Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	4,396,100	Δ3 bp	1.8%	coding (36‑38/1338 nt)	amiB →	N‑acetylmuramoyl‑l‑alanine amidase II

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4396103 =	166 (1.230)	3 (0.020)	3/532	NT	1.8%	coding (39/1338 nt)	amiB	N‑acetylmuramoyl‑l‑alanine amidase II
	?	NC_000913	= 4396099	163 (1.250)					coding (35/1338 nt)	amiB	N‑acetylmuramoyl‑l‑alanine amidase II

TCTGCCGCTTCACCGCTTCGGTTTTACCGTTTTCGGTAAGATCGACCACCAGCCGTAGCGTTTGTGCATCTTTAGGCGTTCCAGAGCGAATCGCCTTCACCAGATTATTGCCGCTGAACAACAACGGCAGTCCCTGAATCACGCCCGTTTGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4396407‑4396103 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagcGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATTTCGACATCCGGGTCAGGAAGAACACCTGTACCTTGTTGTGGCCACTCCACCAGGCAGATGGCATCGTTGGCAAAATAATCGCGGATCCCCATAAACTCCAGCTCCTCGGGATCGGCAAGGCGGTACAAATCAAAGTGATAGACCATTAAGT  <  NC_000913/4396099‑4395802                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             TCTGCCGCTTCACCGCTTCGGTTTTACCGTTTTCGGTAAGATCGACCACCAGCCGTAGCGTTTGTGCATCTTTAGGCGTTCCAGAGCGAATCGCCTTCACCAGATTATTGCCGCTGAACAACAACGGCAGTCCCTGAATCACGCCCGTTTGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAG                                                                                                                                                                                                                                                                                                                <  2:1012843/301‑1  CTGCCGCTTCACCGCTTCGGTTTTACCGTTTTCGGTAAGATCGACCACCAGCCGTAGCGTTTGTGCATCTTTAGGCGTTCCAGAGCGAATCGCCTTCACCAGATTATTGCCGCTGAACAACAACGGCAGTCCCTGAATCACGCCCGTTTGTTTGATATCGAACGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCCGAGAACGTCCCGGCACCCACCGGCGTGCCCAcccg                                                                                                                                                                                                                                                                                                                >  1:1154083/1‑296     CCGCTTCACCGCTTCGGTTTTACCGTTTTCGGTAAGATCGACCACCAGCCGTAGCGTTTGTGCATCTTTAGGCGTTCCAGAGCGAATCGCCTTCACCAGATTATTGCCGCTGAACAACAACGGCAGTCCCTGAATCACGCCCGTTTGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCAGC                                                                                                                                                                                                                                                                                                            <  2:356088/301‑1                                        GATCGACCACCAGCCGTAGCGTTTGTGCATCTTTAGGCGTTCCAGAGCGAATCGCCTTCACCAGATTATTGCCGCTGAACAACAACGGCAGTCCCTGAATCACGCCCGTTTGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCAT                                                                                                                                                                                                                                                                         >  2:654273/1‑301                                                                                                                                     CCTGAATCACGCCCGTTGGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGC                                                                                                                                                                            <  1:654256/301‑1                                                                                                                                                                                          TGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCAGCGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATTTCGACATCCGGGTCAGGAAGAACACCTGTACCTT                                                                                                                       >  2:675862/1‑301                                                                                                                                                                                                                                                                                                                GCGTCGCTACCACCCAATTTCTGAGCCGATACATCGTCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCGCTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATTTCGACATCCGGGTCAGGAAGAACACCTGTACCTTGTTGTGGCCACTCCACCAGGCAGATGGCATCGTTGGCAAAATAATCGCGGATCCCCATAAACTCCAGCTCCTCGGGATCGGCAAGGCGGTACAAATCAAAGTGATAGACCATTAAGT  <  1:1014295/300‑1                                                                                                                                                                                                                                                                                                                 CGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATTTCGACATCCGGGTCAGGAAGAACACCTGTACCTTGTTGTGGCCACTCCACCAGGCAGATGGCATCGTTGGCAAAATAATCGCGGATCCCCATAAACTCCAGCTCCTCGGGATCGGCAAGGCGGTACAAATCAAAGTGATAGACCATTAAGT  <  1:120043/299‑1                                                                                                                                                                                                                                                                                                                 CGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATTTCGACATCCGGGTCAGGAAGAACACCTGTACCTTGTTGTGGCCACTCCACCAGGCAGATGGCATCGTTGGCAAAATAATCGCGGATCCCCATAAACTCCAGCTCCTCGGGATCGGCAAGGCGGTACAAATCAAAGTGATAGACCATTAAGT  <  2:60043/299‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             TCTGCCGCTTCACCGCTTCGGTTTTACCGTTTTCGGTAAGATCGACCACCAGCCGTAGCGTTTGTGCATCTTTAGGCGTTCCAGAGCGAATCGCCTTCACCAGATTATTGCCGCTGAACAACAACGGCAGTCCCTGAATCACGCCCGTTTGTTTGATATCGAGCGCCACGGTGCGTTTGCTTTGATGGCTAAACGCATAATCAGGATCGCCAATAAAACTCAACGTTATCCGCGCCTGTTGATTACCGTTAGAAACCTGAATATCAGAGAGCGTCGCGGCACCCACCGGCGTGCACAGCAGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4396407‑4396103 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagcGTCGCTACCAACCAATTTCTGATGCGATACATCATCCCGCCACCTTTCAAAGGTTAACCGGCTAAACGCGCCAGCAACAATTCACCCGCAGAGGAAACCGCACTCACGCGCGCCTCACGGCCTTGTGCCTGATAATCAATGTGTATTTCGACATCCGGGTCAGGAAGAACACCTGTACCTTGTTGTGGCCACTCCACCAGGCAGATGGCATCGTTGGCAAAATAATCGCGGATCCCCATAAACTCCAGCTCCTCGGGATCGGCAAGGCGGTACAAATCAAAGTGATAGACCATTAAGT  <  NC_000913/4396099‑4395802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.