Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,334,502 C→A 10.1% R265S (CGC→AGC)  ispB → octaprenyl diphosphate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,334,5020C→A10.1% 4.2 0.0 ‑0.941R265S (CGC→AGC) ispBoctaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (17/20):  total (19/22)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.21e-01

GCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAAT  >  NC_000913/3334203‑3334797
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                       
gcgTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGATGCCGCTGCTGCATGCGATGCCACATGGCACACCAGAACAGGCACAGAAGATCCTGAACACAATCGAACAGGGTAACGGACg                                                                                                                                                                                                                                                                                                        >  1:314046/1‑301 (MQ=255)
   ttgtctatagtcagtccGCGCGTCTGTTTGAGGCGGCCGAGCAGTGGTCGGGGATTCTAGCTGGCTGTTCGCCGTAGGAGTAGAAAGGCCTGCAGGATTCTGGGCGCTATCTCGGCACTGCTTTCCAGTAGAGCGACGATTTACTCGATTACAAAGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACAAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCa                                                                                                                                                                                                                                                                                                     <  2:84270/286‑1 (MQ=255)
               aaaccgctgtgcggTTTGAGGCTGCCGTGCAGTGTTCCGGGATTCGGGCTGGCTGTACGCCTGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTAGCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAAcc                                                                                                                                                                                                                                                                                         <  2:356143/288‑1 (MQ=255)
                                     atgagcgtactgttcgtggctggcggctggagGGACGCCGGGGGAGGGGTAAGGAATGCAGGAATATGGGCGCTATCTCGGCACTGCTTTACAGTTGAACTAAGAGTGACTCTGTTACAAGGCCGATGGCGAACAGTTAGGTAACAATGTCGGCGAAGATCTGAACGAAGGTAAACCCACGCTGCCGCTGCTGCATGCTATGCATCAGGTCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACATGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGc                                                                                                                                                                                                                                                                    <  2:414715/276‑1 (MQ=255)
                                      gCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCtt                                                                                                                                                                                                                                                                  >  1:75145/1‑301 (MQ=255)
                                         gcgcAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCCTCTTCTGGACCCGGTTCTGGAAGCCATGAACGGCtgtg                                                                                                                                                                                                                                                               >  1:343501/1‑301 (MQ=255)
                                               tgtTCCGCGACTCGGGGTGTCTGTTCGCCGGATGAGTGTACATGCCTGCAGGATTATGGGCGCCAACTCGGCACTGCCTTACAGTTGACCGACGATTTACTCGATTAACACTCCTATGGCGAACAGTTAGGTAAAAAGGCCGGTGACCATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTTCCTGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACTGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGAt                                                                                                                                                                                                                                                            <  2:346200/298‑1 (MQ=255)
                                                      ggATTCTGGCTGTCTGTACGCAGGAGGAGGAGAAAGGCCTTCAGGATTATGGGCGCGATCTCGGAACTGCTGTCCAGTTGATCGCCGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAAt                                                                                                                                                                                                                                                   <  2:314054/300‑1 (MQ=255)
                                                                  gctgTACGCCGGAGGATGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTATGACACCGGTTCGGGAAGCACTGCACCCATGTGGATCCCTTGCATGGACGCCTccggg                                                                                                                                                                                                                                      >  1:394896/1‑297 (MQ=255)
                                                                                  ggagAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCTATTACAATGCCGATGGCGAACAGTGCGGTAAAAATGTCGGCGACGACCTGAACGAAGGTAAACCGACGCTGCCGCTGATGCATGCGAGGCCTCATGGCGCACGAGAACAGGCACAGATGATCCGTACGGGCCTCGCACGGGGTAAGGGTCGACATCTTCTGTAACCGGACCCGGCAGCAATGCAAGCTTGTGGAGCACTTGATTGGCCGGGTCAGCGTGCCAAGgagaacgca                                                                                                                                                                                                                      >  1:250322/1‑294 (MQ=255)
                                                                                                                                  ttggATCGACGGTGGACTGGATTAAAAGGCCGAGGGAGAACAGTTAGGTAAAAATGTCGGGAACGATCTGAACGAAGGTAAACCGACGGTGCCGGTGCTGCATACGATGCATCATGGCAGACCAGAACAGGAACAGATGATCCGTACCGCGAGCGAACAGGGTAACGGTCGCCAGCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGGGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACAccc                                                                                                                                                                              <  2:100197/290‑1 (MQ=255)
                                                                                                                                  gTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCCCTTGAATGGACGCGTCCGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGAAGCGTTACAGGTGCCCCCGGACAccc                                                                                                                                                                              >  1:100197/1‑293 (MQ=255)
                                                                                                                                     gATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCACCGAACAGCGTAACGGTCGCCCTCTTCTGGACCCGGTTCTTGAAGCAATGCACGCTTGTGGATCTCTTTATTGGCCGCTCCACCCTGCCGCGGCAGAACCCGACCACTCCCTCGCACCCTTCCCGGTGCTccccgccccaccttcgacagaa                                                                                                                                                                   >  1:221222/1‑282 (MQ=255)
                                                                                                                                             attgaagcgagtagacagcaaagtgCGACCAGTTAGGAAAAAATGTCGCCGAGGAGCTGAACGAAGGTACACCGCTGCTGCCGCTGCTGCATGCGATGCCTCATGGCACAACAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAAGGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTc                                                                                                                                                             <  2:61492/275‑1 (MQ=255)
                                                                                                                                              tttACTAGACTACAACGCCGAAGTCGAACAGTTAGGTAAAGATGTCGGCGAAGATCTGAACGAAGGGAAACCGCCGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAATAGGCACAGATGATCCGTACCGCCAGCGAACAGGGCAACGGTCGCCATCTTCTTGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGTCGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATc                                                                                                                                                          <  2:75145/301‑1 (MQ=255)
                                                                                                                                                  cTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGACCAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTTGAAGACATGAAAGCTTGCGGATCTCTTGAATGGACGCGTCAGCTTGCCGAGGCAGAAGCCATCCAAGCCATCGCATCGTTACCGGTGCCTCCGGACCCCCCTTgtctggaaccacccacggac                                                                                                                                                       >  1:15925/1‑281 (MQ=255)
                                                                                                                                                              gCCGATGGCAAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCCTGTGGATCTCTTGAATGGACGCTGCAGCGTGCCGAGGAAGAAGCAGACAACGCACTCGCAGCGTTACCGGTGCTCCCGGACACCCCGTGGCGAGGACCACTCATCGGCCTccccccccac                                                                                                                                           >  1:168513/1‑291 (MQ=255)
                                                                                                                                                                    ggCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGCATGGACGCGTCAGCGTGCCGGGGAAGAAGCAGACCACGCCATCGCAGCGTTACAGGTGCTCCCGGACCCCCCTTGGCGAGACGCACTCATCGGCCTCGCGCACATCGCTGtt                                                                                                                                     >  1:149335/1‑300 (MQ=255)
                                                                                                                                                                     gggAAAAGTTAGGTAAAAATGCGGGCGACGAACGGAACCAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCAGCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTAGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTc                                                                                                                                    <  1:332852/298‑1 (MQ=255)
                                                                                                                                                                          cAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACAGTAGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCCCCCACCGCTGTTCCAcgcg                                                                                                                              >  1:202462/1‑301 (MQ=255)
                                                                                                                                                                                                  gaTCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGCCACCCCTTGGCGAGACGCACTCATCGGCCTCGCGCCCATCGCTCTTCAACCCGATCGTTACTCCCCCCCCCCCATc                                                                                                       >  1:215427/1‑300 (MQ=255)
                                                                                                                                                                                                   aTCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTAGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTCCAGGTGCTCCAGGacac                                                                                                                                                                                >  1:179793/1‑226 (MQ=255)
                                                                                                                                                                                                   aTCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTAGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCAGGacac                                                                                                                                                                                <  2:179794/226‑1 (MQ=255)
                                                                                                                                                                                                                             cctccgaggcatgacaggaaggatGGCACACCAGAAAAGGCACAAATGGGCAGTACCGCCATCGAACAGGGTAGCGGTCGCCATTTTCTGAAACCGGTTCAGCAAGCAATGAACGCTTGTGGATCTTTGGAATGGACGCGTCAGCAGGGCGAGGAAGAAGCAGACAAAGCCATCTCAGCTTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGAACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTa                                                                           <  2:15925/279‑1 (MQ=255)
                                                                                                                                                                                                                              cgatgcagcAAGCGATGCATAAGGGGACACAAGAACAGGCACGGATGATCCGTAACGCCAGCGAACAGGGTAACAGGAGCCATCTTCTGGAAACGGTGCGGGGAGCAAGGAAGGATTGAGGGTCTCGTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCGCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTa                                                                           <  2:202464/297‑1 (MQ=255)
                                                                                                                                                                                                                                cgtcggcacgcgtagaaacatGCCACAACAAAACAGGCAAATCTGACCGGTACATCAATCGAGCAGGGTAAAGGTCGCCATCGTCTGGAACTGTTTCTGGAAGTAATGAACGCTTGTGGATCGCTTGAATGGACTCGGCAGCGTGCCGAGGAAGAAGCAGACAAAGCCAGCGCAGCGTTACAGGTTCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTTAACGCGATCGTTAATCCCCGCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTa                                                                           <  2:236287/280‑1 (MQ=255)
                                                                                                                                                                                                                                  gctgcATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTCCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCACCGCCCTCCCGCACATCGCTGTTCAACCCCACCGTTAATCCCCCCCCCCCACCCCCCCCACCCCGCGGGAGGGGTCCCGTAAGtt                                                                       >  1:372694/1‑300 (MQ=255)
                                                                                                                                                                                                                                   cggaaTGCGATGCAGAATGGCAACCACGAACAGGCACAGATGAGCCGTGCCGCCATCGAACAGGGTAACGGTCGCCCTCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGGGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTc                                                                      <  1:432712/296‑1 (MQ=255)
                                                                                                                                                                                                                                         gCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTGCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACACAGCAATCGCAGCGTTACAGGTGCTCCAGGACACCCCTTGGCGAGAAGCACTCATCGGACTGGCGCACACAGGTGGTCAACGGGATGTTTAATCGCCTCCCCCCATCCCGCCCATCCAGCGGGAGGGTTcacggcaagtccatcaaa                                                               >  2:77695/1‑284 (MQ=255)
                                                                                                                                                                                                                                                   atGGAACACAAGAACAGGCAAATGTGAACCGGCACGCCATCTAACTGGGTAACGTTCGCCAGCTGCTGGAACCGGTTCTGGCAGCAATGAACGCTTGTGGTTCTCTTGCATGGACGACTCAGCGTGCCGAGGCAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCTCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGGTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTc                                                     <  2:215431/301‑1 (MQ=255)
                                                                                                                                                                                                                                                     ggCACACCAGAACAGGCACAGAGGATCCGTACCGCCATCGAACAGGGGAACGGTCGCCATCGTCTGGAACCGGTTCGTGACGCAATGAACGCTTGTGGATCTCGTGAAGGTGCGCGTAAGCGTGACGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGTTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTc                                                     <  2:168514/299‑1 (MQ=255)
                                                                                                                                                                                                                                                         cacCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGCAGCAATGAACGCTTGTGGATCTCTTGAATGGCCGCGTCAGCGTGCTGAGGAAGAAGCAGACAAAGCCATCGCAGCGATACAGGTGCTCCGGGACACCCCTTGGCGAGAAGCACTCATCGCACTCGCGCCCATCTCTGTGCAACGCGATCGTTAATCCCCTCCCCTCATcc                                                                                                      >  2:406808/1‑246 (MQ=255)
                                                                                                                                                                                                                                                         cacCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACCAAGCCATCGCAGCGTTACAGGTGCTCCCGGACAGCCCTTGGCGAGAAGCACGCATCGGCCTCGCGCACATCGCTGTTCCACACGCTCGTTAATCCCCTCCCCTCATCACGCGCAGCCGACAGGACGGGTCCAGTACTGTCCCTCAACACACCTATTccactca                                               >  2:178845/1‑295 (MQ=255)
                                                                                                                                                                                                                                                           ccAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCAATCCCGCGCAGCGCGCGGGCTGGTTCCCGTACGTTCCATAAAAACACTTATTCAGCCCtat                                             >  2:183721/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                aaCAGGCACAGATGACCCGTACCGCCAGCGAACAGGGTAACGGTCGCCATCTTCGGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATaa                                           <  1:178844/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                aaCAGGCACAGAGGATCCGTACCGGCAGCGAACATGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATaa                                           <  2:343510/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gaacaGATGTGCCGTACGTCCATCAAACAGGGTAACGGTCGCCATCGTCTTGAACCGGTCCTGGAAGCAATGAACGATTGTGGATATCGTGAAGTGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAac                                          <  2:394905/293‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cagatacgccGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGCAGCAATCAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCCTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAac                                          <  2:19202/284‑1 (MQ=255)
                                                                                                                                                                                                                                                                        caGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCCTCGGCCTCGCGCACATAGCTGTTCAACGCGATCGTTAATGCCCCCCCCTCATCCCGCACAGCGCGCGGACTGGTTACAGTAAGCTCCATCAAAACACTTATTCCGctctctcac                                          >  1:19202/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                              aaaaGGGTAACGGTCGCCAGCTTCTGGAAACGGTTCTGGAAGAAATGAACGATTGTGGATCTCTGGAATGGACGCGGCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCTCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatat           <  2:149336/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                       aaggTCGCCAGCTTCTGGAACCGGGTGTGGAAGCAAGGAACGCTTGTGGAGCTCGTGAATGGACGCGGCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAat  <  2:221227/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                       
GCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGATTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAAT  >  NC_000913/3334203‑3334797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.