Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009134,507,8370G→C100.0% 2.5 4L261V (CTT→GTT) insI1IS30 transposase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/0):  ref base (2/0):  total (4/0)

CCATTCCTCTGTCCCATGTCAGTGATTTTCTGAGTTCTGACGGTAAACTCAGGAATTTGTCGGTAAGAGCCTGATTTACTGAGACAGAATCTTTGCCCCTGAGTCTAAGGATGATCGTATAACGTGATTTTCGGTCTACAAGTGTGGCTATATGAGAGTTTTTTGTACCTGAGACTAAATCGCCCTCCCAATGCCCTAGAGAGCGTCTGTTATCGATATTTCGGGAACGTTCGTGAATTGGTGTTCCGTTCACTATGTTAATCGTACCTCTTTCGCCTTTGCGGGTATGACGCCTGCCATGGCGAAGGCTATGCGACCGTCGCAGATGCTGTATATTCAGGTGGTGTAGCGCTTCACGGCTACGAAAGTACAGCGTTTTATAAATTGTCTCAGGTGATATTCGCAGCGT  >  NC_000913/4507729‑4508137
                                                                                                            |                                                                                                                                                                                                                                                                                                            
ccATTCCTCTGTCCCATGTCAGTGATTTTCTGAGTTCTGACGGTAAACTCAGGAATTTGTCGGTAAGAGCCTGATTTACTGAGACAGAATCTTTGCCCCTGAGTCTAACGATGATCGTATAACGTGATTTTCGGTCTACAAGTGTGGCTATATGAGAGTTTTTTGTACCTGAGACTAAATCGCCCTCCCAATGCCCCAGAGTGCGTTTGTTATCGATATTTCGGGAACGTTCGCGACTTGGTGTTCGTTTCCCTATGTTCATCCTGTCTCttctacacatccccgagcccacgatacgtag                                                                                                              >  2:337898/1‑272 (MQ=11)
     ccTCTGTCCCATGTCAGTGATTTTCTGAGTTCTGACGGTAAACTCAGGAATTTGTCGGTAAGAGCCTGATGTACTGAGACAGAATATTTGGCCCTGAGTCTAACGATGAGCGTATGACGTGATTTTCGGTCTACAGGTGGGGCTATGTGGGAGTTTTTTGTACCTGAGCCTACATCGCCTTCCCAATGCCCAAGATACTGTGTATTATCGATATTTCGGGAACGTTCGTGAATTGGGTTTCGGTTGACGGGGTTAATCGGACCTTTTGTCCCTTTgttgtgattgctcttggattgcggca                                                                                                         >  1:918849/1‑276 (MQ=11)
                                               cTCAGGAATTTGTCGGTAAGCGCCTGATTTACTGAGACAGAATCTTTGTCCCTGAGTCTAAGGATGATCGTATAACGTGATTTTCGGTCTACAAGTGTGTCTGTATGAGCGTTTTTTGTACCTGCGACTAAATCGCCCTCACAATGCCCTAGAGAGCGTCTGTTATCGATATTCCGGGAACGTTCGTGAATTGATGTTCCGTTCACTATGTTAATCGTACCTCTTTCGCCTTTGCGGGTATGACGCCTGCCATTGCGAAGGCTATGCGCcctgtttcttataaccgtctccgatcccacga                                                               >  2:472420/1‑271 (MQ=1)
                                                                                                      gTCTAAGGATGATAGTATAACGCGATTTTCGCTCTACAAGTGTGGCTATATGAGAGTTTTTTGTAGCTGAGACTAAATCGCCCTCCCAATGCCCTAGAGAGCGTCTGTTATCGATATTTCGGGAACGTTCGTGAATTGGTGTTCCTTTCACTATGGTAATCGTACCTCTTTCGCCCTTGCGGGTGTGACGCCTTCCATGGCAACGGCTTTACGACCGTTCCAGACGACGTATATTCAGGGGATGTGGACGTTCACGGCTCCGCACGTACCGAGGTTTTTCAAttttgtccagtcattttc         >  1:1278419/1‑284 (MQ=1)
                                                                                                            ggATGATCGTATAACGTGATTTTCGGTCTACAAGTGTGGCTATATGAGAGTTTTTTGTACCTGAGACTAAATCGCCCTCCCAATGCCCTAGAGAGCGTCTGTTATCGATATTTCGGGAACGTTCGTGCATTGGTGTTCCGTTCACTATGTTAATCGTACCTCTTTCGCCTTTGCGGGTTTGACGCCTGCCATGGCGAAGTCCATGCGCCCGTCGCCGATGATGTCTATTCCGGTGGTGTGGCGGTTCGCGGCTACGAAAGTCCAGACtttttttattttttatcgtgtgttttgttcggtt  >  1:13728/1‑271 (MQ=1)
                                                                                                            |                                                                                                                                                                                                                                                                                                            
CCATTCCTCTGTCCCATGTCAGTGATTTTCTGAGTTCTGACGGTAAACTCAGGAATTTGTCGGTAAGAGCCTGATTTACTGAGACAGAATCTTTGCCCCTGAGTCTAAGGATGATCGTATAACGTGATTTTCGGTCTACAAGTGTGGCTATATGAGAGTTTTTTGTACCTGAGACTAAATCGCCCTCCCAATGCCCTAGAGAGCGTCTGTTATCGATATTTCGGGAACGTTCGTGAATTGGTGTTCCGTTCACTATGTTAATCGTACCTCTTTCGCCTTTGCGGGTATGACGCCTGCCATGGCGAAGGCTATGCGACCGTCGCAGATGCTGTATATTCAGGTGGTGTAGCGCTTCACGGCTACGAAAGTACAGCGTTTTATAAATTGTCTCAGGTGATATTCGCAGCGT  >  NC_000913/4507729‑4508137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.