Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009131,299,5260A→G100.0% 0.9 2intergenic (+281/‑1656)ychE/oppAUPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/2):  ref base (0/0):  total (0/2)

ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGAAACCATGCTACGCATTCACTGCATGCAGCATTGGTACAACC  >  NC_000913/1299463‑1299780
                                                               |                                                                                                                                                                                                                                                              
taaaaacaaactgattgagaaaaagGAAAGTAAGAAGGAATGTGGGAACAAGGCCGGGAAATGGGGTCAAGTGTGTAAACTGGAGACATAGAAAAGGGGTAAACATGAGTCATAAAATGACCTTCACCGACAGTGAATTCAGCAGTAAGCGCCAGCAGACCAGAAAAGAGATGTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGAAACCATGCTACGCATTCACtgcat                   <  1:855781/278‑1 (MQ=11)
                  tataaggtacaatGCGAAGGAAGCGGCGAAAAAGGCCCTGATATTGGAGATTGTCTTTCATCTGGAGACAGACAACATGTTTAATCACGAGTCATCAACGTACCGTGGTGTACAGTGAAGTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGAGTCTGCAATGGTAAAACATGGGGGAAGTCATCGAGCCGGTTTACCCCAAGGCTGGGAATGGCCGGCGACCGTAGCCGCTGGAAACCATGCTACGCATTCACTGCATGCAGCATTGGTACAAcc  <  1:1344604/288‑1 (MQ=11)
                                                               |                                                                                                                                                                                                                                                              
ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGAAACCATGCTACGCATTCACTGCATGCAGCATTGGTACAACC  >  NC_000913/1299463‑1299780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.