| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,427,383 | A→C | 100% | noncoding (1380/1542 nt) | rrsD ← | 16S ribosomal RNA of rrnD operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,427,383 | 0 | A | C | 27.5% | ‑6.7 / 2.5 | 4 | noncoding (1380/1542 nt) | rrsD | 16S ribosomal RNA of rrnD operon |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (2/0); total (2/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01 | |||||||||||
| Rejected as polymorphism: Not supported by required number of reads on both strands. | |||||||||||
ACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGC > NC_000913/3427092‑3427447 | acgtGAACCGCCGACCTCACCCTTATCAGTGCTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCGTCTGTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCAc < 2:82303/298‑1 (MQ=0) tttgacacaccGACCTCAGCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGGAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCAcc < 1:834445/294‑1 (MQ=1) aGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGACTCACAACGTGGTACGCGCCCTCCCGAAGGTTAAGCTCCCTACTTCTTTTCCACCCAACTCCCATGTTTTCACGGCCGGTGTTTCCAAGGCCCGGGACCTTCTCCCCCGTGGCTTTCTGACCCCCGTTTCCtcacttttcctcattctgggagtca > 1:1485332/1‑277 (MQ=1) aGCCTGTCGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGCTCCCACCGCAGGTTCCCCTACCTTTCCCTTCTTCCGACTTCACCCCAGTCATGCCTACCAAAGTGGTAAGCGCACTACCAACGGTTCAGCTACCTACTTCTTTTGCAACCCCCTCACCTTGCTTGCCGCGAGGTCTGTACAAGGCCAGGGCCCGTATTCACCCGGGCTATAGGACCCACGactactgcagcttcaacactcttggatcatattgtc > 1:1325252/1‑270 (MQ=2) | ACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGC > NC_000913/3427092‑3427447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |