Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	381,545	0	A→C	100.0%	1.6	2	E65D (GAA→GAC)	insC1	IS2 repressor TnpA
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/0):  ref base (0/0):  total (2/0)

TTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATACCAGGAAGGAAGTCTTACTGCTGTCGCCGCCGGAGAACAGGTTGTTC  >  NC_000913/381256‑381555                                                                                                                                                                                                                                                                                                  |           ttACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGCTGATTGATGTCTTAGGGCCGGAGAAACGCAGGCGGCGTACCACACAGGAAAAGATCGCACTTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTCTTTCTCTGGCGTAAGCAATACCAGGAAGGAAGTATTCATACTGTACCCGCCGGAGACCAGGTTGTTc  >  2:309789/1‑300 (MQ=11) ttACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGCAGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCCGCCAGCCAGTTATTTCTCTGGCGTACGCAATACCAGGACGGAAGTCTTACTGCTGTCGCCGCCGGCGACCAGGTTGATc  >  2:344205/1‑300 (MQ=2)                                                                                                                                                                                                                                                                                                  |           TTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATACCAGGAAGGAAGTCTTACTGCTGTCGCCGCCGGAGAACAGGTTGTTC  >  NC_000913/381256‑381555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.