Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	1,326,599	Δ36 bp	9.1%	coding (1854‑1889/1896 nt)	yciQ →	enhancer of membrane protein expression; putative inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1326598	32 (0.210)	3 (0.020)	3/512	NT	9.1%	coding (1853/1896 nt)	yciQ	enhancer of membrane protein expression; putative inner membrane protein
	?	NC_000913	1326635 =	29 (0.200)					coding (1890/1896 nt)	yciQ	enhancer of membrane protein expression; putative inner membrane protein

CTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGCCTGAAGAGTTCAAACAGACTTTACTATCAGTGAGTCAGATGTAAGTTCTGACTCACTGTTGCACAAAAGCATATCAGACGCCTGGGAAGGCGACACCAAAAGGAAGCTCTATGAGCGAAAAGCTACAGAAAGTGCTGGCGCGTGCCGGCCACGGTTCTCGCCGTGAAATCGAATCTATTATTGAAGC  >  NC_000913/1326341‑1326928                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      cTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTAACGACGCACCTAAGTTGCGGGGCCAATACCAATTTGTTGGGGCGAAGTTATTCAGGTGGTGGTGCTGGCGGCGGGGCGGGTGGCGGCGGAGGTGGGGGCTGGtca                                                                                                                                                                                                                                                                                                 >  2:1146054/1‑299 (MQ=255)            tgaaaGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAACACGGAAACGGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATg                                                                                                                                                                                                                                                                                      <  2:1030353/299‑1 (MQ=255)                  tgggcgcaacgcacctgggACGGCGGCGGAAATGGGGTACATCGCAATGTGATTACGCGTTCGTAGGTTGGTCCATGTCGGCAAATGTGGGGACGGAATGGGCGTTACGCTTTGTCCCTTCGGTTTCTTCGGCGAGTCGCGCTCTAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATc                                                                                                                                                                                                                                                                                <  1:84182/284‑1 (MQ=255)                     cagcaACACACAGTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTCACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCTGAGGCGGGGGTGGCTGGTAATTCAGc                                                                                                                                                                                                                                                                                           >  1:928551/1‑287 (MQ=255)                     cagcaACACACAGTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGc                                                                                                                                                                                                                                                                                           <  2:928564/287‑1 (MQ=255)                               caGTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGAGCTCCAGAGATTGGCCGTACCGGCGTTTTATTCTCATTCCAGCCGCACCTAAGTTGCGGGGCCACTACCAGTTTGTTGGGGCGAAGTTTTTCCGGGGGGGTGGCTGGCGGCGGCCCGGTTGGGGGGGGGGGGGATGGCTGGTATTTAGACTGAGGTTTGTCGAAACAAGCtttt                                                                                                                                                                                                                                                                   >  1:1448814/1‑300 (MQ=255)                                 gTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCCTTACAGACGCACCTAAGTTGCGGGGCCAATACCCGTTTGTTTGGGCGAGGTTATTCCGGTGGTGGTGCGGGCGGCGCCGCGGGTGGCGGGGGGGGGGGTGGCTTGTAGTTACGCTGTTGGTCATGGAACCCAGCATTaa                                                                                                                                                                                                                                                                  >  1:1505148/1‑300 (MQ=255)                                   ggACGTGGGCGGAAAGGGGAAACACGGAAACTGGATGACTGGTTGCTAGGTTGGGCGATATCGGCAAATTTGGGGATGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAAcc                                                                                                                                                                                                                                                                <  2:404476/300‑1 (MQ=255)                                               ataGGGGAAACAGGGATATTGGATGATGCGGTGCTAGGTTGGGCTAGGCTGGCAAAGTGGGCGAGGGGATGGGCGTTACGCGTTGCCACGTCGCTTTATTCGGCGATTCGCGCTCCAGAGATTGCCCGGAACGGCGTTTGATTCTCAGTACAGACGCACCTGCGGTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAgtgt                                                                                                                                                                                                                                                   <  1:514492/299‑1 (MQ=255)                                                gtaaagaggcaacaacaaagcgtgggcgaaggcggaaagaggaaaaacggaaactggattacgagttgctaggttgggctatatcggcaaatttggggagggaatgggcggtacgcattgccccttcgctttcttcggcgattcgcgctccagagattgcccgtcacggcgttttattctcattacagacgcacctaagttgcggggccaataccagtttgttggggcgaagttattccggtggtggCTGGTAATTAAGCTTATGTTAATCGAAACAAGCTTTAACCATAAAGATAgtgt                                                                                                                                                                                                                                                   <  2:328421‑M2/53‑1 (MQ=255)                                                     gAAACCAGGAAACGGGAGTTCGCGTTGGTATGTTGGGCTATCGCGGTAAAGTTGGGGAGGGAATTGGCGTTCCGCATTGCCCCTTCGCGTTCTTCGGCGATTCGCGCACCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGAGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGGAGTAt                                                                                                                                                                                                                                              <  1:793854/300‑1 (MQ=255)                                                     gAAACAAGGAAACGGGATTACTGGTTGATATGTGTGGCTAGATCGGCAAATTTGGGGAGGGAATGGTGGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACTGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTAt                                                                                                                                                                                                                                              <  1:370121/300‑1 (MQ=255)                                                                             ttGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGTTGCCCATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTGCTTAAGCTGATGTTAATCTAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATa                                                                                                                                                                                                                     >  2:269977/1‑301 (MQ=255)                                                                                   ggTTGGGCTATATCGGCAAATTTGGGGAGGTAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGCTAGTGTAGTATGTTGCGCCTCAAATCCAGGCCGATCAAAGt                                                                                                                                                                                                                >  2:1172342/1‑300 (MQ=255)                                                                                                    ctaTTCGGGGGGGGAAGGGGCGGTACGCCTTGCCCGTGCGTGTGCTTCGGCGATTCGCGCTCCGGAGATTGCCCGTAGCGGCGTTTTATTCTCAGTACAGACGCACCTAAGTTGCGTGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCTGGCCGATAAACGTCGAGTCGTTTACTTAAgg                                                                                                                                                                                              <  2:231859/299‑1 (MQ=255)                                                                                                      ttttGGGAGGGGAATGGGCGTTACGCATTGGCCCTGCGCTTGCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGGTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGcc                                                                                                                                                                                            <  1:269970/300‑1 (MQ=255)                                                                                                                                                cttcGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGAt                                                                                                                                                                                                                                                        <  1:538560/199‑1 (MQ=255)                                                                                                                                                cttcGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGAt                                                                                                                                                                                                                                                        >  2:538568/1‑199 (MQ=255)                                                                                                                                                      cGATTCGCGGTCCAGAGATTGCCCGGAACGGGGTGTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGCCTGAAGAGTTCAAACAGACTTTACTATCAGTGAGTCAGATGTAAGTTc                                                                                                                                             <  1:1172309/300‑1 (MQ=255)                                                                                                                                                          tcgggcgcctggaatgtcccGTAACGGCTGTTTAGTCTCATGAAGGACGCACCTAAGTGGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGAGAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGCCTGAAGAGTTCAAACAGACTTTACTATCAGTGAGTCAGATGTAAGTTCTGACt                                                                                                                                        <  1:139487/284‑1 (MQ=255)                                                                                                                                                               cTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGt                                                                                                                                                                                                           >  2:810482/1‑229 (MQ=255)                                                                                                                                                               cTCCAGAGATTGCCCGTAAAGGCGTTTTATTCTAATTACAGACGCACCTAAGTTGCTGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACACGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGt                                                                                                                                                                                                           <  1:810471/229‑1 (MQ=255)                                                                                                                                                                     tgaTTTCCAGGAACGGCGTGTTATTCTCAGTACAGGCGTACCTAATGTGCGGTGTCAAGACCAGTTTGGTGGGGCGAAGTTATTCCGTTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGTGGTGGGGGCTGGTAATTAAGCTGATGTTAACCGAAACAAGCGTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGCCTGAAGAGTTCAAACAGACTTTACTATCAGTGAGTCAGATGTAAGTTCTGACTCACTGTTGcac                                                                                                                             <  1:1146022/300‑1 (MQ=255)                                                                                                                                                                          gaccGTAACGGCGTTGGAGTCTCATTACAGACGCACCTAAGTTGCGGGGGCAATACCAGTTGGGTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCGTTAACCATAAAGATAGTGTAGTATGTTg                                                                                                                                                                                                                                          <  2:1152363/185‑1 (MQ=255)                                                                                                                                                                          gCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCGGTTTGTTGGGGCGAAGTTATTCCGGTGGGGGTGCTAGCGGCGGCGCGGGTGGCGGAGGCGGTTGTGGCTGGTACTTGAGCTGCTGTTAATCGAACCAGGCTTTAACCATAAAGATAGTGTAGTATGttt                                                                                                                                                                                                                                          >  1:1152330/1‑186 (MQ=255)                                                                                                                                                                           cccGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGCCTGAAGAGTTCAAACAGACTTTACt                                                                                                                                                                    <  2:259940/256‑1 (MQ=255)                                                                                                                                                                                            aaagaatgaCAGACTCACCTAAGTTGCGGGGCCAAGAACAGTGTGGTGGGGCGAAGTTATGCCGGTGGGGGTGCGGGCGGCGGCGCAGGTGGCGGAGGCGGTGGTGGCGGGTAATTAAGCTGATGTTAATCGAAACAAGCTGTAACCATAAAGATAGTGTAGTATGTTGCGCCGCAAAGCCAGGCCGATAAACGCCGAGTCGTTTATTTAAGGCCTGAAGAGTTCAAACAGACTGTACTATCAGTGAGTCAGATGTAAGTTCTTACTCACTGTTGCACAAAAGCATAGCAGACGCCTGGGa                                                                                                      <  2:372780/293‑1 (MQ=255)                                                                                                                                                                                                cATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCATGCCGATAACCGTCGAGGCGTTTACTTAAGGCCTGAAGAGTTCAAACAGACTTTTATAACAATGACTCAGATGTAAGTTCTGACTCACTGATGCACCACAACATAACAGACgaattgag                                                                                                      >  1:372774/1‑290 (MQ=255)                                                                                                                                                                                                                          gttttattctcattacagacgcacctaagttgcggggccaataccagtttgttggggcgaagttattccggtggtggCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGAt                                                                                                                                                                                                                                                        <  2:1019529‑M2/48‑1 (MQ=255)                                                                                                                                                                                                                          gttttattctcattacagacgcacctaagttgcggggccaataccagtttgttggggcgaagttattccggtggtggCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGAt                                                                                                                                                                                                                                                        >  1:1019501‑M2/78‑125 (MQ=255)                                                                                                                                                                                                                            cAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGCCTGAag                                                                                                                                                                                       >  1:943770/1‑188 (MQ=255)                                                                                                                                                                                                                            cAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGCCTGAag                                                                                                                                                                                       <  2:943783/188‑1 (MQ=255)                                                                                                                                                                                                                                        gttgGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGCCTGGAGAGTTCAAACCACCTTTACGAACAGTGACACCGATGTACGTTCTGAATCACTGTGGCACAAAAGCACATCAGACGCGTGGGAAGGCGACACCACAAGGAAGATCAATAAGCCACAAGAGGCAGaaa                                                          >  1:1229115/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                            ataggtgttgtCTGCAAATTAAGCTGATGTAAATCGAAACAAGCTTTAACCATAAATATAGCTTAGTATGTTGCGCCTCAAAGCTAGGCCGATAAACGTCGAGTCCTTTACGTAAGGCCTGAAGAGTTCAAACAGACTTTACTATCAGTGAGTCAGATGTAAGTTCTGACTCACTTTTGCACAAAAGCATATCAGACGCCTGGGACGGCGACACCAAAAGGAAGCTCTATGAGCGAAAAGCTACATAAAGTGCTGGCGCGTGCCGGCCACGGTTCTCGCCGTGAAATCGAATCTATTATTg      <  2:507155‑M2/290‑1 (MQ=255)                                                                                                                                                                                                                                                                                                gtggtggCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGATTACTTAAGGGCTGAAGAGTTCAAACAGACTTTACTATCAGTGAGTCAGATGTAAGTTCTGACTCACTGTTGCACCAAAGCATATCAGACGCCTGGGAAGGCGACACCAACACGAAGCTCTATGCGCTAAAACCTACAGAAAGTGCTGTCTCGTGCCGGGCACGGTTCTCCCCTGGAACTCGAAACTCTtagtgcttg  >  1:507147‑M2/8‑297 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      CTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAAGGGGAAACACGGAAACTGGATTACGCGTTGCTAGGTTGGGCTATATCGGCAAATTTGGGGAGGGAATGGGCGTTACGCATTGCCCCTTCGCTTTCTTCGGCGATTCGCGCTCCAGAGATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTATGTTGCGCCTCAAAGCCAGGCCGATAAACGTCGAGTCGTTTACTTAAGGCCTGAAGAGTTCAAACAGACTTTACTATCAGTGAGTCAGATGTAAGTTCTGACTCACTGTTGCACAAAAGCATATCAGACGCCTGGGAAGGCGACACCAAAAGGAAGCTCTATGAGCGAAAAGCTACAGAAAGTGCTGGCGCGTGCCGGCCACGGTTCTCGCCGTGAAATCGAATCTATTATTGAAGC  >  NC_000913/1326341‑1326928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.