Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	300,196	A→G	56.3%	F247L (TTC→CTC)	paoC ←	PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	300,196	0	A→G	56.3%	45.5 ‑0.8 ‑0.1	39	F247L (TTC→CTC)	paoC	PaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  new base (18/4):  ref base (12/5):  total (30/9)
Fisher's exact test strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.29e-01

GGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGAC‑GTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGG  >  NC_000913/299951‑300489                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       ggCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGCGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGGTCGGGCGGCGAGGGCCGCCCGCAGCGCATCGCTTCTCCGGAGCACCCTGCCGCCAAACCCCCCCCCGCTATACgtgcggatacacccaccattcca                                                                                                                                                                                                                                                  >  1:891644/1‑278 (MQ=255)                                                                                                      ggTCGGCACCGATACGCAAGTGGTGAAGGGTGGCGGGGGGGGGGGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCg                                                                                                                                             <  2:891659/300‑1 (MQ=255)                                                                                                                                   gatgtggataagagacaggtgTGATGGGGAATAGAGGGGCGGGGGAGCATCACGTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGctc                                                                                                                <  2:1219135/283‑1 (MQ=255)                                                                                                                                           gcggTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGCGATAATACGCAAATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGCTCGGGGCGGACCCAGTCCACCATCTGCTTTGAGGTCCAGGGGCTAAGCTTATTTCCTACCCCACCTGCCATCCATGCATGCGTCCCCATCCCCTTCTGCCTCTGCTCCggg                                                                                                       >  1:857549/1‑301 (MQ=255)                                                                                                                                            aagggacgggggtTGTTGGGAAGAGGGGGGCGGGGGAGCATCACTTTAACCGGACTTTTCACCGCTCGGGCGCGGAGGGCCGCCAGCAGCGAATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCgggg                                                                                                      <  1:207609/290‑1 (MQ=255)                                                                                                                                                  gtggtgTTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCCGATCGGTGCGGCCCCCTTCAATCATCTGATTTGAGGTCCAGCGGGTAAGCTTATTTCCCTCCCAGACGGCCCTCCAGCTATCCGGCGCCATCCCCATATGGCCCTGCTCCGCGGCCgggg                                                                                                >  1:556685/1‑298 (MQ=255)                                                                                                                                                     gtgtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGCGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGACCTTTCAGCGTTTTTGCCAGATCGGTGCGGCAc                                                                                                                                                                                                          >  1:401628/1‑192 (MQ=255)                                                                                                                                                     gtgtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGc                                                                                                                                                                                                                                                          >  2:53923/1‑144 (MQ=255)                                                                                                                                                     gtgtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGCACTTTCCGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCCGCGGGTAAGCTTATTTTCATCCCAGACGGCCATCGAGGCCTGCGGCCCCCTCGCCATCTGGctc                                                                                                                >  1:1219089/1‑282 (MQ=255)                                                                                                                                                     gtgtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCCGATCGGTACGGCACCAGTCACTCCTCTGATTTGAGGTCCAGGGGGTAAGCTTATTTCCATCCCAGACGGCCATCTCGGCATGCGGCTCCATCCCCGTATGTCTCTGGTCCgggtcagcgtacg                                                                                             >  1:653313/1‑291 (MQ=255)                                                                                                                                                     gtgtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGGCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTTGTCCGGGGTCGTgtagg                                                                                             >  2:879998/1‑301 (MQ=255)                                                                                                                                                     gtgtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTCAGCTTATTTCCATCCCAGAAGGCCATCGAGGCATGCGGATCCATCGCCCTCTGGCTCTGGTCCGGGGTctggct                                                                                               >  2:429568/1‑294 (MQ=255)                                                                                                                                                     gtgtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCCTCGCCATATGGCTCTGGTCCggggcggcctctt                                                                                             >  2:207614/1‑292 (MQ=255)                                                                                                                                                     gtgtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCCTCGCCATATGGCTCTGGTCCGGGGTCGTggagg                                                                                             >  2:264360/1‑301 (MQ=255)                                                                                                                                                     gtgtTATTGGGAAGAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCAc                                                                                                                                                                                                          <  2:401634/192‑1 (MQ=255)                                                                                                                                                       gtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCCAGCCCTCCGCCGATATACGGGGCGATAATACGCACATTCCCCACGGGAACTGTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCCATCCTCTGATTTGAGGTCCAGAGGGTAAGCTTCTTTCCATCCCAGACGGCCCTCGGGGCCGTCGGCCACATCACCATCTTGTCCCGGTCCGGGGGCgtggcggtg                                                                                           >  1:1379622/1‑295 (MQ=255)                                                                                                                                                       gtTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTAAATACACTGATTTGAGTTCCCGAGGGTAAGCTTATTTCAATCCCAACCGGCCCTCGAGGCATGCGGCCCCATCGCCATATGTCTCTGGTACGGGGCCggtttggtt                                                                                           >  2:402596/1‑293 (MQ=255)                                                                                                                                                          aTTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCAATCGCCAGATGGCTCTGGTCAGGGGTCGTGTAGGTAGCa                                                                                        >  2:691164/1‑301 (MQ=255)                                                                                                                                                               gAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGGGTCGGTAGCATCAAt                                                                                   >  2:987689/1‑301 (MQ=255)                                                                                                                                                                       ggtcGGGGGAGCATCACTTTAACCGGACGTTTCACGGCTCGGGCGGCGCGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCAcc                                                                            <  1:987672/297‑1 (MQ=255)                                                                                                                                                                        ggCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTTCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGCCGGCCATCGAGGCATGCGGCTACATCGCCATATGGCTCTGGTCCGGGGCCGTGTTGGTAGCATAAATCTTTACCg                                                                           >  2:737731/1‑300 (MQ=255)                                                                                                                                                                            ggggAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTACCATCCCAGACGGACATCGAGACATGAGGCTCCATCGCCATATGGATCGAGTACGGGGTCGTGTAGGTAGCAGCAATCTTCACAGCAGCg                                                                      >  2:235364/1‑301 (MQ=255)                                                                                                                                                                               gAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGCTTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATCTGGCTCTGGTCCGGGGTAGTGTAGGTGGCACCACTCTTCCCCGCAGCggcg                                                                   >  2:157916/1‑299 (MQ=255)                                                                                                                                                                                 gCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCAGCCGATATACGGGGAGATAATCCGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCAGCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGTCATGCGGCTCCATCTCCATATGGCTCTGATCCGTGGCCGTGTAGGTAGCATAAATCTTCACCGCCGCGGAGGt                                                                 >  2:1187182/1‑301 (MQ=255)                                                                                                                                                                                      cctttccgcGGCCGTTTCCCCCCTGCGTCGGGCAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCGTGCCGCCAAACCCTCCGCCGAGATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGGGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAg                                                            <  1:264355/293‑1 (MQ=255)                                                                                                                                                                                       cTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCCGACGGCCATCGAGGCATGCGGCTCCATCCCCCTATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTACCCGCAGCGGAGGTGAAAg                                                            >  2:412292/1‑300 (MQ=255)                                                                                                                                                                                       cTTTAACCGGACGGTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTgtaggta                                                                                           <  2:639476/269‑1 (MQ=255)                                                                                                                                                                                               ggACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCt                                                                                                                                                                     >  2:675375/1‑187 (MQ=255)                                                                                                                                                                                                    tttCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCCATCTTCACCGCCGCGGCGGTGCAAGCCCCGTCAAAGTc                                               >  2:1095117/1‑300 (MQ=255)                                                                                                                                                                                                      tCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAGCAGCTTGCCGCCCAACCCTCCGCCGATATACGGGGAGATAATCCGCACATTCTCCACGGGAACTTTCAGCGTTCTTGTCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGCGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCCCCTCACCCGCGCTGAACCCCCCGTCAAATTccc                                             >  1:1140853/1‑298 (MQ=255)                                                                                                                                                                                                                   ggcgAGGGCCGCCACGAGAGAATCGACTCTCAGGAACAGCGTGCCGCCAAACCCTCCGCCGATAGACGGGGAGATAATACGCACAGTCGCCACGGGAACTTTCAGCGTTGTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGAC‑GTTTTTGTc                               <  1:412286/300‑1 (MQ=255)                                                                                                                                                                                                                              ccAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCCTATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGGAGAGGAGGTGAAAGCCCCGTCAAAGTCACCGAC‑GTTTTTGTCGGGCGTGGCtt                    >  2:339292/1‑300 (MQ=255)                                                                                                                                                                                                                                  cagcGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGAC‑GGTTTTGTCGGGCGTGTCTTCcggg               >  2:278246/1‑300 (MQ=255)                                                                                                                                                                                                                                  acgcgcATCGCGTCTCAGGAACAGCTGGCAGCCAACACCTCCGCCGATAGACGGGGAGATAATACGCACAGTCATCACGGGAACTGTCAGCGTGTTGGCCAGAGCGGGGCGGCACCAGTCAGTCAGCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGAC‑GTTTTTGTCGGGCGTGTCTTCcgg                <  2:969365/298‑1 (MQ=255)                                                                                                                                                                                                                                       cATCGCTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGAC‑GTTTTTGTCGGGCGGGTCTTCcggcgg             >  2:216433/1‑298 (MQ=255)                                                                                                                                                                                                                                            cTTCTCAGGAGCCGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATCCGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCCGAGCGTAAGCTTATTTCCATCCCAGACGGCCATCGCGGCATGCGGCCCCATCGCCATCTGGCTCTGGTCCGGGGTCGTGTGGGTAGCATCAATCTTCACCTCAGCGGAGTTGACAGCCCCGTCCACGTCACCGCC‑TTTTTTGTCGGGCGTGTCTTCCGGCTGCttctttg     >  1:123110/1‑295 (MQ=255)                                                                                                                                                                                                                                            cTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGCGGCCTGCGGCCCCATCCCCCTATGGCCCTGGTCCGGGGTCGTGTAGGTAGCATCAATCCTTCCCCCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGCC‑GTTTTTGTGGGGCGGCTCTTCCgccggcgtcacg      >  2:428983/1‑294 (MQ=255)                                                                                                                                                                                                                                            cTTCTCAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGGTTTTGCCAGATCGGTGCGGCACCATTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCAATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGCGGCGTGTAGGTAGCATCACTCTTAACCGAGACGCGGGGGCAAGCCCCGTCAAAGTC‑CCGTCGGTTTTTTTCGGCCGGGTCTTCCGTCGGCTggtggg     >  1:581291/1‑296 (MQ=255)                                                                                                                                                                                                                                               ctcAGGAGCAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCCGATCGGTGCGGCACCCGTCAATCATCTGATTTGAGGTCCCGAGGGTAAGCTTATTTCAATCCCAGACGGCCCTCGCGGCATGCGGCCACATCGCCATATGGCTCAGTTCCGGGGTCGGGTAGGTACCATCACTCTTGCCCGCAGCGCAGGTACAACATCCGCCAACTGCACCGCA‑GTTTTTGCCGGGCGTGGCTTCcgcccgccgtgtgcggg  >  1:272782/1‑286 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       GGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCACTTTAACCGGACGTTTCACCGCTCGGGCGGCGAGGGCCGCCAGCAGCGCATCGCTTCTCAGGAACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGAC‑GTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGG  >  NC_000913/299951‑300489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.