Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	382,305	0	T→G	100.0%	0.6	3	F211C (TTC→TGC)	insD1	IS2 transposase TnpB
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/2):  ref base (0/1):  total (0/3)

ACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGC  >  NC_000913/382295‑382604           |                                                                                                                                                                                                                                                                                                            acacGCCAGGTCGCCCGGATGTGGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCGAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTAc           <  1:81510/301‑1 (MQ=11)  cacGCCAGTGAGACCGGATGTTGGGACCTCAACCGAAGAAACGGGAGGGGAGGAGGCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACACCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCGTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCAACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTAcc          <  1:81550/301‑1 (MQ=11)  caagCCAGTGGGACCGGATGGTGGGACGTGAAACGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAAAAGCAGAGAGCTTCGTGAAAACGAGAAAGCGTGACTACATCAGGATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTAcc          <  1:345595/298‑1 (MQ=255)          ttCGCCCGGAGGGTGGGGGGTGAGCAGCAGAACAGGGCGGGGCGGAGTCAGGAGAGCAACGGGAGAGCAGGTAGCTTAGTGAAAGCGATACAGAGCGACTACGCCAGTATCCTGCCCAAGCTAGACGGCTGAACGGCAGCAAAGAACCGTGAGGATGAGTTCGAGGACGAGAACGAATGGCAGCAGCATGGGGCGCTGGGTTATCGGTCGCCGCCGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATACAGGGGCAAATCCAATTACCTATCAGGc  <  1:524261/301‑1 (MQ=2)           |                                                                                                                                                                                                                                                                                                            ACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGC  >  NC_000913/382295‑382604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.