Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,173,361	Δ2 bp	100%	pseudogene (1‑2/442 nt)	gatC ←	pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,173,361	0	C→.	100.0%	114.4	26	pseudogene (2/442 nt)	gatC	pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  new base (6/20):  ref base (0/0):  total (6/20)
*	NC_000913	2,173,362	0	C→.	100.0%	114.4	26	pseudogene (1/442 nt)	gatC	pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  new base (6/20):  ref base (0/0):  total (6/20)

GAACCGCCCTGATCCATTGAAGCCACCATACCCCCGG‑CTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATG  >  NC_000913/2173072‑2173620                                                                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                   aaaCGGCCCCGATCCATGGAAGCAACCATACCGCCGG‑CTTTTAACGCACCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTc                                                                                                                                                                                                                                                        <  2:26281/300‑1 (MQ=255)  aaCCGCCATGACGCATGTAAACCACCA‑ACCCCCGGTCTGGTAACGCCACAGCATTAGCGGCATGTTGATTGTGCAAACCAATAGTTTGCGTCTCTATCCACAGGGTGATGCGCATAAGGAGGACACACGAGATTAAGTTGCAGAACAGATGTACACGACGCACGGCGACAGCCATCGCCACGAATAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTTAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGt                                                                                                                                                                                                                                                         <  2:423352/299‑1 (MQ=255)      gCCCTGATCCATTGAAGCCACCATACCCCCGG‑CTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAACAAGAAATTAAAATGGTGAGTGGGATAAACATCAGGCGTGCCGACACCA‑‑CCGCCGTATGTCCcag                                                                                                                                                                                                                                                    >  1:263620/1‑300 (MQ=255)       cccTGAGCCATTGAAGCCACAATACCCCCGG‑CTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca                                                                                                                                                                                                                                                  <  2:256130/301‑1 (MQ=255)              acATGGAAGCCACCAACCCCCCGG‑CTTTTAACGCCCCAGAATTAGCCGCCAGTTGGGTGGGAAAACCAATAGTTGGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc                                                                                                                                                                                                                                           <  1:92511/300‑1 (MQ=255)                    aaGCCACCATACCCCCGG‑CTGTTAACGCCCCAGCATTAGCCGCCAGTGGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATc                                                                                                                                                                                                                                     <  1:272705/301‑1 (MQ=255)                         ccgagaCCCCCTG‑CCTTCAACGCCCCAGCATTAGTCGCCAGTTGGTTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGAGTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                                                <  2:6643/296‑1 (MQ=255)                          ccaTACCCCCGG‑CTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACCGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTCCCGATACCA‑‑CCGCCGTACGTCCCAGCAGCAACGCCGGATCACGGc                                                                                                                                                                                                                                >  1:321085/1‑300 (MQ=255)                              aCCCCCGT‑CTTTTAACGCCCCAGAATTAGCCGCCAGTTGGGTGCGCAAAACAAGAGTGTGGGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATTCACGGCGACCGCCATCTCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATc                                                                                                                                                                                                                           <  2:83088/301‑1 (MQ=255)                              aCCCCCGG‑CTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGCTCCACAGGGTGATGCTCATACTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGCAGCCGATGGTGGCAAGATCGCCAAACGCCAGCACCTGCTTACCCGGCACACACACAGCACTTAAAATGGGGAGTGGGATAAAAATCCGGCTTGCCGATACCA‑‑CCGCCGTCTGTCCCCGCACCCACGCCCGACCAAACCCaaa                                                                                                                                                                                                                            >  1:225765/1‑299 (MQ=255)                                        ttttAACGCCCCATGCTTAGCGGCCAGGTGGGTTTGAAAACAAATAGTGTGTGTCGCGATCCACAGGGTTATGCTCAAAATGAAGACCCCCGCGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTcc                                                                                                                                                                                                                  <  1:122236/301‑1 (MQ=255)                                        tgttAAAGCCCCAAGATTAGCCGCCAGTGGGGGTTGCAAACCAATAGCTGGCTGCGGGATCCACAGGGTGATGCTCAGAATGATGACACCCGAGATTAAGGGGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTcc                                                                                                                                                                                                                  <  1:430172/299‑1 (MQ=255)                                                                            cAAACACATAGTTTGCGTCGCGATCCACAGGGTGATGCTCCAAATGATGACACCTGAGATTAAGGTGCGGAACAGATTTCCACGACGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGcc                                                                                                                                                                              <  2:225770/301‑1 (MQ=255)                                                                            aaaaCCAAGAGGTTGCGTCGCGAACCACAGGGTGATGCTCATAATGACGACACCCGAGATTAAGGGGCTGAACAGAGTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGcc                                                                                                                                                                              <  1:249954/300‑1 (MQ=255)                                                                                                  ctCCACAGTGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTGCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATTGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                                                                                                                                        <  2:263625/300‑1 (MQ=255)                                                                                                  aTCCACAGGGGGATGCTCATAATGATGACACCCGAGATTAAGGTGGGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                                                                                                                                        <  2:53728/301‑1 (MQ=255)                                                                                                            tcatccTCATAATGATGGACCCCGCGGTTAAGGTCGGTAACCGATGTCCACGCTTCACGGCGACCGCCATCGCCACGAATAATCCGATGGTGGCAAGATCGCCAAACGTCAGCACCTGATTACCCGGCACACAAACAGCAATTACAATGGTTAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgat                                                                                                                                              <  2:221708/296‑1 (MQ=255)                                                                                                             cattcTCATAATGAAGACACCCGAGAGTAAGGTGAGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgatg                                                                                                                                             <  1:178569/297‑1 (MQ=255)                                                                                                                                cccGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGCTGGGCGTTCAACCATCCATTATGGGTTTAATCACCCGTGGc                                                                                                                           >  1:270292/1‑300 (MQ=255)                                                                                                                                                            caccaggCACGGCGACCGACATCGCCAAGACGACGCCGATGGTGGCACGACCGCCAAACGTCCGCACCTGATTACCATGCAAACAAACAGCAATTAAAAGTGGGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCATTCTGGAACTCCTGACCGCCGAACTTCGCCTCTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAc                                                                                              <  2:140972/295‑1 (MQ=255)                                                                                                                                                                           ccGCCATCGCAACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                                               <  2:270298/301‑1 (MQ=255)                                                                                                                                                                                                              tCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTCCCGCCCGCTGCAATCCACCTTTCACATCGTAACCCGCGAGGATGCCGATaa                                            >  1:54032/1‑301 (MQ=255)                                                                                                                                                                                                                          aacaCCTGACTACCCGGCACAAAAACAGTAATTAAAAGGGTGAGGGGGATAAAAAGCAGGCTTGCCGATACCA‑‑CCGCCCTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCGTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATca                                <  2:54032/299‑1 (MQ=255)                                                                                                                                                                                                                           acaCCTGATTACCCGTCAAACAAACAGCAATGAAAATGTTGAGTGGTATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTGTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac                               <  2:321093/300‑1 (MQ=255)                                                                                                                                                                                                                                  aatACCCGGCACACAAACAGCAATTAAAATGGGGAGAGGGATAAAAAGCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGTATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAcc                         <  2:136184/298‑1 (MQ=255)                                                                                                                                                                                                                                                        aTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGACCAAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTACTGAACGCCGAACGTCGCCTGTAAACGACTACGAGCCTGCGTAGCGATGGGCGTTAAAACAGCCATGATGGGTTTAAGCACCCGTGGCATTAGCAGACTCAATGCCGCCTCTTTTACAGCCCACTGCCATACACCTTTGACATGACAACCCTCGCGTATACCGAGAACAAGCCCACTCCCACACACCACAGAGACAGGCACGCAaacga  >  2:386569/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                   GAACCGCCCTGATCCATTGAAGCCACCATACCCCCGG‑CTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATG  >  NC_000913/2173072‑2173620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.