mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | SSW-KHP-SSW-1-163-1-1_S1_L001_R2_001 | 1,556,130 | 467,227,792 | 99.9% | 300.2 bases | 301 bases | 82.3% |
| errors | SSW-KHP-SSW-1-163-1-1_S1_L001_R1_001 | 1,558,105 | 468,314,432 | 100.0% | 300.6 bases | 301 bases | 85.0% |
| total | 3,114,235 | 935,542,224 | 99.9% | 300.4 bases | 301 bases | 83.6% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 166.6 | 2.5 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 10789 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 36 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.005 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.80503 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.8 |
| Consensus minimum coverage each strand | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum coverage each strand | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 02:12:44 06 Mar 2016 | 02:14:22 06 Mar 2016 | 1 minute 38 seconds |
| Read alignment to reference genome | 02:14:23 06 Mar 2016 | 02:21:28 06 Mar 2016 | 7 minutes 5 seconds |
| Preprocessing alignments for candidate junction identification | 02:21:28 06 Mar 2016 | 02:23:01 06 Mar 2016 | 1 minute 33 seconds |
| Preliminary analysis of coverage distribution | 02:23:01 06 Mar 2016 | 02:27:06 06 Mar 2016 | 4 minutes 5 seconds |
| Identifying junction candidates | 02:27:06 06 Mar 2016 | 02:27:09 06 Mar 2016 | 3 seconds |
| Re-alignment to junction candidates | 02:27:09 06 Mar 2016 | 02:27:52 06 Mar 2016 | 43 seconds |
| Resolving alignments with junction candidates | 02:27:52 06 Mar 2016 | 02:31:50 06 Mar 2016 | 3 minutes 58 seconds |
| Creating BAM files | 02:31:50 06 Mar 2016 | 02:34:14 06 Mar 2016 | 2 minutes 24 seconds |
| Tabulating error counts | 02:34:14 06 Mar 2016 | 02:38:28 06 Mar 2016 | 4 minutes 14 seconds |
| Re-calibrating base error rates | 02:38:28 06 Mar 2016 | 02:38:29 06 Mar 2016 | 1 second |
| Examining read alignment evidence | 02:38:29 06 Mar 2016 | 03:09:05 06 Mar 2016 | 30 minutes 36 seconds |
| Polymorphism statistics | 03:09:05 06 Mar 2016 | 03:09:06 06 Mar 2016 | 1 second |
| Output | 03:09:06 06 Mar 2016 | 03:09:58 06 Mar 2016 | 52 seconds |
| Total | 57 minutes 13 seconds | ||
