breseq  version 0.26.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
JC 521,734 Δ90 bp 4.8% coding (1319‑1408/2415 nt) ybbP → putative ABC transporter permease
RA 1,097,158 C→T 6.5% intergenic (+329/+407) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser
JC JC 1,293,124 IS5 (+) +4 bp 12.4% intergenic (‑202/‑400) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,356,662 C→T 6.8% S150N (AGC→AAC)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein
RA 1,466,579 G→C 6.5% pseudogene (1188/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,529,218 C→T 100% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,708,844 C→G 10.1% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,847 G→A 8.6% E643E (GAG→GAA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,854 C→G 7.8% Q646E (CAA→GAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,985 G→C 6.9% A689A (GCG→GCC rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,778,806 T→A 10.8% K166* (AAA→TAA)  ydiP ← putative DNA‑binding transcriptional regulator
RA 2,173,359 Δ1 bp 6.1% pseudogene (4/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,304,523 G→C 7.6% intergenic (+130/+585) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,455,411 A→G 5.4% L79P (CTG→CCG)  yfcV ← putative fimbrial‑like adhesin protein
RA 3,277,038 Δ1 bp 6.1% coding (37/336 nt) prlF → antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
RA 3,560,455 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,560,460 A→T 10.7% pseudogene (146/150 nt) glpR ← pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,809 Δ1 bp 55.0% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
JC 3,815,859 Δ82 bp 3.5% [rph][rph] [rph], [rph]
JC 3,815,909 Δ10 bp 5.2% pseudogene (632‑641/669 nt) rph ← ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 4,116,407 C→A 100% M272I (ATG→ATT glpK ← glycerol kinase
RA 4,117,005 T→A 7.4% D73V (GAT→GTT)  glpK ← glycerol kinase
RA 4,183,258 G→A 100% E672K (GAA→AAA)  rpoB → RNA polymerase, beta subunit
RA 4,296,155 T→C 10.4% intergenic (+361/+281) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→G 28.7% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 34.1% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 23.0% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 13.7% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 8.7% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,304 A→C 6.9% intergenic (+510/+132) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 582991 584639 1649 45 [41] [42] 43 [tfaX]–appY [tfaX], appY
* * ÷ NC_000913 1211041 1212738 1698 43 [42] [38] 44 [mcrA]–[ycgX] [mcrA], icdC, iraM, [ycgX]
* * ÷ NC_000913 1978503–1979269 1979692 424–1190 44 [0] [42] 44 insB1–insA insB1, insA

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1096961 =59 (0.550)15 (0.200) 7/380 NT 22.5% intergenic (+132/+604) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913 = 1097503 62 (0.820)intergenic (+674/+62) ycdU/serX putative inner membrane protein/tRNA‑Ser
* ? NC_000913 1207790 =42 (0.390)46 (0.450) 36/508 NT 54.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 36 (0.350)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780543 (0.400)65 (0.640) 59/508 NT 63.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 36 (0.350)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1746589 =95 (0.880)5 (0.050) 5/524 NT 5.2% noncoding (74/77 nt) valW tRNA‑Val
?NC_000913 1746610 = 91 (0.870)intergenic (+18/‑90) valW/ydhR tRNA‑Val/putative monooxygenase
* ? NC_000913 = 189696986 (0.810)4 (0.040) 3/532 NT 4.4% coding (38/1365 nt) sdaA L‑serine dehydratase 1
?NC_000913 2514273 = NA (NA)noncoding (1/1345 nt) IS186 repeat region
* ? NC_000913 = 19785020 (0.000)38 (0.360) 32/524 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 2736907 =116 (1.070)4 (0.040) 4/534 NT 3.4% intergenic (+24/‑247) bamD/raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
?NC_000913 4502090 = NA (NA)noncoding (1426/1426 nt) IS4 repeat region
* ? NC_000913 = 4612024105 (0.970)3 (0.030) 3/524 NT 2.8% intergenic (+23/‑104) osmY/ytjA salt‑inducible putative ABC transporter periplasmic binding protein/uncharacterized protein
?NC_000913 = 4612030 108 (1.030)intergenic (+29/‑98) osmY/ytjA salt‑inducible putative ABC transporter periplasmic binding protein/uncharacterized protein