Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,620,8700T→G100.0% 0.1 5M560R (ATG→AGG) rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/3):  ref base (2/0):  total (2/3)
Fisher's exact test strand distribution p-value = 1.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

TCACCGACTGTTCCGGTTATGTAACCCGTTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCAC  >  NC_000913/3620816‑3621155
                                                      |                                                                                                                                                                                                                                                                                             
tCACCGACTGTTCCGGTTATGTAACCCGTTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAACCGGGACACCGTACGACGCGTGGGGAAAGGCCGTCCGTACCCCGCAGGGCGGGCCGACGCCCCGTATGGCATACggttgcgaccggc                                          >  1:72264/1‑288 (MQ=11)
tCACCGACTCGTCCGGTTAATGAACGCGTTATGACCTTGACCGCTGCGAGAAGAGGAAGGCGGGGCACCGGGAGAAAGGGCTGAGTCGGTACCGCGCATACGACAACCGTGGAGAGTGAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGAc                                          <  2:6076/300‑1 (MQ=11)
                            ttATGGACAGGACCGGTTCGGGCAGAGGGCGGCGGGGCACCGCGAGGAAGGGCTGAGTCAGTACCGGGCATACGACAGCCGTGGACAATTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACgg              <  1:183380/300‑1 (MQ=2)
                                gaccatgaccGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGCCCGCCGTCATTGCCCCGGACGGCAGCCGAAACAGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGGACCACGCAGGACGGGCAGACGGGGAAACTGGAATAAGATAATGCAGGCAGGATCACCCGACAGACCACTGAAAAACGCAGc          >  2:375672/1‑300 (MQ=11)
                                        acGCTGCGGGCAGAGGACGGAGGGGGACGGCGAGGAAGGGCGGAGTCAGGACCGCGCATATCACAGCCGTGGACAGTTAATTGCCGGGAAAGACACGCAGGTCCAGGACACGCGGTATGAAGACAACATGGCCGGGGACCTGACGGGCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCAcaccac  <  1:163286/299‑1 (MQ=11)
                                                      |                                                                                                                                                                                                                                                                                             
TCACCGACTGTTCCGGTTATGTAACCCGTTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCAC  >  NC_000913/3620816‑3621155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.