Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,458,7531.→G 1.1% 0.8 0.0 ‑0.0180coding (415/1482 nt)gspEgeneral secretory pathway component, cryptic
Rejected: E-value exceeds prediction threshold.
Rejected: Polymorphism does not pass frequency cutoff.
Rejected: Polymorphism prediction not supported by minimum number of reads on both strands.
Reads supporting (aligned to +/- strand):  new base (1/1):  ref base (87/91):  total (88/92)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.07e-01

Only 100 of 183 total aligned reads displayed.

ATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATC  >  NC_000913/3458454‑3459047
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                      
atatTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑g                                                                                                                                                                                                                                                                                                       >  1:40797/1‑301 (MQ=255)
atatTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑g                                                                                                                                                                                                                                                                                                       <  1:751675/301‑1 (MQ=255)
  atTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGAGCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCAATCAAAGAAACCGCCCCTAATATCAACATTGAAACCTATGAAAAAACAATTTCGAAAC‑Gtt                                                                                                                                                                                                                                                                                                     >  2:81958/1‑301 (MQ=255)
       aCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCAACGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCGATGAACAAACCATTTCGATCC‑GTTTTCg                                                                                                                                                                                                                                                                                                 >  2:590687/1‑300 (MQ=255)
            aGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTAGCATCg                                                                                                                                                                                                                                                                                            >  1:876650/1‑300 (MQ=255)
              gTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGAc                                                                                                                                                                                                                                                                                          <  1:693079/300‑1 (MQ=255)
                tGGCCAGGGCCAGACAATGACCAGCTTGTCACAGGCTGATTTTGAAGATCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAAAAGATTGCGCAGGATATCGATCAAGCCGTCGATTTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATAGCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACgg                                                                                                                                                                                                                                                                                        <  2:736776/300‑1 (MQ=255)
                      gTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCCCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGCAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTg                                                                                                                                                                                                                                                                                 >  2:71908/1‑301 (MQ=255)
                      gTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGCTGAGTCGCTACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCGCTCGCTGTCGGAAGAGATTCCCGCAAATGAATATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCAGCTTGATCAATGCGCTGTTGAGTGATGCCATCAAAGAAACCGCCTGTGATGTCCCCCTTCAACCCTATGTAAACAACATGCAGATCC‑GTTTTCGCATCCACCGccattt                                                                                                                                                                                                                                                                                  >  2:742765/1‑295 (MQ=255)
                                aTGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAAttt                                                                                                                                                                                                                                                                       >  2:120442/1‑301 (MQ=255)
                                   aCCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCGGGAAGCGGTATTCCTGCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAAGCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTAc                                                                                                                                                                                                                                                                    <  1:6556/301‑1 (MQ=255)
                                       gCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAACGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGc                                                                                                                                                                                                                                                                 >  2:279610/1‑300 (MQ=255)
                                           gTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAACAAATGTCGATCC‑GTTTTCGCATCGCCGGCGTTTTGCGGACAATTTTACAGAcaaa                                                                                                                                                                                                                                                             >  2:630498/1‑300 (MQ=255)
                                           gTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAACAAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCcaaaa                                                                                                                                                                                                                                                            >  1:24403/1‑300 (MQ=255)
                                           gTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCcaaaa                                                                                                                                                                                                                                                            >  1:198003/1‑300 (MQ=255)
                                           gTCACAGGCGGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCACCAGATTTCGCAGGATATAGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCCATGCGATTGTGAGTGCGGCCGTCAAAGAAACCGCCTCTGATATCCACATTGAACCCACTGAACCAACAATGTCAAACA‑GTTTTCGCACCGACGGCGCATTGCGAACACTTTGACACCcaca                                                                                                                                                                                                                                                             >  2:9309/1‑298 (MQ=255)
                                                                    cTGGAAGAGGTACTCCATCAGAATACCGGTGAGTCGCAACAGATGGAGCAGGAGATCGATCAATCCGTCGATCATCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTAtc                                                                                                                                                                                                                                    <  2:83240/300‑1 (MQ=255)
                                                                              tATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTa                                                                                                                                                                                                                          <  1:120445/300‑1 (MQ=255)
                                                                                ttCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAgg                                                                                                                                                                                                                       <  1:768860/301‑1 (MQ=255)
                                                                                     tCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGCCAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATg                                                                                                                                                                                                                  >  2:117968/1‑301 (MQ=255)
                                                                                     tCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCAtt                                                                                                                                                                                                                  >  1:966918/1‑300 (MQ=255)
                                                                                     tCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATg                                                                                                                                                                                                                  >  2:641291/1‑301 (MQ=255)
                                                                                     tCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTAGCTCCCGAATTAGGGTCATg                                                                                                                                                                                                                  >  2:421591/1‑301 (MQ=255)
                                                                                        gAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGCAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACACTTTTACAGCCAAACAAACAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGc                                                                                                                                                                                                                >  1:647638/1‑300 (MQ=255)
                                                                                        gAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGACAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTTCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGc                                                                                                                                                                                                                >  1:215907/1‑300 (MQ=255)
                                                                                        gAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGc                                                                                                                                                                                                                >  1:601620/1‑300 (MQ=255)
                                                                                        gAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAAAGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGc                                                                                                                                                                                                                >  2:921256/1‑300 (MQ=255)
                                                                                             ccGGTGAGTCGCAACAGATTGCTCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCt                                                                                                                                                                                                          <  2:59994/301‑1 (MQ=255)
                                                                                                 tGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGat                                                                                                                                                                                                      <  1:400553/301‑1 (MQ=255)
                                                                                                   tgTCGCAACATATTGCGCAGGATCTCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGGGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGatat                                                                                                                                                                                                    <  2:80762/300‑1 (MQ=255)
                                                                                                   aGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGatat                                                                                                                                                                                                    >  1:430302/1‑301 (MQ=255)
                                                                                                   aGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGata                                                                                                                                                                                                     <  1:890589/300‑1 (MQ=255)
                                                                                                   aGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGata                                                                                                                                                                                                     <  1:71910/300‑1 (MQ=255)
                                                                                                       gCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACAGCCTCTGATATCCACATTGAAACATATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCAAGGCTCGTCTTGATATCGc                                                                                                                                                                                                 >  2:681272/1‑300 (MQ=255)
                                                                                                          aCAGACTGCGCAGGATATCTATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTAGCCGCTTGATCAATGCGATGTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGt                                                                                                                                                                                                                      <  1:739138/276‑1 (MQ=255)
                                                                                                                 gcgcAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGAAATCGCCGAAAAACGTa                                                                                                                                                                                      >  1:44509/1‑301 (MQ=255)
                                                                                                                        atatCGACCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCcac                                                                                                                                                                                <  1:813761/300‑1 (MQ=255)
                                                                                                                         tatCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAg                                                                                                                                                                              <  1:432787/301‑1 (MQ=255)
                                                                                                                            cGATCAACTCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGAt                                                                                                                                                                           <  2:889072/301‑1 (MQ=255)
                                                                                                                                 aaTCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGgaag                                                                                                                                                                      <  1:630514/301‑1 (MQ=255)
                                                                                                                                  aTCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGgaaga                                                                                                                                                                     >  1:382216/1‑301 (MQ=255)
                                                                                                                                   tCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCAAAGGATGgaagaa                                                                                                                                                                    >  2:43672/1‑301 (MQ=255)
                                                                                                                                      gTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCCAAAAACGTTTTCCACAGGATGGAATACTTa                                                                                                                                                                 >  2:163077/1‑301 (MQ=255)
                                                                                                                                        cGATCTTTGCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGATTGAAGATTGAGCGGCACCGGTTATCCGCTTGATCACTGCGATTTTGAGTGAAGCCATCAAAGAAACCGACTCTGATATCCACATTGAAACCTAGGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAg                                                                                                                                                                <  2:459704/300‑1 (MQ=255)
                                                                                                                                         taTCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGtt                                                                                                                                                              <  1:301367/300‑1 (MQ=255)
                                                                                                                                          aTCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGtt                                                                                                                                                              <  1:482844/300‑1 (MQ=255)
                                                                                                                                              tctctcGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGat                                                                                                                                                                                                      <  1:361153/256‑1 (MQ=255)
                                                                                                                                                     cTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGata                                                                                                                                                                                                     <  2:476121/250‑1 (MQ=255)
                                                                                                                                                             agagATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTaaa                                                                                                                                          >  1:287778/1‑300 (MQ=255)
                                                                                                                                                                        cAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACAt                                                                                                                                        <  1:193159/292‑1 (MQ=255)
                                                                                                                                                                          aaTGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGgaag                                                                                                                                                                      <  1:446439/260‑1 (MQ=255)
                                                                                                                                                                             gAAGATCTCCTGAATGAAGATTCAGTGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAAc                                                                                                                                                                                                                                                     <  2:241223/178‑1 (MQ=255)
                                                                                                                                                                             gAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGCATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGGATTAGTTTGCGTTTCGGGCGACCTAACATAGATGTc                                                                                                                                 >  2:5016/1‑294 (MQ=255)
                                                                                                                                                                             gAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTc                                                                                                                                 <  1:5016/294‑1 (MQ=255)
                                                                                                                                                                                   ctcCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCacac                                                                                                                    <  2:40796/301‑1 (MQ=255)
                                                                                                                                                                                        gaatgaaGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCAAAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGGATACacacgtccg                                                                                                               >  2:768155/1‑296 (MQ=255)
                                                                                                                                                                                           tgacGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGAGTTGGAATGAGGCTATCAAAGAAACCGTCTCTGATATCCACATGGAAACCTATGAAAAAACAATGTCGATCC‑GGTTTCGCATCGACGTCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTCTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTcc                                                                                                            <  2:647614/301‑1 (MQ=255)
                                                                                                                                                                                           tgaagagtacgcgtgaCCGGGTATCCGCTTGATTAATGCGATTTGGAGTGATGCAATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGAGCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTcc                                                                                                            <  2:215906/286‑1 (MQ=255)
                                                                                                                                                                                             aagatcaggCGGCACCGGTTATCCGCTTGATAAATTCGATTTTGAGTAAGGCCATCAAAGAAAACGCCTCTGATATCCACATTGAAACCTAGGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCAt                                                                                                          <  1:279020/293‑1 (MQ=255)
                                                                                                                                                                                              aGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGGTGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTTcctttcct                                                                                                          >  2:34074/1‑294 (MQ=255)
                                                                                                                                                                                                tttCAGCGGCCCGGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCAACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGAGATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTa                                                                                                       <  2:549359/300‑1 (MQ=255)
                                                                                                                                                                                                      cGGCACCGGTTATCCGCTTGATAAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCAACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTAATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCGGCGTATCCACACTGCCGTCCATCTATgg                                                                                                    <  1:472692/298‑1 (MQ=255)
                                                                                                                                                                                                         cACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAAc                                                                                               <  2:287777/300‑1 (MQ=255)
                                                                                                                                                                                                           ccGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAAcgcg                                                                                            >  1:469455/1‑301 (MQ=255)
                                                                                                                                                                                                              gTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTa                                                                                                       <  1:203467/287‑1 (MQ=255)
                                                                                                                                                                                                              gTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCAAAGGATGGAAGAATTAGTTTGCGTATCGGGCGAAGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTa                                                                                                       >  2:203466/1‑287 (MQ=255)
                                                                                                                                                                                                                  cccgcattaacAATGCGAATTTGAGGTAAGACATAAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAAGGTCTCTCC‑GTTTTCTCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTAGCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACt                                                                                     <  2:876609/291‑1 (MQ=255)
                                                                                                                                                                                                                   ccGCTTGATCAATGCGATTTTGATGGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTc                                                                                    <  1:12763/301‑1 (MQ=255)
                                                                                                                                                                                                                   ccGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTc                                                                                    <  1:641315/301‑1 (MQ=255)
                                                                                                                                                                                                                      ttttaTCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGc                                                                                 <  2:469448/297‑1 (MQ=255)
                                                                                                                                                                                                                         gATCAATGCGATTTTGGGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGata                                                                                                                                                                                                     <  2:470795/182‑1 (MQ=255)
                                                                                                                                                                                                                         gATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGc                                                                                 >  1:423647/1‑298 (MQ=255)
                                                                                                                                                                                                                                 cGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATaa                                                                      <  1:43673/301‑1 (MQ=255)
                                                                                                                                                                                                                                   aTTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATaaaa                                                                    >  1:538544/1‑301 (MQ=255)
                                                                                                                                                                                                                                       tgcgtgagGCCATCAAAGAAACCGCCTCTGATATCCAAATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAg                                                                <  2:44508/298‑1 (MQ=255)
                                                                                                                                                                                                                                           tgagGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCt                                                             <  1:513951/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 aaTCAAAGAAACCGCCCTTGATATCCACATTGAAACCTTTGAAAAAACAATGTCGTTCC‑GTTTTCGCATGGAAGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAGACTGGCGGCACTGCTTATCACCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCtt                                                      <  2:198000/300‑1 (MQ=255)
                                                                                                                                                                                                                                                      aaGAACCCGCCTCTGATATCCACATTGAAACCGATGAAAAATCAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGAt                                                                                                                                                                           <  2:137854/179‑1 (MQ=255)
                                                                                                                                                                                                                                                       aGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCAt                                                                                                          <  1:824173/243‑1 (MQ=255)
                                                                                                                                                                                                                                                          aaCCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGaac                                             <  1:117971/301‑1 (MQ=255)
                                                                                                                                                                                                                                                          aaCCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGaac                                             <  2:490905/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ctGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACACAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTCTCGGGCGACGTAAACTAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGGGAACGCGCCGTACTCCGACTGCGGGATAAAAACAGCCTCCAGCTTTCATTGCACAACCAggg                                     >  2:251605/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                  ctGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACa                                                                                                                                         >  1:932552/1‑201 (MQ=255)
                                                                                                                                                                                                                                                                    tagatCCACATCGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACAt                                                                                                                                        <  2:908200/197‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCTCTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATTGAAGAATTAGTTTGCGTATCGGGCGACGTAACAt                                                                                                                                        >  1:908243/1‑200 (MQ=255)
                                                                                                                                                                                                                                                                    aatatCCAAATTGAAACCTATGAAAAAACAATGTCGATCC‑GGTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGa                                   <  1:163080/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                     atatCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGa                                   <  2:367713/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                     atatCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGa                                   <  1:775771/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                     atatCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGAATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGa                                   <  1:897952/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                     atatCCACATTGAAACCTATGAAAAAACAAAGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGa                                   <  1:921304/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                            caTTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTCCGTATCGTGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCAATCTATGGCGAACGCGCCGTACTCCGCCTGCTGGATAAAACAAGCCTCCAGCTCTCATTGAACAACCTGGGGATGACGGCa                           >  1:457119/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                      tATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGc                 <  2:153044/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       aTGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATg                                                                                                                                   <  2:431316/186‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         aaaaaaaCAATGTCGATCCGGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACCGCCAAACAACAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAg               <  2:372160/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             aaaCAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCacac                                                                                                                    <  1:294952/195‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             aaaCAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCacac                                                                                                                    >  2:294951/1‑195 (MQ=255)
                                                                                                                                                                                                                                                                                             aaaCAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAAACACCTCCAGCTTTCATTTCACAACCTCGGGATGACGGCCGCGGATGAGCAGGAttt          >  2:276928/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                cAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGa       <  1:368445/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                    gTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGaaaaa   >  1:150342/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                    gTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAAt   >  1:787221/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                      
ATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATCGATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACCGGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAAAAACAATGTCGATCC‑GTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTTATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTATCGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATAAAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATC  >  NC_000913/3458454‑3459047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.