Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,187,140	0	T→G	100.0%	0.7	3	F211V (TTC→GTC)	insD1	IS2 transposase TnpB
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/2):  ref base (1/0):  total (1/2)

AAACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATC  >  NC_000913/3187129‑3187435            |                                                                                                                                                                                                                                                                                                        aaaCACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATCGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTACTGGGTTAAGTGATCACAGCTGTCTGGAAATATAGCGGCCAATACAGtt        >  1:810142/1‑301 (MQ=2)     ccGCCAGGTCGCCCGGGGGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGGGTCCGGAGAGGAACGGAATAGCAGAGAGCGTCGTGAAAACGAGAAAGCGTGACTACATCAGTATCAGGCCCAAACAAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTatca    <  1:661173/300‑1 (MQ=255)       cccAGGTAGCCCGTATTTTGGGCATTCAGCCGACGAACACGGCGGTGCGGTGTCCAGAGAGTAACGTAATAGCATAGAGTGTCGTGAAAAAGGTAAAGCGTGACTACATCAGTATCATGCCCAAACCCGACTGGTTAAAGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCAGTATAACGAATGGCCTCCGCATAGTGCGCTGGGTTAGCTCTCGACACGGGAATATCGGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTagcatc  <  1:721388/300‑1 (MQ=11)            |                                                                                                                                                                                                                                                                                                        AAACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATC  >  NC_000913/3187129‑3187435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.