BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	731,077	A→G	8.5%	T499A (ACT→GCT)	rhsC →	Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	731,077	0	A	G	8.5%	298.3 / 21.0	146	T499A (ACT→GCT)	rhsC	Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand): ref base A (66/64); new base G (7/6); total (75/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

Only 100 of 146 total aligned reads displayed.

CGCAGGGTGAAGGCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCGGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATAC  >  NC_000913/730784‑731370

cGCAGGGTGAAGGCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCGGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCAGCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGAAGACTGGGGCCCGCTGATTCAGGAAACTGccc                                                                                                                                                                                                                                                                                                   >  2:1450038/1‑300 (MQ=255)

 gcgggggGAGGGGGGGGTGAAGTGGGGGGTGAAAAAGGGAGAGGCTGAGGGCAGCTCCAGGCAAGGCCAGGTTGACGCGTTGGGCAGGCTCAGGGCACAGACGGCTGCCGCAGGCAGGACAACAGAATGCAGTCCGGATGTGGTGACGGGCCTCACCACGCGCATCAACACGCCGGATGGCAGGGCATCGGCGTTTTACGATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGcccc                                                                                                                                                                                                                                                                                                  <  1:1284294/297‑1 (MQ=14)

                 gggAAGGGGGGGGGAAAAAAAGAACACCGGGACGGCAGGGTAAGGGGAGGTCAGTGTGGCGCGGTGGGCAGGCTGAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGCAGGGCCTCAGCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCAcc                                                                                                                                                                                                                                                                                  <  1:1449995/298‑1 (MQ=14)

                       cGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCGGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGCCGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGAACCAACCCGCTAc                                                                                                                                                                                                                                                                            >  2:590982/1‑300 (MQ=255)

                            ggtgAAAAAGGAAGCGCCGGACGGAAGCGTCACGCAGAGGCAGGTTGACGCGGTGGGCAGGCGCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTa                                                                                                                                                                                                                                                                       <  1:503987/300‑1 (MQ=255)

                               gAAAAAGGAAACCGCGGAAGGCAACGTCACTAAGGGTCAGTGTGACGGGGTGGGCAGGCGCAGGGAAAAGACGGATGGCGCATGCAGGACAACAGAATCACGTCCGGATGTGGGGACGGGCCGCATCACGCGCATCACCACGCCGGATGGCAGGTCATCGGCGGTTTACTATAACCACTACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAGCTGCCCCTGACGGCGAGATCACCCGCTACCGTTATGa                                                                                                                                                                                                                                                                    <  1:1166393/300‑1 (MQ=14)

                                aaaaaGGAACACGCGTACGGCAGCGTCACGCAGAGTCAGTTTGAAGAGGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGGGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGAt                                                                                                                                                                                                                                                                   <  2:1544863/300‑1 (MQ=255)

                                   aaGGAACACGCGGAGGGGAGCGGCACGGGGTGGAAGTTTGAAGGGGTGGGCAGGCGCAGGGCACAGAGGGAGGACGCAGGCAGGACAACAGAATACAGTCGGGATGTGGTGCCGGGCCTCATCACGGGCATCACCACGCCGGAGGGCAGGGCAGCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATc                                                                                                                                                                                                                                                               <  1:1180208/301‑1 (MQ=14)

                                         ggtaagagtggtataaaggccagggtgcAGGTTGACGCGGTGGCCAGGCTCGGGGCACGGCCGGATGCCGCAGGCAGGACAACCGAATACAGGCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCcacac                                                                                                                                                                                                                                                          <  1:356375/273‑1 (MQ=11)

                                          acgcggacggggggcaaaCGCAGAGGCAGTTGGACGGGGTGGGCAGGGTCAGGGCACAGACGGATGCCGCAGGCAGGAAAACAGAATACAGGCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑g                                                                                                                                                                                                                                                       <  1:590974/284‑1 (MQ=11)

                                           cgcgGACGGCAGCGTCACGCAGAGTCAGTTTGACGCGGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGACTGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTCTGCTAATCCACACA‑Gt                                                                                                                                                                                                                                                      >  2:291844/1‑301 (MQ=255)

                                                 cGGCAGTGCCACGAAGAGGGAGTTGGACGCGGGTGGCAGGCCCAGGGCACAGACGGATGCCGCAGGCAAGACAACAGATTACAGGCCGGATGTGGTGACGGGCCGCATCACGCGTATCACCAAGCCGGATGGCAGGGCATCGGCGGTTTACGATAACCACCACAGCCAGTTAACGTCAGCCAGCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAGCTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACtt                                                                                                                                                                                                                                                 <  2:107465/300‑1 (MQ=11)

                                                   caagcgtaacgcacgttaaggtggACGCGGTGGGCAGGCGAAGGGAAAGGACGGAGGCCGCAGGAAGGACAACAGAATACAGGCCGGATGTGGGGACGGGCATAATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGGTGTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTAc                                                                                                                                                                                                                                               <  1:291838/277‑1 (MQ=11)

                                                    cggggacaagCAGAGGCACTTTGCGGGGGTGGGCAGGCGCAGGGAGCAGACGGAGGACGGAGGCAGGACAACGTAGTACAGTCCGGCGGTGGGGAGGGGACTCATAACGCGCATCACCACGCCGGATGGGAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTGAACGTCAGCCACCGGGGCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATGCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAAGCCACACA‑GTGACTTAccc                                                                                                                                                                                                                                             <  1:881121/292‑1 (MQ=11)

                                                     gagacgggggagggtggttgtGACGCGGTGGGGAGACGCAGGGCACAGACGGAGGCCGCAGGCAGGACAACAGAATACAGTCCGGAAGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGTTGGCATGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTAccc                                                                                                                                                                                                                                             <  1:612380/280‑1 (MQ=14)

                                                            cGCAGAGTCAGTTTGACGCGGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATTACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAATCACCACAGCCAGTTAACGTCAGCCACCCGGCCTGACGGGCTGGAAATACCCCGGGCATATGATGAATGGTGCCGTCTGATTCAGGAAAATTCCTCTTACGGCGATATCACCCGCTACACTTCTGATCATTCACACA‑GTGACTTAcccctgtcc                                                                                                                                                                                                                                       >  2:612388/1‑293 (MQ=14)

                                                                gagTCAGTTTGACGCGGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCcacac                                                                                                                                                                                                                                                          >  2:356382/1‑277 (MQ=39)

                                                                                  gggCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGACTGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAccccg                                                                                                                                                                                                               >  2:1171491/1‑299 (MQ=38)

                                                                                  gggCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTCCCCCTGCCGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGTAAGATGACACCGGCAGCCgg                                                                                                                                                                                                               >  2:1069505/1‑301 (MQ=255)

                                                                                       ggCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCCGGAAACTGCCCCTGACGGCGATATCACCCGCTCCCGTTATGATAATCCACACA‑GTGACTTACCCTGCCCCACGGAAGCTGCCCCCGGCACCCGGaaaaa                                                                                                                                                                                                          >  1:377808/1‑300 (MQ=255)

                                                                                        gCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTGATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGGAAATGccctggcccttataaacata                                                                                                                                                                                                         >  2:434911/1‑283 (MQ=255)

                                                                                             gggCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCCGCCACCGGGCCTGACGGGCTGGACATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATCACCACCGCTACCGTTATGATAATCCCCACA‑GTGACCTACCCTCCGCCACGGGCGATGCCACCCGCCCCCGGCAAACAATgc                                                                                                                                                                                                     >  1:850007/1‑299 (MQ=11)

                                                                                             gggCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGCACTACGCCGGGAATATGATGAATGGGGCCGTCTGATTCCGGAACCTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACCCC‑GTGACTTACCCTCCGCCACGGAAGATGCCACCGGCCACCGGAAAAcccaga                                                                                                                                                                                                     >  1:1546750/1‑296 (MQ=11)

                                                                                               gCACAGAAGGAGGGCGGAGGCAGGACAACAGAATAAAGTCCGGAGGTGGGGACGGGCCTCAGCACGCGGATCACCACGCCGGATGGAAGGGCATCGGCGTTTTAATATAACCACCACAGCCAGTGAACGTCAGCCACCGGGCCCGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACg                                                                                                                                                                                                   <  1:1538433/300‑1 (MQ=11)

                                                                                                    gACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAACTACGGCGGGAATATGATGAATGGGGCCGGCGGGTTCAGGAAACTGCCCCTGACGGCGGTAGCACCCGCCACCCTTATGATAAGCCACACA‑ACGCACTCCCCCGCGCCACCGAAGATGCCACCAGCAGCACGAAAACCaacgacgctaaa                                                                                                                                                                                             >  1:26328/1‑290 (MQ=11)

                                                                                                                aggcaggACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTa                                                                                                                                                                                        >  2:1175008/1‑294 (MQ=38)

                                                                                                                 ggcaggACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCAAGCGCATCACCACGCCGGATAGCAGGGAATCGGAGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCAAACGGGCCTGACGGGCTGGAAATAAGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACGGCCCCTGACGGCGATATCAACCGCTACCGTTATGCTAATCCACACA‑GTGGCTTACCCTGAGCAACGGACGGTGCCACCGACAGCCGGCACACCCTGACATGGCGCCtttgacgtcaga                                                                                                                                                                                >  2:598996/1‑292 (MQ=255)

                                                                                                                     ggACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCCGGGCATCGGCGTTTTACTATAACCACCACAGCCCGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGCATATGCTGAATGGGGCCGTCTGCTTCAGGAAACTACCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCCACGGAAGATTCACCCGGCAGCCGGAACACCCTTACGTAGAGCCGtggcgggacggtgc                                                                                                                                                                             >  2:106136/1‑287 (MQ=11)

                                                                                                                     ggACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTCTGATAATCCACACA‑GTGACTTACCCTTCCCAACGGCAGATTCCACCGGCAGCCGGAAAACCATGACCGGGGGCCGTTCAGGTCAGGTGc                                                                                                                                                                             >  2:5194/1‑300 (MQ=11)

                                                                                                                     ggACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATAGGCGTTTTACTATAACCACCACAGCCAGTTAACTTCAGCCACCCGGCCTGACGGGCTGGAAATCCGCCGTGAATATGATGAATCGGGCCGTCTGATTCAGGAAACTGCCCCTGCCGGCGATATCACCCGCTACCGTTATGATACTCCACACA‑GTGACGTACCCTGCCCAACGGAAGATACCCCCGGCAGCCAGAAAACCATGCCTTGGAGACGTTACTGTCAATTGca                                                                                                                                                                            >  2:37520/1‑300 (MQ=255)

                                                                                                                       acaacaGAATACCGTCCGGATGGGGGGACGGGCCGCATCACGCGCATCACCCCGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTg                                                                                                                                                                           <  2:1546794/300‑1 (MQ=255)

                                                                                                                        caacaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGat                                                                                                                                                                                                                                                                                        >  1:1247003/1‑191 (MQ=255)

                                                                                                                        caaaaGAATACAGTCCGGATGTGGTGAAGGGCCGCATCACGCGCATCACCAAGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGat                                                                                                                                                                                                                                                                                        <  2:1247037/191‑1 (MQ=255)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATCACCACCCCAGCCAGTTAACGTCAGCCACCGGGTCTGCCGAGCTGGAAATACGTCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGACACTGACGGCGATTTCACCCGATTACGTTCTGATAAT‑TACCCACGTCACTTATCCTGCGCCACGGACGATGCTGCCGGCAGCCGGACAAACATGACGTGGAGCCGGGACGGTCAGTTGcacgg                                                                                                                                                                         >  2:187047/1‑295 (MQ=11)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAGCTGCCCCTGCCGGCCATATCACCCGCCACCGTTATGATAATCCACACA‑GTGACTTACCCTGCCCCACGGCAGATCCCCCGCGCAGACGGAAACACATGACGTAGCGCCCGTCCGGTCAGTTCCTGAGcg                                                                                                                                                                       >  1:1013568/1‑300 (MQ=11)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCCCCCGCTACCGTTATGATAATCCCCACA‑GTGACTTACCCTGCGCAACGGCAGATGCCACCGGCCGCCGGAAAACCATGACCTGGCACCGTTACGGCCAGTTGCTggtcg                                                                                                                                                                       >  1:1461772/1‑297 (MQ=255)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGCTAATCCACACA‑ATGACTTACCCTGCGCAACGGAAGATGCCACCTGCACCCGGAAACACACAACCTGGGGCCCGTACGGTCAGTTGCttac                                                                                                                                                                         >  2:604035/1‑296 (MQ=11)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGAt                                                                                                                                                                                                                                                                   <  2:174448/210‑1 (MQ=255)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGAt                                                                                                                                                                                                                                                                   >  1:174444/1‑210 (MQ=255)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTCCCCTGCGCAACGGAAGATGCCACCGGCCACCGGAAAACCATGACGTGGACCCGTTACGGTCAGTTTCTGAGcg                                                                                                                                                                       >  1:1488528/1‑300 (MQ=255)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTAc                                                                                                                                                                                                                                               >  2:110014/1‑229 (MQ=40)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGGTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATCCAGGAAACTGCACATGACGGCCATATCACCCGCTACCGGTATGATAATCCACACA‑GTGACTTAACCTGCGCAACGGAAGATGCCACCGGCAGACGGAAACACATGCAGCGGGGCCGTTCCGCTCAGTTGCTGAggt                                                                                                                                                                       >  2:426197/1‑299 (MQ=11)

                                                                                                                          acaGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCAACACCGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGCATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTCCCGTTATGATAATCCACCCA‑GTGACTTACCCTGCACAACGGAAGAGGCGAACGGCGACAGGAAAAAAGTGACGGGGCGGCTTTACTGGCAGGTGGTGAg                                                                                                                                                                         >  2:1238003/1‑299 (MQ=11)

                                                                                                                           cagaacacgttccggggtgggtgGCGGGCCTCAGCACGCGCATAACCACGCCGGACGGAAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATCCGCCGGGAATAGGATGAATGGGGCCGTCTGATTCAGGAAGCTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTc                                                                                                                                                                                   <  1:1242196/269‑1 (MQ=11)

                                                                                                                             gAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGCAATACGCCGGGAATATGATTAATGGGGCCGTATGATTCAGGAAACTGGCCCTGAGGGCGATATCACCAGCTACCGTTATTATAATCCCCACA‑GTGACTTACACTGCGCAACGGAAGATGCCACCGGACACAGGACAACAGTGAAGTGGAGCCGTGTCCGCCACTTGCTggagtgca                                                                                                                                                                    >  2:1495323/1‑294 (MQ=11)

                                                                                                                                    gTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCCGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGACTGGGGCCGTCTGATTCAGGAAACTTCCCCTTACGGCGATATCACCCGCTACCGTTATGCTAATCCCCCCC‑GTGACTTACCCTGCGCAACGGACGATGCCACCGGCAGCCGGAAAACCATGACATGGGCCCCTTCCGGTCGGTTGCTgccgttccctca                                                                                                                                                                >  1:1414998/1‑287 (MQ=11)

                                                                                                                                    gTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATGTGATGCATGGGGCCGTCTGATTCCGGAAACTGCCCCTGACGTCCATATGACCCGCTACCGTTATGATCATCCACACA‑GTGACTTCCCCTGCCCAACAGAGAATGCCCCCAGCACCCCGAACACCCTTACTCGGAGCCGCTActgcccgttagttttcttgagtgactt                                                                                                                                                             >  2:1281610/1‑275 (MQ=11)

                                                                                                                                    gTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATCATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGGCGTGGAGCCGTTACGGGCAGTTGCTGAGCTTCactgcctt                                                                                                                                                             >  2:1018650/1‑300 (MQ=255)

                                                                                                                                            gtggtgACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCcac                                                                                                                                                                                                                                                            >  1:730054/1‑199 (MQ=38)

                                                                                                                                            gtggtgACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACC‑GTGACTTAccc                                                                                                                                                                                                                                             >  1:139712/1‑213 (MQ=38)

                                                                                                                                            gtggtgACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGCCGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCCCTGACTGTTCCCGtt                                                                                                                                                     >  2:863739/1‑301 (MQ=25)

                                                                                                                                              ggtgACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGGTATGAGCATCCACACA‑TGTACTTACCCATCGCCCCGGACGCTTCCACCAGCCGCCGGCATACACTGCCCTGGCTCCGTTACGCTCCTTTGCTCACCTTCCCTGACTGTTCCgggtca                                                                                                                                                   >  1:1012137/1‑297 (MQ=11)

                                                                                                                                                  ccggccctccaacacgcgaccaccacGACGGATGGCATGGCATCGTCGTTTTAAGATAACCACCACAGCCAGTTAAGGGCAGACAACGGGCTTGACGGGCTGGAAATACGCCGGGAATATGAGGAAGGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTaa                                                                                                                                               <  1:1065550/281‑1 (MQ=11)

                                                                                                                                                      gctgcagcaCTCGCATAACCACGCCGGATGGAAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGTGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGAt                                                                                                                                                                                                                                                                   <  1:1358545/175‑1 (MQ=11)

                                                                                                                                                      gCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGCTTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCATTATCAt                                                                                                                                                                                                                                                                   >  2:1358585/1‑182 (MQ=255)

                                                                                                                                                             caCGCGCATCACCACGCCGGAGGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGTCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTatgac                                                                                                                                    <  2:1488572/301‑1 (MQ=38)

                                                                                                                                                              cacccaaTCACCACCGCGGATGGCACGGCAGCGGCGTTTTACTATCACCACCAACGCCAGTTAACGTCAGACACCGGGCCTGACGGGCTGGAAATACGCCGTGAATATGATTAATGGGTCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTatgacc                                                                                                                                   <  1:655563/295‑1 (MQ=11)

                                                                                                                                                               cgcgCATCGCCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGACACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATCATCCAAACA‑GTGACTGACCCTGCGCAACGGCAGATGCCACCGGCAGCCGGCAAACCATGACGTGGAGCCGGTACGGTCAGTTGCTAAGATTCCCTGACTGTTCCGGTATTGTAACACGCTctgaaca                                                                                                                                  >  2:1154260/1‑301 (MQ=11)

                                                                                                                                                               cgcgCATCACCACGCCAGATGGAGTGGCAACGGCGTTTTAACAGAACCACCACCGCGAGTGCACGCCAGCCACCGGGGCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGACAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCTCACA‑GTGCCTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTatgacca                                                                                                                                  <  2:970042/301‑1 (MQ=11)

                                                                                                                                                                                    gCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAGCTGCCCCTGACGGCGCTATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTCAGCTTCCCTGACTGGTCCGGTTATGTAACCCGCTATGACCCTCACCGGTTTGTGCAGGTGa                                                                                                              >  2:787279/1‑300 (MQ=11)

                                                                                                                                                                                    gCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAGCTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTCTGACCCTGCCCGTTTTGGTCCGGTGa                                                                                                              >  2:971770/1‑300 (MQ=255)

                                                                                                                                                                                       gggCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAGCTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCACCGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTTTTCCGGTTATGTAACCCGCTCTGACCATGACCGTTTTGGTCAgtggccggg                                                                                                          >  1:270650/1‑293 (MQ=255)

                                                                                                                                                                                       gggCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGCTATCACCCGCTACCGTTATGATAATCCACACA‑GTGCCTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTc                                                                                                                                                                                   >  2:1410677/1‑228 (MQ=25)

                                                                                                                                                                                        ggCCTCGGCGTTTTACTATAACCACCAAAGCAAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGAcggc                                                                                                          <  2:1378833/300‑1 (MQ=255)

                                                                                                                                                                                               gCGTCTTACTATAACCACGACATGCAATTAACGTCAGCCGCCGGGCCTGACTGGCCGGGAATACGCCTGTAATATGATGAATGGGGCCGTCTGAGTCAGGGAACTGCCCATGACGGCGATCTGACCCGCTACCGTTATGATAAGCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGACGGAAACCGATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGGTTTGGTCAGGTGACGGCGGTGCAc                                                                                                   <  2:49145/300‑1 (MQ=14)

                                                                                                                                                                                                      ccTATAACCAACGCGGCAAGTTAACGTAAGCGACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAGCTGCCCCGGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTGACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGa                                                                                           <  1:1140456/300‑1 (MQ=255)

                                                                                                                                                                                                      aaaataACCCCCGCCGTCAGGTAACGTCAGCCACCGGGCCGGACGGGCTGGAAAGACGCCGGGAATATGATGAATGGGGCCGTCGGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCGACCGTTAGTATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGAGGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGa                                                                                           <  1:1312940/298‑1 (MQ=14)

                                                                                                                                                                                                       cTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCACAACGGAAGATGGCACCGGCAGCCGGAGAACCATGACGTGGAGCCGTTACGGGCAGTTGCTGAGCTTCACTGGCTGTTACGGTTATGTAACCCGCTATGACCATGACCGTTGGGGTCCGGTGGCGGCGGTTCACGGCGAGGag                                                                                          >  2:490373/1‑300 (MQ=14)

                                                                                                                                                                                                         ataACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCCCCGGCAGCCGGAAAACCATGCCGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGCCTGTTCCGGTTATGTAACCCGCTATGCCCATGCCCGGTTTGTTCAGGTGACGGCGGTGCCCCGCGCGGGAgg                                                                                        >  2:710440/1‑301 (MQ=255)

                                                                                                                                                                                                                      ccAGTTAAAGTCAGCCACCGGGCCTGACGGGGTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGAATCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑c                                                                          <  2:1380403/301‑1 (MQ=255)

                                                                                                                                                                                                                      ccAGTGAGCGTCAGCCACCGGGCCGGAGCGGCTGGGAACACGCCGGGAATATGCTGAATGGGGCAGGCTGATTAAGTAAACTGCCCCTGACGGCGCTATCACCCGCTACCGTGATGATAAGCCACACA‑GGTACGGACCCGGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTGACGGTCAGTTGCTGGGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑c                                                                          <  1:1479735/301‑1 (MQ=14)

                                                                                                                                                                                                                      aaaGTTAACTGCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGGTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑c                                                                          <  2:1461816/299‑1 (MQ=255)

                                                                                                                                                                                                                       cAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCAACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCGCTGACTGTTCCGGTTATGTAACCCGCTCTGACCATGAACGTTTTGGTCAGGTGACAGGGGTGCACCGCGAGGACGGTGTGGGGCAGTA‑c                                                                          >  2:104064/1‑300 (MQ=14)

                                                                                                                                                                                                                             aCGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCGTCACTGACTGTTCCGGTTATGTAACCCGCGATGACCATGACCGTTTTGGTAAGGTGGCGGCGGTGCACAGCGCGGAGGGGCTGGGTCAGTA‑CCGGGCAt                                                                   >  2:732773/1‑301 (MQ=255)

                                                                                                                                                                                                                                 catccACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGAGAGCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTc                                                                                                                    <  1:1279127/246‑1 (MQ=255)

                                                                                                                                                                                                                                     caacGGGGCTGTCGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTTGGATTACGTAAACTGCCCCTGACGGGGCTATCACCCCCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGc                                                           <  1:598289/298‑1 (MQ=14)

                                                                                                                                                                                                                                     cACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCCCCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGACGGGCTGAGGCAGTA‑CCGCGCATACGAccg                                                            >  2:1234279/1‑298 (MQ=255)

                                                                                                                                                                                                                                          ggCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCAAACA‑GTGACTTACCATGCGCAACGGAAGATGCCAACGGCAGCCGGAAAACCCTGACGTGGAGCCGTTACGGTCAGTAGCTGAGCTTCACTGACTGTTCCGGTTATGTAATCCGCTATTACCATGCACGGGTTGGTCCAGTTAAGGCGGGGCCGCGCCAGGAAGGGCTGCGGCAATA‑CAGCGGCTACGCCCGCCGTgg                                                      >  2:712430/1‑301 (MQ=11)

                                                                                                                                                                                                                                           gcggatcGGGGTGGAAATACGCCGGGAATAAGAGTCATGTGGCCGGCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCACCTACCTTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGTTGCCACCGGCAGCCGGAAAACCAGGACTTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTAGGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTgg                                                      <  1:712421/294‑1 (MQ=14)

                                                                                                                                                                                                                                            ccggACGGGCTGGAAATACGCCGGGAATAGGATGAATGGGGCCGTCTGATGCAGGAAGCTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTGACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGAc                                                    <  2:270657/298‑1 (MQ=255)

                                                                                                                                                                                                                                            ccTTACGGGCTGGAAATACGCCGGTAATAGGATGAATGGGGCCGTCTGATTGAGGAAACGGCCCCTTACGGCGAGATCAGCCGCGACCGTTAGGATAGTCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGAc                                                    <  2:1331928/301‑1 (MQ=14)

                                                                                                                                                                                                                                                 cgggtgggaaaatggacGGGAAGAGGATGAATGGGGCCGGCTGATTGAGGAAACTGCCCCTTAAGGCGATATCACCCGCTACCGTTATGATAATACACACA‑GGGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGGCGGAAAACCATGGCGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTa                                               <  1:1018622/285‑1 (MQ=11)

                                                                                                                                                                                                                                                   ggCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGACGATGTCACCGGCAGCCGGAAAAACATGACGTGGCGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGGTCCGGTTATGTAACCCGCTATCACCATGACAGCTTTGGTCAGGTGACGGCGGTGCACCGCGGGGACGGGCTGAGCCAGTACCCGCGC‑TACGAAGGCCGTGGACAGttgaa                                             >  1:1089487/1‑298 (MQ=11)

                                                                                                                                                                                                                                                   ggCTGGAAATACGCCGGGAATATGATGAATGGGGCAGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGCGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTCACCCGCTCTGACCATGACCGTTTTGGTCAGGTGACGGCGTGGCACCGCGCGGAAGGGATGATTCATAA‑CCTCGCATTCGGCGGCCGTGGGCCGTTAAt                                             >  2:1000691/1‑301 (MQ=14)

                                                                                                                                                                                                                                                      tGGAAATACGGCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGc                                          <  2:26329/301‑1 (MQ=40)

                                                                                                                                                                                                                                                        gAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCTCCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGCGCTTCACTGACTGTTCCGGtt                                                                                                                                                     >  1:1163114/1‑193 (MQ=37)

                                                                                                                                                                                                                                                        gAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCCGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATTACCCTGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGACGGGCTGAGGCCGTA‑GCGCGCCTCCGCCCGCCGGGGCCAGGTAATTACCg                                        >  1:1246645/1‑301 (MQ=14)

                                                                                                                                                                                                                                                               gCCGGGAATAGGAGGAATGGGGCCGTCGGATGCAGGAAACTTCCCCGGACGGCGAGATCAACCGCTACCGTTATGAGAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGGTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAg                                  <  1:1538851/300‑1 (MQ=11)

                                                                                                                                                                                                                                                                   ggAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAAACATGACGTGGAGCCGTTACGGTCCGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCGGGC‑CCGCCCAGACGACAGAGGAGGACGGTGAAGCGCCGTGAAAGaaaag                             >  1:817288/1‑297 (MQ=11)

                                                                                                                                                                                                                                                                    gAATATGATGAATGGGGCCGTCTGATTCAGGGAACTGCCCCTGACGGCGATATCACCAGCTAACGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTGAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGACAGGTGACGGCGGTGCACCGCGAGGAAGAGCTGAGTCAGTA‑CCGCGCATACGACCGCCGTGGCCAGTTAATTGCCGTGACACACACg                             >  2:115860/1‑300 (MQ=11)

                                                                                                                                                                                                                                                                    gAATATGAGGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑c                                                                          <  2:1396698/255‑1 (MQ=39)

                                                                                                                                                                                                                                                                        atgatgAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTTACTGTTCCGGTTATGTAACCCGCTCTGACCATGACCGCTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAGGGCCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTATTTGCCGTGAACGaccccccggg                        >  1:863170/1‑293 (MQ=255)

                                                                                                                                                                                                                                                                             gaATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATTACCATGACCGTTTTGGTCCGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCCGTA‑CCGAGCATACGACCTGCGTGGCCAGTTACTTGCCGTGAAAGACACCCAgggaccgg                   >  1:1192055/1‑296 (MQ=11)

                                                                                                                                                                                                                                                                              ccgtgggCCGTCCACTTCAGTAAACTTCCCCTTACGGCAAAATCACCCGTCACCGTTATGATAACCCCAACA‑GGTACTGACCCTGCGCAACCGAAGATGCCACCGGCAGCCGGAAAACAAGGACGTGGAGCCGTGACGGTCATTTGCTGAGCTTCACGTACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTGTGTCAGGTGACGGCGGTGCACCGCGAGTAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGa                  <  1:479755/297‑1 (MQ=11)

                                                                                                                                                                                                                                                                                 ggggCCCTCGGATGCAGGAATCTGCCCCGGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGaaa                <  2:863181/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                             ttCAGGTAACGTCCCCTGACTGCGATATCCCACGCGACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGTAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTACGGTTATGTAACCCGCGATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATa   <  2:808394/301‑1 (MQ=255)

                                                                                                                                                                                                                                                                                              tCAGGCAACTGCCCCCGCCTGCGTAAACCCCCGCTACGGTTATCATAATCCCCACA‑GTGACTTCCCCTGCGCCACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGGGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGTTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATAc  <  2:817300/301‑1 (MQ=11)

                                                                                                                                                                                                                                                                                              tCAGGAAACTGCCCCTGAAGGCGATACCAACCGCTACCGTTATGATAATCCACACA‑GGGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATAc  <  2:1246679/301‑1 (MQ=25)

                                                                                                                                                                                                                                                                                              caaGGAAACAGCCCCTTAAGGTGAGATCACCCGCATCCGTTATGATAATCCAAAAA‑GTGACGTACCCTTCGCAACGGAAGAGGCCAACGGCAGCCGGAAAACCATGACGGGGAGCCGGTACGGTCAGTGGCTGGGCTTCACTGCCTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATAc  <  1:104061/299‑1 (MQ=11)

                                                                                                                                                                                                                                                                                               cAGAAAACCGCCCCCGACGGGGTGATAACACAGTACCGTTAGGAGAAGCCACAAA‑GTGACTTACCGTGCGCAACGGAAGAGTCCACCGGCAGCCGGAAACCCATGACGTGGAGCCGGTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGGCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATAc  <  1:673376/300‑1 (MQ=11)

CGCAGGGTGAAGGCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCGGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAATACAGTCCGGATGTGGTGACGGGCCTCATCACGCGCATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACA‑GTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACTGACTGTTCCGGTTATGTAACCCGCTATGACCATGACCGTTTTGGTCAGGTGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTA‑CCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATAC  >  NC_000913/730784‑731370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`