New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1828681	125 (0.820)	4 (0.030)	4/512	NT	3.1%	coding (1054/1479 nt)	astD	succinylglutamic semialdehyde dehydrogenase
	?	NC_000913	= 1828695	129 (0.870)					coding (1040/1479 nt)	astD	succinylglutamic semialdehyde dehydrogenase
Rejected: Frequency below/above cutoff threshold.

GCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1828373‑1828681 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccctgcttgCGCCGCGCTTATTACAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTTCGGACCGTTATTGCGCGTCTGGCGTTATGATACTTTCGATGAAGCGATTCGAATGGCGAATAACACTCGCTTCGGACTCTCTTGCGGTCTGGTTTCCCCCGAGCGGGAAAAGTTCGATCAACTGTTGCTGGAGGCGCGGGCGGGGATTGTTAACTGGAACAAACCG  <  NC_000913/1828695‑1828439                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        cggggggcaactggTACCGGAAGCACCAATTCCGCCCAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGCTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGAGCGAACTTTTCCCGCTCGGGGGAAACCCGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTC                                                                                                                                                                                                                                                                           <  2:213632/287‑1  cgGGGGCGATGGTTACCGGAAGCACCAAGGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAACCCCCGCCCGCGCCTCCAGCAACAGTTGAGCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCC                                                                                                                                                                                                                                                                          <  2:1168030/299‑1   tgggggtaaGGTTACAGGAAGTCACGACGCCGCAGGATGGTGCGGGAGTGGCAGCACCAGTAAGCGGTTTGTTCCAGTTAATAAGCCCCGCCCGCGCCGCCAGCAACAGTTGGGCGAACTTTTACCGCGCGAGGGAAACCAGACCGGAAGAGAGTCCGTAGCGAGTGTTATTCGCCAGTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTAAATGATCCCCGGCGTCAGCAACGATGTCa                                                                                                                                                                                                                                                                          <  1:1068646/291‑2     GGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCAGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCCACGCCTGTCCTTTCAATGATCCCCGGCGTCAGCAACGCTGTCCCT                                                                                                                                                                                                                                                                        >  2:93454/1‑300      ggcaagGGTTACCGGAAGCCACACGGCCGGCGAAGGGCGCGGTACTGTCACCCCCGGGAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGGGCCTCCAGCAACAGTTGATCGAACTTTGCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTG                                                                                                                                                                                                                                                                       <  1:232305/294‑1        gGATGGTGACCGGAAGCACCAATGCCGCCGAAGGGCGGCGGACGGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCGCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCT                                                                                                                                                                                                                                                                     <  1:230577/299‑1            GGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTAGCCATTCGAATCGCTTAATCGAAAGTATCATAACGCCAGACGCGCAATACCGGTCAGAACACCTCTTCACCTGGTCCGCCAGCAACGCCTGACCTTTCACTGACCCCAGGCGTAAGCAACGATTTCCCTGCcttcaa                                                                                                                                                                                                                                                                >  2:4251/1‑295                                               GTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCC                                                                                                                                                                                                                                                                          >  2:831638/1‑256                                                    GGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGCACACCTCTTCGTCTGGTACGCCGGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACCATGTCACTGCT                                                                                                                                                                                                                                                                     >  2:1527760/1‑256                                                          aagatacAGCGTTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCGGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGACATCGTTGCTGACGCCGGGGATCAT                                                                                                                                                                                                                  <  2:279129/294‑1                                                                                                                 tgacaGAACTTTGCCCGCCATGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTTCGGACCGTTATTG                                                                                                                                                           <  2:799426/296‑1                                                                                                                    gaGAACTTGTCCCGCTCGGGGGAAACCAGACCGAAAGATAGTCCGAAGGGAGGTGTATTCGCCATTCGAATCGCTTAAGCGAAATGATCATAACGCCAGACGCGCAAGAACGGTCCGAACACCTCGTCATCTGGTACGCCAGCAACGTCGGTCAGTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTTCGGACCGTTATTGCG                                                                                                                                                         <  2:171818/298‑1                                                                                                                                                                                                                                                                          aagagacCAATGGTCCCGGGCGTCAGCAAGGCTGTCCCGGCTTTCGCCGCGCTTATTAAAAGCGGGGACATCGGTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTTCGGACCGTTATTGCGCGTCTGGCGTTATGATACTTTCGATGAAGCGATTCGAATGGCGAATAACACTCGCTTCGGACTCTCTTGCGGTCTGGTTTCCCCCGAGCGGGAAAAGTTCGATCAACTGTTGCTGGAGGCGCGGGCGGGGATTGTTAACTGGAACAAACCG  <  1:279122/294‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        GCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1828373‑1828681 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccctgcttgCGCCGCGCTTATTACAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTTCGGACCGTTATTGCGCGTCTGGCGTTATGATACTTTCGATGAAGCGATTCGAATGGCGAATAACACTCGCTTCGGACTCTCTTGCGGTCTGGTTTCCCCCGAGCGGGAAAAGTTCGATCAACTGTTGCTGGAGGCGCGGGCGGGGATTGTTAACTGGAACAAACCG  <  NC_000913/1828695‑1828439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.