCGGACTAGTGCAACGCGATCCTTTGGCGATTGAAAGTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTCCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTAc                                                  <  1:278825/300‑1 (MQ=255)
                                                                                                                                                                                                                                                            aaGCCATCGCTGCAACCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTGGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTa                                         <  2:743158/300‑1 (MQ=255)
                                                                                                                                                                                                                                                               ccAGCGCTTCAATCAGCGGACGAGTGCAGCGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACgg                                     <  1:681437/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tCGCTGCAATCAGCGGACGATTGCAACGCGATCCTTTGGCGATTTAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAg                                  <  1:1211167/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAAGGCAAAGTGCATTGTCATTACACGGCGATTTGGCGCAACGCGATCGCGATCAGACCCTGGTTCGTTTTGCTAAAGGTAgca                                >  1:645196/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                             cgcggagcagggaaaCGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTAc                       <  1:1151149/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGcc                              <  2:351353/293‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGcc                              >  1:351351/1‑293 (MQ=255)
                                                                                                                                                                                                                                                                               cGGACGAGTGCAACGCGATCCTTTTGCGAGTGAAATTGACGCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGAAAAATTCCGCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTAc                       <  2:1068811/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cGGAAGAGTGAAACGCGATCCTTTGGCGATTTAAATTGAATCAACAGATGCTTTGCCACCCAGTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTGTTGCAATACCAAAAAAGATTGCCAGGCTGTCGGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTAc                       <  1:780245/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 gaagAGTGCAACGCGATCCTGTGGCGATTGAAATGGACTCAACATATGCTGTGTCACCCATTGAACAACAATTTGATGAGACATCCAGCAAAGGCACAATGCCTCGGTTGCAACGGTTATTAAGCTGGCATCAGCCACCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTACTgg                    <  1:593429/297‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  aCGAGGGCAACGCGGTCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACAGCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTc                  <  1:992448/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    gAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACgg                                                                                        <  1:891647/229‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           aCGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCACAGGCAAACTTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATCCCACAAAAGATTGCCAGGCTGTCTGCGACGCGCTGCATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGATCACCGCGATCGCGATCATCCCCTGGTCCGTTTTGCTCACTGTACCCCCCGTGTCCTGGTCGCGCCTTAt         >  2:978431/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                aTCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCATTACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTCGCGCCTGATGTtg     >  1:860149/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  ccTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCaa                                                                                                                                                                <  2:220341/143‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                  ccTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTCGCGACTGATGTtgctg  <  2:424357/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
GTTGTGCGGTGGTCAACCGTTCGGTATGCAGCGTGATTCGTTGCAACATGCGCCGCATATTATCGTGGCAACGCCGGGGCGTTTGCTGGATCACCTGCAAAAAGGCACGGTATCAC‑TGGATGCGTTGAATACGCTGGTGATGGATGAGGCCGACCGCATGCTGGATATGGGATTTAGCGATGCCATTGATGATGTCATCCGTTTTGCGCCTGCATCTCGACAGACGCTTCTGTTTTCGGCAACCTGGCCGGAAGCCATCGCTGCAATCAGCGGACGAGTGCAACGCGATCCTTTGGCGATTGAAATTGACTCAACAGATGCTTTGCCACCCATTGAACAACAATTTTATGAGACATCCAGCAAAGGCAAAATTCCTCTGTTGCAACGGTTATTAAGCTTGCATCAGCCATCCTCTTGCGTGGTGTTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTCGCGACTGATGTTGCTG  >  NC_000913/1409825‑1410414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.