| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 463,152 | C→T | 7.6% | A413V (GCC→GTC) | ppiD → | periplasmic folding chaperone, has an inactive PPIase domain |
| Read alignment evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | change | freq | score | reads | annotation | genes | product | ||
| * | NC_000913 | 463,152 | 0 | C→T | 7.6% | 19.6 ‑0.9 ‑0.0 | 171 | A413V (GCC→GTC) | ppiD | periplasmic folding chaperone, has an inactive PPIase domain |
| Reads supporting (aligned to +/- strand): new base (5/9): ref base (75/79): total (83/88) | ||||||||||
| Fisher's exact test strand distribution p-value = 4.11e-01 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.62e-01 | ||||||||||
Only 100 of 174 total aligned reads displayed.
GATATGGGTTGGTTAGAAGATGCCACTATCCCGGACGAACTGAAAAATGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAG > NC_000913/462854‑463448 | gATAGGGGTTGGTTAGAAGATGCCACTATCCCGGACGAACTGAAAAAGGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACAACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGcc < 2:595677/300‑1 (MQ=255) atatGGGTTGGTTAGAAGATGCCACTATCCCGGACGAACTGAAAAATGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCACCGACACCGAGTCTCTGGCCGGTGCAGAGCAGGCTGCCGCCGTTAACGCCa > 2:86793/1‑300 (MQ=255) ggttAGAAGATGCCACTATCCCGGACGAACTGAAAAATGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGACATCGTTAGACGAAGTACGTGACGACATTGCGGCGAACGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGAAGCAGAAAGTGAGCGATGCAGCAAGAAACGACACAGCGTCTCTGGCCGGTGCGGAGGAAGAGGACCGCGTTGAGGCCCCTCAGACggg > 1:100613/1‑301 (MQ=255) ggttAGAAGATGCCACTATCCCGGACGAACTGAAAAATGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAACGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCCAGCAACACCACCGAGTCTCTGGCCGGTGCCGAGCCAGCTGCCGGCGTTAAAGACACCCAGACggg > 1:1221041/1‑301 (MQ=255) aggCCACTATCCCGGACGAACGGAAAAATGCTGGGCTGAAAGAAAAAGGCCAACTGGCTGGTGTCATCAAATCTTCGGTCGGTTTCCGTATTGTACGGCTGGACGAAATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGCCATTGCTGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTc < 2:748895/297‑1 (MQ=255) ccACTATCCCGGACAAAATGAAAAATGCTGGTCTGAAAGAAAAATGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAACGAAAGTAAAATCGTTAGACGAAGTACGTGACAAAATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACGACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCaa < 2:664854/301‑1 (MQ=255) cccccGGACGAGCTGAAAAATGCTGCTCTGAAAGCAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGTTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGACAGTGAAATCGTTAGACTACGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAGAAGCCCCCACTGCGTACTCCGCGCTGCACCAGAATGCGAGGCCTGCGGCGAGCCACTGCACCGACTCCCTGGCCGTGGCAGACCAAGCTGCCCCCGTTAACGCCCCTcggcgcgcttccgtcgccatgctta > 1:811072/3‑277 (MQ=255) aTCCCGGACGAACTGAAAAATGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTATGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACCCGAAAAAGCCCTCGATCCGTACTACGCGCTGCAGCAGAACGTTAGCGATGCGGCAAGCAACGACACCGAGTCCCTCGCCGGTGCAGAGCAAGCTGCCGGCGTTACAGCCACCCAGACGGGGTGGTTCAGCAAAGC‑Ta > 2:610336/1‑301 (MQ=255) aTCCCGGACGAACTGAAAAATGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCCACGACACCGAGTCCCTGGCCGGTGCCGAGCAAGCTGCCGGCGTTAAAGCACCTCCGACGGGTGGGTCCGCGAAAGA‑Ta > 1:547778/1‑301 (MQ=255) atGAAAAATGCTTGTATGAATGAAAAAGGCAAACGGTTGGGGGGAACAAAAGCTGTGGCCGGGTTCATGATTTGAGGGTTGGACGACATCCAGCCAGCGAAAGTTAAATCGTTAGACGAAGTACTTGACGACATTGCGGCGAAAGTGAAACACGAAAAATCCCTCGATGCGTACTACGCGCGGCAGCAGAAAGGTAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAag < 1:1534754/300‑1 (MQ=255) gAAAAATGCTGGTCTGAAAGAACAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCAAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGAGCTGCAGCAGAACGTGAGCGCTACGGCAAGCACCGACGCCGAGTATCGGGCCGGTGCCGCGCCAGCTGACGTCGTTAAAGCCACTCAGACGGGTTGGTTGAG‑AAAGACTAGACTGCCGGaataa > 2:1062970/1‑298 (MQ=255) aTGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACGCCGACGGGTTGGTTCAGCAAGAA‑TACCCGCCCGGAAGAGTTGaa > 1:173063/1‑301 (MQ=255) ttGGTCGAAAAGAAAAATGCCAACTTCCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACtt < 2:682268/300‑1 (MQ=255) tgggTCTGAAAGAAAAAGGCCAAATGTCTGGTGTAAGCAAAGCTTCGGTAGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACTAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCGCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAAc < 2:1558729/297‑1 (MQ=255) ggCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGACCAAGCTGCCGGCGTTCAAGCCCATCACACGGGTTGGTTCAGCAAAGA‑TAACCTCCCGGACGAGTTGAACTCCAAGCCGGTTGCAGAc > 1:564546/1‑300 (MQ=255) cAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCACTCGATGCGTCCTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACCCGGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTCACGACAATCAGACGGGTTGGTTAAGCAAAGA‑GACACAGCCGGACGCGTTGAACTTCAAGCGGTTTGCCGACGGTa > 2:1511150/1‑301 (MQ=255) aCTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGGGAAACACGAAAAAGCCCTCGATGCTTACTAGGCGCTGAAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACGGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGGGTTAAAGCAACGACGACGGACTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTGGAACTTCAAGACGGTTGCCGGCTCTaa > 2:1164711/1‑299 (MQ=255) gtgtgggttaatcaAATCTTCGGTCGGTTGCCTGATTGGACGCCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAAcc < 1:872644/247‑1 (MQ=255) gTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCTGCGAAAGTGCACTCGTTCGACGAAGTACGTGACGACATTTCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGAGGCACGCAACGACACCGATTCTCTGGCCGGTGCAGAGCAAGCGGACGGCGGTAAAGCCACTCAGACCGGTTTGTTCAGCACACA‑TAACCTGCCGGAAGAGTTGCACGTATAGCCGGTGGcctcgcacactcgt > 1:564491/1‑289 (MQ=255) tGGTTTAATCAAATCTTCGGGAGGTGTTGTGATGGTACGGCTGGACGAAATTCAACCAGCGAAAGGGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAAc < 1:951480/301‑1 (MQ=255) ggTGGCAACAAATCTTAGGCGGGTTTGATGATTGTACGTCAGGACGACATTCAGCAAGCAAATGGGAAAGCGTTAGACGAAGTACGGGACGACATGGCGGCGAAAGTGAAACACGAAAAAGCCCTCGGGGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCGCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAAc < 2:1370338/300‑1 (MQ=255) gctgtAATCAAATCTCAGGTCGGTTTCCGGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGCGACATCCTTAGCCGAAGTACGGGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAAc < 2:715608/298‑1 (MQ=255) tgttagtgaaTCTTCGGGCGTTTTACTGATGTGACAGTTGGACGAAATTGAGCCCGAGATAGTGAAATCACTAAAAGAAGTATGGAACGACTGTGTCGCGAAAGTGAAACAAGAAAAAGCCCTCGATGCGTACTACGAGCTGCACCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAAcgg < 2:451147/292‑1 (MQ=255) tgtCATCAAATCTGCGGTCGGTTGCCTTATTGTACGGCTGGACGACAGTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACAGTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAAcgg < 2:550651/300‑1 (MQ=255) aacgtcgggaggtTTCCGGACTGTACGGCTGGACGACATTCAGGCAGCGAAAGGGAAATCGGTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGAACGACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCGAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGGTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTa < 1:191062/291‑1 (MQ=255) aTCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAACAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAACA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTCACTTTAACGGCGGTCTGGTa > 2:801324/1‑301 (MQ=255) tctgtggtcggtTTACACAATGTACGCGTGGACGACATTCAGTCAGCGAAAGGTAAAGCGTTCGCCGAAGCACGTAACGACATTGCTGCGAAAGTGAAACTAGAAATATCCCTCGATGCGTACTACGCGATGCAGCAGAAAGTGAGCGACTCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCACGCTGCCGGCGTTAAAGCCACTCAGACGGTTTGGTTCAGCAAAGA‑TAACCTGTCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTa < 2:564500/295‑1 (MQ=255) tCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGACGTACGTGACGACATTGCGGCGAAAGTGAAACACGAACAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCCAGCTGACGGCGTTAAAGCCCCTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGCAGAGTTGACCGTACAGCCGGTTGcccgccccacactaaacggcggcctgggag > 1:1021525/1‑273 (MQ=255) ttCGGTAGGTGTCCTGATTGGACGCCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACAGTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑Gt < 2:1390557/301‑1 (MQ=255) gtTTCCTGATTGTGCGTCTGGACGACATTCAGCCAGCGAAAGAGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCAATCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGAAACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGACGGCGTTAAAGCCACTCAGACGGGTGGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAAATTCAAGACGGTTGCCGACGCAATCTTTCAAGGCGGGCTGGGCG‑GGGAAAACgg > 2:692180/1‑301 (MQ=255) tttGCTGGTGGTACGGCTGAACGACATTCAGCCAGCGAAAGTGAAAGCGTTAGACGAAGTACGTGACGACATGGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACgg < 2:1264948/300‑1 (MQ=255) gATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAACGCCACTCAGCCGGGTTGGTTCAGCAAAGA‑TAACCTGCCGCACGCGTGCACCTTCACGCAGGTTTCCGACGCTCGCTCTACCGGCGCTCTgcgagggtgacagaggcgacgg > 1:687488/1‑280 (MQ=255) gATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGAAACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCCAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTCTATTTAACGGAGGTCTGGTTG‑TTGAAAACGGCGCGCCg > 2:875219/1‑301 (MQ=255) ggACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGCGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGTCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGGCACGCAGACGGGTTGGGTCCGCCAAGA‑TCACCTGCCGGACGACATGAACTTCCAGCCGGTTGCCGACGCTATCTTTAACCGCGCTCTGGTAT‑GTGAAAACGGCGCCCCggcacgcaccgc > 1:108160/1‑290 (MQ=255) aacATTCAGACAGAGAAAGTGAAATCGTTAGACGAATGACGTGACGACAGTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCGGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGGGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGAca < 2:176105/300‑1 (MQ=255) acATTCAGCCAGCGCAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCCGCAGAACGTGAGCGATGCGGCAAGCAACGACCCCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCCGCCGGGTTGGTTCAGCAAAGA‑TACCCTACCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTGCCTTTACCGGCGGTCGGGTAGTGTG‑CACCGGCGCACCGGGccgccacccttcca > 1:38081/1‑287 (MQ=255) acATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCTACGCTATCTTTAACGGCGGTCTGGTCG‑GTGACCACGCCGCGCCGGGCATCAACTCTgccc > 2:1028793/1‑297 (MQ=255) acATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATACGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCCGAGCAAGCTGCCGGCGTTAAAGTCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGCAGAGTTGCACTTCACGCCGGTTGCCGACGCTATATTTAACGGACGACTGGTGG‑GGGCAACCGGCGCGCAGGGCATCAACCCTGAcca > 2:450596/1‑299 (MQ=255) tCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACatcatc < 2:216778/301‑1 (MQ=255) ctccaacaaaaGTGAAATCGTTACACGAATTACGTGACGACATTGCGGCGAAAGTGAACCACGAAAAAGCCCTCGAGGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGTCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCAc < 2:1243036/293‑1 (MQ=255) cggacacgcaaaatggtgataagacacaggTAAGTGCCGACATTGATGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGGACTAGCGGATGAATCAGAAAGAAGGCGCTGCGGAAAGCAAAGACACCGAGTCGCTGGCCGGTGAAGAGCAAGCTGACGGCGTCAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTAGCTTTAACGGCGGCCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCAc < 1:924836/272‑1 (MQ=255) tccagcTAAATGGAAACCGTGTGACGCAGTACTGGACGACATTGCCGCACAAGGGAAACACGAAAACGCCCTCCATGCGTACTCCGCGCTGCAGCAGAAAGGGAGCTATGCGGCAAGCAACGACACCGTGGCTCTGGCCGGTGCAGAGCAAGCTTCCGGCGTTAAAGCCACTCAGACGTTTTGGTTCAGCAAAGC‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGTTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCAc < 1:545887/299‑1 (MQ=255) cGAAAGTGAAATCGTTAGACGAAGGACGTGACGACATTGCGGCGAAAGGGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAg < 2:1221087/300‑1 (MQ=255) aCGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCAGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTAAGCAAAGA‑TAACATGCCGGAAGAGATGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGAGGACGGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACAGCGCAttagg > 2:169521/1‑298 (MQ=255) aaGTACGGGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTAACATCATCACCGTAGACGGCGACCGCGCATTCGtgc < 2:547787/300‑1 (MQ=255) gTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCACCGACACCGCGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGC‑TAACCTGCCGGAAGCGTTGAAGTTCAAGCCGGTTGCCGACGCGATCTTTAAAGGCGGTCTGGTAG‑GTGAAAACAGAGCGCAGGGCATCAACTCTGACACCATCAcatgtatcttatacacatctccgcaccca > 2:924854/1‑272 (MQ=255) gTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTAAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGggctg > 2:1494724/1‑300 (MQ=255) gaCGGGACGAAATGGCGGGGAAAGGTAAAAACGAAAAAGCCCGCGAGGCGTACTACGCGTCGCAGCAGAAAGTGGGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGGGCAGAGCAAGCGGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGtgctg < 2:371857/298‑1 (MQ=255) cGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCCACGACACCGAGTCTCTGGCGGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTCG‑GTGAACACGGCGCGCCGTGCATACACTCTGACATCATCACTGTAGACGGGGCGCGCGCCTTCGTGCAgcgaa > 2:954767/1‑299 (MQ=255) cGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACCTCAGCACCGTAGCCGGCGACCGCGCATTCGTGCCGCGCa > 2:85908/1‑301 (MQ=255) aaagaccatggggcgAAAGGGAAAAACAAAAAAGCCCTGGGCGCGTAATACGCGCGGGGGGAGAAAGTGAGCGATGCGGCAAGCAAAGACACCGAGTCTCGGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATc < 1:1430831/289‑1 (MQ=255) acgaaAGTGCGGGGAAAGTGAAAAACGAAAAAGCCCTGGGTGCGTCCGACGTGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAg < 1:53203/301‑1 (MQ=255) acatgacggcgAAAGGGAAACACGAAAAAGCCATCGATGCGTACTACGCGCTGCAGCATAAAGTGAGCGAGGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAATGCCACGCAGACTGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTTAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCTTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGa < 2:38083/295‑1 (MQ=255) aaagtGCGTCGAAAGTGAAACACGAAAAAGGCCTCGATGGGTACTACGCGCAGCAGCAGAAAGCGAGCGACACCGCAAGCAACGACACCGGGGCTCTGGCCGGTTCAGAGCAAACTGCCGGCGTTAAAGTCACTCATACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGACCTTAAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCg < 2:764363/296‑1 (MQ=255) aaaaaGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAACGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGCAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAAc > 1:994564/1‑185 (MQ=255) aaacGCACTCGAAGCGGACTACGGGCTGCAGAAGAAAGTGGGCGAGGCGGCAAGCAGAGAAAGCGAATGTCGGGCCGGTGCAGAGCAAGCGGCCGGCGTTAAAGCCAATCAGACGGGTTGGTTCAGCAAAGA‑TAACCGGGCGGAGGAGTTGAACTTCAAGCCGGATGCCGACGGTATCTTTAAAGGCGGTCTGGTAG‑ATGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCg < 2:687499/301‑1 (MQ=255) aaaaGCCCTCGATGCGTACTACGCGGTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCg < 2:291769/301‑1 (MQ=255) cggcagcgtcgcaagggaaaaagggagaggcagAAAGGGGGAGAGGCGGGAAGAAACGACACCGAGTCTGTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGTCACTCAGACGGGTTGGGTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCg < 1:1537862/271‑1 (MQ=255) gATGCGTACTACGCGCTGCAGCAAAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCCGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCCGCAAAGA‑TAACCTTACGGCAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCGTTACCGTAGGTCTGGTAG‑GTGAAACCGGCGCGCCGGTCATACACTCTGACATCATCACCGTAGACGGCGACAGAGCGTTCGAGCGTCTCAGCAGCGCGCAAAACCCGGAAGCGGTGCAACCTTTGGAACATg > 2:1053350/1‑301 (MQ=255) aTGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGACGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACAGCGCAGTGGTGCCGCGCCTCCGCGGGCACAACCCGGACGCGGGGCAACCGTTGGCCGagtg > 1:693809/1‑298 (MQ=255) aTGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTACCGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGAAGACGACCGCGCCTTCGTGCTGCCCCTCATCGAGCCCAAACCGGAAGCGGCGAAACCGTGGGGAGATGt > 1:140972/1‑301 (MQ=255) gCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTCAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGACGAGTTGAACTTCAAGCCGGTTGCCGACGAGATCTTTAACGGCGGGATGGGAG‑TGGAGAACGACGCGCCGGTCATCAAATCTGACATCATCACCGTCGACGGCGACGGCGGATGCGCGCAGCGCATCAGCGCGCCCAAACGgggacgggtgcgaacgagggaccagcttt > 1:768115/1‑274 (MQ=255) gTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTACAGCCACTCAGACGGGTTGGTTCAGCAAAGC‑TACCCTGCCGGACGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCt > 2:119018/1‑164 (MQ=255) gTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGCCATCCTCACCGTGAACGGCCACCGCGCATTCGTGCTGCGCATCAGAGAGCACAAACCGGAAGCTGTGAAACCGTTGGCAGATGTTCAg > 2:1006838/1‑301 (MQ=255) gTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGtgc < 2:111597/248‑1 (MQ=255) gTACTACGCGCTGCAGCAGAAAGTGAGCGAGGCTGCAAGCAACGACACCGAGTCTCTGGCCGGTGTAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCt < 1:119014/164‑1 (MQ=255) cctacggggttgaggagAAAGGGGGAGGTGGGGCAAGCAAAGACACCGGGTGTCGGGCCGGGGCAGAGCAAGGTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGGAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGa < 1:1494667/286‑1 (MQ=255) ctacGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGATGGTTCAGCAAAGA‑TAACCTAGCGGAAGAGTTGAACTTCACGCAGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCAGCAACGCTGACATCAGCACCGTAGACGGCGACCGCGCATTCGTGCGGCGCATCAACGAGCACAAACCGGCAGCGGTGAAGCGGTTGGCCGGTGTTCAGGaa > 2:699544/1‑301 (MQ=255) ctacGCGCCGCAGCAGAAAGTGACGGGAGCGGCAAGCAACGACACCGATTCTCTGGCCCGGTCCTAGCAAGCTCCCGGCGGTAAAGCCCCTCAGACGGTTTGGTGCCGCAAAGA‑CACCCTGCCGGAATAGTTGAACTGCAAGCCGGTTGCCGACGCTATCTTTAACTGCGGGCTGGTAG‑GTGAAAACCGCGCGCCGGGCATCAACTCTGACATCATCACCTTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGaa < 2:421208/301‑1 (MQ=255) caagcggctgaagcaagaaggtaggaaTGCGGCAAGAAACAGCACGGAGGCGTTGGCCGGTGCAGAGCAAGCTGCCGGCGTGAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGGTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAgg < 1:801311/273‑1 (MQ=255) gctggggcagAAAGGGGGCGGGGGGGCAAGCAACGACAGCGAGTCGCTGGCCGGGGAAGAGTAAGCGGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGGAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGt < 1:866203/294‑1 (MQ=255) gcagcaGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATAACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGCGCCCACACCGGACACGGTGCACCCGTTTGCAGATGTTAATGAACAAGTTAAg > 2:630138/1‑301 (MQ=255) gcagcaGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATAATCACCGTAGACGGAGACCGCCCAGTCGGGCTGCGCAGCCACGACCACCACCCGGAAGCAGGGAAACCGAGGGCAGGGGTTAAggccaaaggaaaa > 1:138234/1‑290 (MQ=255) gcagAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTCCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGGCACTGTTCCGGAACAAGTTAAGCCa > 2:309157/1‑301 (MQ=255) ccgAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTATCTGGCCGGTGCAGAGCAAGATGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTCTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGc < 2:108163/297‑1 (MQ=255) cagAAAGTGAACGATGCTGAAAGCAAACACATCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCa < 2:1373442/300‑1 (MQ=255) cagAAAGGCAGGGAAGCGGCAAGGAACGACACCGCGGGGCTGGCCGGGTGAGAGCAATCTGCCGGCGTGAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGGTGCCGACGCTATCTTTAACGGCGGTCGGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGc < 1:13711/299‑1 (MQ=255) aaagtaGCGATGCGGCAAGCGACGACACTGCTTCGCGTGCTGGTGCAGATCAAGCTGCAGGCGTTAAAGTCATGCAGACGGGTTGGGTCAGCAAAGA‑TATCCTGCCGTAAGAGGCTAACTTCAATCCGGTGGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAATGGCGCGCCGTGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGt < 2:538297/296‑1 (MQ=255) tggagggcagCGGCAAGCAACTCCAGCGGCGCTCTGGCAGGCGCAGAGCAAGCTGCTGGCGGTAAAGCCACGCAGACGGGTTGGTTCAGCAAAGA‑TACCCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTc < 2:231975/292‑1 (MQ=255) gtggcgatggtgcaacgcaCGACACCGAGGCGCGGGCCGGTGCAGAGCAAGCGGCCGGCGTTAAAGCCACGCAGACGGGTTGGTTCAGCAAAGC‑GAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGCCGCGATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTc < 1:1168277/282‑1 (MQ=255) aagggggcgaagaaggacggggAGACTCGGGACGGGGCAGAGCGAGGGGCCGGAGGTAAAGCGACTGAGACGGGTTGGTTCGGGAAAGA‑TAACGTGCCGGAAAGGTTGGACAGCAAGCCGGTTGCGGAAGCTATCTTTAACGGCGGTATGGTAG‑GTGAAAACGGGGCGCCGGGCATCAACACTGACATCATCACCATAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGcac < 1:295526/279‑1 (MQ=255) ccgaacgcaacgtaccggtgcctgtGGCCGGTGCGTAGCAAGCGGGAGGACATAAAGCCGCGTAGACGGATTGTGTCGCGGAAGA‑GAACCTGCCGGAAGGTTCGACCGGAAAGCCGGTTGCCGACGCTATCGTTAACGGCGGTATGGTAG‑GTGAAAACGGCGCGACGGGCATCAACTCTGAAAACAGCAGCGTAGACGGCGACCGCGCATGCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGACCAAGTTAAGGCACTGGTTCAGAAcaacaa < 1:488600/277‑1 (MQ=255) gggaagcaaGAACACCGAGGAGCGGGCGGGTGAGGAGCAAGCGGCGGGCGTGAAGGCCACTAAGACGGGGTGGTGCAGCAAAGA‑TAACCGGCCGGAAGAGTTGAACGTCAAGCCGGTTGCCGACGCTAGCTTTAACGGCGGTCTGGTAG‑GGGAAAACGGCGCGCCGGGCATCAACTCTGACATCAACACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCAcaacaa < 1:630129/297‑1 (MQ=255) gaaaggaacagggggcgtGGACGGTGAAGAGCAAGGGGCCGGCGGTAAAGCCAATCAGACGGGTGTGTTCAGGAAAGA‑TAACCGGCAGGAAGAGTTGAAATTCAAGGCGGTGGCCGACGCTATCGTTAACGGCGGGCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTg < 1:1028749/282‑1 (MQ=255) gaaCGACCCCGAGTCTCTGGCCGGTGGAGAGCAGGGGTCGTGCGGTAAAGCCACTCAGACGGGTGGGGTGTGCAAAGA‑TAACCGGCCGGAAGCGTGGAATTCTAAGCCGGTGGCCGACGCTATGTTTAACGGCGGTCTGGTAG‑GTGAAAACTGCGCGCCGTGCATCAACTCTGACATCATCACCGGAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTg < 1:1276615/298‑1 (MQ=255) gACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGaaccaa > 2:1010235/1‑298 (MQ=255) acacCGAGTCTCCGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACGCAGACGGGTTGGTTCAGCAAAGA‑TAACCGGCCGGAAGAGGTGAACTTCAAGCCGGGTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAGCGGCGCGCCGGGCATCAACTCTGACATCAGCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGaacaac < 1:669445/300‑1 (MQ=255) gTCTCTGGCCGGTGCAGCGCAAGCTGCCGGCGTTAAAGTCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGAcatc < 1:455112/166‑1 (MQ=255) gTCTCTGGCCGGTGCAGCGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGCGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGCCTGGTAG‑GTGAAACCGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTTCGGCTCCGCGCGCACCAACCGTAAGCGGTAAAATCGTTGGCACATTGTCTGGAAAACTACATGGCATTGATTCAGCACCACAGAGccgcaccagaggagaca > 1:105143/1‑285 (MQ=255) gTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGTCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGCAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGAACAACAAAGCTGAACAACAGGCGaaa > 2:973907/1‑301 (MQ=255) gTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGTCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATAAACTCTGAcatc > 2:455120/1‑166 (MQ=255) gTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGGGCCCCAACCGGAAGCGGGACACCCGTTGGCAGATTTTCAGGCACACTGTAAGGCATTGGTTCAGcaccaagaagccaacacacagacaaga > 1:76087/1‑277 (MQ=255) gTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGaaa > 2:865646/1‑301 (MQ=255) tctcTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAACCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTAAAAACGGCGCGCCGGGCCTCAACTCTGACCTCATCACCGTAGCAGGAGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGCTGTTCAGGCACAAGTTAAGGCATCGGATCAGCACAACAAAGCTGaacacacggcggaag > 2:227254/1‑290 (MQ=255) ctctGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCGGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGt < 2:1195981/301‑1 (MQ=255) gTGCAGAGCAAGCTGCCGGCGTTAAAGTCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACGGCACATTCGTGCTGCGCATCAGCAAGCACAAACCGGAACCGGTGAAACCGTTGGCCTATGTTCAGGCACAAGTTAAGGCATTGGTTCAGCCCAACAAAGATGGACCACAGGCGACAGTGGCTACTgg > 2:1347725/1‑300 (MQ=255) gTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAAc > 2:917693/1‑243 (MQ=255) aaaaatccagtgggtAAAGTCCCTCAGAGGGGGTGGATCAGAAAAGA‑TAACCCACCGGAAGCGTTGAACTGAAAGCCGGTTGCAGACGCTATCTTTGACGGCGGTCTGGTAG‑GGGAAAACGGCGGGCCGGGCATCAACTCAGAAATCATCACCGTAGACGGCGACCGCGCAGTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAActgc < 1:973890/287‑1 (MQ=255) gCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCAAAAACAAGCTGCAAAACGGGCGAAAGGGGATGCTGAGAAAACGCTGGTTGGttt > 1:329719/1‑301 (MQ=255) aaGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCCCAACCAAGCTGAACAACAGGCGAAAGTGGCTGCTGAGCAAATGCGCGTGGAtttacaag > 2:748002/1‑295 (MQ=255) | GATATGGGTTGGTTAGAAGATGCCACTATCCCGGACGAACTGAAAAATGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGTGTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAAGTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGA‑TAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCTGGTAG‑GTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAG > NC_000913/462854‑463448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |