Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,836,077	A→G	6.0%	V450A (GTG→GCG)	hypF ←	carbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,836,077	0	A→G	6.0%	3.3 0.0 ‑0.1	67	V450A (GTG→GCG)	hypF	carbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (29/34):  total (31/36)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.61e-01

GTTTCGCCACGGCTGCTTCGCTGCCAAATCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTT  >  NC_000913/2835779‑2836372                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        ggtgcGCCACGGCTGCTTCGCTGCCAAACCGCCACCCGGAAGGGCCCCTGCAGGCAAGCCGCCCAGGTGCTCCCATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCCCAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGcac                                                                                                                                                                                                                                                                                                        <  1:1094882/296‑1 (MQ=255)          cGGCTGCGTCGCTGCCAAAGCGCCGCCCGGAAGCGCCACTGCAGGAAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCGGCCCCGCAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGc                                                                                                                                                                                                                                                                                              <  2:8145/301‑1 (MQ=255)           gggttgctCGCTGCACAAGCGCCACCCGGAAGGGCCACTGCAGGGAAGCCGCCCAGGGGCGCACATGCGCGATAGTTCACCCGAAGGACCTCGCCGCCCCACAAAGCGCCGTTCTCCGCCACACCGAGCCCGTCGAGCGTCAAAGCAATGACAGCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGc                                                                                                                                                                                                                                                                                              <  1:836199/293‑1 (MQ=255)                     cTGCCACATCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCACCAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAAt                                                                                                                                                                                                                                                                                    <  1:121044/300‑1 (MQ=255)                                    ccGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt                                                                                                                                                                                                                                                                    <  2:50256/301‑1 (MQ=255)                                               aCTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCCGGCGTAAc                                                                                                                                                                                                                                                          >  2:660067/1‑300 (MQ=255)                                                cTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGc                                                                                                                                                                                                                                                        <  2:456690/301‑1 (MQ=255)                                                           ccgccCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCCCGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGGCATGCACAACGTATTGCGGGGTAAAATCGTAGATgctgtcgcttatccacaactgacgcttccgg                                                                                                                                                                                                                                              >  1:1145384/1‑270 (MQ=255)                                                           ccgccCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATg                                                                                                                                                                                                                                                                            <  2:1145425/270‑1 (MQ=255)                                                             gccCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATCCTCTGCCAGACACGCCGCTGCATGGGCACGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACCGGCTGGAGAC‑ATAGCCCGGTAGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATg                                                                                                                                                                                                                                                                            >  2:505254/1‑268 (MQ=255)                                                                cAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGcct                                                                                                                                                                                                                                         >  1:368414/1‑298 (MQ=255)                                                                       ccacaagcgcgACAGTTCCGCCGGAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCCAACCGATACCGTCGAGCGTCAATGCAATTACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTATCGCTTCGCGCCACTGCATCTGGATGc                                                                                                                                                                                                                                 <  1:213013/294‑1 (MQ=255)                                                                                      tgtaaaagcgaccgcccgccgcCCCACAAAGCGCCGTTCTCCCCCATACCGTTACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAAt                                                                                                                                                                                                                  <  2:403156/285‑1 (MQ=255)                                                                                                 ccACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGc                                                                                                                                                                                                       <  2:1122433/300‑1 (MQ=255)                                                                                                    cTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCCGCCACTGATGCTCTGCCAGACACGCCGCTGCTTCGGCATGATGATGCAGTACCGTTTGCGTCGGCAGCTTCATTTCACGCGCCCACTTGCTGGCGAC‑ATAGCCCGGATGCGCGTCATGCAAAACGTATTGCGGGGTAAAATAGTAGATGTTTTGAATGAGGGGTAACGGTTCGCGACACTGCAACTGGATGCCATCGTCCCTGaact                                                                                                                                                                                                                  >  1:403149/1‑285 (MQ=255)                                                                                                             ccACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg                                                                                                                                                                                                                                                                                  >  2:191877/1‑214 (MQ=255)                                                                                                             ccACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg                                                                                                                                                                                                                                                                                  <  1:191875/214‑1 (MQ=255)                                                                                                                              cccccATACCGATACCGTCGAGCGTCTAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCCTCGTCACTTAAATCGCCCAGATGCTGACTCAACCCCGCTTGTTCAcccggcc                                                                                                                                                                           >  1:215446/1‑295 (MQ=255)                                                                                                                              cccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTAtttc                                                                                                                                                                                                                                                                                              <  1:434004/185‑3 (MQ=255)                                                                                                                              cccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTAtttc                                                                                                                                                                                                                                                                                              >  2:434011/1‑183 (MQ=255)                                                                                                                              cccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAAt                                                                                                                                                                                                                                                                                    >  2:41488/1‑195 (MQ=255)                                                                                                                              cccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACCCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCCCCccgcc                                                                                                                                                                           >  1:789850/1‑299 (MQ=255)                                                                                                                              cccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCCTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCCCTTAAATCGCCCAGATGCTGACCCACCACCCCTTGTTCACCGCCCa                                                                                                                                                                           >  1:641904/1‑300 (MQ=255)                                                                                                                              cccccATACCGAGACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAAt                                                                                                                                                                                                                                                                                    <  1:41488/195‑1 (MQ=255)                                                                                                                                    taccgataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt                                                                                                                                                                                                                                                                             >  2:194968/1‑196 (MQ=255)                                                                                                                                    taccgataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt                                                                                                                                                                                                                                                                             <  1:194966/196‑1 (MQ=255)                                                                                                                                                  aGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTc                                                                                                                                                       <  2:368421/300‑1 (MQ=255)                                                                                                                                                                       ccGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGAcacagca                                                                                                                                 >  2:215419/1‑301 (MQ=255)                                                                                                                                                                       ccGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTAACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCTAGaccccgc                                                                                                                                  >  1:258920/1‑295 (MQ=255)                                                                                                                                                                            aTCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGGTCCGCGCCGAGACCCAGCACAgg                                                                                                                             >  2:342804/1‑300 (MQ=255)                                                                                                                                                                             cccAGCGGCCACTGATGCCCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAAATCCGCGCCGAGACACAGCACAGGCg                                                                                                                           <  1:215418/300‑1 (MQ=255)                                                                                                                                                                                        cTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAacac                                                                                                                                                                                            <  2:374230/225‑1 (MQ=255)                                                                                                                                                                                        cTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAacac                                                                                                                                                                                            >  1:374223/1‑225 (MQ=255)                                                                                                                                                                                           aTGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCAGTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAg                                                                                                             <  2:56476/301‑1 (MQ=255)                                                                                                                                                                                                  gCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACCCCGCTTGTTCCCCGCGCCACAGGCAGAAGGTATTTTTCAGATCTGCGCCGACAcccagcccccgtcccttatacacctctgacgct                                                                                                       >  1:898168/1‑273 (MQ=255)                                                                                                                                                                                                  gCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCTGCGCCGAGAcacagcac                                                                                                                                <  2:898192/275‑1 (MQ=255)                                                                                                                                                                                                  gCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGATACACCGCACCGGCGGAACATTTTTaa                                                                                                               >  1:159827/1‑292 (MQ=255)                                                                                                                                                                                                  gCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTaa                                                                                                               <  2:159828/292‑1 (MQ=255)                                                                                                                                                                                                  gCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAACCGTAGATGTTTTGCATCAGTCGTAACGCTTCCCGCCCCTGCACCTGGATGCCATCGTCACTTAAATCGCCCAGATGCCGACCCAACATCCTTTGTTTCCCGCCCACCAGGCAGAAGGCATGTTCCAAATCCGGCCCGAAACCCAACATCGGCGGAACATTTTTAAACCCAGGAg                                                                                                      >  1:388252/1‑301 (MQ=255)                                                                                                                                                                                                        cacGCCGCTGCATGGGGAGTATGCGGCAGTACAGGTTGCGTCTGCCGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGGCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGGAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAg                                                                                                <  1:1014423/301‑1 (MQ=255)                                                                                                                                                                                                            ccgcTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGc                                                                                                                                                                                                                                                        >  1:749646/1‑145 (MQ=255)                                                                                                                                                                                                            ccgcTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGc                                                                                                                                                                                                                                                        <  2:749663/145‑1 (MQ=255)                                                                                                                                                                                                                      ggCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCgg                                                                                   >  2:212316/1‑300 (MQ=255)                                                                                                                                                                                                                            atgatgCAGTACCGTTTGGGGTGGAAGATTCATTTCACGCGCCCACTGGCTGGGGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAAGCAAAGCCAGCGCATCCGGCACATAc                                                                            <  1:212315/301‑1 (MQ=255)                                                                                                                                                                                                                              aatgaaaaaCCGGTTGCGTCGGCGCATGCATTTCACACGCCCACTGGGT‑GAGTCAACAGCCCAGTTGCGCGTCATACACTGCGTATTTGGGGGTGGAATCATAATTTTTTTGAATAAAGCGGATCGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTACATCGCCCAGATGCTGACTCAACGCCGCTTGTTCACCGCGCACCAGACAGAAAGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATAcc                                                                           <  1:601868/292‑1 (MQ=255)                                                                                                                                                                                                                               atgCAGTACCGTTTGCGTCGGCCGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCTCGTCATGCACACCGTATTGCGGGGTAAAATCGTCGCTGTTTTTCATCAGGCGTCACGCTTCGCGCCACTGCCTCTGGATGCCATCGTCACTTAAATCTCCCCGAAGCTGCCTCACCACCGCTTGTTCACCGCGCCCCACGCCGAAGGTATTTTTCAGACACGCGCCGAGACCCCGCCAAGGCGGAACATTTTTAAAGCCCGGAGGCAAACGCAgccaccccggacaataccc                                                                          >  1:1148975/1‑283 (MQ=255)                                                                                                                                                                                                                               atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAacac                                                                                                                                                                                            <  2:679129/186‑1 (MQ=255)                                                                                                                                                                                                                               atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAacac                                                                                                                                                                                            >  2:505329/1‑186 (MQ=255)                                                                                                                                                                                                                               atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAacac                                                                                                                                                                                            >  1:679117/1‑186 (MQ=255)                                                                                                                                                                                                                               atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGc                                                                                                                                                                                         >  1:403867/1‑189 (MQ=255)                                                                                                                                                                                                                               atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGc                                                                                                                                                                                         <  2:403874/189‑1 (MQ=255)                                                                                                                                                                                                                               atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATAcccc                                                                         <  2:789872/301‑1 (MQ=255)                                                                                                                                                                                                                               atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCCTCACTTAAATCGCCCAGATGCTGACTCAACCCCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGCACCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCTGAGGCAAAGCACACCCATCCGGCACATAccc                                                                          >  1:696548/1‑300 (MQ=255)                                                                                                                                                                                                                               atgCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGCATTGCGGGGTAAAATCGTAGATGTTTTGCATCCGGCGTAACGCTTCGCGCCACTGCATCTGGATGACATCGTCACTTAAATCGCACAGATGCTGACCCAACACCGCTTGTTCACCGCGCTCCAGGCAGAAGGTATTTTTCAGATCCGCGCCGCGACACAGCACAGGCGGAACTTTTTTAAAGTCCGGAGGCAACGCCAGAGCATACGGCAAAtctcc                                                                          >  2:601877/1‑296 (MQ=255)                                                                                                                                                                                                                               atgCAGTAACGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAacac                                                                                                                                                                                            <  1:505322/186‑1 (MQ=255)                                                                                                                                                                                                                                 gCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCGCAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATAccccc                                                                        >  1:1075675/1‑300 (MQ=255)                                                                                                                                                                                                                                    gTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGCGAcccagcac                                                                                                                                >  1:312605/1‑241 (MQ=255)                                                                                                                                                                                                                                    gTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGAcacagcac                                                                                                                                <  2:312608/241‑1 (MQ=255)                                                                                                                                                                                                                                     tACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGa                                                                    <  2:641915/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tggctggAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAat                              <  2:258923/301‑1 (MQ=255)                                                                                                                                                                                                                                                                           ggctggAGAC‑ATAGCCCGGATGCGCGTCATGCGCAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTcgc                                                   >  1:282599/1‑279 (MQ=255)                                                                                                                                                                                                                                                                           ggctggAGAC‑ATAGCCCGGATGCGCGTCATGCGCAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTcgc                                                   <  2:282602/279‑1 (MQ=255)                                                                                                                                                                                                                                                                           ggactgagaC‑ATAGCCGGTGAGGGCGTCAGGCACAACGTATGGCGGGGTAAAATCGGAGATGTCTTGCATCATGTGGAACGCGTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCACCACCGCGTGTTCACCGCGCAACAGGCAGAAGGTATTTTTCAGAGCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAat                              <  1:660056/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                               aTGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGAt           <  2:215447/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gcgcGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGt         <  2:696561/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     aagaTGCACAACGTATTGCGGGGTAAAACCGTAGATGTTTTGCATCAGGCGTAACGCGTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCgg    <  1:606900/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       cATGCGCAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGtt  <  2:1075713/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        aTGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCgg    >  2:606909/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        GTTTCGCCACGGCTGCTTCGCTGCCAAATCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGAC‑ATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTT  >  NC_000913/2835779‑2836372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.