| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 657,541 | G→T | 53.9% | intergenic (+40/+14) | cspE → / ← flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Read alignment evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | change | freq | score | reads | annotation | genes | product | ||
| * | NC_000913 | 657,541 | 0 | G→T | 53.9% | 131.5 ‑1.6 0.0 | 119 | intergenic (+40/+14) | cspE/flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Reads supporting (aligned to +/- strand): new base (30/34): ref base (32/22): total (63/56) | ||||||||||
| Fisher's exact test strand distribution p-value = 1.99e-01 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00 | ||||||||||
Only 100 of 120 total aligned reads displayed.
GGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAAC > NC_000913/657243‑657837 | ggTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAACCCCGCTTAATCAGCGG‑T‑tt > 2:642433/1‑301 (MQ=255) gACACAGCATGTGTGTCTATTTTTGATGGAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTCAGTGGTTTGATTGATGCAAAGGAGGCGGGTTCATTACTCCGGAGGACGGCAGCAAAGACATGTTCGTACACGTCTCGGCACACCAGACGAATGGTTTTAAAAATCTTGCTGAAGGTCAGCGCGTAGAGGTCGAGATCGCTAAAAGTGACAAGGGCCGTTCTGCGGCAGACGTACTGGCGCTGTAGTCTAGGACAGAGAGAATTCAAACCACCCTAAATCAGCTG‑C‑ATTTTTTTg > 1:297008/1‑301 (MQ=255) acacAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGACGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCGCTGTAAGATACGTCAGCAAGACTTCAAAACCGGATTAATACGCGG‑T‑TTTTTTTTgg > 2:512569/1‑301 (MQ=255) caGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGTGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGCTTTAAAACTCTTGCTGAAGTTCAGCGCGTAGAGTTCGAAATCACTAACGGTGTCAAAGGCCCTTCTGCTGCACACGTAATCGCCCCGTAAGATCAGTCAGCAACAATTACAACCCCGCTTAACTAACGG‑G‑TTTTTTTTgtg > 1:83558/1‑297 (MQ=255) gCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGACAACGGCCCTTCTGCTGCAAACGTAATCGCACTGTACGATACGTCAGCAAGAATTCAAAACCCGCTTCATCAGCGG‑G‑ATTTTTTTGGTCttt > 2:295487/1‑301 (MQ=255) ttttGTGTCTATTTGTCATTTAAAGGGGAGTTTGAAGTGTAAGATGAAAGATAACGTTAAGGGGTTTGAAGAGTCCCAAGGACTCGGTCTCACTACTACGGAAGACGGCAGCAAAGACGGGCGCGTACACTTCTCTGCAATCCAAACTAATGGCTTTAAAACTCGGGCTTAAGGTTACCGCGTAGAGTTCGAAATCACTACCGGTGCCAAATGCCCTTCTGCCGCAAACGTAACAGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTCATCAGCGG‑T‑TTTTTGGTGGTCTTTAg < 1:875079/300‑1 (MQ=255) ttgtgttgcgagtttgaaTGGAAAGGTAATTTGGATGGCTAAGATTAAAGGTAATGGTAAGTGGTTTAATGAGTCCAAAGGAGTCGGTTTCAGTACCACGGAAGACGGCATCAAAGACGTGTTCGTAAACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAg < 1:634220/284‑1 (MQ=255) aTTTGTGTCTATTGTTCATGTAAATGTAATTTTGATTTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGGCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGGCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAg < 2:899109/301‑1 (MQ=255) aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTg > 2:275532/1‑301 (MQ=255) gtGTAAAGGGAATTTTGATGTCGAAGAGTAAAGATAACGTTGAGTGGTTTCATGAGTACAAAGGATGCGGTTTCATTACTCCGGAAGACGTCAGTAAAGACGTGTTCGTACCCTTCTCTGCACTCCAGAATAATGGATTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAACCGTACTCGCTCTGTAAGATACGTCAGCCAGAATTCAACACACGCTTCCTCAACAG‑T‑TCTTTTTTGATCTTTatgttatgtttacggcgga > 1:600829/2‑283 (MQ=255) agagcTAATATTAAAGGTACCGTTAAGTGGTTTAATGAGTCCAAAGGAGTCGGTTTAATTACTCCGGAAGACGGCAGCAAAGACGTGCTCGGACACGTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATAACGAACGGTGATAAAGGTCCTGCGGCGACAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAACCCCGCTTAATCAGCGG‑T‑GTTCTTTTGGTCTCTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGcc < 2:120561/296‑1 (MQ=255) tCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGGCCAAAGGATTCGGTTGCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTg < 2:135964/301‑1 (MQ=255) aTTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCAGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTc > 1:850705/1‑300 (MQ=255) aGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAACCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTTCGGTTGAGGCCGAAAACAGCCAAAATGCCaagccgttcctgcca > 1:106526/1‑287 (MQ=255) aacgggaaaggggtgaaTGAGTCAAAAGGATTCGGTTACAGTACACCGGAATACGGCAGCAAAGACGTTTTCGTACACGTTCAGGCAATCCAGACTAATGGTTTTAAAACTCTGGCGGAAGGGCAGCGCGTAGAGTTCGAAATCACGAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg < 1:329930/286‑1 (MQ=255) cGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCCATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCATTCTCCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCACAACCCGCTTCATACGCGG‑T‑TTTTTTTTGGTCTTTAGTTTACGGGTCAAGCCGCAAAACGCCACAATCACAGTTCGGTCATgcaacacgaa > 1:795055/1‑292 (MQ=255) ttAAGTTGTGTAAAGGTTCCAAAGGCATCGGTTTTATAAAGCCGAGAGACGGCAGCAAAGAACTTTGCGTATACTTCTCTTCAATCCAGCATAATGGTTTGAACACTCTGCCTTAAGGTCAGCGCGTAGAGTTCGAAAGCACTAACGGTGCCAAAGGCCCTCCTCCTGCAAATGTAACCGCTCTGTAAGATACGTCAGTAAGAATTCAAAACCCGCTTAATCAGCTG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc < 1:512554/300‑1 (MQ=255) tAAGTGGTTTAATGAGTCCAGAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAGGAATTCACAACCCGCTTACTCAGCGG‑G‑TTTTTTTTGTTCTTGAGTTTTCGGTGGAGGCCGAAAACGTCCACAATGCCAGTGCGGTCATTGCCACAGAccc > 1:269218/1‑300 (MQ=255) ggttAATGAGTCCAAAGGAGTCGGGGTAATAAATCAGGAAAACGGCAGAAAAGACGGGTTCGTAAACTGCTCGGCAATCCAGAATAATGGTTTTAAAACTCGTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGACCTTCGGCGGCAAACGTAAGCGCTCTGGAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TGTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg < 1:687324/298‑1 (MQ=255) tttAATTAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGCGGGCAGCAAAGACGTCTTCGCACAATTCTTTGCAAACCAGAATAAGGGTGTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGAGACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGt < 1:919315/301‑1 (MQ=255) gTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGTTCATTGCCAAAGCCCCCAGAAGGTTGACGcaa > 1:685547/1‑301 (MQ=255) gTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGACAGAATGCCAGTGCGGTCATTGCAAAAAACCCCAGAAGGTTGAAGaaa > 1:169345/1‑301 (MQ=255) gTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCGCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGGTCGGTTGAGGCCGAGAACCGCCAGCATGCCAGGTCGGTAATGGCAAAAGCCCCCAGCAGGTTGCCGaaa > 1:380671/1‑301 (MQ=255) gTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATAACTAAAGGTGCCCAGGGCCGTTCTGCTGCAAACGTAATCGCGCTGTAAGATACGTCAGCAAGAATTCAAAACACGCGAGATGAGCGG‑G‑TTTGATTGGGTATTTAGTGGGCTGTTGAGGCGGAAAAAAGACAGAATGCGAGTGAGGTGATGGCAAAAGACAACAGAAGGAGGACGaaa > 2:759115/1‑301 (MQ=255) aagATTCGTTTTCATTACGCAGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAAACACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTc < 2:169351/299‑1 (MQ=255) tttCGTTTTCATTACTCCGGAAGACGGCAGCAAAGACGTTTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAACCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGt < 1:133013/300‑1 (MQ=255) tCGGTTGCAATACACAGGATGACGGCAGCAAAGGCGTGTTCGTAAACTGCTCTGCAATCCAGACTAATGGTTTGAATGCGCTTGATTAATGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAACCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCTG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTaa < 2:797972/301‑1 (MQ=255) tgtCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGc < 2:404454/298‑1 (MQ=255) ttCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGccc < 2:850734/301‑1 (MQ=255) ttCATTACGCCGGAAGACGGCAGCAAAGACGTGGTCGTAAACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGccc < 1:275525/301‑1 (MQ=255) ttCAGTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAAGCCAGACTAATGGTTTTAACACTCGTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACTTCAGCAAGAATTCAAAACCCGCTTAATCAGCGT‑G‑TTTTTTGTGTTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGccc < 2:119647/301‑1 (MQ=255) cTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATCCCAACCCaaa > 1:322200/1‑301 (MQ=255) ccGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTACTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTGGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAACAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTTCGGTTACGGCCGAAAACAGCCAAACCGCCAGTGCGGTCATGGCACAAGACCCCAGAAGGTTGACGagacggttacttacttcccaccccaag > 2:377563/1‑274 (MQ=255) gcaAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTCAGACTCTTGCTTAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGCCCCTTCTGCTGCAACCGTAATCGTTCTGTACGATACGTAAGCAAGAATTCAAAACCCGCGTAATCAGCAG‑G‑TTTTTGTTGTTCTTTAGTGTGCGGTTGCGGACGAATACAGCCAGCATGCCGTTTCGGCCATTGACAAGGACCCCATCAGGTTGACTAAAGCGTCACGTAATgacagacacatacgcccaacttttaa > 1:677817/1‑276 (MQ=255) ttaagacgcttttgttatactttctGCAATGCACACTTATGGTTTTAAAACGCTTGCTGAAGGTCCGCGACTAGACTTCCCATTCACTAACTGGTCTAAAGCACCTTCTGCTGCAAACGTAATCTTTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCAG‑G‑TTTTTTTTGTGCGTTAGTGTGCGGTTGATGCCGAAAAGAGCCAGAATGCCAGTGTGGTCATGGCAAAAGCCCCCATAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAc < 1:312201/279‑1 (MQ=255) aagagtttgaaaagagacagcatctGAAATCAAGCCTAATGGTTTTAAAACCTTTGCGGAAGGTCAGGCCGAAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAc < 1:532005/279‑1 (MQ=255) gACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTCGAGGCCGAAAACCGCAAGAATACCAGTGCGGCCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATACCCAGCCAAAGCGGCCATCTTGTAACaaca > 2:566051/1‑299 (MQ=255) gtTCGTACCCTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTAGAACTCACTAACGATGCTAGAGGCGCTTCTGCTGCAAACGTAATCGCTCTGTACAATACCTCAGCAGGACTTCAAAACCAGCTTACTCCCCAG‑T‑TTTTTTTTGTTCTTTAGTGTTCGGTTGAGGCTGACAGCAGCCAGAATGCCAGTGCGGTAGTGGCAAACGACCCCAAGAGGTTGACGAAAAATGTCAGTTATAAACAGCCAACGCGGCACACTAGTAAAACAAacaca > 1:296342/1‑300 (MQ=255) ttCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAGTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGGGGAGGCCGAACACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCCGCAGGTTGACGAAAACGTTCAGTAAGGCCCAGCGAAAGCGGCACTATTGTAACAAAACcacca > 2:425336/1‑301 (MQ=255) tACACTTTTCTGCAATCCAGACCAATGGTTTTAAAACTCTTGCTGAAGGCCAGCGCGTAGAGGTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑‑TTTTTTTGGTCGTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTc < 2:848769/300‑1 (MQ=255) cTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTACTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGCCCCCAGAAGGTTGACGAAACGCTTCAGTACTGCCCAGCCAAAGAGGCCCACTTGTAACAAAACCACCACTTCTGc > 1:552968/1‑300 (MQ=255) tctcTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAACGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGACGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGa > 1:873139/1‑301 (MQ=255) tctcTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAc > 2:532021/1‑281 (MQ=255) ctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCAGCCCTCTTGTCACAAAAACACCAATTCTGCCGaaaa > 2:280338/1‑301 (MQ=255) aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTTTGCGGTTGAGGCCGCAAACAGCCAGAATGCCAGTGCGGTCATGGCAAACGACCCCAGAAGGTTTACAAAACAGTTCAGTAATTCCCAGCGAAACCGGCCCCCTTTTAACACAAACACCAATTCTCCCGAAAATGttt > 2:819133/1‑300 (MQ=255) aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGCAAACAGCCAGAATTCCAGTGCGGTCATGGCAACAGACCCCAGACGGTTGACGAAAACGTTCAGTAATGCCCCGCCAACGCGGCCCTCTTGTAACAAAAACACCACtttgtccgacaattttt > 1:654435/1‑286 (MQ=255) gACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCACGAATTCAAACCCCGCTTACTCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGCGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAATACCACAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCACAGCGGCCCTCTTGTACCAACAACACCCCTTCTGCCCAAAATGTTCAGAAg > 2:774500/1‑301 (MQ=255) gACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCCGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGACGAAAATTTTGAGAAg > 1:525472/1‑301 (MQ=255) aagaagggTTTGAAAACTCTTGCGGAAGTGTAGTGCGTAGAGTTCGAAATCACTAACGGTGCAAAAGGACCGTCTGCTGCAAACGTAATCGCGCGGTAAGATACGTCAGCAAGAATTCAAAACCTGCTGAATCAGCGG‑T‑TTTTTTTTGGTCTTGAGGGGGCGGTGGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTgaga < 2:296350/292‑1 (MQ=255) agaaagggtttAAAACGATGTCTGAGGGTCAGAGGGGAGAGGTAGAAATCACTCAGGGTGCCAAAGGGGCGTAAGCTGCAGACGGAATCGCGCTGGAGGATACGGCAGCAAGAATTCAAAACGCGCGTAAGCAGCGG‑T‑TTTATTGTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCGAAAGACCCCAGAAGGTTGAAGAAGACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAGAACGCCACTTGTGCCGAAAATGTTGAGAAgg < 1:29261/292‑1 (MQ=255) gTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTTCCACAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATCCGTCAGCAAGCATTCAAAACCCGCTTAATCCGCGG‑G‑TTTTTTTTGGTCTTTAGTTTGCGGTTGAAGCCGAAAACAGCCAGAATTCTAGTGCGGTCCTGGCAAACGACCCCAACATGTTGACGAAAACGTTCCGTAATGCCCCACCAAAGCGGCCCTCTTGTAAACAAAACCCCACTTCTCCCCACACAGTTGACGAGGTTTTTAg > 1:621987/1‑301 (MQ=255) gTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCCAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCCGACAAAGCGGCCCACTTGTAACAAAAACACACCTTCTGCCCAAACTGTTGAGACGGTTGTTaa > 1:29527/1‑300 (MQ=255) ttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTATTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCCGAAGGTTCACGAAAACGTTAAGTAATGCCCAGCAAAACCAGCCCCCTTGTAACAACACAACCCATTATTCCGAACATGTTAAGAAGGTTGGTAGAc > 1:469831/1‑300 (MQ=255) tGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTTCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCCCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAAt < 2:501871/301‑1 (MQ=255) ggTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGACAACAGCCAGAATGCCGGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACGACTTCTGCCGAAAATGTTGAGAGGGTTGTTAGACCGCCAACAAAACCggtgg > 2:144574/1‑301 (MQ=255) gtcctcgcgTTGAGTTCCAAAACCCCAACTGTTCCCAAGGCCCTTCTTCTGCAAACTTAATCCCACTTTAAGATACTTCAGCAAGAATTCAAAACCCGCTTAATCAGCTG‑T‑TTTTTTTTGGTCTTTAGTGTGCTGTTGAGGCCGAAAACAGCCAGAATGCCAGTTCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACCAAAACACCACTTCTGCCGACAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCggtgg < 1:312228/295‑1 (MQ=255) acgcgTAAAGTTCGAAAACAATAACGGTGCCAAAGGCCCCTCTGCTGCAAACGGAATCGCTCAGTAAGATACGTCAGCAAGAATTAAAAACCAGCTTAATCAGCGG‑G‑TTGTTTTTGGGCTTGAGGGTGCGGTTGAGGCCGAAAACATCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGAtt < 1:936438/300‑1 (MQ=255) ggcgTAGAGATCAAAATCCCTAACGGTCACAAACGCCCTTCTGCGGCAACCGTAATCGCTATGTAGGATACTGAAGCAAGAATTCAAAACCGACTTAATCAGCGG‑G‑TTTTTTTTGGGCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAATACCCCAGAAGGTTGACGAAAACGTGCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGAtt < 2:83561/299‑1 (MQ=255) tAGAGGTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGGGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATAc < 2:873168/301‑1 (MQ=255) agagTTAGAAATCAGTAACGGGGCCAAATGCACTTCTGGTGCAACAGTAATAGATCTGTAAGCACAGCCAGCAAGAATTCAAAACCCGCTTAATCAGCGC‑T‑TTTTTGTTGGGCTTTAGTGTGCGGTTGAGGCCGAAAACAGCAAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGGTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGACCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATAc < 2:600847/300‑1 (MQ=255) gagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAacac > 2:302936/1‑236 (MQ=255) gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGGTGTTAGACCGCCACAAAATCCggtgg > 2:126133/1‑287 (MQ=255) gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGGAATCGCTCTGTAAGAGACGTCAGCAAGAATTCAAAACCCGCTTAAGCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGGGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCggtgg < 1:126128/287‑1 (MQ=255) aaaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTATTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAc > 2:648513/1‑164 (MQ=255) aaaTCACTAACGGTGCCAAAGGCCCTGCTGCTGCAAACGGAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAc < 1:648485/164‑1 (MQ=255) cACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTTAGCAGGTTGTTAGACCGCCACACAAACCGCGTGTGATTAATACGTTCCACACTGGa > 2:171834/1‑301 (MQ=255) gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGCTACGTCAGCAAGAATTCAACACCAGCTTAATCGGCGGTT‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCCTGGCCAAAGACCGCATAAGGTTGCCGCAAACGTTCAGTAATGACACGCCAAAGCGGCCCACTTGTACCAAAACAACCCATTATGCCGATAATCTTTCGACAGTTGTTGGACAGCCACAAAATCCGGTGGTGCTGAATACTTTCCCCACTGGGTCatatt > 2:185966/1‑297 (MQ=255) gTGCAAAAGACCCTTATGGGGCAAACGTGTGCGGGCGGTAAGATACGTCAGTAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTGGGTCTTTAGTGTGCGGGTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACGTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATg < 1:603246/299‑1 (MQ=255) gCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAACACCCGCTTAATCAGCGG‑T‑TTTTTTTTTGTCTTTAGTGTGCGGTTGAGGCCGACAACAGCCAGAATGCCAGTGCGGTCATGGCAAACGAACCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCCGCCCAAGCGGCCCTCTTGTACCCACAACACCACTTCTGCCTAAAATGTTGAGACGgttgttc > 2:651752/1‑246 (MQ=255) ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAACACGTTCAGTCATGCCCAGCCAAAGCGGCCCTCTTGTAACACAAACACCCCTTCTGCCGAAAATCTTGAGAAGGTTTTTCGCCCGCCACAAACTCCTGTTGTGATTAATACTTTCCCCACTGGATCAATGTTTGt > 1:305473/1‑301 (MQ=255) aaaaGGCCCTTCTGCGGCAAACGTAATCGCTCTGTAAGATAAGTCAGCAAGAATTCAAAACCCGCTGAATCAGCGG‑T‑TTTGTTTGGGTCTGTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTc < 2:783060/300‑1 (MQ=255) aaaaGGCCCTTATGCTACAAAAGTAGTCAACTGGTAAGAGACGTCAGAAAGAATGCAAAAGCCGATGAAGCATCGG‑T‑TTTTTTTTGGTCTGTAGTGGGCGGTGAAGGCCGAAGACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTAAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAAGCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTc < 2:449758/300‑1 (MQ=255) aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGCATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTTAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGaat > 1:385810/1‑154 (MQ=255) ctgctgCAAACGGAATCGCTCTGGAAGATACGGCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTGTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAg < 2:560711/235‑1 (MQ=255) tgctggAAACGTAATCGCCCGGGAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAc < 2:525487/301‑1 (MQ=255) taatcgacgttgaaGATACGTCAGCAGGAAATGAAAACCCGCGTAATGAGTGG‑T‑TTTTGGTTGGTCTTTAGTGTGCGGGTGAGGCCGACAACAGCCAGAATGCCAGTGCGGTCATGGTAAAAGACCCCAGAAGGGTGACGACAACGGTCAGTAATGCCCATCCAAAGCGGTCCTCTTGTAACAAAAACACCACTGCTGCCGAAAAGGCTGAGAATGTTGTTCGACCGCCACAAGATCCGGTGGTGATTAATCCTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcc < 1:788828/289‑1 (MQ=255) tAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGTTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACCACATCCGGTGGTGATTACTACTTTCCCCACTGGATCACTGTTCGTCATACTGCTGAACCATGCGaacc > 1:168003/1‑298 (MQ=255) tctctGTAAGATACGGCAGCAAGAATTCAAAAACCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattc < 2:322211/300‑1 (MQ=255) gCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTGCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCACCACCAAATCCAGGGGTGATTAATACTTTCCACACTGCATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcatttt > 2:443852/1‑295 (MQ=255) ctgtttAACATACGGAAGCAAGAATGCAAAAACCCCTTAATCAGGGG‑T‑GTTTGTTGGGTCTTGAGTTTGCGGTTGAGGCCGAAAACAGCCAGAATGTCAGCGCGGCCATGGCAAAAGACCAAAGAAGGTTGACGAAAACGTTCAGTAATGCCCAACCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGTCGAAAATGTTGAGCAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAgcctattcc < 1:774472/296‑1 (MQ=255) ctctGTAAGATACTTCAGCAAGAAGTCCAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTGGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc < 2:469845/301‑1 (MQ=255) ctctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAGAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCCAAGATGCCCTCTTGTAACAAAAACACCACTTCTGCGGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGCAAGTAATAATTTCCACACTGGACCAATGTTCGTCTTCCTGCTGAACCCTCAGAAtcctattc > 1:818854/1‑300 (MQ=255) ctctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGGGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattc < 2:654463/300‑1 (MQ=255) ctcattAAGATAAGCGAGCAATAATTCAAAACCCGCTCAATCAGCTG‑G‑TTTTTTTTTGGCGTTAGTGAGCGGTTGAGGCCAATAACAGCCAGAATTCCAGTGCGTTCAGGGCAAAAGACACCAGAAGGTTGACTAAAACGTTCAGTAATGCCCAGCTAAAGCGGCCCTCTTGTAACAAAAACACAACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc < 1:843683/296‑1 (MQ=255) tctGTAAGATACGGAAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc < 2:552984/300‑1 (MQ=255) tgtgaaGATAAGGCAGTAAGAATTAAAAACAATGTTAATCAGCGG‑T‑GTTTGTTTGGTCTTTAGTGTTGGGTTGAGGTCGACAACAGTCATAAGGCCAGTGCGGTAAGGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattccta < 2:783480/297‑1 (MQ=255) ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCCCAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattccta > 2:86974/1‑301 (MQ=255) ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTGTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAg < 2:339318/215‑1 (MQ=255) ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCACAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAACAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCTCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCCGCTACACCATGCGAAtcctattccaa > 2:604819/1‑299 (MQ=255) ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCAGATGGTGATTAATCCTTTCCACACTGGACCAATGTTCGTACTCCTCCTGAACCATGCGACtcatattccta > 1:333704/1‑301 (MQ=255) gTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGCAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATAATTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTACTatg > 2:743152/1‑301 (MQ=255) gTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑GTTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAACGACCCAAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAACAACCACTTCTGCCGAAAATGTTGCGCATGTTGTTAGACCGCCCCAACATCAGGTGGTGATTAATACTTTCCCCACTGGCTCAATGTTGGTCATCCTGCTGAACCATACGAAtcctcttcctat > 2:817634/1‑301 (MQ=255) aGATACGTCAGCAAGAATTCACAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTACGGTTCAGGCCGAAAAGAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCACCGCCAACGCGGCCCTAGTTTAACAAAAACAACACTTCTGCCTAAAATGTTGAAAAGGTTGTTAGACCGCCACAAAATCAGGTGTTGCTTAATAATTTCCGCGCGGGAACATTGTTCGTCAGACTTGTGAAACAATCGAAtcctcttcctattagt > 1:530524/1‑297 (MQ=255) atagggcAGAAAGAATTAAAAACCCGCTTAAACAGCGG‑T‑GTGTTTTTGGTCGTTAGTGGGCGGTTGAGGCCGAAAACAGCCAGAAGGCCAGGGCGGGCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatgatg < 1:743123/293‑1 (MQ=255) aTACGTGAGCAAGAATTCAAAAACCGAGTAATCAGCGG‑G‑TGTGTTTGGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatgatg < 2:380684/299‑1 (MQ=255) cAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑T‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCCCAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGACTGccc > 2:558037/1‑300 (MQ=255) cAAGGATTCAAAACCCGCGTAATCAGCGT‑G‑TTTTTTTTGGTCTTTAGTGTTGGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatgatg < 2:206552/290‑1 (MQ=255) cAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGACAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGAGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATGAATCATTTCCAAACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATCCCTatgatgctgtttattaa > 1:206545/1‑290 (MQ=255) tttAATCAGCTG‑T‑TTTTGTTGGGTCTGTAGTGTGCGGGGGATGCCGAAAACAGCCAGAATGCCGGTGCGGTCATGGCAAAAGACCCCAGAATGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGGAACAACAACGCCACTTCTGCCGAAAATGTGGAGAAGTGTGTTAGACCGCCACAAAATCCGGTGGTGATTAAGACTTTCCACACTGGATCAATGTTCGTCTTCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTTCTGTCAGCg < 2:440950/300‑1 (MQ=255) cgtg‑TTTTTTTTGGTCTTTAGTGTGCGGTGGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAATAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAAc < 2:818882/297‑1 (MQ=255) | GGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGG‑G‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAAC > NC_000913/657243‑657837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |